IgG2a antibody | MARG2a-1
Biotinylated Mouse anti Rat IgG2a antibody, clone Marg2a-1 (MCA278B) used to label bound anti CD169 antibody by flow cytometry.
Image caption:
Sialoadhesin is expressed on BM-Mø and is upregulated upon C. jejuni LOS stimulation. Cells were stimulated overnight with GB11 wt or Cst-II mutant LOS and sialoadhesin expression was examined by flow cytometry. Sialoadhesin was expressed on unstimulated BM-Mø (A; dotted line) and was upregulated after stimulation with GB11 LOS (B, and solid line in A). Filled histograms represent background fluorescence when incubated with the secondary antibody alone. BM-DC only express low levels of sialoadhesin and do not upregulate sialoadhesin in response to GB11 LOS (C).
From: Huizinga R, Easton AS, Donachie AM, Guthrie J, van Rijs W, Heikema A, Boon L, Samsom JN, Jacobs BC, Willison HJ, Goodyear CS.
Sialylation of Campylobacter jejuni lipo-oligosaccharides: impact on phagocytosis and cytokine production in mice.
PLoS One. 2012;7(3):e34416.
doi: 10.1371/journal.pone.0034416.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat IgG2a Heavy Chain
- Product Type
- Monoclonal Antibody
- Clone
- MARG2a-1
- Isotype
- IgG1
- Specificity
- IgG2a
- Region
- HEAVY CHAIN
Antibody Quality and Performance Guarantee
| Mouse anti Rat IgG2a heavy chain antibody, clone MARG2a-1 recognizes the rat gamma 2a immunoglobulin heavy chain, and does not cross-react with other immunoglobulin classes or subclasses. Avidity of Mouse anti Rat IgG2a heavy chain antibody, clone MARG2a-1 for rat IgG2a = 2.1 x 109 M-1. |
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide - Immunogen
- Purified rat IgG2a.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of the PAI-0 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1.25ug/ml as coating antibody |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
References for IgG2a antibody
-
Bazin, H. et al. (1973) Brief communication: Transplantable immunoglobulin-secreting tumors in rats. V. Monoclonal immunoglobulins secreted by 250 ileocecal immunocytomas in LOU-Wsl rats.
J Natl Cancer Inst. 51 (4): 1359-61. -
Bazin, H. et al. (1974) Three classes and four (sub)classes of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c.
Eur J Immunol. 4 (1): 44-8. -
Sandin, L.C. et al. (2014) Locally delivered CD40 agonist antibody accumulates in secondary lymphoid organs and eradicates experimental disseminated bladder cancer.
Cancer Immunol Res. 2 (1): 80-90. -
Bézie, S. et al. (2015) Fibrinogen-like protein 2/fibroleukin induces long-term allograft survival in a rat model through regulatory B cells.
PLoS One. 10 (3): e0119686. -
Bézie S et al. (2015) Compensatory Regulatory Networks between CD8 T, B, and Myeloid Cells in Organ Transplantation Tolerance.
J Immunol. 195 (12): 5805-15. -
Ueta, H. et al. (2018) Single blood transfusion induces the production of donor-specific alloantibodies and regulatory T cells mainly in the spleen.
Int Immunol. 30 (2): 53-67. -
Huizinga, R. et al. (2012) Sialylation of Campylobacter jejuni lipo-oligosaccharides: impact on phagocytosis and cytokine production in mice.
PLoS One. 7 (3): e34416.
Further Reading
-
Köhler, G. & Milstein, C. (1975) Continuous cultures of fused cells secreting antibody of predefined specificity.
Nature. 256 (5517): 495-7. -
Bazin, H. et al. (1978) Transplantable IgD immunoglobulin-secreting tumors in rat.
J. Immunol. 121: 2077-2082. -
Shulman, M. et al. (1978) A better cell line for making hybridomas secreting specific antibodies.
Nature. 276 (5685): 269-70.
- RRID
- AB_322914
- UniProt
- P20760
- Entrez Gene
- LOC679045
- GO Terms
- GO:0003823 antigen binding
MCA278
147619
155847
161147
167304
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