CD172a antibody | OX-41

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Mouse anti Rat CD172a:Biotin

Mouse anti Rat CD172a:FITC

Mouse anti Rat CD172a

Product Type
Monoclonal Antibody
Clone
OX-41
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA274B C F P* 0.1 mg
MCA274F F 0.1 mg
MCA274GA C F P* 0.1 mg
MCA274R C F P* WB 0.25 mg
MCA274G C F P* WB 1 mg
Mouse anti Rat CD172a antibody, clone OX-41 recognizes rat CD172a, also known as Signal regulatory protein (SIRP). CD172a is expressed selectively by myeloid cells and by neurons.

MRC OX-41 binds to a different epitope on SIRP than ED9. It does not block the interaction of CD172a - CD47.

Product Details

Target Species
Rat
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1.0%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1.0%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Rat peritoneal macrophages.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
Isotype concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with the NS0/U mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P97710 Related reagents
Entrez Gene
Sirpa Related reagents
GO Terms
GO:0016477 cell migration
GO:0017124 SH3 domain binding
Regulatory
For research purposes only

Applications of CD172a antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Immunohistology - Frozen
Immunohistology - Paraffin 1
Flow Cytometry
Flow Cytometry 1/100 Pack Size: 0.1 mg, 1 mg
1/10 Pack Size: 0.25 mg
1/200 Pack Size: 0.1 mg, 1 mg
1/50 Pack Size: 0.25 mg
Immunohistology - Frozen
Immunohistology - Paraffin 1
Western Blotting
  1. 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
  1. 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG2a Negative Control:FITC MCA1210F 0.1 mg F
Mouse IgG2a Negative Control MCA1210 100 Tests F

Product Specific References

References for CD172a antibody

  1. Robinson, A.P. et al. (1986) Macrophage heterogeneity in the rat as delineated by two monoclonal antibodies MRC OX-41 and MRC OX-42, the latter recognizing complement receptor type 3.
    Immunology. 57 (2): 239-47.
  2. Barclay, A.N. (1981) The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues.
    Immunology. 42 (4): 593-600.
  3. Adams, S. et al. (1998) Signal-regulatory protein is selectively expressed by myeloid and neuronal cells.
    J Immunol. 161 (4): 1853-9.
  4. Santos, L.L. et al. (1997) Anti-neutrophil monoclonal antibody therapy inhibits the development of adjuvant arthritis.
    Clin Exp Immunol. 107: 248-53.
  5. Milling, S.W. et al. (2006) Collection of lymph-borne dendritic cells in the rat.
    Nat Protoc. 1: 2263-70.
  6. Moghaddami, M. et al. (2007) Recruitment of dendritic cells and macrophages during T cell-mediated synovial inflammation.
    Arthritis Res Ther. 9: R120.
  7. Zhou, Y.J. et al. (2010) The role of the lactadherin in promoting intestinal DCs development in vivo and invitro.
    Clin Dev Immunol. 2010: 357541.
  8. Varalakshmi, Ch, et al. (2008) Immunomodulatory effects of curcumin: in-vivo.
    Int Immunopharmacol. 8 (5): 688-700.