CD172a antibody | OX-41

Mouse anti Rat CD172a

Product Type
Monoclonal Antibody
Clone
OX-41
Isotype
IgG2a
Specificity
CD172a

Product Code Applications Pack Size List Price Your Price Qty
MCA274GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F P * 0.1 mg loader
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loader
MCA274R
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F P * WB 0.25 mg loader
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loader
MCA274G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F P * WB 1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Rat CD172a antibody, clone OX-41 recognizes rat CD172a, also known as Signal regulatory protein (SIRP). CD172a is expressed selectively by myeloid cells and by neurons.

MRC OX-41 binds to a different epitope on SIRP than ED9. Mouse anti Rat CD172a antibody, clone OX-41 does not completely block the interaction of CD172a - CD47 (Vernon-Wilson et al. 2000).

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
MCA274GA, MCA274R: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
MCA274G: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Rat peritoneal macrophages.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with the NS0/U mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/100 Pack Size: 0.1 mg, 1 mg
1/10 Pack Size: 0.25 mg
1/200 Pack Size: 0.1 mg, 1 mg
1/50 Pack Size: 0.25 mg
Immunohistology - Frozen
Immunohistology - Paraffin 1
Western Blotting
  1. 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
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Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
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Description Goat anti Mouse IgG (Fc):HRP
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG2a Negative Control MCA1210 F 100 Tests loader
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Description Mouse IgG2a Negative Control

References for CD172a antibody

  1. Robinson, A.P. et al. (1986) Macrophage heterogeneity in the rat as delineated by two monoclonal antibodies MRC OX-41 and MRC OX-42, the latter recognizing complement receptor type 3.
    Immunology. 57 (2): 239-47.
  2. Barclay, A.N. (1981) The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues.
    Immunology. 42 (4): 593-600.
  3. Adams, S. et al. (1998) Signal-regulatory protein is selectively expressed by myeloid and neuronal cells.
    J Immunol. 161 (4): 1853-9.
  4. Santos, L.L. et al. (1997) Anti-neutrophil monoclonal antibody therapy inhibits the development of adjuvant arthritis.
    Clin Exp Immunol. 107: 248-53.
  5. Milling, S.W. et al. (2006) Collection of lymph-borne dendritic cells in the rat.
    Nat Protoc. 1: 2263-70.
  6. Moghaddami, M. et al. (2007) Recruitment of dendritic cells and macrophages during T cell-mediated synovial inflammation.
    Arthritis Res Ther. 9: R120.
  7. Zhou, Y.J. et al. (2010) The role of the lactadherin in promoting intestinal DCs development in vivo and invitro.
    Clin Dev Immunol. 2010: 357541.
  8. Varalakshmi, Ch, et al. (2008) Immunomodulatory effects of curcumin: in-vivo.
    Int Immunopharmacol. 8 (5): 688-700.
  9. View The Latest Product References

Synonyms
SIRP ALPHA
RRID
AB_323480
UniProt
P97710
Entrez Gene
Sirpa
GO Terms
GO:0016477 cell migration
GO:0017124 SH3 domain binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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