F4/80 Antibody: Mouse Macrophage and Microglial Marker
The Best Known Mouse Microglia and Macrophage Marker
"Bio-Rad produces most cited physiology antibody [F4/80 Clone CI:A3-1]"
F4/80 antibody - clone CI:A3-1 (MCA497), is a well-characterized and extensively referenced mouse macrophage marker. Also known as Ly71 and EMR1, the F4/80 antigen is part of the EGF-TM7 family, it is a 160 kDa glycoprotein that shares 68% amino acid identity with human EGF-like module-containing mucin-like hormone receptor-like 1 (EMR1).
F4/80 antigen is a mature mouse cell surface glycoprotein expressed at high levels on various macrophages including: Kupffer cells, splenic red pulp macrophages, microglia, gut lamina propria, and Langerhans cells in the skin. F4/80 antigen is also expressed on the macrophages of the connective tissue, heart, kidney, reproductive, and neuroendocrine systems.
Did You Know?
F4/80 antibody (MCA497, clone CI:A3-1) is the original and the best:
- High specificity - shown to be highly targeted for all mouse macrophages
- Added versatility for microglial cells - unlike many other macrophage markers F4/80 is present on microglia which makes it perfect for detecting and isolating these cells in neurological research
- Original with pedigree - the anti F4/80 antibody is derived from Clone CI:A3, this clone is the original anti F4/80 antigen with an established heritage going back to 1981 when data was first published. Since then there have been hundreds of citations, with over 900 listed on CiteAb
F4/80 Antibodies at a Glance
The most popular F4/80 antibody (MCA497) options at a glance:
Species |
Clone |
Applications |
Format |
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*For IHC paraffin sections antigen retrieval techniques are recommended to achieve optimum IHC staining for F4/80. The F4/80 antigen can be masked by prolonged formalin-fixation and the paraffin-embedding process.
Other Related Macrophage Markers by Species Reactivity
In addition to CD68, Bio-Rad also offers a wide range of other macrophage markers such as:
Anti-Mouse Antibodies
Anti-Human Antibodies
Anti-Rat Antibodies
FAQs
What does the F4/80 antibody (clone Cl:A3-1) target?
F4/80 antibody, clone Cl:A3-1, recognizes F4/80 — also known as EMR1, ADGRE1, or Ly71 — a 160 kDa glycoprotein expressed on the surface of mouse macrophages. It's considered the definitive marker for identifying mouse macrophage populations.What species does this antibody react with, and what is the host species?
The antibody reacts with mouse tissue and is raised in rat, making it a rat anti-mouse antibody (isotype IgG2b).Which applications is F4/80 antibody (MCA497) validated for?
Flow cytometry, immunofluorescence, immunohistology (frozen, paraffin, and resin sections), immunoprecipitation, western blotting, immuno-electron microscopy, functional assays, and radio-immunoassays.What conjugate options are available for clone Cl:A3-1?
The clone is available in purified (unconjugated) form as well as FITC, RPE, APC, biotin, Alexa Fluor dyes, StarBright Dyes, Pacific Blue, and Amethyst Orange conjugates, plus a low-endotoxin purified format — 47 formats in total across conjugates and pack sizes.How well-validated is this antibody in published research?
It's extensively cited, with over 900 published citations tracked on CiteAb, and the clone's characterization dates back to 1981, making it one of the most established macrophage markers in the field.References
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McKnight AJ et al. (1996). Molecular cloning of F4/80, a murine macrophage-restricted cell surface glycoprotein with homology to the G-protein-linked transmembrane 7 hormone receptor family.
J Biol Chem. 5;271(1):486-9. -
Schaller E et al. (2002). Inactivation of the F4/80 glycoprotein in the mouse germ line.
Mol Cell Biol. 22(22):8035-43. -
Lin HH et al. (2005). The macrophage F4/80 receptor is required for the induction of antigen-specific efferent regulatory T cells in peripheral tolerance.
J Exp Med. 201(10):1615-25. Epub 2005 May 9.
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