CD172a antibody | OX-41
Mouse anti Rat CD172a antibody, clone OX-41(MCA274GA) used for the flow cytometric analysis of Peyer's patch cells.
Image caption:
Flow cytometric analyses of CD3+CD4+CD25+T-cells in PPs of each group in different development stages (Mean ± SD). In order to investigate T cells proliferation and differentiation in three differently fed groups, we examined the phenotype of CD3+CD4+T cells subpopulation in the PPs by assessing the relative proportions of CD3+CD4+CD25+T cells. The results showed that the group of LR rats had a higher relative proportion of CD3+CD4+CD25+ T cells in PPs (b A: BR at W3, B: AR at W3, C: LR at W3, D: BR at W5, E: AR at W5, F: LR at W5, G: BR at W7, H: AR at W7, I: LR at W7, J: BR at W11, K:AR at W11, L: LR at W11). *P<.05.
From: Zhou YJ, Gao J, Yang HM, Yuan XL, Chen TX, He ZJ.
The role of the lactadherin in promoting intestinal DCs development in vivo and in vitro.
Clin Dev Immunol. 2010; 2010:357541.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD172a
- Product Type
- Monoclonal Antibody
- Clone
- OX-41
- Isotype
- IgG2a
- Specificity
- CD172a
Antibody Quality and Performance Guarantee
| Mouse anti Rat CD172a antibody, clone OX-41 recognizes rat CD172a, also known as Signal regulatory protein (SIRP). CD172a is expressed selectively by myeloid cells and by neurons. MRC OX-41 binds to a different epitope on SIRP than ED9. Mouse anti Rat CD172a antibody, clone OX-41 does not completely block the interaction of CD172a - CD47 (Vernon-Wilson et al. 2000). |
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Preparation
- MCA274G: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- MCA274R: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Rat peritoneal macrophages.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with the NS0/U mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/100 Pack Size: 1 mg 1/10 Pack Size: 0.25 mg |
1/200 Pack Size: 1 mg 1/50 Pack Size: 0.25 mg |
| Immunohistology - Frozen | |
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| Immunohistology - Paraffin 1 | |
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| Western Blotting | |
- 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA1210 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG2a Negative Control | ||||||
References for CD172a antibody
-
Robinson, A.P. et al. (1986) Macrophage heterogeneity in the rat as delineated by two monoclonal antibodies MRC OX-41 and MRC OX-42, the latter recognizing complement receptor type 3.
Immunology. 57 (2): 239-47. -
Santos, L.L. et al. (1997) Anti-neutrophil monoclonal antibody therapy inhibits the development of adjuvant arthritis.
Clin Exp Immunol. 107: 248-53. -
Adams, S. et al. (1998) Signal-regulatory protein is selectively expressed by myeloid and neuronal cells.
J Immunol. 161 (4): 1853-9. -
Milling, S.W. et al. (2006) Collection of lymph-borne dendritic cells in the rat.
Nat Protoc. 1: 2263-70. -
Moghaddami, M. et al. (2007) Recruitment of dendritic cells and macrophages during T cell-mediated synovial inflammation.
Arthritis Res Ther. 9: R120. -
Varalakshmi, Ch, et al. (2008) Immunomodulatory effects of curcumin: in-vivo.
Int Immunopharmacol. 8 (5): 688-700. -
Zhou, Y.J. et al. (2010) The role of the lactadherin in promoting intestinal DCs development in vivo and invitro.
Clin Dev Immunol. 2010: 357541.
- Synonyms
- SIRP ALPHA
- RRID
- AB_323480
- UniProt
- P97710
- Entrez Gene
- Sirpa
- GO Terms
- GO:0016477 cell migration
- GO:0017124 SH3 domain binding
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