CD36, also known SCARB3, GPIV, GPIIIB, FAT, and PAS IV, is a member of the class B scavenger receptor family. It is expressed on a broad range of blood and immune cell types including: platelets, monocytes, macrophages, erythrocytes, differentiated adipocytes, and mammary epithelial cells. CD36 has diverse functions, playing a role in innate immunity, thrombosis, atherosclerosis, cellular adhesion, and lipid transport.
In its role as a receptor protein, CD36 binds a variety of ligands including: thrombospondin, collagen, oxidized low-density and native lipoproteins, oxidized phospholipids, and long-chain fatty acids. CD36 plays an important part in malaria infections, directly mediating cytoadhesion of erythrocytes that have been parasitized by Plasmodium falciparum through interaction with PfEMP1, working in conjunction with TLR2 – TLR6.
Bio-Rad manufactures several monoclonal antibodies directed against human and mouse CD36, as well as a rabbit anti human polyclonal that cross-reacts with a wide range of species.
Anti -mouse CD36 (MCA2748, clone MF3) can be purchased from Bio-Rad in a range of sizes and formats. It is available conjugated to FITC or ALEXA FLUOR® 488 for flow cytometry or immunofluorescence, and purified for use in immunoprecipitations and Western blotting. Since clone MF3 has been shown to inhibit IL-4 induced macrophage fusions, we also offer a Low Endotoxin version for use in functional studies.
Anti-human CD36 (MCA722, clone SMØ) is available Purified, Azide Free and conjugated to ALEXA FLUOR® 647 and FITC. The fluorescent versions are guaranteed for flow cytometry, while the purified versions are suitable for staining frozen sections. Clone SMØ is available in an Azide Free format which has been shown to work in blocking studies against oxidized low-density lipoproteins.
To learn more about Toll-like receptors and their role in innate immunity visit our resource page.