IgG1 Antibody

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IgG1 Antibody gallery image 1

Immunoperoxidase staining of rat lymph node cryosetion using Mouse anti Rat CD4 antibody (MCA55) followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (STAR132P) for detection. Low power

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IgG1 Antibody gallery image 2

Immunoperoxidase staining of rat lymph node cryosetion using Mouse anti Rat CD4 antibody (MCA55) followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (STAR132P) for detection. Medium power

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IgG1 Antibody gallery image 3

Immunoperoxidase staining of rat lymph node cryosetion using Mouse anti Rat CD4 antibody (MCA55) followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (STAR132P) for detection. High power

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IgG1 Antibody gallery image 4

Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody (MCA341) followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (STAR132P) as a detection reagent. High power

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IgG1 Antibody gallery image 5

Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody (MCA342) followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (STAR132P) as a detection reagent. Medium power

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IgG1 Antibody gallery image 6

Published customer image:
Phycoerythrin conjugated Goat anti Mouse IgG1 antibody used for the detection of bound antibody on lymphoblastoid cell lines by flow cytometry.
Image caption:
Estimation of ΔBNLF2a and wild-type LCLs expressing lytic antigens; recognition by immediate early antigen-specific CD8+ T cells. The proportion of LCLs spontaneously reactivating into lytic cycle was assessed by intracellular BZLF1 staining and analysis by flow cytometry, with representative examples shown for LCLs derived from two different donors: (A) donor 1 and (B) donor 2. Immediate early lytic cycle CD8+ T cell recognition of wild-type (wt), ΔBNLF2a (Δ2a) and ΔBZLF1 (ΔBZ) LCLs using HLA-B*0801-restricted RAK (BZLF1) clones against appropriately HLA matched donor 1 and 2 LCLs (C and D respectively) was measured by IFNγ ELISA. Results using wild-type or ΔBNLF2a cells diluted with ΔBZLF1 cells as appropriate are shown, where arrows indicate equivalent numbers of lytic antigen expressing cells. Experiments were also conducted using HLA-C*0202-restricted IACP (BRLF1) clones against donor 3 LCLs (E), and HLA-B*4501-restricted AEN (BRLF1) clones against donor 4 LCLs (F). For donor 4, both the wild-type and ΔBNLF2a LCLs were diluted with ΔBZLF1 LCL (wild-type-LCL titration data not shown). Data are represented as mean+/-SEM.

From: Croft NP, Shannon-Lowe C, Bell AI, Horst D, Kremmer E, et al. (2009) Stage-Specific Inhibition of MHC Class I Presentation by the Epstein-Barr Virus BNLF2a Protein during Virus Lytic Cycle. PLoS Pathog 5(6): e1000490.

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IgG1 Antibody gallery image 7

Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody (MCA342) followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (STAR132P) as a detection reagent. High power

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IgG1 Antibody gallery image 8

Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD11 antibody, clone ED8 (MCA619) followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (STAR132P) as a detection reagent. Low power

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IgG1 Antibody gallery image 9

Published customer image:
Horseradish peroxidase conjugated Goat anti Mouse IgG1 antibody used for the evaluation of rotavirus specific IgG1 antibodies in mouse serum be ELISA.
Image caption:
Rotavirus-specific IgG-subclass antibodies in serum. Geometric mean titers (GMT) in arbitrary units (AU) ± SEM of the rotavirus-specific IgG-subclass antibodies IgG1, IgG2a, IgG2b and IgG3 in serum individually collected at day 28, depicted per antibody.

From: Knipping et al.
A gastrointestinal rotavirus infection mouse model for immune modulation studies
Virology Journal 2011 8:109.

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IgG1 Antibody gallery image 10

Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD11 antibody, clone ED8 (MCA619) followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (STAR132P) as a detection reagent. High power

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IgG1 Antibody gallery image 11

Published customer image:
FITC conjugated Goat anti Mouse IgG1 antibody used for the detection of bound Mouse anti BrdU antibody by immunofluorescence.
Image caption:
Sequential labelling using EdU and BrdU. Microscopy pictures of cells double-labelled with EdU and BrdU. Cells synchronized in G1 phase were released and pulse-labelled in two consecutive S-phases, first with EdU, then with BrdU, as described in the text. Samples were harvested after the next mitosis, fixed and processed as described and imaged using fluorescence microscopy.

From: Anda S, Boye E, Grallert B (2014) Cell-Cycle Analyses Using Thymidine Analogues in Fission Yeast. PLoS ONE 9(2): e88629.

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IgG1 Antibody gallery image 12

Published customer image:
FITC conjugated Goat anti Mouse IgG1 antibody used for the detection of bound Mouse anti BrdU antibody by immunofluorescence.
Image caption:
Microscopy pictures of cells labelled with EdU or BrdU. Cells were labelled with either analogue and detection for both analogues was performed to check cross-reactivity.

From: Anda S, Boye E, Grallert B (2014) Cell-Cycle Analyses Using Thymidine Analogues in Fission Yeast. PLoS ONE 9(2): e88629.

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IgG1 Antibody gallery image 13

Figure A. FITC conjugated mouse anti porcine CD4a (MCA1749F) and purified mouse IgG1 isotype control (MCA928) detected with goat anti mouse IgG1PE (STAR132PE). Figure B. FITC conjugated mouse anti porcine CD4a (MCA1749F) and purified mouse anti porcine CD25 (MCA1736GA) detected with goat anti mouse IgG1PE (STAR132PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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IgG1 Antibody gallery image 14

Figure A. Purified mouse anti porcine CD25 (MCA1736GA) detected with goat anti mouse IgG1 PE (STAR132PE) and mouse IgG2b FITC isotype control (MCA691F). Figure B. Purified mouse anti porcine CD25 (MCA1736GA) detected with goat anti mouse IgG1 PE (STAR132PE) and mouse anti porcine CD4a FITC (MCA1749F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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IgG1 Antibody gallery image 15

Figure A. FITC conjugated mouse anti porcine CD11a (MCA2308F) and purified mouse IgG1 isotype control (MCA928) detected with goat anti mouse IgG1 PE (STAR132PE). Figure B. FITC conjugated mouse anti porcine CD11a (MCA2308F) and purified mouse anti porcine CD11R1 (MCA1220) detected with goat anti mouse IgG1 (STAR132PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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IgG1 Antibody gallery image 16

Figure A. Purified mouse anti porcine CD11R1 (MCA1220) detected with goat anti mouse IgG PE (STAR132PE) and mouse IgG2b FITC isotype control (MCA691F). Figure B. Purified mouse anti porcine CD11R1 (MCA1220) detected with goat anti mouse IgG1 PE (STAR132PE) and mouse anti porcine CD11a FITC (MCA5951F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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IgG1 Antibody gallery image 17

Figure A. FITC conjugated mouse anti porcine CD11a (MCA2308F) and purified mouse IgG1 isotype control (MCA928) detected with goat anti mouse IgG1 PE (STAR132PE). Figure B. FITC conjugated mouse anti porcine CD11a (MCA2308F) and purified mouse anti porcine CD46 (MCA2262) detected with goat anti mouse IgG1 (STAR132PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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IgG1 Antibody gallery image 18

Figure A. Purified mouse anti porcine CD46 (MCA2262) detected with goat anti mouse IgG1 PE (STAR132PE) and mouse IgG2b FITC isotype control (MCA691F). Figure B. Purified mouse anti porcine CD46 (MCA2262) detected with goat anti mouse IgG1 PE (STAR132PE) and mouse anti porcine CD11a FITC (MCA5951F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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  • Goat anti Mouse IgG1:RPE
  • Goat anti Mouse IgG1:FITC
  • Goat anti Mouse IgG1:HRP
  • Goat anti Mouse IgG1
  • Goat anti Mouse IgG1:Alk. Phos.
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
5 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    STAR132PEFdatasheet pdfdatasheet pdf0.1 mg
    STAR132PE
    STAR132FC, F, IFdatasheet pdfdatasheet pdf0.5 mg
    STAR132F
    STAR132Edatasheet pdfdatasheet pdf0.5 mg
    STAR132
    STAR132PC, E, P, WBdatasheet pdfdatasheet pdf1 ml
    STAR132P
    STAR132AC, E, P, WBdatasheet pdfdatasheet pdf1 ml
    STAR132A
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Goat anti Mouse IgG1 antibody recognizes Mouse IgG1. This antibody has been cross absorbed against mouse IgM, IgG2a, IgG2b, IgG3 and IgA, pooled human sera and purified human paraproteins. Goat anti Mouse IgG1 antibody shows minimal cross-reactivity with human immunoglobulins.
    • Intended Use
    • Target Species
      Mouse
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Humanno
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Alkaline Phosphatase - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to mouse IgG1 were raised by repeated immunisation of goats with purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.09%Sodium Azide
      Sucrose
      0.1% Sodium Azide (NaN3)
      0.01% Thiomersal
      50% Glycerol
      0.09%Sodium Azide
      0.1%Sodium Azide (NaN3)
      50% Glycerol
    • Immunogen
      Mouse IgG1 paraproteins.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5mg/ml
      IgG concentration 1.0mg/ml
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Borate buffered saline
      50mM TRIS
      1mM MgCl2
    • Storage
      Store at +4oC.

      DO NOT FREEZE

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      6 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      integral to membrane
      positive regulation of type IIa hypersensitivity
      positive regulation of type I hypersensitivity
      phagocytosis, engulfment
      complement activation, classical pathway
      antibacterial humoral response
      plasma membrane
      positive regulation of phagocytosis
      immunoglobulin complex, circulating
      antibody-dependent cellular cytotoxicity
      antigen binding
      phagocytosis, recognition
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/201/30
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
      ImmunofluorescenceNeat1/10
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/40001/8000
      Immunohistology - Frozen
      Immunohistology - Paraffin
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/20001/4000
      Immunohistology - Frozen
      Immunohistology - Paraffin
      Western Blotting

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional IgG1 Antibody Formats

    Formats Applications Sizes available
    IgG1 Antibody : RPE F 0.1 mg
    IgG1 Antibody : FITC C, F, IF 0.5 mg
    IgG1 Antibody : HRP C, E, P, WB 1 ml
    IgG1 Antibody : Alk. Phos. C, E, P, WB 1 ml
    IgG1 Antibody : Purified E 0.5 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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      STAR122P

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          AbGUARD® HRP Stabilizer PlusBUF052A100 mlC, E, P, WB
          BUF052A
          TMB Signal+BUF054A100 mlE
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          BUF052C
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          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry

              References

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