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IgG2a antibody | MARG2a-1

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Mouse anti Rat IgG2a Heavy Chain:Biotin

Mouse anti Rat IgG2a Heavy Chain:FITC

Mouse anti Rat IgG2a Heavy Chain:HRP

Mouse anti Rat IgG2a Heavy Chain

Product Type
Monoclonal Antibody
Clone
MARG2a-1
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA278B
Datasheet
E
MSDS
0.5 mg loader

MCA278F
Datasheet
F
MSDS
0.5 mg loader

MCA278P
Datasheet
E
MSDS
0.5 mg loader

MCA278
Datasheet
E
MSDS
0.25 mg loader

Mouse anti Rat IgG2a heavy chain antibody, clone MARG2a-1 recognizes the rat gamma 2a immunoglobulin heavy chain, and does not cross-react with other immunoglobulin classes or subclasses.

Avidity of Mouse anti Rat IgG2a heavy chain antibody, clone MARG2a-1 for rat IgG2a = 2.1 x 109 M-1.

Product Details

Target Species
Rat
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide
50%Glycerol
Preservative Stabilisers
0.1%Sodium Azide
50%Glycerol
Preservative Stabilisers
0.01%Thiomersal
50%Glycerol
Preservative Stabilisers
0.1%Sodium Azide
Immunogen
purified rat IgG2a.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the PAI-0 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P20760
Entrez Gene
LOC679045
GO Terms
GO:0003823 antigen binding
Regulatory
For research purposes only

Applications of IgG2a antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 500ng/ml
Flow Cytometry 5ug/ml
ELISA 0.5ug/ml
ELISA 1.25ug/ml as coating antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
ELISA
This product may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA together with MCA1296P as detection reagent.

Purified rat IgA (PRP04) may be used as a standard.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control:FITC MCA1209F F 0.1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
TMB Signal+ BUF054A E 100 ml
TMB Core BUF056A E 100 ml
TMB Core+ BUF062A E 100 ml

Product Specific References

References for IgG2a antibody

  1. Bazin, H. et al. (1974) Three classes and four (sub)classes of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c.
    Eur J Immunol. 4 (1): 44-8.
  2. Shulman, M. et al. (1978) A better cell line for making hybridomas secreting specific antibodies.
    Nature. 276 (5685): 269-70.
  3. Bazin, H. et al. (1973) Brief communication: Transplantable immunoglobulin-secreting tumors in rats. V. Monoclonal immunoglobulins secreted by 250 ileocecal immunocytomas in LOU-Wsl rats.
    J Natl Cancer Inst. 51 (4): 1359-61.
  4. Sandin, L.C. et al. (2014) Locally delivered CD40 agonist antibody accumulates in secondary lymphoid organs and eradicates experimental disseminated bladder cancer.
    Cancer Immunol Res. 2 (1): 80-90.
  5. Bézie S et al. (2015) Compensatory Regulatory Networks between CD8 T, B, and Myeloid Cells in Organ Transplantation Tolerance.
    J Immunol. 195 (12): 5805-15.
  6. Bézie, S. et al. (2015) Fibrinogen-like protein 2/fibroleukin induces long-term allograft survival in a rat model through regulatory B cells.
    PLoS One. 10 (3): e0119686.

Further Reading

  1. Köhler, G. & Milstein, C. (1975) Continuous cultures of fused cells secreting antibody of predefined specificity.
    Nature. 256 (5517): 495-7.
  2. Bazin, H. et al. (1978) Transplantable IgD immunoglobulin-secreting tumors in rat.
    J. Immunol. 121: 2077-2082.
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