Monoclonal antibodies are essential for the peptide immunoaffinity enrichment step prior to multiple reaction monitoring-mass spectrometry (MRM-MS) assays. But anti-peptide antibody generation using traditional mouse hybridoma methods has limitations, such as long timelines, uncertain success rates and the inability to control selection parameters such as affinity.
We collaborated with scientists at the Fred Hutchinson Cancer Research Center to carry out a feasibility study to discover whether recombinant anti-peptide antibodies generated using the HuCAL® antibody library and phage display technology are suitable for immunoaffinity enrichment of peptides coupled to targeted MRM.
Presented by: Dr Christian Frisch, R&D Manager, Bio-Rad Laboratories
Christian Frisch is R&D manager at Bio-Rad and has led the antibody generation group since 2003. He studied molecular biology in Göttingen (Germany) and did his Ph.D. with Prof. Alan Fersht at the University of Cambridge (UK) working on the energetics of protein-protein interactions and protein folding. In 1997 he joined MorphoSys AG, working on the development of display technologies and the generation of antibody phage display libraries. His group at Bio-Rad has generated more than 25,000 antibodies for custom projects over the last 13 years.