GM-CSF antibody | 3C2

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Mouse anti Sheep GM-CSF

Product Type
Monoclonal Antibody
Clone
3C2
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1924 E WB 0.5 mg
Mouse anti Sheep GM-CSF, clone 3C2 recognizes both recombinant and native ovine Granulocyte-macrophage colony-stimulating factor, also known GM-CSF or CSF, a 16.3kDa cytokine responsible for the stimulation and growth of cells from granulocyte, macrophage, eosinophil and erythrocyte hematopoietic precursor cell linages.

Mouse anti Sheep GM-CSF, clone 3C2 has is of particular interest as it has been shown to reconize GM-CSF in cattle while other clones such as 8D8 do not crosss react with cattle..

Mouse anti Sheep GM-CSF, clone 3C2 has been reported to neutralise the activity of both recombinant ovine and native GM-CSF.

Removal of sodium azide is recommended prior to use in functional assays. Bio-Rad recommend the use of EQU003 for this purpose.

Product Details

Target Species
Sheep
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
CHO expressed recombinant ovine GM-CSF.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the NSO myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P28773 Related reagents
Entrez Gene
CSF2 Related reagents
GO Terms
GO:0005125 cytokine activity
GO:0005129 granulocyte macrophage colony-stimulating factor receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
Acknowledgements
This reagent was evaluated at the Moredun Research Institute and The Roslin Institute at the University of Edinburgh as part of ‘The route to identification of immunological correlates of protection in ruminants’ Industrial Partnership Award funded by BBSRC (grant numbers BB/I019863/1; BB/I020519/1) with the support of Bio-Rad.
Regulatory
For research purposes only

Applications of GM-CSF antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
Mouse anti Sheep GM-CSF antibody, clone 3C2 (MCA1924 can be used in a sandwich ELISA as a capture antibody together with Mouse anti Sheep GM-CSF antibody, clone 8D8 (MCA1923) as the detection antibody. Conjugation of the detection antibody with LYNX Rapid or Rapid Plus Kits is recommended.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images

ELISA

Product Specific References

References for GM-CSF antibody

  1. Entrican, G. et al. (1996) Development of a sandwich ELISA for ovine granulocyte/macrophage colony-stimulating factor.
    Vet Immunol Immunopathol. 50 (1-2): 105-15.
  2. Deane, D. et al. (2000) Orf virus encodes a novel secreted protein inhibitor of granulocyte-macrophage colony-stimulating factor and interleukin-2.
    J Virol. 74: 1313-20.
  3. Foulon, E. and Foucras, G. (2008) Two populations of ovine bone marrow-derived dendritic cells can be generated with recombinant GM-CSF and separated on CD11b expression.
    J Immunol Methods. 339: 1-10.
  4. Haig, D.M. et al. (1995) Haemopoietic cell responses in the blood and bone marrow of sheep infected with the abomasal nematode Telodorsagia circumcincta.
    J Comp Pathol. 112: 151-64.
  5. Scott, J.L. (2009) Spermatozoa and seminal plasma induce a greater inflammatory response in the ovine uterus at oestrus than dioestrus.
    Reprod Fertil Dev. 21: 817-26.
  6. John, H.A. et al. (1994) Generation of an ovine bone marrow-derived myelomonocyte-like cell line by retroviral-mediated transformation. Immunological characterization and the effect of cytokines and lipopolysaccharides.
    J Leukoc Biol. 55: 785-92.
  7. Kennedy, H.E. et al. (2002) Modulation of immune responses to Mycobacterium bovis in cattle depleted of WC1(+) gamma delta T cells.
    Infect Immun. 70: 1488-500.
  8. McInnes, C.J. et al. (2005) Glycosylation, disulfide bond formation, and the presence of a WSXWS-like motif in the orf virus GIF protein are critical for maintaining the integrity of Binding to ovine granulocyte-macrophage colony-stimulating factor and interleukin-2.
    J Virol. 79: 11205-13.
  9. Schnabel, C.L. et al. (2013) Evaluation of the reactivity of commercially available monoclonal antibodies with equine cytokines
    Veterinary Immunology and Immunopathology. 156 (1-2): 1-19.