F4/80 Antibody References

F4/80 clone CI:A3-1 the original and the best with pedigree

The anti F4/80 antibody is derived from Clone CI:A3-1. This clone is the original anti F4/80 antigen with an established heritage going back to 1981 when research data was first published. Since then there have been hundreds of citations mentioning the use of Bio-Rad’s F4/80 antibody.

Listed are a selection of references, organized chronologically and which provide more information about detailed methods, uses and advantages of our products.

Further references can be found on CiteAb, where there are over 900 F4/80 clone CI:A3-1 citations.


  • Austyn JM and Gordon S (1981). F4/80, a monoclonal antibody directed specifically against the mouse macrophage. 
    Eur. J. Immunol. 11: 805-815.
  • Hume DA et al. (1984). The Mononuclear Phagocyte System of the Mouse defined by Immunohistochemical Localisation of Antigen F4/80: Macrophages Associated with Epithelia.
    The Anatomical Record 210: 503-512.
  • Lee SH et al. (1985) Quantitative analysis of total macrophage content in adult mouse tissues. Immunochemical studies with monoclonal antibody F4/80.
    J. Exp. Med. 161: 475-489.
  • Gordon S et al. (1992). Antigen markers of macrophage differentiation in murine tissues.
    Curr. Top. Micro. Immunol. 181: 1-37.
  • Chan RJ et al. (2005). Human somatic PTPN11 mutations induce hematopoietic cell hypersensitivity to granulocyte-macrophage colony stimulating factor.
    Blood. 105: 3737-3742.
  • Lin HH et al. (2005). The macrophage F4/80 receptor is required for the induction of antigen-specific efferent regulatory T cells in peripheral tolerance.
    J. Exp. Med. 201: 1615–1625.
  • Pizza FX et al. (2005). Neutrophils contribute to muscle injury and impair its resolution after lengthening contractions in mice.
    J. Physiol. 562:899-913.
  • Cunningham O et al. (2009). Microglia and the urokinase plasminogen activator receptor/uPA system ininnate brain inflammation.
    Glia. 57: 1802-14.
  • Hemmi H et al. (2009). A new triggering receptor expressed on myeloid cells (Trem) family member,Trem-like 4, binds to dead cells and is a DNAX activation protein 12-linkedmarker for subsets of mouse macrophages and dendritic cells.
    J Immunol. 182:1278-86.
  • Choi KM et al. (2010). CD206-positive M2 macrophages that express heme oxygenase-1 protect against diabetic gastroparesis in mice.
    Gastroenterology. 138: 2399-409,
  • Miao EA et al. (2011). Caspase-1-induced pyroptosis is an innate immune effector mechanism against intracellular bacteria.
    Nat Immunol. 11: 1136-42.
  • Seitz O et al. (2010). Wound Healing in Mice with High-Fat Diet- or ob Gene-Induced Diabetes-Obesity Syndromes: A Comparative Study.
    Exp Diabetes Res. 2010: 476969.
  • Tarallo V et al. (2011). The biflavonoid amentoflavone inhibits neovascularization preventing the activity of proangiogenic vascular endothelial growth factors.
    J Biol Chem. 286: 19641-51.
  • Wang X et al. (2011). Activation of the cholinergic antiinflammatory pathway ameliorates obesity-induced inflammation and insulin resistance.
    Endocrinology. 152: 836-46.
  • Akbarshahi, H. et al. (2012). Enrichment of Murine CD68(+)CCR2(+) and CD68(+)CD206(+) Lung Macrophages in Acute Pancreatitis-Associated Acute Lung Injury.
    PLoS One. 7: e42654.
  • Banda NK et al. (2012). Role of C3a receptors, C5a receptors, and complement protein C6 deficiency in collagen antibody-induced arthritis in mice.
    J Immunol. 188: 1469-78.
  • Bonde AK et al. (2012). Intratumoral macrophages contribute to epithelial-mesenchymal transition in solid tumors.
    BMC Cancer. 12: 35.
  • Gornicka A et al. (2012). Adipocyte hypertrophy is associated with lysosomal permeability both in vivo and in vitro: role in adipose tissue inflammation.
    Am J Physiol Endocrinol Metab. 303: E597-606.
  • Rivollier A et al. (2012). Inflammation switches the differentiation program of Ly6Chi monocytes from antiinflammatory macrophages to inflammatory dendritic cells in the colon.
    J Exp Med. 209: 139-55.
  • Tamaki, S. et al. (2013). Interleukin-16 promotes cardiac fibrosis and myocardial stiffening in heart failure with preserved ejection fraction.
    PLoS One. 8: e68893.
  • Uaesoontrachoon K et al. (2013). Osteopontin deficiency delays inflammatory infiltration and the onset of muscle regeneration in a mouse model of muscle injury.
    Dis Model Mech. 6: 197-205.
  • Kihira Y et al. (2014). Deletion of hypoxia-inducible factor-1a in adipocytes enhances glucagon-like Peptide-1 secretion and reduces adipose tissue inflammation.
    PLoS One. 9(4):e93856.

Other related macrophage markers by species reactivity

In addition to F4/80, Bio-Rad also offers a wide range of other macrophage markers such as:

Anti-Mouse Antibodies

Anti-Human Antibodies

Anti-Rat Antibodies