IgG1 antibody | MARG1-2
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Mouse anti Rat IgG1 Heavy Chain
- Product Type
- Monoclonal Antibody
- Clone
- MARG1-2
- Isotype
- IgG1
- Specificity
- IgG1
- Region
- HEAVY CHAIN
Antibody Quality and Performance Guarantee
| Mouse anti rat IgG1, clone MARG1-2 recognizes the heavy chain of the rat immunoglobulin G1 subclass. No cross-reactivity has been found with other rat immunoglobulin classes or subclasses. |
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide - Immunogen
- Rat IR27 IgG1 myeloma protein
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
3ug/ml | 5ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- ELISA
- Clone MARG1-2 may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with a HRP-conjugated MARK-1/MARL-15 clone cocktail as the detection reagent.
References for IgG1 antibody
-
Pelegrí, C. et al. (2001) Prevention of adjuvant arthritis by the W3/25 anti-CD4 monoclonal antibody is associated with a decrease of blood CD4(+)CD45RC(high) T cells.
Clin Exp Immunol. 125 (3): 470-7. -
Sato, K. et al. (2001) Carbon monoxide generated by heme oxygenase-1 suppresses the rejection of mouse-to-rat cardiac transplants.
J Immunol. 166 (6): 4185-94. -
Bézie, S. et al. (2015) Fibrinogen-Like Protein 2/Fibroleukin Induces Long-Term Allograft Survival in a Rat Model through Regulatory B Cells.
PLoS One. 10 (3): e0119686. -
Bézie, S. et al. (2015) Compensatory Regulatory Networks between CD8 T, B, and Myeloid Cells in Organ Transplantation Tolerance.
J Immunol. 195 (12): 5805-15. -
Ueta, H. et al. (2018) Single blood transfusion induces the production of donor-specific alloantibodies and regulatory T cells mainly in the spleen
International Immunology. 30 (2): 53-67.
- RRID
- AB_321807
- UniProt
- P20759
- Entrez Gene
- Ighg
- GO Terms
- GO:0003823 antigen binding
MCA194
159665
180227
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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