IgG2a antibody | MARG2a-1

Mouse anti Rat IgG2a Heavy Chain:Biotin
Mouse anti Rat IgG2a Heavy Chain:FITC
Mouse anti Rat IgG2a Heavy Chain:HRP
Mouse anti Rat IgG2a Heavy Chain
- Product Type
- Monoclonal Antibody
- Clone
- MARG2a-1
- Isotype
- IgG1
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
MCA278B | E | 0.5 mg |
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MCA278F | F | 0.5 mg |
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MCA278P | E | 0.5 mg |
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MCA278 | E | 0.25 mg |
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Avidity of Mouse anti Rat IgG2a heavy chain antibody, clone MARG2a-1 for rat IgG2a = 2.1 x 109 M-1.
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide 50% Glycerol - Preservative Stabilisers
0.1% Sodium Azide 50% Glycerol - Preservative Stabilisers
0.01% Thiomersal 50% Glycerol - Preservative Stabilisers
0.1% Sodium Azide - Immunogen
- purified rat IgG2a.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the PAI-0 mouse myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P20760
- Entrez Gene
- LOC679045
- GO Terms
- GO:0003823 antigen binding
- Regulatory
- For research purposes only
Applications of IgG2a antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 500ng/ml | ||
Flow Cytometry | 5ug/ml | ||
ELISA | 0.5ug/ml | ||
ELISA | 1.25ug/ml as coating antibody |
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG1 Negative Control:FITC | MCA1209F | F | 0.1 mg |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | ||
TMB Signal+ | BUF054A | E | 100 ml | ||
TMB Core | BUF056A | E | 100 ml | ||
TMB Core+ | BUF062A | E | 100 ml |
Product Specific References
References for IgG2a antibody
-
Bazin, H. et al. (1974) Three classes and four (sub)classes of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c.
Eur J Immunol. 4 (1): 44-8. -
Shulman, M. et al. (1978) A better cell line for making hybridomas secreting specific antibodies.
Nature. 276 (5685): 269-70. -
Bazin, H. et al. (1973) Brief communication: Transplantable immunoglobulin-secreting tumors in rats. V. Monoclonal immunoglobulins secreted by 250 ileocecal immunocytomas in LOU-Wsl rats.
J Natl Cancer Inst. 51 (4): 1359-61. -
Sandin, L.C. et al. (2014) Locally delivered CD40 agonist antibody accumulates in secondary lymphoid organs and eradicates experimental disseminated bladder cancer.
Cancer Immunol Res. 2 (1): 80-90. -
Bézie S et al. (2015) Compensatory Regulatory Networks between CD8 T, B, and Myeloid Cells in Organ Transplantation Tolerance.
J Immunol. 195 (12): 5805-15. -
Bézie, S. et al. (2015) Fibrinogen-like protein 2/fibroleukin induces long-term allograft survival in a rat model through regulatory B cells.
PLoS One. 10 (3): e0119686.
Further Reading
-
Köhler, G. & Milstein, C. (1975) Continuous cultures of fused cells secreting antibody of predefined specificity.
Nature. 256 (5517): 495-7. -
Bazin, H. et al. (1978) Transplantable IgD immunoglobulin-secreting tumors in rat.
J. Immunol. 121: 2077-2082.
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