GM-CSF Antibody | 8D8

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  • Mouse anti Sheep GM-CSF
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  • Product Type
    Monoclonal Antibody
  • Clone
    8D8
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1923E, WBdatasheet pdfdatasheet pdf0.25 mg
    MCA1923
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Sheep GM-CSF antibody, clone 8D8 recognizes both recombinant and native ovine granulocyte / macrophage colony-stimulating factor (GM-CSF), also known as Colony stimulating factor (CSF).

      GM-CSF is one of several cytokines that regulate the survival, growth and differentiation of haemopoietic cells. GM-CSF is secreted from a range of cells including macrophages, T-cells, endothelial cells and fibroblasts in response to antigen, LPS or mitogen stimulation.

      Mouse anti Sheep GM-CSF antibody, clone 8D8 may be used as a capture antibody in ELISA assays with biotinylated Mouse anti Sheep GM-CSF antibody, (clone 3C2 ) as a detection reagent for the detection of ovine GM-CSF.

      Clone 8D8 has been reported to neutralize the activity of both recombinant and native ovine GM-CSF (Entrican et al. 1996). Removal of the preservative is recommended prior to use in functional assays - Bio-Rad recommend the use of a Slide-A-Lyser® dialysis cassette for this purpose.
    • Intended Use
    • Target Species
      Sheep
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Bovineno
      HorseWeak
      GoatWeak
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      CHO expressed recombinant ovine GM-CSF.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the NS0 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      cytokine activity
      growth factor activity
      immune response
      granulocyte macrophage colony-stimulating factor receptor binding
      extracellular space
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This reagent was evaluated at the Moredun Research Institute and The Roslin Institute at the University of Edinburgh as part of ‘The route to identification of immunological correlates of protection in ruminants’ Industrial Partnership Award funded by BBSRC (grant numbers BB/I019863/1; BB/I020519/1) with the support of Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA5ug/ml
      Western Blotting

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional GM-CSF Antibody Formats

    Formats Clone Applications Sizes available
    GM-CSF Antibody : Purified 8D8 E, WB 0.25 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit F(ab')2 anti Mouse IgG:BiotinSTAR11B1 mgC, E, F, P
      STAR11B

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                ELISA

              References

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