F4/80 antibody | Cl:A3-1

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Rat anti Mouse F4/80:Alexa Fluor® 488

F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.

Rat anti Mouse F4/80:Alexa Fluor® 647

F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.

Rat anti Mouse F4/80:APC

F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.

Rat anti Mouse F4/80:Biotin

F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.

Rat anti Mouse F4/80:FITC

F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.

Rat anti Mouse F4/80:Low Endotoxin

F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.

Rat anti Mouse F4/80:Pacific Blue®

F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.

Rat anti Mouse F4/80

F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.

Rat anti Mouse F4/80:RPE

F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.

Rat anti Mouse F4/80:RPE-Alexa Fluor® 647

F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.

Rat anti Mouse F4/80:RPE-Alexa Fluor® 750

F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.

Rat anti Mouse F4/80

F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.

Rat anti Mouse F4/80:Alexa Fluor® 700

F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. Bio-Rad (formerly AbD Serotec) is the only manufacturer of clone CI:A3-1, giving us a wide range of formats.

Product Type
Monoclonal Antibody
Clone
Cl:A3-1
Isotype
IgG2b
F4/80 antibody price reduction stamp
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA497A488T F 0.25 ml
MCA497A488 F 100 Tests/1ml
MCA497A647T F 25 Tests/0.25ml
MCA497A647 F 100 Tests/1ml
MCA497APCT F 25 Tests/0.25ml
MCA497APC F 100 Tests
MCA497BT F 25 µg
MCA497B F 0.1 mg
MCA497BB F 0.5 mg
MCA497FT F IF 25 µg
MCA497FA F IF 50 µg
MCA497F F IF 0.1 mg
MCA497FB F IF 0.5 mg
MCA497EL C F FN IP P* R RE WB 0.5 mg
MCA497PBT F 25 Tests/0.25ml
MCA497PB F 100 Tests/1ml
MCA497RT C EM F IF IP P* R RE WB 25 µg
MCA497GA C EM F IF IP P* R RE WB 0.1 mg
MCA497R C EM F IF IP P* R RE WB 0.25 mg
MCA497G C EM F IF IP P* R RE WB 0.5 mg
MCA497PET F IF 25 Tests
MCA497PE F 100 Tests
MCA497P647T F 25 Tests
MCA497P647 F 100 Tests/1ml
MCA497P750T F 25 Tests
MCA497P750 F 100 Tests/1ml
MCA497 C EM F IF IP P* R RE 2 ml
MCA497A700T F 25 Tests/0.25ml
MCA497A700 F 100 Tests/1ml
Rat anti mouse F4/80 antibody, clone Cl:A3-1 recognises the murine F4/80 antigen, a ~160 kDa cell surface glycoprotein member of the EGF-TM7 family of proteins which shares 68% overall amino acid identity with human EGF module-containing mucin-like hormone receptor 1 (EMR1).

Expression of F4/80 is heterogeneous and is modulated during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans cells, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen (Hume, et al. 1984). F4/80 antigen is also expressed on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node (Gordon, et al. 1994). The ligands and biological functions of the F4/80 antigen have not been fully determined but a role for F4/80 in the generation of efferent CD8+ve regulatory T cells is proposed (Lin, et al. 2005)

Rat anti mouse F4/80 antibody, clone Cl:A3-1 modulates cytokine levels released in response to Listeria monocytogenes (Warschkau & Kiderlen, 1999).

A Human anti-idiotypic CI:A31 antibody, clone 17867 (HCA154 ) which binds to and blocks activity of Rat anti mouse F4/80 antibody, clone Cl:A3-1 is also available for use as a control in experiments utilizing clone A3-1.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Alexa Fluor® 488 - liquid
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Product Form
Purified IgG conjugated to Allophycocyanin (APC) - lyophilised
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to Pacific Blue® - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE)-Alexa Fluor® 647 - lyophilized
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE)-Alexa Fluor® 750 - lyophilized
Product Form
Tissue Culture Supernatant - liquid
Product Form
Purified IgG conjugated to Alexa Fluor® 700 - liquid
Reconstitution
Pack Size: 25 Tests/0.25ml
Reconstitute with 0.25ml distilled water
Pack Size: 100 Tests
Reconstitute with 1 ml distilled water
Reconstitution
Pack Size: 25 Tests
Reconstitute with 0.25 ml distilled water
Pack Size: 100 Tests
Reconstitute with 1 ml distilled water
Reconstitution
Pack Size: 25 Tests
Reconstitute in 0.25 ml disilled water
Pack Size: 100 Tests/1ml
Reconstitute with 1.0 ml distilled water
Reconstitution
Pack Size: 25 Tests
Reconstitute in 0.25 ml disilled water
Pack Size: 100 Tests/1ml
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Preparation
Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
None present
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
None present
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Carrier Free
Yes
Carrier Free
Yes
Immunogen
Thioglycollate stimulated peritoneal macrophages from C57BL/6 mice.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
Pack Size: 25 µg, 0.1 mg
IgG concentration 0.1 mg/ml
Pack Size: 0.5 mg
IgG concentration 0.5 mg/ml
Approx. Protein Concentrations
Pack Size: 25 µg, 50 µg, 0.1 mg
IgG concentration 0.1 mg/ml
Pack Size: 0.5 mg
IgG concentration 0.5 mg/ml
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Spleen cells from immunised HOB2 rats were fused with cells of the mouse NS1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Pack Size: 25 Tests/0.25ml
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 100 Tests
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Pack Size: 25 µg, 0.1 mg
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 0.5 mg
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Pack Size: 25 µg, 50 µg, 0.1 mg
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 0.5 mg
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at -20oC only.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Pack Size: 25 Tests
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
Pack Size: 100 Tests
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of reconstitution.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch
Shelf Life
12 months from date of reconstitution.
Shelf Life
12 months from date of reconstitution.
Shelf Life
12 months from date of reconstitution.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q61549 Related reagents
Entrez Gene
Emr1 Related reagents
GO Terms
GO:0005509 calcium ion binding
GO:0016021 integral to membrane
GO:0004930 G-protein coupled receptor activity
GO:0007218 neuropeptide signaling pathway
GO:0009897 external side of plasma membrane
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of F4/80 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry Neat 1/10
Flow Cytometry Neat 1/10
Flow Cytometry Neat Pack Size: 25 µg, 0.1 mg
1/10 Pack Size: 0.5 mg
1/10 Pack Size: 25 µg, 0.1 mg
1/50 Pack Size: 0.5 mg
Flow Cytometry Neat Pack Size: 50 µg
1/10 Pack Size: 0.5 mg
Neat Pack Size: 25 µg, 0.1 mg
1/10 Pack Size: 50 µg
1/50 Pack Size: 0.5 mg
Immunofluorescence
Flow Cytometry 1/100 1/200
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin 1
Immunohistology - Resin
Immunoprecipitation
Radioimmunoassays
Western Blotting
Flow Cytometry Neat 1/5
Flow Cytometry 1/50 1/100
Immuno-electron Microscopy
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 1
Immunohistology - Resin
Immunoprecipitation
Radioimmunoassays
Western Blotting
Flow Cytometry Neat
Immunofluorescence
Flow Cytometry Neat
Flow Cytometry Neat
Flow Cytometry Neat 1/10
Immuno-electron Microscopy
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 1
Immunohistology - Resin
Immunoprecipitation
Radioimmunoassays
Flow Cytometry Neat 1/10
  1. 1 Rat anti Mouse F4/80 antibody, clone A3-1 requires pre-treatment of paraffin sections prior to staining. Proteinase K is recommended for tissues fixed for less than 24 hours. Citrate buffer pH 6.0 is recommended for tissues fixed for more than 24 hours. Please view the protocol at Antigen Retrieval Techniques.
  1. 1 Rat anti Mouse F4/80 antibody, clone A3-1 requires pre-treatment of paraffin sections prior to staining. Proteinase K is recommended for tissues fixed for less than 24 hours. Citrate buffer pH 6.0 is recommended for tissues fixed for more than 24 hours. Please view the protocol at Antigen Retrieval Techniques.
  1. 1 Rat anti Mouse F4/80 antibody, clone A3-1 requires pre-treatment of paraffin sections prior to staining. Proteinase K is recommended for tissues fixed for less than 24 hours. Citrate buffer pH 6.0 is recommended for tissues fixed for more than 24 hours. Please view the protocol at Antigen Retrieval Techniques.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. The suggested working dilution is given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. The suggested working dilution is given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. The suggested working dilution is given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. The suggested working dilution is given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. The suggested working dilution is given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Streptavidin:FITC 710002 1 mg F
Streptavidin:APC STAR119 100 Tests F
Streptavidin:RPE STAR4A 1 ml F
Goat anti Rat IgG (H/L):HRP 305005 1 ml C E IB
Goat anti Rat IgG2b:HRP STAR114P 0.5 mg C E P WB
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F
Goat anti Rat IgG (H/L):HRP 305005 1 ml C E IB
Goat anti Rat IgG2b:HRP STAR114P 0.5 mg C E P WB
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F
Goat anti Rat IgG (H/L):HRP 305005 1 ml C E IB
Goat anti Rat IgG2b:HRP STAR114P 0.5 mg C E P WB
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Rat IgG2b Negative Control:RPE-Alexa Fluor® 750 MCA6006P750 100 Tests/1ml F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F

Application Based External Images

Flow Cytometry

Immuno-electron Microscopy

Immunofluorescence

Immunohistology - Frozen

Immunohistology - Paraffin

Western Blotting

Product Specific References

Source Reference

  1. Austyn, J.M. & Gordon, S. (1981) F4/80, a monoclonal antibody directed specifically against the mouse macrophage.
    Eur J Immunol. 11 (10): 805-15.

Antibody Characterization Reference

  1. Hume, D.A. et al. (1984) The mononuclear phagocyte system of the mouse defined by immunohistochemical localisation of antigen F4/80: macrophages associated with epithelia.
    Anat Rec. 210 (3): 503-12.
  2. Lee, S.H. et al. (1985) Quantitative analysis of total macrophage content in adult mouse tissues. Immunochemical studies with monoclonal antibody F4/80.
    J Exp Med. 161 (3): 475-89.

References for F4/80 antibody

  1. Gordon, S. et al. (1992) Antigen markers of macrophage differentiation in murine tissues.
    Curr Top Microbiol Immunol. 181: 1-37.
  2. Warschkau, H. & Kiderlen, A.F. (1999) A monoclonal antibody directed against the murine macrophage surface molecule F4/80 modulates natural immune response to Listeria monocytogenes.
    J Immunol. 163 (6): 3409-16.
  3. Lin, H.H.et al. (2005) The macrophage F4/80 receptor is required for the induction of antigen-specific efferent regulatory T cells in peripheral tolerance.
    J Exp Med. 201 (10): 1615-25.
  4. Chan, R.J. et al. (2005) Human somatic PTPN11 mutations induce hematopoietic cell hypersensitivity to granulocyte-macrophage colony stimulating factor
    Blood. 105: 3737-3742.
  5. Moore, K.J. et al. (2000) Divergent response to LPS and bacteria in CD14-deficient murine macrophages.
    J Immunol. 165 (8): 4272-80.
  6. Dandekar, A.A.et al. (2004) Bystander CD8 T-cell-mediated demyelination is interferon-gamma-dependent in a coronavirus model of multiple sclerosis.
    Am J Pathol. 164: 363-9.
  7. Muto, A. et al. (2011) Eph-B4 prevents venous adaptive remodeling in the adult arterial environment.
    J Exp Med. 208 (3): 561-75.
  8. Pizza, F.X. et al. (2005) Neutrophils contribute to muscle injury and impair its resolution after lengthening contractions in mice.
    J Physiol. 562 (Pt 3): 899-913.
  9. Tarallo, V. et al. (2011) The biflavonoid amentoflavone inhibits neovascularization preventing the activity of proangiogenic vascular endothelial growth factors.
    J Biol Chem. 286: 19641-51.
  10. Rivollier, A. et al. (2012) Inflammation switches the differentiation program of Ly6Chi monocytes from antiinflammatory macrophages to inflammatory dendritic cells in the colon.
    J Exp Med. 209: 139-55.
  11. Hemmi, H. et al. (2009) A new triggering receptor expressed on myeloid cells (Trem) family member, Trem-like 4, binds to dead cells and is a DNAX activation protein 12-linked marker for subsets of mouse macrophages and dendritic cells.
    J Immunol. 182:1278-86.
  12. Seitz, O. et al. (2010) Wound Healing in Mice with High-Fat Diet- or ob Gene-Induced Diabetes-Obesity Syndromes: A Comparative Study
    Exp Diabetes Res. 2010: 476969.
  13. Miao, E.A. et al. (2011) Caspase-1-induced pyroptosis is an innate immune effector mechanism against intracellular bacteria.
    Nat Immunol. 11: 1136-42.
  14. Wang, X. et al. (2011) Activation of the cholinergic antiinflammatory pathway ameliorates obesity-induced inflammation and insulin resistance
    Endocrinology. 152: 836-46.
  15. Cunningham, O. et al. (2009) Microglia and the urokinase plasminogen activator receptor/uPA system in innate brain inflammation.
    Glia. 57: 1802-14.
  16. Gornicka, A. et al. (2012) Adipocyte hypertrophy is associated with lysosomal permeability both in vivo and in vitro: role in adipose tissue inflammation.
    Am J Physiol Endocrinol Metab. 303: E597-606.
  17. Akbarshahi, H. et al. (2012) Enrichment of Murine CD68(+)CCR2(+) and CD68(+)CD206(+) Lung Macrophages in Acute Pancreatitis-Associated Acute Lung Injury.
    PLoS One. 7: e42654.
  18. Banda NK et al. (2012) Role of C3a receptors, C5a receptors, and complement protein C6 deficiency in collagen antibody-induced arthritis in mice.
    J Immunol. 188 (3): 1469-78.
  19. Bonde, A.K. et al. (2012) Intratumoral macrophages contribute to epithelial-mesenchymal transition in solid tumors.
    BMC Cancer. 12: 35.
  20. Choi, K.M. et al. (2010) CD206-positive M2 macrophages that express heme oxygenase-1 protect against diabetic gastroparesis in mice.
    Gastroenterology. 138 (7): 2399-409, 2409.e1.
  21. Tamaki, S. et al. (2013) Interleukin-16 promotes cardiac fibrosis and myocardial stiffening in heart failure with preserved ejection fraction.
    PLoS One. 8: e68893.
  22. Kihira, Y. et al. (2014) Deletion of hypoxia-inducible factor-1α in adipocytes enhances glucagon-like Peptide-1 secretion and reduces adipose tissue inflammation.
    PLoS One. 9(4):e93856.
  23. Chinzei, N. et al. (2015) P21 deficiency delays regeneration of skeletal muscular tissue.
    PLoS One. 10 (5): e0125765.
  24. Sumiyoshi, M. et al. (2015) Antitumor and antimetastatic actions of xanthoangelol and 4-hydroxyderricin isolated from Angelica keiskei roots through the inhibited activation and differentiation of M2 macrophages.
    Phytomedicine. 22 (7-8): 759-67.
  25. Kim, M. et al. (2015) Progression of Alport Kidney Disease in Col4a3 Knock Out Mice Is Independent of Sex or Macrophage Depletion by Clodronate Treatment.
    PLoS One. 10 (11): e0141231.
  26. Nagase, M. et al. (2016) Deletion of Rac1GTPase in the Myeloid Lineage Protects against Inflammation-Mediated Kidney Injury in Mice.
    PLoS One. 11 (3): e0150886.
  27. Bonaterra, G.A. et al. (2016) Morphological Alterations in Gastrocnemius and Soleus Muscles in Male and Female Mice in a Fibromyalgia Model.
    PLoS One. 11 (3): e0151116.
  28. Lei, B. et al. (2016) Neuroprotective pentapeptide CN-105 improves functional and histological outcomes in a murine model of intracerebral hemorrhage.
    Sci Rep. 6: 34834.
  29. Glastras, S.J. et al. (2017) The renal consequences of maternal obesity in offspring are overwhelmed by postnatal high fat diet.
    PLoS One. 12 (2): e0172644.
  30. Zeng, J. & Howard, J.C. (2010) Spontaneous focal activation of invariant natural killer T (iNKT) cells in mouse liver and kidney.
    BMC Biol. 8: 142.
  31. Pepe, G. et al. (2017) Self-renewal and phenotypic conversion are the main physiological responses of macrophages to the endogenous estrogen surge.
    Sci Rep. 7: 44270.
  32. Kawada, S. et al. (2017) Impairment of cold injury-induced muscle regeneration in mice receiving a combination of bone fracture and alendronate treatment.
    PLoS One. 12 (7): e0181457.
  33. Zhang, M.Z. et al. (2015) Inhibition of cyclooxygenase-2 in hematopoietic cells results in salt-sensitive hypertension.
    J Clin Invest. 125 (11): 4281-94.
  34. Crompton, M. et al. (2017) A mutation in Nischarin causes otitis media via LIMK1 and NF-κB pathways.
    PLoS Genet. 13 (8): e1006969.
  35. Sogawa, Y. et al. (2017) Infiltration of M1, but not M2, macrophages is impaired after unilateral ureter obstruction in Nrf2-deficient mice.
    Sci Rep. 7 (1): 8801.
  36. Suzuki, Y. et al. (2017) Requisite role of vasohibin-2 in spontaneous gastric cancer formation and accumulation of cancer-associated fibroblasts.
    Cancer Sci. 108 (12): 2342-51.
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Our F4/80 (Cl:A3-1) Antibody has been referenced in >500 publications*


*Based on February 2018 data from CiteAb's antibody search engine.