CD200 Receptor 1 antibody | OX-102
Mouse anti Rat CD200 Receptor 1:FITC
Mouse anti Rat CD200 Receptor 1:Low Endotoxin
Mouse anti Rat CD200 Receptor 1
Mouse anti Rat CD200 Receptor 1:RPE
- Product Type
- Monoclonal Antibody
- Clone
- OX-102
- Isotype
- IgG1
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| MCA1959F | F | 0.1 mg | ||||
| MCA1959EL | C F FN IP WB | 0.5 mg | ||||
| MCA1959GA | C F IP | 0.1 mg | ||||
| MCA1959 | C F IP WB | 0.25 mg | ||||
| MCA1959PE | F | 100 Tests |
Mouse anti Rat CD200 Receptor 1 antibody, clone OX-102 recognizes the rat OX2 (CD200) receptor 1. This antigen is a heavily glycosylated ~60-100 kDa cell surface molecule expressed by cells of the myeloid lineage but not by T or B lymphocytes.
Mouse anti Rat CD200 Receptor 1 antibody, clone OX-102 has been shown to block the interaction of OX2 receptor 1 with CD200 (Bushell et al. 2008).
Mouse anti Rat CD200 Receptor 1 antibody, clone OX-102 has been shown to block the interaction of OX2 receptor 1 with CD200 (Bushell et al. 2008).
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- None present
- Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Membrane fraction of thioglycollate-elicited rat peripheral cells.
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at -20oC only.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 12 months from date of reconstitution.
More Information
- UniProt
- Q9ES58 Related reagents
- Entrez Gene
- Cd200r1 Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- Regulatory
- For research purposes only
Applications of CD200 Receptor 1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | |||
| Functional Assays | |||
| Immunohistology - Frozen | 1/10 | 1/200 | |
| Immunoprecipitation | |||
| Western Blotting | |||
| Flow Cytometry | |||
| Immunohistology - Frozen | |||
| Immunoprecipitation | |||
| Western Blotting | |||
| Flow Cytometry | Neat | 1/10 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA1209F | 0.1 mg | F | ||
| Mouse IgG1 Negative Control:Low Endotoxin | MCA1209EL | 0.5 mg | F | ||
| Mouse IgG1 Negative Control | MCA1209 | 0.1 mg | F | ||
| Mouse IgG1 Negative Control:RPE | MCA1209PE | 100 Tests | F |
Product Specific References
References for CD200 Receptor 1 antibody
-
Wright, G.J. et al. (2000) Lymphoid/neuronal cell surface OX2 glycoprotein recognizes a novel receptor on macrophages implicated in the control of their function.
Immunity. 13 (2): 233-42. -
Nathan, C. & Muller, W.A. (2001) Putting the brakes on innate immunity: a regulatory role for CD200?
Nat Immunol. 2 (1): 17-9. -
Dick, A.D. et al. (2001) Distribution of OX2 antigen and OX2 receptor within retina.
Invest Ophthalmol Vis Sci. 42 (1): 170-6. -
Banerjee, D. & Dick, A.D. (2004) Blocking CD200-CD200 receptor axis augments NOS-2 expression and aggravates experimental autoimmune uveoretinitis in Lewis rats.
Ocul Immunol Inflamm. 12 (2): 115-25. -
Meuth, S.G. et al. (2008) CNS inflammation and neuronal degeneration is aggravated by impaired CD200-CD200R-mediated macrophage silencing.
J Neuroimmunol. 194 (1-2): 62-9. -
Matsumoto, S. et al. (2015) CD200+ and CD200- macrophages accumulated in ischemic lesions of rat brain: the two populations cannot be classified as either M1 or M2 macrophages.
J Neuroimmunol. 282: 7-20. -
Lin, S.S. et al. (2012) Immune Characterization of Wild-Caught Rattus norvegicus Suggests Diversity of Immune Activity in Biome-Normal Environments
Journal of Evolutionary Medicine. 1: 1-16. -
Nicholls, S.M. et al. (2015) Local targeting of the CD200-CD200R axis does not promote corneal graft survival.
Exp Eye Res. 130: 1-8. -
Xie, X. et al. (2017) Monocytes, microglia and CD200-CD200R1 signaling are essential in the transmission of inflammation from the periphery to the central nervous system.
J Neurochem. Feb 6. [Epub ahead of print]
