The best known mouse microglia and macrophage marker
F4/80 Antibody Mini Review - The marker and the antibody
F4/80 antibody clone CI:A3-1 (MCA497), is a well-characterized and extensively referenced mouse macrophage marker. Also known as Ly71 and EMR1, the F4/80 antigen is part of the EGF-TM7 family, it is a 160 kDa glycoprotein that shares 68% amino acid identity with human EGF-like module-containing mucin-like hormone receptor-like 1 (EMR1).
F4/80 antigen is a mature mouse cell surface glycoprotein expressed at high levels on various macrophages including: Kupffer cells, splenic red pulp macrophages, microglia, gut lamina propria, and Langerhans cells in the skin. F4/80 antigen is also expressed on the macrophages of the connective tissue, heart, kidney, reproductive, and neuroendocrine systems.
F4/80 antibody (MCA497, clone CI:A3-1) is the original and the best:
Most popular F4/80 antibody (MCA497) options at a glance, click here to view all F4/80 antibodies.
*For IHC paraffin sections antigen retrieval techniques are recommended to achieve optimum IHC staining for F4/80. The F4/80 antigen can be masked by prolonged formalin-fixation and the paraffin-embedding process.
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In addition to F4/80, Bio-Rad also offers a wide range of other macrophage markers such as:
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