CD163 antibody | ED2

Product Code Applications Pack Size List Price Your Price Qty
MCA342GA
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SDS Safety Datasheet SDS
C F IF IP P * WB 0.1 mg loader
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loader
MCA342R
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SDS Safety Datasheet SDS
C F IF IP P * WB 0.25 mg loader
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loader

Mouse anti Rat CD163, clone ED2 recognizes the rat ED2 cell surface glycoprotein (Dijkstra et al. 1985). A 175 kDa molecule also known as rat CD163, a member of the group B scavenger receptor cysteine-rich (SRCR) family and an erythroblast adhesion receptor (Fabriek et al. 2007).

Mouse anti rat CD163, clone ED2 was shown to detect approximately 50% of peritoneal macrophages, a subset of splenic macrophages, and most tissue macrophages. However, no staining was observed in monocytes or alveolar macrophages (Dijkstra et al. 1985, Beelen et al. 1987). In freshly isolated bone marrow, expression of CD163 was limited to mature macrophages only (Barbe et al. 1990).

Clone ED2 may be used in immunohistology using antigen retrieval, and has also been described reacting with paraffin-embedded material following PLP fixation (Periodate-lysine-paraformaldehyde), see Whiteland et al.
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Our CD163 (ED2) Antibody has been referenced in >116 publications*


*Based on June 2020 data from CiteAb's antibody search engine.

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Rat Spleen cell homogenate.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0-Ag 14 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen 1/50 1/100
Immunohistology - Paraffin 1
Immunoprecipitation
Western Blotting
  1. 1 This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Histology Positive Control Tissue
Liver

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Description Mouse IgG1 Negative Control

References for CD163 antibody

  1. Dijkstra, C.D. et al. (1985) The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED3.
    Immunology. 54 (3): 589-99.
  2. Beelen, R.H.J. et al. (1987) Monoclonal antibodies ED1, ED2 and ED3 against rat macrophages: Expression of recognized antigens in different stages of differentiation.
    Transplant Proc. 3: 3166-70.
  3. Barbe, E. et al. (1990) Characterization and expression of the antigen present on resident rat macrophages recognized by monoclonal antibody ED2.
    Immunobiol. 182: 88-99.
  4. Dijkstra, C.D. & Damoiseaux, J.G. (1993) Macrophage heterogeneity established by immunocytochemistry.
    Prog Histochem Cytochem. 27 (2): 1-65.
  5. Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
    J Histochem Cytochem. 43 (3): 313-20.
  6. Muller, D.N. et al. (2002) Immunosuppressive treatment protects against angiotensin II-induced renal damage.
    Am J Pathol. 161: 1679-93.
  7. Polfliet, M.M.J. et al. (2002) Identification of the rat mature macrophage antigen ED2 as CD163: Regulation by glucocorticoids and role in the production of proinflammatory mediators.
    PhD Thesis. Vrije University, Amsterdam.
  8. Banerjee, S. et al. (2003) Development of organised conjunctival leucocyte aggregates after corneal transplantation in rats.
    Br J Ophthalmol. 87: 1515-22.
  9. View The Latest Product References
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  12. Moghaddami, M. et al. (2005) MHC class II compartment, endocytosis and phagocytic activity of macrophages and putative dendritic cells isolated from normal tissues rich in synovium.
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  14. Fabriek, B.O. et al. (2007) The macrophage CD163 surface glycoprotein is an erythroblast adhesion receptor.
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  15. Wehner, S. et al. (2007) Inhibition of macrophage function prevents intestinal inflammation and postoperative ileus in rodents.
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    Am J Pathol. 171: 872-81.
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    J Orthop Res. 26: 816-23.
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    Proc Natl Acad Sci U S A. 105: 11008-13.
  21. Yamato, M. et al. (2009) PET and macro- and microautoradiographic studies combined with immunohistochemistry for monitoring rat intestinal ulceration and healing processes.
    J Nucl Med. 50: 266-73.
  22. Santos, M. et al. (2009) An unbiased stereological study on subpopulations of rat liver macrophages and on their numerical relation with the hepatocytes and stellate cells.
    J Anat. 214: 744-51.
  23. Yu, Y. et al. (2010) Brain perivascular macrophages and the sympathetic response to inflammation in rats after myocardial infarction.
    Hypertension. 55: 652-9.
  24. Loureiro, J. et al. (2010) BMP-7 blocks mesenchymal conversion of mesothelial cells and prevents peritoneal damage induced by dialysis fluid exposure.
    Nephrol Dial Transplant. 25: 1098-108.
  25. Bedi, A. et al. (2010) Effect of early and delayed mechanical loading on tendon-to-bone healing after anterior cruciate ligament reconstruction.
    J Bone Joint Surg Am. 92: 2387-401.
  26. Harty, M.W. et al. (2010) Neutrophil depletion blocks early collagen degradation in repairing cholestatic rat livers.
    Am J Pathol. 176: 1271-81.
  27. Fujita, E. et al. (2010) Statin attenuates experimental anti-glomerular basement membrane glomerulonephritis together with the augmentation of alternatively activated macrophages.
    Am J Pathol. 177 (3): 1143-54.
  28. Zeng, Z. et al. (2010) Heme oxygenase-1 protects donor livers from ischemia/reperfusion injury: the role of Kupffer cells.
    World J Gastroenterol. 16: 1285-92.
  29. Dessem, D. et al. (2010) Eccentric muscle contraction and stretching evoke mechanical hyperalgesia and modulate CGRP and P2X(3) expression in a functionally relevant manner.
    Pain. 149: 284-95.
  30. Schwartzkopff, J. et al. (2010) NK cell depletion delays corneal allograft rejection in baby rats.
    Mol Vis. 16: 1928-35.
  31. Baker, S.C. et al. (2011) Cellular integration and vascularisation promoted by a resorbable, particulate-leached, cross-linked poly(ε-caprolactone) scaffold.
    Macromol Biosci. 11 (5): 618-27.
  32. Kawakami, A.P. et al. (2011) Inflammatory Process Modulation by Homeopathic Arnica montana 6CH: The Role of Individual Variation.
    Evid Based Complement Alternat Med. 2011: 917541.
  33. Kajita, M. et al. (2011) iNOS expression in vascular resident macrophages contributes to circulatory dysfunction of splanchnic vascular smooth muscle contractions in portal hypertensive rats.
    Am J Physiol Heart Circ Physiol. 300: H1021-31.
  34. Teng, B.T. et al. (2011) Protective effect of caspase inhibition on compression-induced muscle damage.
    J Physiol. 589: 3349-69.
  35. Wojcik, M. et al. (2012) Immunodetection of cyclooxygenase-2 (COX-2) is restricted to tissue macrophages in normal rat liver and to recruited mononuclear phagocytes in liver injury and cholangiocarcinoma.
    Histochem Cell Biol. 137: 217-33.
  36. Duchesne, E. et al. (2013) Mast cells can regulate skeletal muscle cell proliferation by multiple mechanisms.
    Muscle Nerve. 48 (3): 403-14.
  37. Fujii, Y. et al. (2013) Effect of enzymatically modified isoquercitrin on preneoplastic liver cell lesions induced by thioacetamide promotion in a two-stage hepatocarcinogenesis model using rats.
    Toxicology. 305: 30-40.
  38. Jiao, K. et al. (2013) The Identification of CD163 Expressing Phagocytic Chondrocytes in Joint Cartilage and Its Novel Scavenger Role in Cartilage Degradation.
    PLoS One. 8(1):e53312.
  39. Rave-Fränk M et al. (2013) Rat model of fractionated (2 Gy/day) 60 Gy irradiation of the liver: long-term effects.
    Radiat Environ Biophys. 52 (3): 321-38.
  40. Lobato-Pascual, A. et al. (2013) Rat macrophage C-type lectin is an activating receptor expressed by phagocytic cells.
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    Vet Pathol. 51 (6): 1151-64.
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    Biomaterials. 72: 125-37.
  43. Stavenuiter, A.W. et al. (2015) Protective Effects of Paricalcitol on Peritoneal Remodeling during Peritoneal Dialysis.
    Biomed Res Int. 2015: 468574.
  44. Zakrzewicz, A. et al. (2015) Monocytic Tissue Transglutaminase in a Rat Model for Reversible Acute Rejection and Chronic Renal Allograft Injury.
    Mediators Inflamm. 2015: 429653.
  45. Fernandez-Bustamante, A. et al. (2015) Brief Glutamine Pretreatment Increases Alveolar Macrophage CD163/Heme Oxygenase-1/p38-MAPK Dephosphorylation Pathway and Decreases Capillary Damage but Not Neutrophil Recruitment in IL-1/LPS-Insufflated Rats.
    PLoS One. 10 (7): e0130764.
  46. Hozumi, Y. et al. (2015) Expression and localization of the diacylglycerol kinase family and of phosphoinositide signaling molecules in adrenal gland.
    Cell Tissue Res. 362 (2): 295-305.
  47. Adamo, H.H. et al. (2015) Adaptive (TINT) Changes in the Tumor Bearing Organ Are Related to Prostate Tumor Size and Aggressiveness.
    PLoS One. 10 (11): e0141601.
  48. Tentillier N et al. (2016) Anti-Inflammatory Modulation of Microglia via CD163-Targeted Glucocorticoids Protects Dopaminergic Neurons in the 6-OHDA Parkinson's Disease Model.
    J Neurosci. 36 (36): 9375-90.
  49. Pannell, M. et al. (2016) Adoptive transfer of M2 macrophages reduces neuropathic pain via opioid peptides.
    J Neuroinflammation. 13 (1): 262.
  50. Almahrog, A.J. et al. (2016) In vivo. association of immunophenotyped macrophages expressing CD163 with PDGF-β in gingival overgrowth-induced by three different categories of medications.
    J Oral Biol Craniofac Res. 6 (1): 10-7.
  51. Ibarra, V. et al. (2016) Evaluation of the tissue response to alginate encapsulated islets in an omentum pouch model.
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    Exp Clin Transplant. 17 (1): 84-92.
  56. Bloomer, S.A. et al. (2020) Aging results in accumulation of M1 and M2 hepatic macrophages and a differential response to gadolinium chloride.
    Histochem Cell Biol. 153 (1): 37-48.
  57. Azam, M. et al. (2019) Addition of 2-deoxy-d-ribose to clinically used alginate dressings stimulates angiogenesis and accelerates wound healing in diabetic rats.
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  58. Li, R. et al. (2020) Bone marrow mesenchymal stem cell-derived exosomal microRNA-124-3p attenuates neurological damage in spinal cord ischemia-reperfusion injury by downregulating Ern1 and promoting M2 macrophage polarization.
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  72. Itoh, M. et al. (2022) Time-series biological responses toward decellularized bovine tendon graft and autograft for 52 consecutive weeks after rat anterior cruciate ligament reconstruction.
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  74. Hoff, U. et al. (2022) The mTOR inhibitor Rapamycin protects from premature cellular senescence early after experimental kidney transplantation.
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  75. Wu, C.C. et al. (2022) Preventive Intrathecal Injection of Bupivacaine Alleviated Microglia Activation and Neuropathic Pain in a Rat Model of Chronic Constriction Injury.
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  76. Köhler, R. et al. (2022) Association of systemic antibody response against polyethylene terephthalate with inflammatory serum cytokine profile following implantation of differently coated vascular prostheses in a rat animal model.
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    Cancer Immunol Immunother. 71 (4): 777-89.
  78. Pervin, M. et al. (2022) Possible Cytoprotection of Low Dose Lipopolysaccharide in Rat Thioacetamide-Induced Liver Lesions, Focusing on the Analyses of Hepatic Macrophages and Autophagy.
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  79. Yu, M. et al. (2022) Inhibition of Bruton's Tyrosine Kinase Alleviates Monocrotaline-Induced Pulmonary Arterial Hypertension by Modulating Macrophage Polarization.
    Oxid Med Cell Longev. 2022: 6526036.

Flow Cytometry

Functional Assays

Immunocytochemistry

Immunofluorescence

Immunohistology - Frozen

Immunohistology - Paraffin

Western Blotting

Synonyms
ED2
RRID
AB_321966

MCA342GA

147541 156103 162985

MCA342R

158552 1705

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