CD8 Alpha Antibody | OX-8

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CD8 Alpha Antibody | OX-8 gallery image 1

Staining of rat peripheral blood cells with Mouse anti Rat CD8 Alpha Chain:Alexa Fluor® 488 (MCA48A488)

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CD8 Alpha Antibody | OX-8 gallery image 2

Published customer image:
Mouse anti Rat CD8 alpha antibody, clone OX-8 used for the identification of cytotoxic T cells in tumors by immunofluorescence.
Image caption:
Immunohistochemical labeling of tumor thin sections with CD8 (red) antibodies and DAPI (blue) nuclear stain. A: Two typical sections from an untreated control tumor collected 14 days after injecting tumor cells into the liver; B. Two typical sections of a secondary tumor that was fixed 7 days after it was injected into a liver in which the primary tumor had been nanoelectroablated 3 weeks earlier. This secondary tumor exhibited growth inhibition and a photograph of it can be seen in Fig 5B; C. Percentage of cells expressing CD8 in histological sections of tumors from four different conditions. Each bar represents the mean of measurements taken from 3–7 sections of 2 separate tumors. The untreated tumors were fixed 14 days after injection; “ns+7d” represents tumors fixed 7days after treating with 400 pulses, 15 kV, 100ns; “T2+7d” represents secondary tumors fixed 7 days after injection. CD8 concentration is significantly different from control tumor levels with **p = 0.002; “T2+7d+CD9 AB” represents secondary tumors fixed 7 days after injection with CD8 antibodies injected IP 24 h prior to the tumor cell injection. The CD8 concentration is significantly different from control tumors with *p = 0.005.

From:Citation: Nuccitelli R, Berridge JC, Mallon Z, Kreis M, Athos B, Nuccitelli P (2015) Nanoelectroablation of Murine Tumors Triggers a CD8-Dependent Inhibition of Secondary Tumor Growth.
PLoS ONE 10(7): e0134364.

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CD8 Alpha Antibody | OX-8 gallery image 3

Published customer image:
Mouse anti Rat CD8α antibody, clone OX-8 used for the detection of CD8 positive cells by flow cytometry.
Image caption
Qualitative and quantitative flow cytometric analysis of lymphocyte populations in draining lymph nodes. A: Representative FACS plot of CD4+ CD8+ staining used to count T-helper cells, cytotoxic T-cells and CD4+ CD8+ double positive T-lymphocytes. Events acquired: 2×105. B: FACS plot example for B-cell detection. C: Representative FACS plot for NK cell assessment. NK-T cell were confirmed by CD3 expression (not shown). Bar diagrams: Cumulative results for the quantification of major and minor lymphocyte populations in draining LN of cornea transplanted animals. An asterisk (*) indicates statistical significance at p=0.05 determined by Mann–Whitney U-Test. Allo-Tx-d7 - animals allo-grafted and analyzed at day 07 post op, n=6; allo-Tx-rej – animals displaying allo rejection of grafted corneas analyzed after the onset of rejection, n=5; syn-Tx-d7 – syngeneically grafted animals analyzed at day 7 post-op, n=3; syn-Tx-LT – syn-grafted long-term survivors analyzed at the end of the observation period at day 42; n=3.

Maenz M, Morcos M, Ritter T. A comprehensive flow-cytometric analysis of graft infiltrating lymphocytes, draining lymph nodes and serum during the rejection phase in a fully allogeneic rat cornea transplant model. Mol Vis. 2011 Feb 8;17:420-9.

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CD8 Alpha Antibody | OX-8 gallery image 4

Published customer image:
Mouse anti Rat CD8α antibody, clone OX-8 used for the detection of CD8 positive cells by immunohistochemistry.
Image caption
CD4 and CD8 T cell infiltration. Photos show SN sections of an animal of the cell death group immunostained with antibody against CD4 (A and C) and CD8 (B and D). The small panels show insets in A (C) and in B (D) at higher magnification. Scale: 50 µm, applies to A–B, 10 µm applies to C–D. (E) Graph shows average (dash) and individual numbers of CD4+ cells found in one SN section per animal of each group plotted per time. Two-way ANOVA [F (8,42) = 4.1, p = 0.001 effect of group and time interaction] followed by Tukey HSD post-hoc analysis. ## or ç p<0.01 (##) compared to the other a-syn group at same time point; (††) different to the next time point of the same group. (F) Graph shows average (dash) and individual numbers of CD8+ cells found in one SN section per animal of each group plotted per time. Two-way ANOVA [F (8,41) = 4.3, p = 0.001 effect of group and time interaction] followed by Tukey HSD post-hoc analysis. (**) p<0.01 compared to all other groups at all time point.

From: Sanchez-Guajardo V, Febbraro F, Kirik D, Romero-Ramos M (2010) Microglia Acquire Distinct Activation Profiles Depending on the Degree of α-Synuclein Neuropathology in a rAAV Based Model of Parkinson's Disease.
PLoS ONE 5(1): e8784.

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CD8 Alpha Antibody | OX-8 gallery image 5

Published customer image:
Mouse anti Rat CD8α antibody, clone OX-8 used for the detection of CD8 positive cells by immunohistochemistry.
Image caption
Analysis of graft-infiltrating T cells. Activated CD25+ T cells and CD4+ and CD8+ T cell subsets were stained at the time points of corneal allograft rejection and calculated within the graft. A, C, and E show representative histological staining for CD25, CD4, and CD8 in grafts of treated and control animals, respectively. CD25+ (B) and CD4+ (D) cells infiltrated to a statistically significantly stronger extent in 3.2.3-treated animals when compared to control treated animals (*p<0.01, ** p<0.01). No statistical difference was observed for CD8+ T cells (NS=not significant; F).

From: Schwartzkopff J, Schlereth SL, Berger M, Bredow L, Birnbaum F, Böhringer D, Reinhard T. NK cell depletion delays corneal allograft rejection in baby rats. Mol Vis. 2010 Oct 2;16:1928-35.

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CD8 Alpha Antibody | OX-8 gallery image 6

Published customer image:
Mouse anti Rat CD8α antibody, clone OX-8 used for the detection of CD8 expressing cells in Bouin's fixed, paraffin embedded tissue sections by immunofluorescence.
Image caption:
CD3ε, CD8α, and PAX5 immunostainings. (A–D) CD3ε staining in the zone of the foramen of Monro (fM) of the injected ventricle comprising a part of the stria medullaris [sm, (A)]. Many positive cells occurred in the perivascular spaces of the local large venules 12 h after NA injection. A detailed view (B) of the vessels in the stria medullaris (sm) shows that many positive cells have smooth nuclei while polymorphonucleated cells (arrow) are negative. (C) Twenty-four hours after the injection, some positive cells seemed to cross the ependyma of the choroid plexus (cp). (D) Positive cells were also present in the meninges (men) next to the optic chiasm (oc), and few appeared to penetrate the brain parenchyma (arrow). (E–G) Double immunofluorescence of lymphocytes in a 12 h post-injection brain section. Most CD3ε-positive cells [(E), green] found in the perivascular spaces of the large vessels were also positive for CD8α [(F), red]. (H–J) PAX5 immunostaining showing positive cells in the wall of the large vessels around the foramen of Monro [fM; (H)], although they were not as abundant as CD3ε-positive cells. Few PAX5-positive nuclei were also found in the choroid plexus (cp) of the injected lateral ventricle (I) as well as in the meninges (men) close to the optic chiasm [oc; (J)]. cp, choroid plexus; fM, foramen of Monro; men, meninges; oc, optic chiasm; sm, stria medullaris.

From: Granados-Durán P, López-Ávalos MD, Grondona JM, Gómez-Roldán Mdel C, Cifuentes M, Pérez-Martín M, Alvarez M, Rodríguez de Fonseca F, Fernández-Llebrez P. Neuroinflammation induced by intracerebroventricular injection of microbial neuraminidase. Front Med (Lausanne). 2015 Mar 17;2:14.

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CD8 Alpha Antibody | OX-8 gallery image 7

Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD25 antibody, clone OX-35 (MCA153), red in A and Mouse anti Rat CD8, clone OX-8 (MCA48), green in B. C is the merged image with nuclei counterstained blue using DAPI. High power

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CD8 Alpha Antibody | OX-8 gallery image 8

Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD25 antibody, clone OX-35 (MCA153), red in A and Mouse anti Rat CD8 antibody, clone OX-8 (MCA48), green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power

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CD8 Alpha Antibody | OX-8 gallery image 9

Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD25 antibody, clone OX-35 (MCA153), red in A and Mouse anti Rat CD8 antibody, clone OX-8 (MCA48), green in B. C is the merged image with nuclei counterstained blue using DAPI. High power

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CD8 Alpha Antibody | OX-8 gallery image 10

Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD25 antibody, clone OX-35 (MCA153), red in A and Mouse anti Rat CD8 antibody, clone OX-8 (MCA48), green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power

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CD8 Alpha Antibody | OX-8 gallery image 11

Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD11b antibody, clone OX-42 (MCA275), red in A and Mouse anti Rat CD8 antibody, clone OX-8 (MCA48), green in B. C is the merged image with nuclei counter-stained in blue using DAPI. High power

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CD8 Alpha Antibody | OX-8 gallery image 12

Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody, clone ED2 (MCA342), red an A and Mouse anti Rat CD8 antibody, clone OX-8 (MCA48), green in B. C is the merged image with nuclei counter-stained blue using DAPI. High power

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  • Mouse anti Rat CD8 Alpha:RPE-Alexa Fluor® 647
  • Mouse anti Rat CD8 Alpha
  • Mouse anti Rat CD8 Alpha:FITC
  • Mouse anti Rat CD8 Alpha
  • Mouse anti Rat CD8 Alpha:RPE
  • Mouse anti Rat CD8 Alpha:FITC
  • Mouse anti Rat CD8 Alpha:Alexa Fluor® 488
  • Mouse anti Rat CD8 Alpha:Alexa Fluor® 647
  • Mouse anti Rat CD8 Alpha:FITC
  • Mouse anti Rat CD8 Alpha:Low Endotoxin
  • Mouse anti Rat CD8 Alpha
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    OX-8
  • Isotype
    IgG1
7 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA48GAC, F, IP, Pdatasheet pdfdatasheet pdf0.1 mg
    MCA48GA
    MCA48FTFdatasheet pdfdatasheet pdf0.1 mg
    MCA48FT
    MCA48RC, F, IF, IP, P, WBdatasheet pdfdatasheet pdf0.25 mg
    MCA48R
    MCA48FBFdatasheet pdfdatasheet pdf0.5 mg
    MCA48FB
    MCA48ELC, F, FN, IP, P *, WBdatasheet pdfdatasheet pdf0.5 mg
    MCA48EL
    MCA48GC, F, IF, IP, P, WBdatasheet pdfdatasheet pdf1 mg
    MCA48G
    MCA48PEFdatasheet pdfdatasheet pdf100 Tests
    MCA48PE
    MCA48P647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA48P647
    MCA48A488Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA48A488
    MCA48A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA48A647
    MCA48FAFdatasheet pdfdatasheet pdf50 µg
    MCA48FA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Rat CD8α, clone MRC OX-8, recognizes the rat CD8 alpha cell surface antigen, expressed by a subset of T lymphocytes, most thymocytes and the majority of NK cells.

      Clone MRC OX-8 is suitable for use in in vitro blocking studies (Popov et al.2001).

      Mouse anti Rat CD8α, clone MRC OX-8 has been described reacting with paraffin-embedded material following PLP Fixation (periodate-lysine paraformaldehyde) (Whiteland et al. 1995).

      Mouse anti Rat CD8α, clone MRC OX-8 is routinely tested in flow cytometry on rat splenocytes.
    • Intended Use
    • Target Species
      Rat
    • Product Form
      Purified IgG conjugated to R. Phycoerythrin (RPE) - Alexa Fluor® 647 - lyophilized
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Alexa Fluor® 488 - liquid
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
      Reconstitute with 1.0 ml distilled water
      Reconstitute with 1 ml distilled water
    • Preparation
      Purified IgG prepared by ion exchange chromatography from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
      Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G
      Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
      Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      None present
      0.09%Sodium Azide
    • Immunogen
      Rat thymocyte membrane glycoproteins.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      Pack Size: 50 µg, 0.1 mgIgG concentration 0.1 mg/ml
      Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
      IgG concentration 1.0 mg/ml
      Pack Size: 50 µg, 0.1 mgIgG concentration 0.1 mg/ml
      Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 0.05 mg/ml
      Pack Size: 50 µg, 0.1 mgIgG concentration 0.1 mg/ml
      Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
    • Storage
      Store at +4oC.
      DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 0.25 mgStore at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 1 mg, 0.1 mgStore at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 0.25 mgStore at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 1 mg, 0.1 mgStore at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at -20oC only.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 0.25 mgStore at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 1 mg, 0.1 mgStore at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      integral to membrane
      protein kinase binding
      inflammatory response
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Immunofluorescence
      Immunohistology - Frozen
      Immunohistology - Paraffin
      Immunoprecipitation
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      1/5Pack Size: 0.5 mg
      NeatPack Size: 0.1 mg
      1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Immunofluorescence
      Immunohistology - Frozen
      Immunohistology - Paraffin
      Immunoprecipitation
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      1/5Pack Size: 0.5 mg
      NeatPack Size: 0.1 mg
      1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      1/5Pack Size: 0.5 mg
      NeatPack Size: 0.1 mg
      1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/251/50
      Functional Assays
      Immunohistology - Frozen
      Immunoprecipitation
      Western Blotting1/1001/500
      Immunohistology - Paraffin(1)
      (1)
      This product does not require protein digestion pre-treatment of paraffin embedded sections.
      This product does not require antigen retrieval using heat treatment prior to staining of paraffin embedded sections.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Immunofluorescence
      Immunohistology - Frozen
      Immunohistology - Paraffin
      Immunoprecipitation
      Western Blotting

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
      This product does not require protein digestion pre-treatment of paraffin embedded sections.
      This product does not require antigen retrieval using heat treatment prior to staining of paraffin embedded sections.
    • Immunohistology
    • Immunohistology
      This product does not require protein digestion pre-treatment of paraffin embedded sections.
      This product does not require antigen retrieval using heat treatment prior to staining of paraffin embedded sections.
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
      This product does not require protein digestion pre-treatment of paraffin embedded sections.
      This product does not require antigen retrieval using heat treatment prior to staining of paraffin embedded sections.
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD8 Alpha Antibody Formats

    Formats Clone Applications Sizes available
    CD8 Alpha Antibody : FITC OX-8 F 0.5 mg | 50 µg | 0.1 mg
    CD8 Alpha Antibody : Purified OX-8 C, F, IF, IP, P, WB 1 mg | 0.1 mg | 0.25 mg
    CD8 Alpha Antibody : Low Endotoxin OX-8 C, F, FN, IP, P *, WB 0.5 mg
    CD8 Alpha Antibody : Alexa Fluor® 647 OX-8 F 100 Tests/1ml
    CD8 Alpha Antibody : RPE-Alexa Fluor® 647 OX-8 F 100 Tests/1ml
    CD8 Alpha Antibody : RPE OX-8 F 100 Tests
    CD8 Alpha Antibody : Alexa Fluor® 488 OX-8 F 100 Tests/1ml
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF, WB
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      HCA036P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF, WB
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      HCA036P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF, WB
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      HCA036P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF, WB
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      HCA036P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

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        MCA1209F
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        MCA1209
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        Mouse IgG1 Negative Control:RPEMCA1209PE100 TestsF
        MCA1209PE
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        Mouse IgG1 Negative Control:Alexa Fluor® 488MCA1209A488100 Tests/1mlF
        MCA1209A488
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:Alexa Fluor® 647MCA1209A647100 Tests/1mlF
        MCA1209A647
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:FITCMCA1209F0.1 mgF
        MCA1209F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:Low EndotoxinMCA1209EL0.5 mgF
        MCA1209EL
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA12090.1 mgF
        MCA1209

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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