CD4 antibody | OX-68

Mouse anti Rat CD4 (Domains 3 and 4)

Product Type
Monoclonal Antibody
Clone
OX-68
Isotype
IgG2a
Specificity
CD4
Region
(DOMAINS 3 AND 4)

Product Code Applications Pack Size List Price Your Price Qty
MCA1022R
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.25 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Rat CD4 (Domains 3 and 4) monoclonal antibody, clone OX-68 recognizes domains 3 and 4 of rat CD4, a single pass type I membrane glycoprotein.

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the NS1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)

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Description Mouse IgG2a Negative Control

References for CD4 antibody

  1. Kerr, J.S, and Wright, G.J. (2012) Avidity-based extracellular interaction screening (AVEXIS) for the scalable detection of low-affinity extracellular receptor-ligand interactions.
    J Vis Exp. 5(61): e3881.
  2. Crosnier, C. et al. (2013) A library of functional recombinant cell-surface and secreted P. falciparum merozoite proteins.
    Mol Cell Proteomics. 12 (12): 3976-86.
  3. Crosnier, C. et al. (2016) Binding of Plasmodium falciparum Merozoite Surface Proteins DBLMSP and DBLMSP2 to Human Immunoglobulin M Is Conserved among Broadly Diverged Sequence Variants.
    J Biol Chem. 291 (27): 14285-99.
  4. Liu, Y. et al. (2018) Structural Basis for Draxin-Modulated Axon Guidance and Fasciculation by Netrin-1 through DCC.
    Neuron. 97 (6): 1261-1267.e4.
  5. Sharma, S. & Wright, G.J. (2020) Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens.
    J Vis Exp. 2020 Jun 06 (160).
  6. van Wilgenburg, B. et al. (2014) The productive entry pathway of HIV-1 in macrophages is dependent on endocytosis through lipid rafts containing CD4.
    PLoS One. 9 (1): e86071.
  7. Proto, W.R. et al. (2019) Adaptation of Plasmodium falciparum. to humans involved the loss of an ape-specific erythrocyte invasion ligand.
    Nat Commun. 10 (1): 4512.

RRID
AB_567282
UniProt
P05540
Entrez Gene
Cd4
GO Terms
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0006955 immune response
GO:0019901 protein kinase binding
GO:0045058 T cell selection
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Rat Products