Kappa/Lambda Light Chain Antibody | MARK-1/MARL-15

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  • Mouse anti Rat Kappa/Lambda Light Chain:HRP
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    MARK-1/MARL-15
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1296PEdatasheet pdfdatasheet pdf0.5 mg
    MCA1296P
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Rat Kappa/Lambda Light Chain: antibody cocktail is a mixture of clones MARK-1 and MARL-15 recognizing rat kappa and lambda light chains respectively. The cocktail doe not recognise immunogloulin heavy chsims.
    • Intended Use
    • Target Species
      Rat
    • Product Form
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography from tissue culture supernatant
    • Preservative Stabilisers
      50%Glycerol
      0.01%Thiomersal
    • Immunogen
      MARK-1: Rat kappa light chains (IR202 and IR968) MARL-15: Rat lambda light chains (RH58, IR31)
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immuniszed BALB/c mice were fused with cells of the SP2/0 (MARK-1) or PAI-O (MARL-15) mouse myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.5ug/ml as detecting antibody

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      HRP-conjugated MARK-1/MARL-15 clone cocktail may be used as a detection reagent for rat immunoglobulins in ELISA assays and can be used in combination with coating antibodies:

      rat IgG1MCA194
      rat IgG2aMCA278
      rat IgG2bMCA1294
      rat IgG2cMCA1295
      rat IgMMCA189
      rat IgEMCA193
      rat IgAMCA191
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Kappa/Lambda Light Chain Antibody Formats

    Formats Clone Applications Sizes available
    Kappa/Lambda Light Chain Antibody : HRP MARK-1/MARL-15 E 0.5 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          AbGUARD® HRP Stabilizer PlusBUF052A100 mlC, E, P, WB
          BUF052A
          TMB Signal+BUF054A100 mlE
          BUF054A
          TMB CoreBUF056A100 mlE
          BUF056A
          TMB Core PrestainedBUF057A100 mlE
          BUF057A
          TMB Core+BUF062A100 mlE
          BUF062A
          AbGUARD® HRP Stabilizer PlusBUF052C1000 mlC, E, P, WB
          BUF052C
          AbGUARD® HRP Stabilizer PlusBUF052B500 mlC, E, P, WB
          BUF052B

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                ELISA

              References

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