CD200 Receptor 1 antibody | OX-102
Mouse anti Rat CD200 Receptor 1
- Product Type
- Monoclonal Antibody
- Clone
- OX-102
- Isotype
- IgG1
- Specificity
- CD200 Receptor 1
Antibody Quality and Performance Guarantee
| Mouse anti Rat CD200 Receptor 1 antibody, clone OX-102 recognizes the rat OX2 (CD200) receptor 1. This antigen is a heavily glycosylated ~60-100 kDa cell surface molecule expressed by cells of the myeloid lineage but not by T or B lymphocytes. Mouse anti Rat CD200 Receptor 1 antibody, clone OX-102 has been shown to block the interaction of OX2 receptor 1 with CD200 (Bushell et al. 2008). |
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Preparation
- MCA1959GA: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- MCA1959: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Membrane fraction of thioglycollate-elicited rat peripheral cells.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/100 | 1/200 |
| Immunohistology - Frozen | |
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| Immunoprecipitation | |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA1209 | F | 0.1 mg |
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Log in | ||
| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
Source Reference
-
Wright, G.J. et al. (2000) Lymphoid/neuronal cell surface OX2 glycoprotein recognizes a novel receptor on macrophages implicated in the control of their function.
Immunity. 13 (2): 233-42.
References for CD200 Receptor 1 antibody
-
Nathan, C. & Muller, W.A. (2001) Putting the brakes on innate immunity: a regulatory role for CD200?
Nat Immunol. 2 (1): 17-9. -
Dick, A.D. et al. (2001) Distribution of OX2 antigen and OX2 receptor within retina.
Invest Ophthalmol Vis Sci. 42 (1): 170-6. -
Banerjee, D. & Dick, A.D. (2004) Blocking CD200-CD200 receptor axis augments NOS-2 expression and aggravates experimental autoimmune uveoretinitis in Lewis rats.
Ocul Immunol Inflamm. 12 (2): 115-25. -
Meuth, S.G. et al. (2008) CNS inflammation and neuronal degeneration is aggravated by impaired CD200-CD200R-mediated macrophage silencing.
J Neuroimmunol. 194 (1-2): 62-9. -
Matsumoto, S. et al. (2015) CD200+ and CD200- macrophages accumulated in ischemic lesions of rat brain: the two populations cannot be classified as either M1 or M2 macrophages.
J Neuroimmunol. 282: 7-20. -
Nicholls, S.M. et al. (2015) Local targeting of the CD200-CD200R axis does not promote corneal graft survival.
Exp Eye Res. 130: 1-8. -
Xie, X. et al. (2017) Monocytes, microglia, and CD200-CD200R1 signaling are essential in the transmission of inflammation from the periphery to the central nervous system.
J Neurochem. 141 (2): 222-35. -
Chang, J.C. et al. (2019) Early Immune Response to Acute Gastric Fluid Aspiration in a Rat Model of Lung Transplantation.
Exp Clin Transplant. 17 (1): 84-92.
View The Latest Product References
-
Qian, H. et al. (2024) Activation of the CD200/CD200R1 axis improves cognitive impairment by enhancing hippocampal neurogenesis via suppression of M1 microglial polarization and neuroinflammation in hypoxic-ischemic neonatal rats.
Int Immunopharmacol. 128: 111532.
- Synonyms
- OX2 Receptor 1
- RRID
- AB_2074188
- UniProt
- Q9ES58
- Entrez Gene
- Cd200r1
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
MCA1959GA
150609
MCA1959
160242
1804
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