F4/80 Antibody: Mouse Macrophage and Microglial Marker
The Best Known Mouse Microglia and Macrophage Marker
"Bio-Rad produces most cited physiology antibody [F4/80 Clone CI:A3-1]"
F4/80 antibody - clone CI:A3-1 (MCA497), is a well-characterized and extensively referenced mouse macrophage marker. Also known as Ly71 and EMR1, the F4/80 antigen is part of the EGF-TM7 family, it is a 160 kDa glycoprotein that shares 68% amino acid identity with human EGF-like module-containing mucin-like hormone receptor-like 1 (EMR1).
F4/80 antigen is a mature mouse cell surface glycoprotein expressed at high levels on various macrophages including: Kupffer cells, splenic red pulp macrophages, microglia, gut lamina propria, and Langerhans cells in the skin. F4/80 antigen is also expressed on the macrophages of the connective tissue, heart, kidney, reproductive, and neuroendocrine systems.
Did You Know?
F4/80 antibody (MCA497, clone CI:A3-1) is the original and the best:
- High specificity - shown to be highly targeted for all mouse macrophages
- Added versatility for microglial cells - unlike many other macrophage markers F4/80 is present on microglia which makes it perfect for detecting and isolating these cells in neurological research
- Original with pedigree - the anti F4/80 antibody is derived from Clone CI:A3, this clone is the original anti F4/80 antigen with an established heritage going back to 1981 when data was first published. Since then there have been hundreds of citations, with over 900 listed on CiteAb
F4/80 Antibodies at a Glance
The most popular F4/80 antibody (MCA497) options at a glance:
Species |
Clone |
Applications |
Format |
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*For IHC paraffin sections antigen retrieval techniques are recommended to achieve optimum IHC staining for F4/80. The F4/80 antigen can be masked by prolonged formalin-fixation and the paraffin-embedding process.
Other Related Macrophage Markers by Species Reactivity
In addition to CD68, Bio-Rad also offers a wide range of other macrophage markers such as:
Anti-Mouse Antibodies
Anti-Human Antibodies
Anti-Rat Antibodies
References
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McKnight AJ et al. (1996). Molecular cloning of F4/80, a murine macrophage-restricted cell surface glycoprotein with homology to the G-protein-linked transmembrane 7 hormone receptor family.
J Biol Chem. 5;271(1):486-9. -
Schaller E et al. (2002). Inactivation of the F4/80 glycoprotein in the mouse germ line.
Mol Cell Biol. 22(22):8035-43. -
Lin HH et al. (2005). The macrophage F4/80 receptor is required for the induction of antigen-specific efferent regulatory T cells in peripheral tolerance.
J Exp Med. 201(10):1615-25. Epub 2005 May 9.
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