CD4 antibody | OX-68
Mouse anti Rat CD4 antiboy, clone OX-68 (MCA1022R) used for the developments of an ELISA curve detailing procedures to describe biotin- streptavidin ratios. Protocol is described here.
Image caption:
Establishing the ratios of biotinylated protein to streptavidin-PE using an ELISA-based method. An example of streptavidin-PE conjugation strategy used to generate an avid probe from a biotinylated monomeric protein. A dilution series of biotinylated monomers was incubated against a fixed concentration of streptavidin. The minimum dilution at which no excess biotinylated monomers can be detected was determined by ELISA. ELISA was performed with or without preincubating a range of protein dilutions with 10 ng of streptavidin-PE. In the presence of streptavidin-PE, the minimum dilution at which no signal was identified (circled black) and the amount of protein required for the saturation was calculated to generate a 10x stock solution with 4 μg/mL streptavidin-PE.
From: Sharma S, Wright GJ.
Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens.
J Vis Exp. 2020 Jun 6;(160).
doi: 10.3791/60803.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD4 antibody, clone CD-68 (MCA1022R) used to evaluate CD4 expression by flow cytometry.
Image caption:
Surface expression of chimeric CD4 in PSC-macrophages. A) PSC-macrophages were tested for surface CD4 expression by flow cytometry using three different clones of anti-CD4 antibodies. Representative histogram showing CD4 surface staining with mAb clone 11830 (red/brown line, recognises both endogenous human and chimeric CD4) is shown in Figure 6E. Staining with mAb clone OKT4 (blue line, left panel, recognises endogenous human CD4 only); or with mAb clone OX-68 (orange line, right panel, recognises chimeric CD4 only). All lines are depicted compared to the matched isotype control (shaded gray). Representation of the binding site of the antibodies on human and chimeric CD4 are shown at the bottom. B) Quantification of endogenous human CD4 expression relative to the PSC-macrophages control group, using mAb OKT4 (n = 4). C) Quantification of chimeric CD4 relative to the PSC-macrophages CD4WT group, using mAB OX68 (n = 6).
From: van Wilgenburg B, Moore MD, James WS, Cowley SA (2014)
The Productive Entry Pathway of HIV-1 in Macrophages Is Dependent on Endocytosis through Lipid Rafts Containing CD4.
PLoS ONE 9(1): e86071.
doi.10.1371/journal.pone.0086071
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD4 (Domains 3 and 4) monoclonal antibody, clone OX-68 recognizes domains 3 and 4 of rat CD4, a single pass type I membrane glycoprotein.
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the NS1 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P05540
- Entrez Gene
- Cd4
- GO Terms
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0006955 immune response
- GO:0019901 protein kinase binding
- GO:0045058 T cell selection
- Regulatory
- For research purposes only
Applications of CD4 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/50 | 1/100 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA1210 | F | 100 Tests |
|
Product Specific References
References for CD4 antibody
-
Kerr, J.S, and Wright, G.J. (2012) Avidity-based extracellular interaction screening (AVEXIS) for the scalable detection of low-affinity extracellular receptor-ligand interactions.
J Vis Exp. 5(61): e3881. -
Crosnier, C. et al. (2013) A library of functional recombinant cell-surface and secreted P. falciparum merozoite proteins.
Mol Cell Proteomics. 12 (12): 3976-86. -
Crosnier, C. et al. (2016) Binding of Plasmodium falciparum Merozoite Surface Proteins DBLMSP and DBLMSP2 to Human Immunoglobulin M Is Conserved among Broadly Diverged Sequence Variants.
J Biol Chem. 291 (27): 14285-99. -
Liu, Y. et al. (2018) Structural Basis for Draxin-Modulated Axon Guidance and Fasciculation by Netrin-1 through DCC.
Neuron. 97 (6): 1261-1267.e4. -
Sharma, S. & Wright, G.J. (2020) Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens.
J Vis Exp. 2020 Jun 06 (160). -
van Wilgenburg, B. et al. (2014) The productive entry pathway of HIV-1 in macrophages is dependent on endocytosis through lipid rafts containing CD4.
PLoS One. 9 (1): e86071.
Fluorescent Spectraviewer
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