F480 antibody, mouse macrophage and microglial marker

F4/80 Antibody

The best known mouse microglia and macrophage marker

"Bio-Rad produces most cited physiology antibody [F4/80 Clone CI:A3-1]" CiteAb

F4/80 Antibody Mini Review - The marker and the antibody

F4/80 Antibody Mini Review - The marker and the antibody

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F4/80 antibody (MCA497), clone CI:A3-1, is a well-characterized and extensively referenced mouse macrophage marker. Also known as Ly71 and EMR1, the F4/80 antigen is part of the EGF-TM7 family, it is a 160 kDa glycoprotein that shares 68% amino acid identity with human EGF-like module-containing mucin-like hormone receptor-like 1 (EMR1).

F4/80 antigen is a mature mouse cell surface glycoprotein expressed at high levels on various macrophages including: Kupffer cells, splenic red pulp macrophages, microglia, gut lamina propria, and Langerhans cells in the skin. F4/80 antigen is also expressed on the macrophages of the connective tissue, heart, kidney, reproductive, and neuroendocrine systems. 

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F4/80 antibody (MCA497, clone CI:A3-1) is the original and the best:

  • High specificity -  shown to be highly targeted for all mouse macrophages
  • Added versatility for microglial cells - unlike many other macrophage markers F4/80 is present on microglia which makes it perfect for detecting and isolating these cells in neurological research
  • Original with pedigree - the anti F4/80 antibody is derived from Clone CI:A3, this clone is the original anti F4/80 antigen with an established heritage going back to 1981 when data was first published. Since then there have been hundreds of citations, with over 900 listed on CiteAb

F4/80 antibodies at a glance

Most popular F4/80 antibody (MCA497) options at a glance, click here to view all F4/80 antibodies.

Species

Clone

Applications

Format

Mouse
CI:A3-1
Flow Cytometry
IHC*
FITC
RPE
Purified
Alexa Fluor ® dyes
Biotin
APC
Low endotoxin
Pacific blue ™

*For IHC paraffin sections antigen retrieval techniques are recommended to achieve optimum IHC staining for F4/80. The F4/80 antigen can be masked by prolonged formalin-fixation and the paraffin-embedding process.


Explore F4/80 antibody images by application

IHC

f4/80 antibody immunohistochemistry

IHC

Featured references of F4/80 antibody

featured references of f4/80 antibody
  1. McKnight AJ et al. (1996). Molecular cloning of F4/80, a murine macrophage-restricted cell surface glycoprotein with homology to the G-protein-linked transmembrane 7 hormone receptor family.
    J Biol Chem. 5;271(1):486-9.
  2. Schaller E et al. (2002). Inactivation of the F4/80 glycoprotein in the mouse germ line.
    Mol Cell Biol. 22(22):8035-43.
  3. Lin HH et al. (2005). The macrophage F4/80 receptor is required for the induction of antigen-specific efferent regulatory T cells in peripheral tolerance.
    J Exp Med. 201(10):1615-25. Epub 2005 May 9.

View all anti-mouse F4/80 references


Other related macrophage markers by species reactivity

In addition to F4/80, Bio-Rad also offers a wide range of other macrophage markers such as:

Anti-Mouse Antibodies

Anti-Mouse Antibodies

Anti-Human Antibodies

Anti-Human Antibodies

Anti-Rat Antibodies

Anti-Rat Antibodies

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