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IL-10 antibody | CC318

Product Code Applications Pack Size List Price Your Price Qty
MCA2110
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E ES F * 0.5 mg loader
List Price Your Price
loader

Mouse anti Bovine interleukin-10 antibody, clone CC318 recognizes bovine IL-10. Mouse anti Bovine interleukin-10 antibody, clone CC318 has been shown not to inhibit the biological activity of IL-10.

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Horse
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
Plasmid cDNA encoding bovine IL-10.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 5ug/ml 10ug/ml
ELISpot
Flow Cytometry 1
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
Mouse anti Bovine interleukin-10 antibody, clone CC318 may be used as a capture antibody in a sandwich ELISA assay for bovine IL-10 in combination with MCA2111B as detection reagent (Bannermann et al. 2004).

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References for IL-10 antibody

  1. Kwong, L.S. et al. (2002) Development of an ELISA for bovine IL-10.
    Vet Immunol Immunopathol. 85 (3-4): 213-23.
  2. Bannerman, D.D. et al. (2004) Escherichia coli and Staphylococcus aureus elicit differential innate immune responses following intramammary infection.
    Clin Diagn Lab Immunol. 11: 463-72.
  3. Abbott, J.R. et al. (2005) Rapid and long-term disappearance of CD4+ T lymphocyte responses specific for Anaplasma marginale major surface protein-2 (MSP2) in MSP2 vaccinates following challenge with live A. marginale.
    J Immunol. 174: 6702-15.
  4. Berger, S.T. and Griffin, F.T. (2006) A comparison of ovine monocyte-derived macrophage function following infection with Mycobacterium avium ssp. avium and Mycobacterium avium ssp. paratuberculosis.
    Immunol Cell Biol. 84: 349-56.
  5. Davis, T.L. and Pate, J.L. (2007) Bovine luteal cells stimulate proliferation of major histocompatibility nonrestricted gamma delta T cells.
    Biol Reprod. 77: 914-22.
  6. Denis, M. et al. (2007) Enhancement of the sensitivity of the whole-blood gamma interferon assay for diagnosis of Mycobacterium bovis infections in cattle.
    Clin Vaccine Immunol. 14 (11): 1483-9.
  7. Hamza, E. et al. (2007) Modulation of allergy incidence in icelandic horses is associated with a change in IL-4-producing T cells.
    Int Arch Allergy Immunol. 144: 325-37.
  8. Souza, M. et al. (2008) Pathogenesis and immune responses in gnotobiotic calves after infection with the genogroup II.4-HS66 strain of human norovirus.
    J Virol. 82: 1777-86.
  9. View The Latest Product References
  10. Flynn, R.J. et al. (2008) Possible role for Toll-like receptors in interaction of Fasciola hepatica excretory/secretory products with bovine macrophages.
    Infect Immun. 76: 678-84.
  11. Weiss DJ et al. (2008) Bovine monocyte TLR2 receptors differentially regulate the intracellular fate of Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium.
    J Leukoc Biol. 83 (1): 48-55.
  12. Scandurra, G.M. et al. (2009) Assessment of live candidate vaccines for paratuberculosis in animal models and macrophages.
    Infect Immun. 78: 1383-9.
  13. Olivier, M. et al. (2009) Capacities of migrating CD1b+ lymph dendritic cells to present Salmonella antigens to naive T cells.
    PLoS One. 7: e30430.
  14. Jones, G.J. et al. (2010) Simultaneous measurement of antigen-stimulated interleukin-1 beta and gamma interferon production enhances test sensitivity for the detection of Mycobacterium bovis infection in cattle.
    Clin Vaccine Immunol. 17: 1946-51.
  15. Coad, M. et al. (2010) Repeat tuberculin skin testing leads to desensitisation in naturally infected tuberculous cattle which is associated with elevated interleukin-10 and decreased interleukin-1 beta responses.
    Vet Res. 41: 14.
  16. Ferret-Bernard, S. et al. (2010) Cellular and molecular mechanisms underlying the strong neonatal IL-12 response of lamb mesenteric lymph node cells to R-848.
    PLoS One. 5: e13705.
  17. Parker, D.G. et al. (2010) Lentivirus-mediated gene transfer of interleukin 10 to the ovine and human cornea.
    Clin Experiment Ophthalmol. 38: 405-13.
  18. Rinaldi, M. et al (2010) A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. coli mastitis.
    Funct Integr Genomics. 10: 21-38.
  19. Wenz, J.R. et al. (2010) Factors associated with concentrations of select cytokine and acute phase proteins in dairy cows with naturally occurring clinical mastitis.
    J Dairy Sci. 93: 2458-70.
  20. den Hartog, G. et al. (2011) Modulation of human immune responses by bovine interleukin-10.
    PLoSone 6: e18188
  21. Shu, D. et al. (2011) Diverse cytokine profile from mesenteric lymph node cells of cull cows severely affected with Johne's disease.
    Clin Vaccine Immunol. 18: 1467-76.
  22. Ferret-Bernard, S. et al. (2011) Mesenteric lymph node cells from neonates present a prominent IL-12 response to CpG oligodeoxynucleotide via an IL-15 feedback loop of amplification.
    Vet Res. 42:19.
  23. Ikebuchi, R. et al. (2013) Blockade of bovine PD-1 increases T cell function and inhibits bovine leukemia virus expression in B cells in vitro.
    Vet Res. 44: 59.
  24. McGill, J.L. et al. (2013) Differential chemokine and cytokine production by neonatal bovine γ/δ T-cell subsets in response to viral toll-like receptor agonists and in vivo respiratory syncytial virus infection.
    Immunology. 139: 227-44.
  25. Redondo, E. et al. (2014) Induction of interleukin-8 and interleukin-12 in neonatal ovine lung following experimental inoculation of bovine respiratory syncytial virus.
    J Comp Pathol. 150 (4): 434-48.
  26. Dooley LM et al. (2015) Effect of mesenchymal precursor cells on the systemic inflammatory response and endothelial dysfunction in an ovine model of collagen-induced arthritis.
    PLoS One. 10 (5): e0124144.
  27. Rainard, P. et al. (2016) Innate and Adaptive Immunity Synergize to Trigger Inflammation in the Mammary Gland.
    PLoS One. 11 (4): e0154172.
  28. Pomeroy B et al. (2016) Impact of in vitro treatments of physiological levels of estradiol and progesterone observed in pregnancy on bovine monocyte-derived dendritic cell differentiation and maturation.
    Vet Immunol Immunopathol. 182: 37-42.
  29. Rodrigues, V. et al. (2017) Development of a bead-based multiplexed assay for simultaneous quantification of five bovine cytokines by flow cytometry.
    Cytometry A. 91 (9): 901-7.
  30. Westover, A. et al. (2017) Effect of Human Amnion Epithelial Cells on the Acute Inflammatory Response in Fetal Sheep.
    Front Physiol. 8: 871.
  31. Canal AM et al. (2017) Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection.
    Res Vet Sci. 110: 34-39.
  32. Cassady-Cain, R.L. et al. (2017) Inhibition of Antigen-Specific and Nonspecific Stimulation of Bovine T and B Cells by Lymphostatin from Attaching and Effacing Escherichia coli.
    Infect Immun. 85 (2): e00845-16.
  33. Jimbo, S. et al. (2019) Natural and inducible regulatory B cells are widely distributed in ovine lymphoid tissues.
    Vet Immunol Immunopathol. 211: 44-8.
  34. Stabel, J.R. & Bannantine, J.P. (2019) Divergent Antigen-Specific Cellular Immune Responses during Asymptomatic Subclinical and Clinical States of Disease in Cows Naturally Infected with Mycobacterium avium. subsp. paratuberculosis.
    Infect Immun. 88(1):e00650-19.
  35. Ciliberti, M.G. et al. (2020) Nexus Between Immune Responses and Oxidative Stress: The Role of Dietary Hydrolyzed Lignin in ex vivo Bovine Peripheral Blood Mononuclear Cell Response.
    Front Vet Sci. 7: 9.
  36. Stabel, J.R. et al. (2021) Comparative cellular immune responses in calves after infection with Mycobacterium avium. subsp. paratuberculosis., M. avium. subsp. avium., M. kansasii. and M. bovis..
    Vet Immunol Immunopathol. 237: 110268.
  37. Davidson, J.O. et al. (2021) Window of opportunity for human amnion epithelial stem cells to attenuate astrogliosis after umbilical cord occlusion in preterm fetal sheep.
    Stem Cells Transl Med. 10 (3): 427-40.
  38. Ciliberti, M.G. et al. (2022) Green extraction of bioactive compounds from wine lees and their bio-responses on immune modulation using in vitro sheep model.
    J Dairy Sci. 105 (5): 4335-53.
  39. Santillo, A. et al. (2022) Feeding tannins to dairy cows in different seasons improves the oxidative status of blood plasma and the antioxidant capacity of cheese.
    J Dairy Sci. 105 (11): 8609-20.
  40. Bouroutzika, E. et al. (2023) Melatonin Administration to Pregnant Ewes for Coccidiosis Control in Their Offspring.
    Animals (Basel). 13 (14): 2381.
  41. de Silva. K. et al. (2018) Defining resilience to mycobacterial disease: Characteristics of survivors of ovine paratuberculosis.
    Vet Immunol Immunopathol. 195: 56-64.
  42. Stabel, J.R. et al. (2020) Comparison of Sheep, Goats, and Calves as Infection Models for Mycobacterium avium subsp. paratuberculosis.
    Vet Immunol Immunopathol. 225: 110060.
  43. Galinsky, R. et al. (2020) Tumor necrosis factor inhibition attenuates white matter gliosis after systemic inflammation in preterm fetal sheep.
    J Neuroinflammation. 17 (1): 92.
  44. Ciliberti, M.G. et al. (2024) Role of hazelnut skin supplementation on plasma antioxidant status and cytokine profile in growing lambs.
    Front Vet Sci. 11: 1340141.

ELISA

ELISpot

Flow Cytometry

RRID
AB_2249021
UniProt
P43480
Entrez Gene
IL10
GO Terms
GO:0002237 response to molecule of bacterial origin
GO:0002740 negative regulation of cytokine secretion involved in immune response
GO:0002904 positive regulation of B cell apoptosis
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0030889 negative regulation of B cell proliferation
GO:0032715 negative regulation of interleukin-6 production
GO:0032800 receptor biosynthetic process
GO:0042095 interferon-gamma biosynthetic process
GO:0043193 positive regulation of gene-specific transcription
GO:0045019 negative regulation of nitric oxide biosynthetic process
GO:0045077 negative regulation of interferon-gamma biosynthetic process
GO:0050715 positive regulation of cytokine secretion
GO:0051045 negative regulation of membrane protein ectodomain proteolysis
GO:0051091 positive regulation of transcription factor activity
GO:0051384 response to glucocorticoid stimulus

MCA2110

147808 166550

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