F4/80 Antibody | Cl:A3-1

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F4/80 Antibody | Cl:A3-1 gallery image 1

Dot Blot showing FSC/SSC gated mouse peritoneal macrophages dual stained with F4/80 (MCA497A647) at a 1/5 dilution and CD11b (MCA74F) at a 1/5 dilution. Isotype control pair in red. Fc receptors were blocked by Mouse Seroblock (BUF041B)

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Dot Blot showing FSC/SSC gated mouse peritoneal macrophages dual stained with F4/80 (MCA497F) at a 1/5 dilution and CD11b (MCA74A647) at a 1/5 dilution. Isotype control pair in red. Fc receptors were blocked by Mouse Seroblock (BUF041B)

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Dot Blot showing FSC/SSC gated mouse peritoneal macrophages dual stained with F4/80 (MCA497APC) at a 1/5 dilution and CD169 (MCA884F) at a neat dilution. Isotype control pair in red. Fc receptors were blocked by Mouse Seroblock (BUF041B)

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Mouse spleen cryosection stained with Rat anti Mouse F4/80 antibody, clone Cl:A3-1 (MCA497G) at a 1/100 dilution followed by Goat anti Rat IgG:HRP (STAR72) at a 1/50 dilution

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Figure A. Mouse spleen stained with Rat anti Mouse F4/80 antibody, clone Cl:A3-1 (MCA497G) at a dilution of 1/100 followed by Goat anti Rat IgG:HRP (STAR72) at 1/50 dilution. Figure B. Rat anti Mouse F4/80 (MCA497) preincubated with 2 molar excess of Human anti Idiotypic MCA497 (HCA154) followed by Goat anti Rat IgG:HRP (STAR72)

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Rat anti Mouse F4/80 antibody, clone A3-1 used for the identification of macrophages in injured soleus muscle by immunohistochemistry of formalin fixed, paraffin embedded tissue sections.
Image caption:
PCNA and F4/80 immunohistochemical expression after muscular injury. WT: wild type, KO: knockout. (A) Immunohistochemistry of PCNA. (Scale bar = 50μm), (B) Quantitative analysis of PCNA-positive cells, (C) Immunohistochemistry of F4/80. (Scale bar = 50μm), (D) Quantitative analysis of F4/80-positive cells. (B and D) Both PCNA and F4/80 expression in p21KO mice at 3 days after injury were the highest (p < 0.05).

From: Chinzei N, Hayashi S, Ueha T, Fujishiro T, Kanzaki N, Hashimoto S, et al. (2015) P21 Deficiency Delays Regeneration of Skeletal Muscular Tissue.
PLoS ONE 10(5): e0125765.

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Rat anti Mouse F4/80 antibody, clone A3-1 used for the detection of infiltrating macrophages in tumor tissue by immunofluorescence.
Image caption:
Presence of inflammatory cells in tumor tissue. Macrophage and neutrophil infiltration are unaffected by loss of serglycin in RIP1-Tag2 tumors. Tumor sections from 15w RTposSGwt and RTposSGko mice were immunostained from the neutrophil and macrophage markers Gr-1 (a) and F4/80 (b) respectively. For Gr-1, the number of positive cells/mm2 was calculated (c) and there was no difference in infiltration of neutrophils between the two groups. For F4/80, the area of positive staining was measured (d) and although there was a slight trend to decreased macrophage infiltration in serglycin deficient animals, this was not significant. Each data point in represents an individual animal. Statistical analysis was performed using a two-tailed Mann-Whitney test. Error bars represent mean ± SEM.

From: Hamilton A, Basic V, Andersson S, Abrink M, Ringvall M (2015) Loss of Serglycin Promotes Primary Tumor Growth and Vessel Functionality in the RIP1-Tag2 Mouse Model for Spontaneous Insulinoma Formation.
PLoS ONE 10(5): e0126688.

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Rat anti Mouse F4/80 antibody used for the detection of macrophage processes in mouse kidney tissue sections by immunofluorescence.
Image caption:
Dual immunofluorescence staining showing the association of F4/80+ cells with deposited C3. Shown is a representative immunofluorescence micrograph of a Crry-/-C3-/- kidney 7 days after transplantation into a wildtype recipient. Considerable deposition of C3 (green) is evident in the basolateral aspects of tubules, along with closely approximated F4/80+ cellular processes (red).

From: Chaves LD, Bao L, Wang Y, Chang A, Haas M, Quigg RJ (2014) Loss of CD11b Exacerbates Murine Complement-Mediated Tubulointerstitial Nephritis.
PLoS ONE 9(3): e92051.

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FITC-conjugated Rat anti Mouse F4/80 antibody, clone A3-1 used for the detection of F4/80 expressing cells in peritoneal cell exudates by flow cytometry.
Image caption:
Identification of resident peritoneal Mø subsets. PC from C57BL/6 were harvested and stained with fluorochrome-labeled antibodies directed against F4/80, CD19, CD11c and IAb for flow cytometry analysis. (A) Doublet cells were excluded according to forward scatter profiles (FSC-A and FSC-H). Subsequently, (B) CD19 high cells and (C) CD11c high cells were also excluded, and (D) F4/80+ cells were selected. (E) F4/80 and IAb expression defined three populations: LPM (F4/80highIAb-neg), SPM (F4/80lowIAb-high) and granulocytes (F4/80lowIAb-neg). These three subpopulations were purified by cell sorting on a FACS Vantage, and their morphology was evaluated ex vivo from cytospin slides (F), or after in vitro culture in chamber slides for 12 h (G). Slides were stained with hematoxylin and eosin (H&E) and analyzed by optical microscopy (40×).

From: Cassado AdA, de Albuquerque JAT, Sardinha LR, Buzzo CdL, Faustino L, Nascimento R, et al. (2011) Cellular Renewal and Improvement of Local Cell Effector Activity in Peritoneal Cavity in Response to Infectious Stimuli.
PLoS ONE 6(7): e22141.

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FITC-conjugated Rat anti Mouse F4/80 antibody, clone A3-1 used for the detection of F4/80 expressing cells in peritoneal cell exudates by flow cytometry.
Image caption:
Zymosan and T. cruzi injection alters the Mø compartment of PerC. C57BL/6 mice were injected i.p. with zymosan (1 mg/mouse) or T. cruzi (106 parasites/mouse) and at 30 min (A) or 48 h (B) after stimulation, PC from naive and injected mice were harvested and stained as described in M&M. Sequential gates were made as shown in Fig. S1. Plots show the frequencies of each subpopulation. (C) F4/80lowMHCIIint cells present within PerC 48 h after injections were evaluated according the expression of Ly6C. Gray lines represent FMO [23], [25], the black lines show F4/80lowMHCIIint cells from zymosan- (hairline) or T. cruzi-(bold line)-exposed PerC. (D) Total numbers of SPM, LPM and monocytes 48 h after zymosan or T. cruzi exposure (within Mø gate) are shown in panel. Data are representative of more than 3 independent experiments.

From: Cassado AdA, de Albuquerque JAT, Sardinha LR, Buzzo CdL, Faustino L, Nascimento R, et al. (2011) Cellular Renewal and Improvement of Local Cell Effector Activity in Peritoneal Cavity in Response to Infectious Stimuli.
PLoS ONE 6(7): e22141.

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FITC-conjugated Rat anti Mouse F4/80 antibody, clone A3-1 used for the detection of F4/80 expressing cells in peritoneal cell exudates by flow cytometry.
Image caption:
SPM are more responsive than LPM and monocytes to infectious stimuli. C57BL/6 mice were injected i.p. with zymosan (1 mg/mouse) or T. cruzi (106 parasites/mouse) and PCs were harvested 30 min or 48 h after stimulation. (A) SPM and LPM from C57BL/6 zymosan-exposed mice were FACS-sorted 30 min after injection and the presence of internalized zymosan particles was observed by optical microscopy. Slides were made with 105 cells from purified Mø subsets, stained with H&E and analyzed by optical microscopy (40×). (B) Numbers represent the mean ±SD of internalized zymosan particles per cell in each Mø subset. *** p<0.001 when compared to the LPM group. (C) PC from control or 48 h-exposed mice were cultured for 6 h in the presence of brefeldin A with or without LPS (1 μg/ml) plus rIFN-γ (5 ng/ml). Titles above plots indicate in vivo - in vitro stimulations. Values inside gates represent the frequencies of IL-12-producing cells in each subpopulation. Data are representative of more than 3 independent experiments.

From: Cassado AdA, de Albuquerque JAT, Sardinha LR, Buzzo CdL, Faustino L, Nascimento R, et al. (2011) Cellular Renewal and Improvement of Local Cell Effector Activity in Peritoneal Cavity in Response to Infectious Stimuli.
PLoS ONE 6(7): e22141.

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Rat anti Mouse F4/80 antibody used for the detection of macrophages in mouse lung and pancreas by immunohistochemistery on formalin fixed, paraffin tissue sections.
Image caption:
Changes in pancreatic and lung F4/80+ macrophage number during acute pancreatitis. Representative photomicrographs of pancreas (A) and lung (C) sections stained for F4/80 as indicator of macrophage infiltration (brown) 24 h after pancreatitis induction (original magnification, ×20). Quantification of F4/80+ cells showed a significantly increased enrichment in the pancreas from 9 h following pancreatitis compared to sham controls (B). No significant difference was observed in the number of lung F4/80+ cells between pancreatitis and sham control groups (D). Bars show mean ± SEM, n = 10 per group. **P<0.01, ***P<0.001, by two-tailed Student t-test.

From: Akbarshahi H, Menzel M, Posaric Bauden M, Rosendahl A, Andersson R (2012) Enrichment of Murine CD68+CCR2+ and CD68+CD206+ Lung Macrophages in Acute Pancreatitis-Associated Acute Lung Injury.
PLoS ONE 7(10): e42654.

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Rat anti Mouse F4/80 antibody, clone A3-1 used for the evaluation of F4/80 expression on isolated Kuppfer and peritoneal cells by flow cytometry.
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CLEC4F is co-expressed with F4/80 on liver Kupffer cells.
(A) CLEC4F and F4/80 immunohistochemistry of parafilm-embedded liver sections from wild-type and Clec4f-/- mice. (B) Double immunofluorescence of CLEC4F and F4/80 in wild-type livers was performed. Nuclei were counterstained with Hoechst 33342. Signals were determined by confocal microscope (magnification 10×63). (C) Coexpression of CLEC4F and F4/80 on Kupffer cells, but not peritoneal macrophages. Cells were double stained with Alexa Fluor 647-conjugated anti-F4/80 and PE-conjugated anti-CLEC4F mAb. Alexa Fluor 647-conjugated rat IgG2b and PE-conjugated mIgG1 were used as isotype controls.
From: Yang C-Y, Chen J-B, Tsai T-F, Tsai Y-C, Tsai C-Y, Liang P-H, et al. (2013) CLEC4F Is an Inducible C-Type Lectin in F4/80-Positive Cells and Is Involved in Alpha-Galactosylceramide Presentation in Liver.
PLoS ONE 8(6): e65070.

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Rat anti Mouse F4/80 antibody, clone A3-1 used for the evaluation of F4/80 expression on thymic myeloid cells by flow cytometry.
Image caption:
Heterogeneous phenotype of thymic resident myeloid cells. (A) Thymic stromal cells were enriched by collagenase/dispase digestion followed by percoll density gradient centrifugation. Cells were then stained with anti-CD11b and anti-F4/80 and analyzed by FACS. Red circles mark the three different cell populations discussed in the text. In this experiment, populations #1, #2 and #3 represented 0.02%, 0.43% and 0.61% of the analyzed cells, respectively. While the absolute cell number varied as a result of the preparation, the percentage of each cell type relative to each other was consistent over many experiments. (B) Size and surface marker expression (blue lines) of each population was determined by FACS. Populations were identified as in (A) and stained with the antibodies as indicated. Isotype controls (red lines) were used to correct differences in the autofluorescence of each cell population. (C) Individual cells from populations #2 and #3 were sorted onto glass slides followed by staining with H&E. Data are representative of more than ten experiments (a and b) and three experiments (c).

From: Kim H-J, Alonzo ES, Dorothee G, Pollard JW, Sant'Angelo DB (2010) Selective Depletion of Eosinophils or Neutrophils in Mice Impacts the Efficiency of Apoptotic Cell Clearance in the Thymus.
PLoS ONE 5(7): e11439.

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Rat anti Mouse F4/80 antibody, clone A3-1 used for the evaluation of F4/80 expression on thymic myeloid cells following irradiation by immunofluorescence.
Image caption:
Time course analysis of F4/80 positive cells following irradiation.
Thymuses from wild type B6 mice were harvested and frozen post-irradiation at the indicated times (1 hour through 24 hours). Sections were stained with anti-F4/80 (red), which identifies macrophages and eosinophils. Panel labeled "control" was a section stained with secondary antibody only. Panel labeled “w/o irr.” was a section from a nonirradiated mouse. Original magnification was 200X.

From: Kim H-J, Alonzo ES, Dorothee G, Pollard JW, Sant'Angelo DB (2010) Selective Depletion of Eosinophils or Neutrophils in Mice Impacts the Efficiency of Apoptotic Cell Clearance in the Thymus.
PLoS ONE 5(7): e11439.

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Rat anti Mouse F4/80 antibody, clone A3-1 used for the evaluation of F4/80 expression on thymic myeloid cells following irradiation by flow cytometry.
Image caption:
Influx of innate cells rapidly declines twenty-four hours after irradiation. Thymuses were harvested from mice at the indicated times after irradiation. Stromal cells were enriched as described and stained with anti-CD11b, -F4/80, SiglecF and -Ly-6G. Numbers in the plots are the percentage of cells within the electronic gate. N = 6 and the data show the results from two independent experiments. The mean is shown by a horizontal bar and statistics were calculated with 2-tailed, nonpaired Mann-Whitney test. * = <0.01; ** = <0.001; *** = <0.0001..

From: Kim H-J, Alonzo ES, Dorothee G, Pollard JW, Sant'Angelo DB (2010) Selective Depletion of Eosinophils or Neutrophils in Mice Impacts the Efficiency of Apoptotic Cell Clearance in the Thymus.
PLoS ONE 5(7): e11439.

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Rat anti Mouse F4/80 antibody, clone A3-1 used for the evaluation of F4/80 expression on spleen and bone marrow cells by flow cytometry.
Image caption:
FACS analyses of the innate cells in the spleen and bone marrow from WT and Csf op/op mice. Cells from the spleen and bone marrow from WT and Csfop/op mice were stained for CD11b and F4/80. Eosinophils and neutrophils were identified by staining cells for CD11b and SiglecF or CD11b and Gr-1, respectively. Percent of cells in each area is indicated in the Figure.

From: Kim H-J, Alonzo ES, Dorothee G, Pollard JW, Sant'Angelo DB (2010) Selective Depletion of Eosinophils or Neutrophils in Mice Impacts the Efficiency of Apoptotic Cell Clearance in the Thymus.
PLoS ONE 5(7): e11439.

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Rat anti Mouse F4/80 antibody, clone A3-1 used for the evaluation of F4/80 expression on thymic cells by flow cytometry.
Image caption:
Impaired clearance of apoptotic thymocytes in Csf1op/op mice. (A) Thymic stromal cell subpopulations from WT (Csf1op/+) and osteopetrosis (Csf1op/op) mice were stained with the indicated antibodies and analyzed by FACS. Numbers indicate the percentage of cells within the circles. The red circle marks population #1, which is not present in Csf1op/op mice. (B) Comparison of thymic stromal cell subpopulations between WT (Csf1op/+) and osteopetrosis (Csf1op/op) mice 16 hours after irradiation. Thymic stromal cells were enriched and stained for CD11b and F4/80. (C) Representative images showing the frequency of apoptotic cells in WT (Csf1op/+) and Csf1op/op mice in nonirradiated mice in mice 24 hrs receiving 1Gy irradiation. Apoptotic cells were detected by TUNEL (green) and their cortical localization was visualized by counterstaining of thymic sections with an antibody against DEC205 (red). Original magnification was 100X. Sections are representative of multiple samples from more than ten experiments.

From: Kim H-J, Alonzo ES, Dorothee G, Pollard JW, Sant'Angelo DB (2010) Selective Depletion of Eosinophils or Neutrophils in Mice Impacts the Efficiency of Apoptotic Cell Clearance in the Thymus.
PLoS ONE 5(7): e11439.

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Phycoerythrin conjugated Rat anti Mouse F4/80 antibody, clone A3-1 used for the identification of inflammatory macrophages isolated from ischemic muscle by flow cytometry.
Image caption:
Thrombospondin-1-/- mice exhibit a less pro-inflammatory macrophage activation state in response to ischemia. (A) FACS analyses of CD45+ cells isolated from ischemic muscles at d4, stained for F4/80 and Ly-6C expression, wt, left panel; tsp-1-/-, right panel; n = 5 mice per group. (B) Quantification of F4/80(lo)Ly-6C(hi), F4/80(hi)Ly-6C(hi) and F4/80(hi)Ly-6C(lo) macrophage proportions in CD45+ cells isolated from ischemic muscles at d4 in both genotypes (n = 5 mice per group in five independent experiments). * = p<0,05 vs. wt. (C) Quantitative RT-PCR analyses of IL-1β, TNF-α, IL-10, PPAR-γ, TGF-β, iNOS and Arg1 expression in F4/80(hi) vs. F4/80(lo)Ly-6C(hi) cells isolated from ischemic muscles at d4 in both genotypes.

From: Bréchot N, Gomez E, Bignon M, Khallou-Laschet J, Dussiot M, Cazes A, et al. (2008) Modulation of Macrophage Activation State Protects Tissue from Necrosis during Critical Limb Ischemia in Thrombospondin-1-Deficient Mice. PLoS ONE 3(12): e3950.

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Phycoerythrin conjugated Rat anti Mouse F4/80 antibody, clone A3-1 used for the identification of inflammatory macrophages isolated from ischemic muscle by flow cytometry.
Image caption:
Kinetic analysis of intra-tissular macrophage activation state during ischemia. (A) Mononuclear cells were isolated from ischemic muscles of C57Bl6 mice (Charles-River) using centrifugation over Ficoll, and analyzed by FACS for F4/80 and Ly-6C expression (n = 3 per time point). (B) SSC/FSC characteristics and CD11b expression of Ficoll-isolated F4/80(lo) Ly-6C(hi) cells at d4, showing an homogeneous SSC(lo) CD11b(hi) macrophage population.

From: Bréchot N, Gomez E, Bignon M, Khallou-Laschet J, Dussiot M, Cazes A, et al. (2008) Modulation of Macrophage Activation State Protects Tissue from Necrosis during Critical Limb Ischemia in Thrombospondin-1-Deficient Mice. PLoS ONE 3(12): e3950.

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Phycoerythrin conjugated Rat anti Mouse F4/80 antibody, clone A3-1 used for the identification of inflammatory macrophages isolated from ischemic muscle by flow cytometry.
Image caption:
Thrombospondin-1-/- mice exhibit a less pro-inflammatory macrophage activation state in response to ischemia. Additional experiments. (A) SSC/FSC characteristics and CD11b expression of CD45+ F4/80(lo) Ly-6C(hi) cells isolated from ischemic muscles at d4, in both genotypes, showing an homogeneous SSC(lo)CD11b(hi) macrophage population. (B) FACS analyses of CD45+ cells isolated from ischemic muscles at d4, stained for F4/80 and Ly-6C expression. Additional examples of two independent experiments are shown (n = 5 mice). (C) Upper panel, representative FACS analyses of Ficoll-isolated mononuclear cells from ischemic muscles of one mouse from both genotypes at d4, stained for F4/80 and Ly-6C. Lower panel, quantification of F4/80(lo)Ly-6C(hi), F4/80(hi)Ly-6C(hi) and F4/80(hi)Ly-6C(lo) macrophage proportions in both genotypes (n = 5).

From: Bréchot N, Gomez E, Bignon M, Khallou-Laschet J, Dussiot M, Cazes A, et al. (2008) Modulation of Macrophage Activation State Protects Tissue from Necrosis during Critical Limb Ischemia in Thrombospondin-1-Deficient Mice. PLoS ONE 3(12): e3950.

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Rat anti Mouse F4/80 antibody, clone A3-1 used for the identification of macrophages in mouse spleen using immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Depletion of F4/80-expressing cells in spleens derived from clodrolip-treated Ptchflox/floxERT2+/- mice. Immunohistochemical analysis using an anti-F4/80 antibody of paraffin-embedded spleens of Ptchflox/floxERT2+/- mice treated with empty liposomes or clodrolip.

From: König S, Nitzki F, Uhmann A, Dittmann K, Theiss-Suennemann J, Herrmann M, et al. (2014) Depletion of Cutaneous Macrophages and Dendritic Cells Promotes Growth of Basal Cell Carcinoma in Mice.
PLoS ONE 9(4): e93555.

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Rat anti Mouse F4/80 antibody, clone A3-1 used for the identification of infiltrating macrophages in tumor tissue by immunofluorescence.
Image caption:
CSF1 has no effect on tumor growth but increases percent tumor TEMs and augments angiogenesis. (A) After two weeks of treatment, tumors were removed, homogenized and immunostained with antibodies specific for F4/80 and Tie2 to identify total F4/80+ cells and F4/80+/Tie2+ cells (Tie2-expressing macrophages, TEMs). While there was a marked increase in total F4/80+ macrophages with CSF1 treatment, the percent of F4/80+/Tie2+ TEMs was significantly increased in response to CSF1 suggesting a regulatory role for CSF1 in expanding the TEM population. N = 5 mice per group and results represent the mean ± SEM of total F4/80+ and F4/80+/Tie2+ TEMs within the tumors. (B, top and bottom left) PyMT tumors without CSF1 treatment and (top and bottom right) with CSF1 treatment immunostained with CD31 for blood vessels, F4/80 for macrophages, Tie2 for F4/80+/Tie2+ TEMS, and DAPI. Confocal images (using 60× objective (top) and with 3× zoom (bottom) suggest an increase in both F4/80 macrophages and F4/80+/Tie2+ TEMS in the CSF1-treated tumors. Multiply overlap indicates those areas where F4/80 and Tie2 positivity overlap. Individual stains are in Supplementary Figure 3. (C, top) Orthotopically implanted PyMT mammary tumors in wild type C57Bl/6 female mice were allowed to become palpable then intraperitoneally treated with PBS (PBS), CSF1 (100 ng in 100 μls) (CSF1), a neutralizing antibody for the CSF1R (50 mg/kg) 4 hours prior to CSF1 treatment (100 ng in 100 μls) (CSF1R NAb+CSF1), the CSF1R antibody alone (CSF1R NAb), an isotype antibody (50 mg/kg) 4 hours prior to CSF1 (100 ng in 100 μls) treatment (CSF1+IgG), or the isotype antibody alone (IgG) three times per week for two additional weeks. The tumors were immunostained with a CD31-Alexa Flour 546 antibody to recognize endothelial cells that comprise blood vessels. Qualitatively, CSF1 treatment increased the percent of CD31-postitive pixels per high powered field compared to PBS treated tumors, while the neutralizing antibody to CSF1R suppressed the CSF1 effect on angiogenesis. (B, bottom) Quantitatively, the percent of CD31+ pixels per high powered field were quantified as blood vessels (angiogenesis) using Adobe Photoshop histogram analysis. CSF1 treatment significantly increased CD31-positive pixels (angiogenesis) compared to PBS. The neutralizing antibody for CSF1R significantly reduced the ability of CSF1 to up-regulate angiogenesis. N = 5 mice per group and results represent the mean ± SEM of percent CD31-positive pixels per high powered field (HPF).

From: Forget MA, Voorhees JL, Cole SL, Dakhlallah D, Patterson IL, Gross AC, et al. (2014) Macrophage Colony-Stimulating Factor Augments Tie2-Expressing Monocyte Differentiation, Angiogenic Function, and Recruitment in a Mouse Model of Breast Cancer. PLoS ONE 9(6): e98623.

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Rat anti Mouse F4/80 antibody, clone A3-1 used for the identification of infiltrating macrophages in the spleen of Entamoeba histolytica infected mice by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Histological and immunohistochemical characterization of cell infiltrates during ALA. (A) H&E staining of mouse liver abscesses (indicated by the square in the top row of images) at the indicated times post-infection with E. histolytica trophozoites. (B) PAS staining shows E. histolytica trophozoites (arrowheads) within the abscess. (C and D) Tissue sections were stained with anti-7/4 (C) and anti-F4/80 (D) antibodies followed by HRP-conjugated secondary antibody to detect neutrophils and macrophages, respectively (brown).

From: Helk E, Bernin H, Ernst T, Ittrich H, Jacobs T, Heeren J, et al. (2013) TNFa-Mediated Liver Destruction by Kupffer Cells and Ly6Chi Monocytes during Entamoeba histolytica Infection.
PLoS Pathog 9(1): e1003096.

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Rat anti Mouse F4/80 antibody, clone A3-1 used for immunohistochemistry on mouse brain sections
Image caption:
Representative photographs of cortical area in (a) CD40 def. and (b) wild-type mice stained with F4/80 antibody at 8 to 10 weeks old (each bar represents 0.1 mm).

From: Laporte et al.
Journal of Neuroinflammation 2006 3:3.

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Rat anti Mouse F4/80 antibody, clone A3-1 used for the detection of microglia in mouse brain by immunofluorescence
Image caption:
Post-injury 7,8-dihydroxyflavone treatment increased brain BDNF levels and promoted CREB activation. (A) Bar graphs demonstrating brain derived neurotrophic factor (BDNF) protein concentrations measured by enzyme-linked immunosorbent assay (ELISA) in sham-injured, vehicle-treated, and 20 mg/kg 7,8-dihydroxyflavone (DHF 20)-treated mice at 4 days post-TBI. DHF 20 significantly increased BDNF protein levels in the ipsilateral hemisphere at 4 days post-injury. (B) Bar graphs demonstrating BDNF mRNA expression measured by real–time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) at 1 and 4 days post-injury. DHF 20 significantly increased BDNF mRNA levels in the ipsilateral hemisphere at 4 days post-injury. (C) Western blot analysis of the phospho-CREB level in the ipsilateral hemisphere of sham-injured, vehicle-treated, and DHF 20-treated mice at 1 h, 1 day, and 4 days post-injury. DHF 20 enhanced CREB phosphorylation at 4 days post-injury. (D) Identification of phospho-CREB-positive cells 4 day post-injury in the peri-contusional margin by double immunofluorescence staining. Phospho-CREB is shown in red, and NeuN (neurons), GFAP (astrocytes), or F4/80 (microglia) is shown in green. Co-localization of phospho-CREB with NeuN is shown by yellow labeling. Sections were stained with DAPI (blue) to show all nuclei. Values are mean ± SEM; *P<0.05, **P<0.01, and ***P<0.001 versus sham group, #P<0.05, ##P<0.01, and ###P<0.001 versus vehicle group (n = 6–9 mice/group for BDNF protein and 7–9 mice/group for phospho-CREB, one-way ANOVA; n = 6–8 mice/group for BDNF mRNA, one-way ANOVA or Student's t-test). The scale bar is 50 &mum. Ipsi: ipsilateral cortex; Contra: contralateral cortex.

From: Wu C-H, Hung T-H, Chen C-C, Ke C-H, Lee C-Y, et al. (2014) Post-Injury Treatment with 7,8-Dihydroxyflavone, a TrkB Receptor Agonist, Protects against Experimental Traumatic Brain Injury via PI3K/Akt Signaling.
PLoS ONE 9(11): e113397.

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Rat anti Mouse F4/80 antibody, clone A3-1 used for the detection of infiltrating macrophages in mouse kidney by immunohistochemistry on paraffin embedded material
Image caption:
Reduction in cellular infiltration and inflammatory markers in UUO kidneys exposed to telmisartan or PXS64. Untreated UUO kidneys showed increased F4/80, CD68 and CD45 positively stained cells as compared to the sham operated control animals. PXS64 significantly reduced F4/80 and CD45 positive stained cells (Fig. 6C and E) with a trend to a reduction in CD68 cells, although this was not statistically significant (Fig. 6D). Telmisartan treated kidneys showed a reduction in F4/80 positive cells but no difference in CD45 or CD68 stained cells, suggesting a differential action of PXS64 and telmisartan in modifying cellular infiltration. Results are presented as mean showed (n = 8, *P<0.05 vs. UUO, ** P<0.01 vs. UUO). Magnification x 400.

From: Zhang J, Wong MG, Wong M, Gross S, Chen J, et al. (2015)
A Cationic-Independent Mannose 6-Phosphate Receptor Inhibitor (PXS64) Ameliorates Kidney Fibrosis by Inhibiting Activation of Transforming Growth Factor-β1.
PLoS ONE 10(2): e0116888.

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F4/80 Antibody | Cl:A3-1 gallery image 28

Published customer image:
Rat anti Mouse F4/80 antibody, clone A3-1 used for the identification of macrophages in formalin fixed, paraffin embedded murine skin tissue sections by immunohistochemistry.
Image caption:
L-tryptophan administration reverses stress-induced alterations on inflammatory response and lipid peroxidation. Mice were daily submitted to restraint stress and treated with L-tryptophan (Trp) or vehicle until euthanasia. Two days after the beginning of the stress protocol, two full-thickness excisional lesions (8-mm diameter) were made on the dorsal skin. Five days after wounding, lesions were collected and the number of myeloperoxidase (MPO)-positive neutrophils (B), F4/80-positive macrophages (C) and CD-3 positive T lymphocytes (D) were counted. Representative images (A) of staining for neutrophils, macrophages and T cells on wound area were presented. In addition, the protein levels of monocyte chemotactic protein-1 (MCP-1) (12 kDa) (E) were estimated in wound lysate by western-blotting. Representative images for immunoblotting for MCP-1 (F) were presented. The β-actin (42 kDa) was used as a loading control protein. Vertical black lines show non-adjacent bands from the same blot. To evaluate the oxidative damage in lipids, the levels of lipid peroxides (G) were measured in wound lysate using colorimetric assay. Data (n = 5) are presented as mean ± SEM. #p<0.05 vs. stressed (ST) group. Bar = 50 μm.

From: Bandeira LG, Bortolot BS, Cecatto MJ, Monte-Alto-Costa A, Romana-Souza B (2015) Exogenous Tryptophan Promotes Cutaneous Wound Healing of Chronically Stressed Mice through Inhibition of TNF-α and IDO Activation.
PLoS ONE 10(6): e0128439.

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F4/80 Antibody | Cl:A3-1 gallery image 29

Published customer image:
Rat anti Mouse F4/80 antibody, clone Cl:A3-1 used for the identification of macrophages in a murine colitis model by immunohistochemistry on formalin fixed paraffin embedded tissue sections.
Image caption:
Nur77-/- mice show more macrophage and T-cell influx in TNBS-induced colitis. The numbers of T-cells in the mucosa were counted and the influx was calculated by taking the ratio of Healthy / TNBS. Representative photomicrographs of sections stained for T-cells (anti-CD3) are shown (A). mRNA was extracted from colon and qPCR was performed for FoxP3 (B). cDNA levels were normalized for 36B4 housekeeping gene expression. Macrophage surface was quantified in the mucosa and the number of macrophages was counted in the muscle. The ratio of Healthy / TNBS was calculated. Representative photomicrographs of sections stained for macrophages (anti-F4/80) are shown (C). The numbers of Ki67 positive cells were counted per crypt and representative photomicrographs are shown (D). Colon lysates were prepared to determine IL-6, TNFα, and MCP-1 levels in the colon (E). A and C Original magnification x100, D x200. Each symbol represents 1 animal. Data are presented as mean±SEM; *p<0.05, **p<0.01, ***p<0.001. Scale bars represent 1mm. nr = number, AU = Arbitrary units, FoxP3 = Forkhead box P3 (marker for regulatory T-cells).

From: Hamers AAJ, van Dam L, Teixeira Duarte JM, Vos M, Marinković G, van Tiel CM, et al. (2015) Deficiency of Nuclear Receptor Nur77 Aggravates Mouse Experimental Colitis by Increased NF?B Activity in Macrophages.
PLoS ONE 10(8): e0133598.

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F4/80 Antibody | Cl:A3-1 gallery image 30

Published customer image:
Rat anti MouseF4/80 antibody, clone A3-1 used for the identification of macrophages in murine kidney by immunofluorescence and immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Clodronate liposome mediated macrophage depletion in Col4a3KO mice. (A) KO male mice were dosed intraperitonally with CL (KO+CL) or PBSL (KO+PBSL) as control. Animals were entered in the study at the age of 4 weeks and were continually dosed until the age of 8 weeks. Injections were repeated every second day, except for the first two doses, which were injected on consecutive days. (B-D) CL significantly reduced F4/80 positive macrophage infiltrates in KO+CL kidney as compared to KO+PBS or KO mice at the age of 8 weeks. (B, C) Representative images and quantitative assessment of F4/80 stained macrophages in kidney sections from KO+CL and KO+PBSL reveal marked reduction in macrophage infiltrates following CL dosing. Scale bar: 100 μm. (D) Real-time PCR analysis of F4/80 mRNA expression in KO+CL, KO+PBSL, KO, and WT littermates at the age of 8 weeks. Significant reduction in F4/80 mRNA expression following CL dosing was observed. PBSL did not affect endogenous F4/80 mRNA expression as shown by similar F4/80 mRNA expression in KO and KO+PBSL mice. (E) Real-time PCR analysis of CD204 and CD206 mRNA showed significant reduction in the expression of both genes in KO kidney upon CL treatment. (F) Representative images and quantitative assessment of F4/80 and CD206 stained kidney sections from KO+CL and KO+PBSL revealed marked reduction in M1 (F4/80+CD206-) and M2 (F4/80+CD206+) macrophages. Asterisk: F4/80+CD206- M1 macrophages, arrow: F4/80+CD206+ M2 macrophages. Scale bar: 20 μm. Welch corrected ANOVA followed by posthoc pairwise t-test with a Bonferroni correction (C,D,E) and unpaired t-test (F) was used to evaluate differences between groups. (C,D,E,F) n = 5 mice per group. CL: clodronate liposomes; PBSL: PBS liposomes.

From: Kim M, Piaia A, Shenoy N, Kagan D, Gapp B, Kueng B, et al. (2015) Progression of Alport Kidney Disease in Col4a3 Knock Out Mice Is Independent of Sex or Macrophage Depletion by Clodronate Treatment.
PLoS ONE 10(11): e0141231.

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F4/80 Antibody | Cl:A3-1 gallery image 31

Published customer image:
Rat anti Mouse F4/80 antibody, clone A3-1 used for the identification of murine macrophages in the kidney by immunohistochemistry on formalin fixed cryosections.
Image caption:
Immunostainings of macrophages and neutrophils in the kidneys of M-Rac1 FC and KO mice. (A) Representative micrographs of F4/80 immunostaining in the kidneys of Vehicle- or LPS-injected M-Rac1 FC and KO mice. Original magnification x 200. (B) Quantitative analysis of F4/80-positive macrophages. Data are means ± s.e.m. Statistical analysis was performed by two-way ANOVA, n.s. genotype effect, P < 0.01 treatment effect, n.s. interaction effect. **P < 0.01 by Bonferroni's post hoc test. n = 5 per each group. (C) Representative micrographs of Ly-6B.2 (mAb 7/4) immunostaining. (D) Quantitative analysis of Ly-6B.2-positive neutrophils. Statistical analysis was performed by two-way ANOVA, n.s. genotype effect, P < 0.01 treatment effect, n.s. interaction effect. **P < 0.01 by Bonferroni's post hoc test. n = 6 per group.

From: Citation: Nagase M, Kurihara H, Aiba A, Young MJ, Sakai T (2016)
Deletion of Rac1GTPase in the Myeloid Lineage Protects against Inflammation-Mediated Kidney Injury in Mice.
PLoS ONE 11(3): e0150886.

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F4/80 Antibody | Cl:A3-1 gallery image 32

Published customer image:
Alexa Fluor 647 conjugated Rat anti Mouse F4/80 antibody, clone A3-1 (MCA497A647) used for the detection of murine Kupffer cells by immunogluorescence
Image caption:
Hepatic stellate cells are the major source of CXCL1, as shown by both quantification of secretion and in situ localization. (A) Quantification of CXCL1 secretion in enriched fractions of hepatocytes, KCs, LSECs and HSCs, freshly isolated and stimulated in vitro with LPS (1 ng/mL LPS, black squares) during 24 hours. Data are representative of three separate experiments with six mice in each group; #P<.05. (B) In-situ localization of CXCL1 in the liver. Immunofluorescent detection for CXCL1 (red) and liver cells nuclei (blue) for nuclei first shows CXCL1 expression in the sinusoids throughout liver parenchyma. (C) Higher resolution shows that CXCL1 (red) is expressed by sub-endothelial cells, which also store retinol droplets in separate compartments, as shown by CRBP1 staining (green). The Cellular Retinol Binding Protein-1 (CRBP-1) is the best marker to detect simultaneously both resting (Glial Fibrillary Acidic Protein, GFAP+) and activated (α-Smooth Muscle Actin, αSMA+) stellate cells in situ. Alexa Fluor-546-CXCL1 (red) staining does not colocalize either with Tie2-GFP in LSECs (green, upper panel), or F4/80 in KCs (blue, middle panel), but with AlexaFluor-488-CRBP1 (green, lower panel), staining both resting and activated HSCs. TOPRO3 was used for nuclei vizualisation.

From: Bigorgne AE, John B, Ebrahimkhani MR, Shimizu-Albergine M, Campbell JS, Crispe IN (2016)
TLR4-Dependent Secretion by Hepatic Stellate Cells of the Neutrophil-Chemoattractant CXCL1 Mediates Liver Response to Gut Microbiota.
PLoS ONE 11(3): e0151063.

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F4/80 Antibody | Cl:A3-1 gallery image 33

Figure A. RPE conjugated mouse anti mouse CD14 (MCA2745PE) and FITC conjugated rat IgG2b isotype control (MCA6006F). Figure B. RPE conjugated mouse anti mouse CD14 (MCA2745PE) and FITC conjugated rat anti mouse F4/80 (MCA497F). All experiments performed on murine peritoneal macrophages in the presence of murine SeroBlock (BUF041A).

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  • Rat anti Mouse F4/80:Alexa Fluor® 488
  • Rat anti Mouse F4/80
  • Rat anti Mouse F4/80:RPE
  • Rat anti Mouse F4/80
  • Rat anti Mouse F4/80:Alexa Fluor® 700
  • Rat anti Mouse F4/80
  • Rat anti Mouse F4/80:RPE-Alexa Fluor® 750
  • Rat anti Mouse F4/80:RPE
  • Rat anti Mouse F4/80
  • Rat anti Mouse F4/80:Low Endotoxin
  • Rat anti Mouse F4/80:RPE-Alexa Fluor® 750
  • Rat anti Mouse F4/80:Biotin
  • Rat anti Mouse F4/80:Biotin
  • Rat anti Mouse F4/80:FITC
  • Rat anti Mouse F4/80:RPE-Alexa Fluor® 647
  • Rat anti Mouse F4/80:APC
  • Rat anti Mouse F4/80:FITC
  • Rat anti Mouse F4/80:Pacific Blue®
  • Rat anti Mouse F4/80:Alexa Fluor® 488
  • Rat anti Mouse F4/80:FITC
  • Rat anti Mouse F4/80:Pacific Blue®
  • Rat anti Mouse F4/80:RPE-Alexa Fluor® 647
  • Rat anti Mouse F4/80:Biotin
  • Rat anti Mouse F4/80:FITC
  • Rat anti Mouse F4/80:APC
  • Rat anti Mouse F4/80:Alexa Fluor® 647
  • Rat anti Mouse F4/80
  • Rat anti Mouse F4/80:Alexa Fluor® 647
  • Rat anti Mouse F4/80:Alexa Fluor® 700
(Rated 5.0 out of 5 based on 1 customer reviews)
    F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.
    F4/80 antibody price reduction stamp
    • Product Type
      Monoclonal Antibody
    • Clone
      Cl:A3-1
    • Isotype
      IgG2b
    13 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      MCA497GAC, EM, F, IF, IP, P *, R, RE, WBdatasheet pdfdatasheet pdf0.1 mg
      MCA497GA
      MCA497BFdatasheet pdfdatasheet pdf0.1 mg
      MCA497B
      MCA497FF, IFdatasheet pdfdatasheet pdf0.1 mg
      MCA497F
      MCA497RC, EM, F, IF, IP, P *, R, RE, WBdatasheet pdfdatasheet pdf0.25 mg
      MCA497R
      MCA497A488TFdatasheet pdfdatasheet pdf0.25 ml
      MCA497A488T
      MCA497GC, EM, F, IF, IP, P *, R, RE, WBdatasheet pdfdatasheet pdf0.5 mg
      MCA497G
      MCA497ELC, F, FN, IP, P *, R, RE, WBdatasheet pdfdatasheet pdf0.5 mg
      MCA497EL
      MCA497BBFdatasheet pdfdatasheet pdf0.5 mg
      MCA497BB
      MCA497FBF, IFdatasheet pdfdatasheet pdf0.5 mg
      MCA497FB
      MCA497PEFdatasheet pdfdatasheet pdf100 Tests
      MCA497PE
      MCA497APCFdatasheet pdfdatasheet pdf100 Tests
      MCA497APC
      MCA497A488Fdatasheet pdfdatasheet pdf100 Tests/1ml
      MCA497A488
      MCA497P750Fdatasheet pdfdatasheet pdf100 Tests/1ml
      MCA497P750
      MCA497P647Fdatasheet pdfdatasheet pdf100 Tests/1ml
      MCA497P647
      MCA497PBFdatasheet pdfdatasheet pdf100 Tests/1ml
      MCA497PB
      MCA497A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
      MCA497A647
      MCA497A700Fdatasheet pdfdatasheet pdf100 Tests/1ml
      MCA497A700
      MCA497C, EM, F, IF, IP, P *, R, REdatasheet pdfdatasheet pdf2 ml
      MCA497
      MCA497RTC, EM, F, IF, IP, P *, R, RE, WBdatasheet pdfdatasheet pdf25 µg
      MCA497RT
      MCA497FTF, IFdatasheet pdfdatasheet pdf25 µg
      MCA497FT
      MCA497BTFdatasheet pdfdatasheet pdf25 µg
      MCA497BT
      MCA497PETF, IFdatasheet pdfdatasheet pdf25 Tests
      MCA497PET
      MCA497P750TFdatasheet pdfdatasheet pdf25 Tests
      MCA497P750T
      MCA497P647TFdatasheet pdfdatasheet pdf25 Tests
      MCA497P647T
      MCA497A700TFdatasheet pdfdatasheet pdf25 Tests/0.25ml
      MCA497A700T
      MCA497APCTFdatasheet pdfdatasheet pdf25 Tests/0.25ml
      MCA497APCT
      MCA497PBTFdatasheet pdfdatasheet pdf25 Tests/0.25ml
      MCA497PBT
      MCA497A647TFdatasheet pdfdatasheet pdf25 Tests/0.25ml
      MCA497A647T
      MCA497FAF, IFdatasheet pdfdatasheet pdf50 µg
      MCA497FA
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Rat anti mouse F4/80 antibody, clone Cl:A3-1 recognises the murine F4/80 antigen, a ~160 kDa cell surface glycoprotein member of the EGF-TM7 family of proteins which shares 68% overall amino acid identity with human EGF module-containing mucin-like hormone receptor 1 (EMR1).

        Expression of F4/80 is heterogeneous and is modulated during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans cells, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen (Hume, et al. 1984). F4/80 antigen is also expressed on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node (Gordon, et al. 1994). The ligands and biological functions of the F4/80 antigen have not been fully determined but a role for F4/80 in the generation of efferent CD8+ve regulatory T cells is proposed (Lin, et al. 2005)

        Rat anti mouse F4/80 antibody, clone Cl:A3-1 modulates cytokine levels released in response to Listeria monocytogenes (Warschkau & Kiderlen, 1999).

        A Human anti-idiotypic CI:A31 antibody, clone 17867 (HCA154 ) which binds to and blocks activity of Rat anti mouse F4/80 antibody, clone Cl:A3-1 is also available for use as a control in experiments utilizing clone A3-1.
      • Intended Use
      • Target Species
        Mouse
      • Product Form
        Purified IgG conjugated to Alexa Fluor® 488 - liquid
        Purified IgG - liquid
        Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
        Purified IgG - liquid
        Purified IgG conjugated to Alexa Fluor® 700 - liquid
        Purified IgG - liquid
        Purified IgG conjugated to R. Phycoerythrin (RPE)-Alexa Fluor® 750 - lyophilised
        Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG conjugated to R. Phycoerythrin (RPE)-Alexa Fluor® 750 - lyophilised
        Purified IgG conjugated to Biotin - liquid
        Purified IgG conjugated to Biotin - liquid
        Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid.
        Purified IgG conjugated to R. Phycoerythrin (RPE)-Alexa Fluor® 647 - lyophilised
        Purified IgG conjugated to Allophycocyanin (APC) - lyophilised
        Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid.
        Purified IgG conjugated to Pacific Blue® - liquid
        Purified IgG conjugated to Alexa Fluor® 488 - liquid
        Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid.
        Purified IgG conjugated to Pacific Blue® - liquid
        Purified IgG conjugated to R. Phycoerythrin (RPE)-Alexa Fluor® 647 - lyophilised
        Purified IgG conjugated to Biotin - liquid
        Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid.
        Purified IgG conjugated to Allophycocyanin (APC) - lyophilised
        Purified IgG conjugated to Alexa Fluor® 647 - liquid
        Tissue Culture Supernatant - liquid
        Purified IgG conjugated to Alexa Fluor® 647 - liquid
        Purified IgG conjugated to Alexa Fluor® 700 - liquid
      • Reconstitution
        Pack Size: 100 TestsReconstitute with 1 ml distilled water
        Pack Size: 25 TestsReconstitute with 0.25 ml distilled water
        Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
        Pack Size: 100 Tests/1mlReconstitute with 1.0 ml distilled water
        Pack Size: 100 TestsReconstitute with 1 ml distilled water
        Pack Size: 25 TestsReconstitute with 0.25 ml distilled water
        Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
        Pack Size: 100 Tests/1mlReconstitute with 1.0 ml distilled water
        Pack Size: 100 Tests/1mlReconstitute with 1.0 ml distilled water.
        Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
        Pack Size: 100 TestsReconstitute with 1 ml distilled water
        Pack Size: 25 Tests/0.25mlReconstitute with 0.25ml distilled water
        Pack Size: 100 Tests/1mlReconstitute with 1.0 ml distilled water.
        Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
        Pack Size: 100 TestsReconstitute with 1 ml distilled water
        Pack Size: 25 Tests/0.25mlReconstitute with 0.25ml distilled water
      • Preparation
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
        Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
        Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
        Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
        Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
        Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      • Preservative Stabilisers
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        5%Sucrose
        0.09%Sodium Azide
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        5%Sucrose
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        5%Sucrose
        0.09%Sodium Azide
        None present
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        5%Sucrose
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        5%Sucrose
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        5%Sucrose
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        5%Sucrose
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        5%Sucrose
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
      • Immunogen
        Thioglycollate stimulated peritoneal macrophages from C57BL/6 mice.
      • Purity
      • Approx. Protein Concentrations
        IgG concentration 0.05 mg/ml
        IgG concentration 1.0 mg/ml
        IgG concentration 1.0 mg/ml
        IgG concentration 0.05 mg/ml
        IgG concentration 1.0 mg/ml
        IgG concentration 1.0 mg/ml
        IgG concentration 1 mg/ml
        Pack Size: 25 µg, 0.1 mgIgG concentration 0.1 mg/ml
        Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
        Pack Size: 25 µg, 0.1 mgIgG concentration 0.1 mg/ml
        Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
        Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
        Pack Size: 25 µg, 50 µg, 0.1 mgIgG concentration 0.1 mg/ml
        Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
        Pack Size: 25 µg, 50 µg, 0.1 mgIgG concentration 0.1 mg/ml
        IgG concentration 0.05 mg/ml
        IgG concentration 0.05 mg/ml
        Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
        Pack Size: 25 µg, 50 µg, 0.1 mgIgG concentration 0.1 mg/ml
        IgG concentration 0.05 mg/ml
        Pack Size: 25 µg, 0.1 mgIgG concentration 0.1 mg/ml
        Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
        Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
        Pack Size: 25 µg, 50 µg, 0.1 mgIgG concentration 0.1 mg/ml
        IgG concentration 0.05 mg/ml
        IgG concentration 0.05 mg/ml
        IgG concentration 0.05 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        None present
        Phosphate buffered saline
        Phosphate buffered saline
      • Fusion Partners
        Spleen cells from immunised HOB2 rats were fused with cells of the mouse NS1 myeloma cell line.
      • Storage
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Pack Size: 100 TestsPrior to reconstitution store at +4oC. Following reconstitution store at +4oC.

        DO NOT FREEZE.

        This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
        Pack Size: 25 TestsPrior to reconstitution store at +4oC.
        After reconstitution store at +4oC.
        DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC.

        DO NOT FREEZE.

        This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
        Pack Size: 100 TestsPrior to reconstitution store at +4oC. Following reconstitution store at +4oC.

        DO NOT FREEZE.

        This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
        Pack Size: 25 TestsPrior to reconstitution store at +4oC.
        After reconstitution store at +4oC.
        DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at -20oC only.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC.

        DO NOT FREEZE.

        This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
        Pack Size: 25 µg, 0.1 mgStore at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Pack Size: 0.5 mgStore at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Pack Size: 25 µg, 0.1 mgStore at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Pack Size: 0.5 mgStore at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Pack Size: 0.5 mgStore at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Pack Size: 25 µg, 50 µg, 0.1 mgStore at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC.

        DO NOT FREEZE.

        This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
        Pack Size: 100 TestsPrior to reconstitution store at +4oC. Following reconstitution store at +4oC.

        DO NOT FREEZE.

        This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
        Pack Size: 25 Tests/0.25mlStore at +4oC. DO NOT FREEZE.
        This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
        Pack Size: 0.5 mgStore at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Pack Size: 25 µg, 50 µg, 0.1 mgStore at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Pack Size: 0.5 mgStore at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Pack Size: 25 µg, 50 µg, 0.1 mgStore at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC.

        DO NOT FREEZE.

        This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
        Pack Size: 25 µg, 0.1 mgStore at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Pack Size: 0.5 mgStore at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Pack Size: 0.5 mgStore at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Pack Size: 25 µg, 50 µg, 0.1 mgStore at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Pack Size: 100 TestsPrior to reconstitution store at +4oC. Following reconstitution store at +4oC.

        DO NOT FREEZE.

        This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
        Pack Size: 25 Tests/0.25mlStore at +4oC. DO NOT FREEZE.
        This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        18 months from date of despatch.
        18 months from date of despatch.
        12 months from date of reconstitution.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        12 months from date of reconstitution.
        12 months from date of reconstitution.
        18 months from date of despatch.
        18 months from date of despatch.
        12 months from date of reconstitution.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        12 months from date of reconstitution.
        12 months from date of reconstitution.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        12 months from date of reconstitution.
        18 months from date of despatch.
        18 months from date of despatch.
        12 months from date of reconstitution.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
      • GO Terms
        external side of plasma membrane
        integral to membrane
        calcium ion binding
        G-protein coupled receptor activity
        neuropeptide signaling pathway
      • UniProt
      • Entrez Gene
      • Acknowledgements
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
        This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
        This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
        This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
        This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        Flow CytometryNeat1/10
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry1/501/100
        Immuno-electron Microscopy
        Immunofluorescence
        Immunohistology - Frozen
        Immunohistology - Resin
        Immunoprecipitation
        Radioimmunoassays
        Western Blotting
        Immunohistology - Paraffin(1)
        (1)
        F4/80 antibody requires pre-treatment of paraffin sections prior to staining. Proteinase K is recommended for tissues fixed for less than 24 hours. Citrate buffer pH 6.0 is recommended for tissues fixed for more than 24 hours. Please view the protocol at https://www.bio-rad-antibodies.com/f480-pre-staining-antigen-unmasking.html.
      • Application NameYesNoMin DilutionMax Dilution
        Flow CytometryNeat
        Immunofluorescence
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry1/501/100
        Immuno-electron Microscopy
        Immunofluorescence
        Immunohistology - Frozen
        Immunohistology - Resin
        Immunoprecipitation
        Radioimmunoassays
        Western Blotting
        Immunohistology - Paraffin(1)
        (1)
        F4/80 antibody requires pre-treatment of paraffin sections prior to staining. Proteinase K is recommended for tissues fixed for less than 24 hours. Citrate buffer pH 6.0 is recommended for tissues fixed for more than 24 hours. Please view the protocol at https://www.bio-rad-antibodies.com/f480-pre-staining-antigen-unmasking.html.
      • Application NameYesNoMin DilutionMax Dilution
        Flow CytometryNeat1/10
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry1/501/100
        Immuno-electron Microscopy
        Immunofluorescence
        Immunohistology - Frozen
        Immunohistology - Resin
        Immunoprecipitation
        Radioimmunoassays
        Western Blotting
        Immunohistology - Paraffin(1)
        (1)
        F4/80 antibody requires pre-treatment of paraffin sections prior to staining. Proteinase K is recommended for tissues fixed for less than 24 hours. Citrate buffer pH 6.0 is recommended for tissues fixed for more than 24 hours. Please view the protocol at https://www.bio-rad-antibodies.com/f480-pre-staining-antigen-unmasking.html.
      • Application NameYesNoMin DilutionMax Dilution
        Flow CytometryNeat
      • Application NameYesNoMin DilutionMax Dilution
        Flow CytometryNeat
        Immunofluorescence
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry1/501/100
        Immuno-electron Microscopy
        Immunofluorescence
        Immunohistology - Frozen
        Immunohistology - Resin
        Immunoprecipitation
        Radioimmunoassays
        Western Blotting
        Immunohistology - Paraffin(1)
        (1)
        F4/80 antibody requires pre-treatment of paraffin sections prior to staining. Proteinase K is recommended for tissues fixed for less than 24 hours. Citrate buffer pH 6.0 is recommended for tissues fixed for more than 24 hours. Please view the protocol at https://www.bio-rad-antibodies.com/f480-pre-staining-antigen-unmasking.html.
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry1/1001/200
        Functional Assays
        Immunohistology - Frozen
        Immunohistology - Resin
        Immunoprecipitation
        Radioimmunoassays
        Western Blotting
        Immunohistology - Paraffin(1)
        (1)
        This product requires pre-treatment of paraffin sections prior to staining. Proteinase K is recommended for tissues fixed for less than 24 hours. Citrate buffer pH 6.0 is recommended for tissues fixed for more than 24 hours.

        A recommended protocol for the use of this antibody on paraffin-embedded tissues can be found at: www.bio-rad-antibodies.com/support/recommended-protocols-667.html
      • Application NameYesNoMin DilutionMax Dilution
        Flow CytometryNeat
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry
        Neat Pack Size: 0.1 mg, 25 µg
        1/10 Pack Size: 0.5 mg
        1/10Pack Size: 0.1 mg, 25 µg
        1/50Pack Size: 0.5 mg
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry
        Neat Pack Size: 0.1 mg, 25 µg
        1/10 Pack Size: 0.5 mg
        1/10Pack Size: 0.1 mg, 25 µg
        1/50Pack Size: 0.5 mg
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry
        NeatPack Size: 50 µg
        1/10 Pack Size: 0.5 mg
        NeatPack Size: 0.1 mg, 25 µg
        1/10Pack Size: 50 µg
        1/50Pack Size: 0.5 mg
        Immunofluorescence
      • Application NameYesNoMin DilutionMax Dilution
        Flow CytometryNeat
      • Application NameYesNoMin DilutionMax Dilution
        Flow CytometryNeat1/10
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry
        NeatPack Size: 50 µg
        1/10 Pack Size: 0.5 mg
        NeatPack Size: 0.1 mg, 25 µg
        1/10Pack Size: 50 µg
        1/50Pack Size: 0.5 mg
        Immunofluorescence
      • Application NameYesNoMin DilutionMax Dilution
        Flow CytometryNeat1/5
      • Application NameYesNoMin DilutionMax Dilution
        Flow CytometryNeat1/10
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry
        NeatPack Size: 50 µg
        1/10 Pack Size: 0.5 mg
        NeatPack Size: 0.1 mg, 25 µg
        1/10Pack Size: 50 µg
        1/50Pack Size: 0.5 mg
        Immunofluorescence
      • Application NameYesNoMin DilutionMax Dilution
        Flow CytometryNeat1/5
      • Application NameYesNoMin DilutionMax Dilution
        Flow CytometryNeat
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry
        Neat Pack Size: 0.1 mg, 25 µg
        1/10 Pack Size: 0.5 mg
        1/10Pack Size: 0.1 mg, 25 µg
        1/50Pack Size: 0.5 mg
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry
        NeatPack Size: 50 µg
        1/10 Pack Size: 0.5 mg
        NeatPack Size: 0.1 mg, 25 µg
        1/10Pack Size: 50 µg
        1/50Pack Size: 0.5 mg
        Immunofluorescence
      • Application NameYesNoMin DilutionMax Dilution
        Flow CytometryNeat1/10
      • Application NameYesNoMin DilutionMax Dilution
        Flow CytometryNeat1/10
      • Application NameYesNoMin DilutionMax Dilution
        Flow CytometryNeat1/10
        Immuno-electron Microscopy
        Immunofluorescence
        Immunohistology - Frozen
        Immunohistology - Resin
        Immunoprecipitation
        Radioimmunoassays
        Immunohistology - Paraffin(1)
        (1)
        F4/80 antibody requires pre-treatment of paraffin sections prior to staining. Proteinase K is recommended for tissues fixed for less than 24 hours. Citrate buffer pH 6.0 is recommended for tissues fixed for more than 24 hours. Please view the protocol at https://www.bio-rad-antibodies.com/f480-pre-staining-antigen-unmasking.html.
      • Application NameYesNoMin DilutionMax Dilution
        Flow CytometryNeat1/10
      • Application NameYesNoMin DilutionMax Dilution
        Flow CytometryNeat1/10

      • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
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      • Technical Advice
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      • Technical Advice
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      • Technical Advice
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      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Flow Cytometry
        Use 10ul of the suggested working dilution to label 106 cells in 100ul.
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use

      Additional F4/80 Antibody Formats

      Formats Clone Applications Sizes available
      F4/80 Antibody : Alexa Fluor® 700 Cl:A3-1 F 25 Tests/0.25ml | 100 Tests/1ml
      F4/80 Antibody : Alexa Fluor® 647 Cl:A3-1 F 25 Tests/0.25ml | 100 Tests/1ml
      F4/80 Antibody : RPE-Alexa Fluor® 647 Cl:A3-1 F 100 Tests/1ml | 25 Tests
      F4/80 Antibody : Purified Cl:A3-1 C, EM, F, IF, IP, P *, R, RE, WB 25 µg | 0.1 mg | 0.25 mg | 0.5 mg
      F4/80 Antibody : RPE-Alexa Fluor® 750 Cl:A3-1 F 100 Tests/1ml | 25 Tests
      F4/80 Antibody : RPE Cl:A3-1 F, IF 100 Tests | 25 Tests
      F4/80 Antibody : APC Cl:A3-1 F 100 Tests | 25 Tests/0.25ml
      F4/80 Antibody : Biotin Cl:A3-1 F 0.5 mg | 0.1 mg | 25 µg
      F4/80 Antibody : Alexa Fluor® 488 Cl:A3-1 F 100 Tests/1ml | 0.25 ml
      F4/80 Antibody : FITC Cl:A3-1 F, IF 50 µg | 0.1 mg | 25 µg | 0.5 mg
      F4/80 Antibody : Pacific Blue® Cl:A3-1 F 100 Tests/1ml | 25 Tests/0.25ml
      F4/80 Antibody : Low Endotoxin Cl:A3-1 C, F, FN, IP, P *, R, RE, WB 0.5 mg
      F4/80 Antibody : S/N Cl:A3-1 C, EM, F, IF, IP, P *, R, RE 2 ml
      • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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        Streptavidin:APCSTAR119100 TestsF
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        Recommended Negative Isotype Control

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat IgG2b Negative Control:Alexa Fluor® 700MCA1125A700100 Tests/1mlF
          MCA1125A700
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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          MCA6006P750
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          MCA6006P750
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          Rat IgG2b Negative Control:Alexa Fluor® 700MCA1125A700100 Tests/1mlF
          MCA1125A700

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Mouse Seroblock FcRBUF041A0.1 mgF
            BUF041A
            Mouse Seroblock FcRBUF041B0.5 mgF
            BUF041B
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
            BUF025C
            Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
            BUF025A
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Mouse Seroblock FcRBUF041A0.1 mgF
            BUF041A
            Mouse Seroblock FcRBUF041B0.5 mgF
            BUF041B
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
            BUF025C
            Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
            BUF025A
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Mouse Seroblock FcRBUF041A0.1 mgF
            BUF041A
            Mouse Seroblock FcRBUF041B0.5 mgF
            BUF041B
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Mouse Seroblock FcRBUF041A0.1 mgF
            BUF041A
            Mouse Seroblock FcRBUF041B0.5 mgF
            BUF041B
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Mouse Seroblock FcRBUF041A0.1 mgF
            BUF041A
            Mouse Seroblock FcRBUF041B0.5 mgF
            BUF041B
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Mouse Seroblock FcRBUF041A0.1 mgF
            BUF041A
            Mouse Seroblock FcRBUF041B0.5 mgF
            BUF041B
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Mouse Seroblock FcRBUF041A0.1 mgF
            BUF041A
            Mouse Seroblock FcRBUF041B0.5 mgF
            BUF041B
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Mouse Seroblock FcRBUF041A0.1 mgF
            BUF041A
            Mouse Seroblock FcRBUF041B0.5 mgF
            BUF041B
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Mouse Seroblock FcRBUF041A0.1 mgF
            BUF041A
            Mouse Seroblock FcRBUF041B0.5 mgF
            BUF041B
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Mouse Seroblock FcRBUF041A0.1 mgF
            BUF041A
            Mouse Seroblock FcRBUF041B0.5 mgF
            BUF041B

            Recommended Positive Controls

              Histology Controls

                Source Reference

                Antibody Characterization Reference

                References

                Write your review

                Excellent
                5/5stars
                by
                Submitted: 2 Mar 2015
                This antibody clone gave me the best F4/80 staining I've ever achieved. It was a bright membrane staining in the correct anatomical location. Briefly, 10um OCT frozen liver tissue sections were air dried for 10 min. at room temperature, before being fixed for 45 min. with Histochoice MB. Sections were then blocked with 10% normal goat sera with 6% bovine serum albumin for 1 hour at room temperature. Primary antibody was added at 1:100 in blocking buffer overnight at 4C. A goat anti-mouse secondary conjugated to Qdot 655 was added at 20 mM in 6% BSA for 1 hour at room temperature. Sections were counterstained with 300 nM DAPI and mounted in HistoMount. Images were taken on a Zeiss AxioPlan II at 40X.

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