CD4 antibody | OX-68
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Mouse anti Rat CD4 (Domains 3 and 4) monoclonal antibody, clone OX-68 recognizes domains 3 and 4 of rat CD4, a single pass type I membrane glycoprotein.
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the NS1 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P05540 Related reagents
- Entrez Gene
- Cd4 Related reagents
- GO Terms
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0006955 immune response
- GO:0019901 protein kinase binding
- GO:0045058 T cell selection
- Regulatory
- For research purposes only
Applications of CD4 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/50 | 1/100 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA1210 | 100 Tests | F |
Product Specific References
References for CD4 antibody
-
McKnight, A.J. & Classon, B.J. (1992) Biochemical and immunological properties of rat recombinant interleukin-2 and interleukin-4.
Immunology. 75 (2): 286-92. -
Kerr, J.S, and Wright, G.J. (2012) Avidity-based extracellular interaction screening (AVEXIS) for the scalable detection of low-affinity extracellular receptor-ligand interactions.
J Vis Exp. 5(61): e3881. -
Crosnier, C. et al. (2013) A library of functional recombinant cell-surface and secreted P. falciparum merozoite proteins.
Mol Cell Proteomics. 12 (12): 3976-86. -
Crosnier C et al. (2016) Binding of Plasmodium falciparum merozoite surface proteins DBLMSP and DBLMSP2 to human immunoglobulin M is conserved amongst broadly diverged sequence variants.
J Biol Chem. May 12. pii: jbc.M116.722074. [Epub ahead of print]
