Mouse anti Rat CD4 (Domains 3 and 4) monoclonal antibody, clone OX-68 recognizes domains 3 and 4 of rat CD4, a single pass type I membrane glycoprotein.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Carrier Free
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the NS1 myeloma cell line.
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- Entrez Gene
- GO Terms
integral to membrane
protein kinase binding
T cell selection
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD4 antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
Product Specific References
References for CD4 antibody
McKnight, A.J. & Classon, B.J. (1992) Biochemical and immunological properties of rat recombinant interleukin-2 and interleukin-4.
Immunology. 75 (2): 286-92.
Kerr, J.S, and Wright, G.J. (2012) Avidity-based extracellular interaction screening (AVEXIS) for the scalable detection of low-affinity extracellular receptor-ligand interactions.
J Vis Exp. 5(61): e3881.
Crosnier, C. et al. (2013) A library of functional recombinant cell-surface and secreted P. falciparum merozoite proteins.
Mol Cell Proteomics. 12 (12): 3976-86.
Crosnier, C. et al. (2016) Binding of Plasmodium falciparum Merozoite Surface Proteins DBLMSP and DBLMSP2 to Human Immunoglobulin M Is Conserved among Broadly Diverged Sequence Variants.
J Biol Chem. 291 (27): 14285-99.