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IgG2a antibody | MARG2a-1

Mouse anti Rat IgG2a Heavy Chain

Product Type
Monoclonal Antibody
Clone
MARG2a-1
Isotype
IgG1
Specificity
IgG2a
Region
HEAVY CHAIN

Product Code Applications Pack Size List Price Your Price Qty
MCA278
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.25 mg loader
List Price Your Price
loader

Mouse anti Rat IgG2a heavy chain antibody, clone MARG2a-1 recognizes the rat gamma 2a immunoglobulin heavy chain, and does not cross-react with other immunoglobulin classes or subclasses.

Avidity of Mouse anti Rat IgG2a heavy chain antibody, clone MARG2a-1 for rat IgG2a = 2.1 x 109 M-1.

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide
Immunogen
Purified rat IgG2a.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the PAI-0 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.25ug/ml as coating antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
ELISA
This product may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA together with MCA1296P as detection reagent.

Purified rat IgA (PRP04) may be used as a standard.

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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
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References for IgG2a antibody

  1. Bazin, H. et al. (1973) Brief communication: Transplantable immunoglobulin-secreting tumors in rats. V. Monoclonal immunoglobulins secreted by 250 ileocecal immunocytomas in LOU-Wsl rats.
    J Natl Cancer Inst. 51 (4): 1359-61.
  2. Bazin, H. et al. (1974) Three classes and four (sub)classes of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c.
    Eur J Immunol. 4 (1): 44-8.
  3. Sandin, L.C. et al. (2014) Locally delivered CD40 agonist antibody accumulates in secondary lymphoid organs and eradicates experimental disseminated bladder cancer.
    Cancer Immunol Res. 2 (1): 80-90.
  4. Bézie, S. et al. (2015) Fibrinogen-like protein 2/fibroleukin induces long-term allograft survival in a rat model through regulatory B cells.
    PLoS One. 10 (3): e0119686.
  5. Bézie S et al. (2015) Compensatory Regulatory Networks between CD8 T, B, and Myeloid Cells in Organ Transplantation Tolerance.
    J Immunol. 195 (12): 5805-15.
  6. Ueta, H. et al. (2018) Single blood transfusion induces the production of donor-specific alloantibodies and regulatory T cells mainly in the spleen.
    Int Immunol. 30 (2): 53-67.
  7. Huizinga, R. et al. (2012) Sialylation of Campylobacter jejuni lipo-oligosaccharides: impact on phagocytosis and cytokine production in mice.
    PLoS One. 7 (3): e34416.

Further Reading

  1. Köhler, G. & Milstein, C. (1975) Continuous cultures of fused cells secreting antibody of predefined specificity.
    Nature. 256 (5517): 495-7.
  2. Bazin, H. et al. (1978) Transplantable IgD immunoglobulin-secreting tumors in rat.
    J. Immunol. 121: 2077-2082.
  3. Shulman, M. et al. (1978) A better cell line for making hybridomas secreting specific antibodies.
    Nature. 276 (5685): 269-70.

RRID
AB_322914
UniProt
P20760
Entrez Gene
LOC679045
GO Terms
GO:0003823 antigen binding

MCA278

147619 155847 161147 167304

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