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CD4 Alpha antibody | MIL17

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Anti Pig CD4 Alpha Antibody, clone MIL17 thumbnail image 1 Anti Pig CD4 Alpha Antibody, clone MIL17 thumbnail image 2 Anti Pig CD4 Alpha Antibody, clone MIL17 thumbnail image 3 Anti Pig CD4 Alpha Antibody, clone MIL17 thumbnail image 4 Anti Pig CD4 Alpha Antibody, clone MIL17 thumbnail image 5 Anti Pig CD4 Alpha Antibody, clone MIL17 thumbnail image 6 Anti Pig CD4 Alpha Antibody, clone MIL17 thumbnail image 7 Anti Pig CD4 Alpha Antibody, clone MIL17 thumbnail image 8
Anti Pig CD4 Alpha Antibody, clone MIL17 gallery image 1
Figure A. RPE conjugated mouse anti porcine CD45a antibody, clone MIL17 (MCA1751PE) and FITC conjugated mouse IgM isotype control (MCA691F). Figure B. RPE conjugated mouse anti porcine CD45a (MCA1751PE) and FITC conjugated mouse anti porcine CD4 (MCA1749F). All experiments performed on red cell lysed porcine blood gated on lymphocytes. Data acquired on the ZE5™ Cell Analyzer.
Anti Pig CD4 Alpha Antibody, clone MIL17 gallery image 2
Figure A. Alexa Fluor 647 conjugated mouse anti porcine CD45 (MCA1222A647) and FITC conjugated mouse IgM isotype control (MCA691F). Figure B. Alexa Fluor 647 conjugated mouse anti porcine CD45 (MCA1222A647) and FITC conjugated mouse anti porcine CD4 (MCA1749F). All experiments performed on red cell lysed porcine blood gated on lymphocytes. Data acquired on the ZE5™ Cell Analyzer.
Anti Pig CD4 Alpha Antibody, clone MIL17 gallery image 3
Published customer image:
Mouse anti Porcine CD4 alpha, clone MIL17 (MCA1749GA) used for the evaluation of CD4a expression on porcine peripheral blood mononuclear cells by flow cytometry.
Image caption:
T cell sub-population proliferation monitored by CFSE labeling in response to in vitro re-stimulation with H1N1 influenza. CFSE-labeled PBMC were cultured in vitro in the presence of 105EID50/ml inactivated Eng195, uninfected allantoic fluid (mock antigen), PWM or in media only. After 5 days, cells were stained with anti-CD4 and anti-CD8 monoclonal antibodies and analyzed by flow cytometry for CFSE intensity. Three T cell subsets were identified on the basis of their CD4 and CD8 expression, and an example of the CFSE pattern at day 7pv in response to inactivated Eng195 in these various subsets in a pig vaccinated with the adjuvanted split vaccine is shown (A). At day 28pv, the percentage of cells proliferating (CFSELow cells) in response to media alone, mock antigen or inactivated Eng195 was determined after gating on CD4+CD8− (B), CD4+CD8+ (C) or CD4−CD8+ (D) cells. Results are expressed as the mean percentage of proliferating (CFSELow) cells ± SEM. n.s = Not statistically significant.

From: Lefevre EA, Carr BV, Inman CF, Prentice H, Brown IH, Brookes SM, et al. (2012)
Immune Responses in Pigs Vaccinated with Adjuvanted and Non-Adjuvanted A(H1N1)pdm/09 Influenza Vaccines Used in Human Immunization Programmes.
PLoS ONE 7(3): e32400.
Anti Pig CD4 Alpha Antibody, clone MIL17 gallery image 4
Published customer image:
Mouse anti Porcine CD4 alpha, clone MIL17 (MCA1749GA) used for the identification of CD4 expressing cells in procine jejunal mucosa by immunofluorescence.
Image caption:
Colonisation expands mucosal CD4+ T cells by 21 days of age. (A) CD4 staining in jejunal mucosa from a GF pig and (B) a colonised pig at 21 days of age. Sections were stained with antibodies to CD4 (green) and capillary endothelium (MIL11; in blue). In both (A) and (B), (i) shows the two-colour image; (ii) shows the original greyscale image for the blue channel (endothelium); (iii) shows the original greyscale image for the green channel (CD4). Scale bar represents 10 μm. (C) Area of CD4+ T cell staining in jejunal sections from piglets at birth (open diamonds) and GF piglets (open squares) and colonized piglets (black squares) at 5 and 21 days of age (see Table 1 for numbers of piglets in each experiment). *p<0.05.

From: Inman CF, Laycock GM, Mitchard L, Harley R, Warwick J, Burt R, et al. (2012)
Neonatal Colonization Expands a Specific Intestinal Antigen-Presenting Cell Subset Prior to CD4 T-Cell Expansion, without Altering T-Cell Repertoire.
PLoS ONE 7(3): e33707.
Anti Pig CD4 Alpha Antibody, clone MIL17 gallery image 5
Figure A. FITC conjugated Mouse anti Porcine CD5 (MCA2307F) and RPE conjugated Mouse IgG2a isotype control (MCA929PE). Figure B. FITC conjugated Mouse anti Porcine CD5 (MCA2307F) and RPE conjugated Mouse anti Porcine CD4a (MCA1749PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells
Anti Pig CD4 Alpha Antibody, clone MIL17 gallery image 6
Figure A. RPE conjugated Mouse anti Porcine CD4a (MCA1749PE) and FITC conjugated mouse IgG2b isotype control (MCA691F). Figure B. RPE conjugated Mouse anti Porcine CD4a (MCA1749PE) and FITC conjugated Mouse anti Porcine CD5 (MCA2307F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Anti Pig CD4 Alpha Antibody, clone MIL17 gallery image 7
Figure A. FITC conjugated Mouse anti Porcine CD4a (MCA1749F) and Purified mouse IgG1 isotype control (MCA928) detected with Goat anti Mouse IgG1PE (STAR132PE). Figure B. FITC conjugated Mouse anti Porcine CD4a (MCA1749F) and purified Mouse anti Porcine CD25 (MCA1736GA) detected with Goat anti Mouse IgG1PE (STAR132PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Anti Pig CD4 Alpha Antibody, clone MIL17 gallery image 8
Figure A. Purified mouse anti porcine CD25 (MCA1736GA) detected with goat anti mouse IgG1 PE (STAR132PE) and mouse IgG2b FITC isotype control (MCA691F). Figure B. Purified mouse anti porcine CD25 (MCA1736GA) detected with goat anti mouse IgG1 PE (STAR132PE) and mouse anti porcine CD4a FITC (MCA1749F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

Mouse anti Pig CD4 Alpha:FITC

Mouse anti Pig CD4 Alpha

Mouse anti Pig CD4 Alpha:RPE

Product Type
Monoclonal Antibody
Clone
MIL17
Isotype
IgG2b
Product Code Applications Pack Size List Price Quantity
MCA1749F
Datasheet
F
MSDS
0.1 mg loader

MCA1749GA
Datasheet
C* F
MSDS
0.1 mg loader

MCA1749PE
Datasheet
F
MSDS
100 Tests loader

Mouse anti Porcine CD4 alpha, clone MIL17 recognizes a ~55 kDa porcine homologue to the human CD4 antigen found on the surface of helper-T cells. MIL-17 was confirmed as a member of the CD4 alpha cluster at the ‘Third International Workshop on Swine Leukocyte Differentiation Antigens’ (Haverson et al. 2001). Porcine CD4 is a type 1 trans-membrane member of the immunoglobulin superfamily.

Pigs appear unusual amongst mammalian species as they appear to have four populations of resting T lymphocytes. In addition to the two populations of mutually exclusive CD4+/CD8- and CD4-/CD8+ lymphocytes, they also appear to have significant populations of CD4-/CD8- and CD4+/CD8+ cells. Lymphoblasts with a double positive phenotype have been described in other species but this is not the case for mature T lymphocytic calls (Saalmuller et al. 1987)

Mouse anti Pig CD4 alpha, clone MIL17 stains a population of cells with characteristic lymphocyte morphology in immunohistochemistry (Inman et al. 2010).

Product Details

Target Species
Pig
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Carrier Free
Yes
Immunogen
Leucocytes isolated from porcine gut lamina propria.
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.
Guarantee
18 months from date of despatch
Guarantee
12 months from date of reconstitution.

More Information

Regulatory
For research purposes only

Applications of CD4 Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry 1/25 1/200
Immunohistology - Frozen 1
Immunohistology - Paraffin
Flow Cytometry Neat
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgG2b:FITC HCA038F F 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG2b Negative Control:FITC MCA691F F 100 Tests loader
Mouse IgG2b Negative Control MCA691 F 100 Tests loader
Mouse IgG2b Negative Control:RPE MCA691PE F 100 Tests loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Pig CD45 MCA1222GA C F IF 0.1 mg loader
Mouse anti Pig wCD8 Alpha MCA1223GA C F 0.1 mg loader
Rat anti Human CD3 MCA1477 C F* IF P* WB 0.2 mg loader

Application Based External Images

Flow Cytometry

Immunofluorescence

Immunohistology - Frozen

Product Specific References

References for CD4 Alpha antibody

  1. Saalmüller A et al. (2001) Summary of workshop findings for porcine T-lymphocyte-specific monoclonal antibodies.
    Vet Immunol Immunopathol. 80 (1-2): 35-52.
  2. Castellano, G. et al. (2010) Therapeutic targeting of classical and lectin pathways of complement protects from ischemia-reperfusion-induced renal damage.
    Am J Pathol. 176: 1648-59.
  3. Inman, C.F. et al. (2010) Dendritic cells interact with CD4 T cells in intestinal mucosa.
    J Leukoc Biol. 88 (3): 571-8.
  4. Kick AR et al. (2011) Evaluation of peripheral lymphocytes after weaning and vaccination for Mycoplasma hyopneumoniae.
    Res Vet Sci. 91 (3): e68-72.
  5. Kick, A.R. et al. (2012) Effects of stress associated with weaning on the adaptive immune system in pigs.
    J Anim Sci. 90: 649-56.
  6. Goujon, J.M. et al. (2000) Influence of cold-storage conditions on renal function of autotransplanted large pig kidneys.
    Kidney Int. 58: 838-50.
  7. Tambuyzer BR et al. (2012) Osteopontin alters the functional profile of porcine microglia in vitro.
    Cell Biol Int. 36 (12): 1233-8.
  8. Tuchscherer, M. et al. (2012) Effects of inadequate maternal dietary protein:carbohydrate ratios during pregnancy on offspring immunity in pigs.
    BMC Vet Res. 8: 232.
  9. Cao, D. et al. (2010) Synthetic innate defence regulator peptide enhances in vivo immunostimulatory effects of CpG-ODN in newborn piglets.
    Vaccine. 28: 6006-13.
  10. Clapperton, M. et al. (2005) Associations of weight gain and food intake with leukocyte sub-sets in Large White pigs
    Livestock Production Science 96: 249-60
  11. Clapperton, M. et al. (2005) Innate immune traits differ between Meishan and Large White pigs.
    Vet Immunol Immunopathol. 104: 131-44.
  12. Clapperton, M. et al. (2008) Pig peripheral blood mononuclear leucocyte subsets are heritable and genetically correlated with performance.
    Animal. 2: 1575-84.
  13. Faure, J.P. et al. (2002) Polyethylene glycol reduces early and long-term cold ischemia-reperfusion and renal medulla injury.
    J Pharmacol Exp Ther. 2002 Sep;302(3):861-70.
  14. Faure, J.P. et al. (2004) Evidence for protective roles of polyethylene glycol plus high sodium solution and trimetazidine against consequences of renal medulla ischaemia during cold preservation and reperfusion in a pig kidney model.
    Nephrol Dial Transplant. 19: 1742-51.
  15. Inman, C.F. et al. (2012) Neonatal colonisation expands a specific intestinal antigen-presenting cell subset prior to CD4 T-cell expansion, without altering T-cell repertoire.
    PLoS One. 7(3): e33707.
  16. Kick, A.R. et al. (2012) Effects of stress associated with weaning on the adaptive immune system in pigs.
    J Anim Sci. 90: 649-56.
  17. Langerhuus, S.N. et al. (2010) Brief report: biomarkers of aortic vascular prosthetic graft infection in a porcine model with Staphylococcus aureus.
    Eur J Clin Microbiol Infect Dis. 29: 1453-6.
  18. Lu, X. et al. (2012) Genome-wide association study for T lymphocyte subpopulations in swine.
    BMC Genomics. 13: 488.
  19. Monroy-Salazar, H.G. et al. (2012) Effects of a live yeast dietary supplement on fecal coliform counts and on peripheral blood CD4+ and CD8+ lymphocyte subpopulations in nursery pigs.
    J Swine Health Prod 20: 276-282.
  20. Shi, K. et al. (2008) Changes in peripheral blood leukocyte subpopulations in piglets co-infected experimentally with porcine reproductive and respiratory syndrome virus and porcine circovirus type 2.
    Vet Microbiol. 129: 367-77.
  21. Spreeuwenberg, M.A. et al. (2001) Small intestine epithelial barrier function is compromised in pigs with low feed intake at weaning.
    J Nutr. 131: 1520-7.
  22. Tambuyzer, B.R. et al. (2012) Osteopontin alters the functional profile of porcine microglia in vitro.
    Cell Biol Int. 36: 1233-8.
  23. Zelnickova, P. et al. (2007) Intracellular cytokine detection by flow cytometry in pigs: fixation, permeabilization and cell surface staining.
    J Immunol Methods. 327: 18-29.
  24. Kvist, P.H. et al. (2010) Effect of subcutaneous glucose sensor implantation on skin mRNA expression in pigs.
    Diabetes Technol Ther. 12: 791-9.
  25. Lefevre, E.A. et al. (2012) Immune responses in pigs vaccinated with adjuvanted and non-adjuvanted A(H1N1)pdm/09 influenza vaccines used in human immunization programmes.
    PLoS One. 7(3): e32400.
  26. Akershoek, J.J. et al. (2016) Cell therapy for full-thickness wounds: are fetal dermal cells a potential source?
    Cell Tissue Res. 364 (1): 83-94.
  27. Liu J et al. (2016) The Role of Porcine Monocyte Derived Dendritic Cells (MoDC) in the Inflammation Storm Caused by Streptococcus suis Serotype 2 Infection.
    PLoS One. 11 (3): e0151256.
  28. Liermann, W. et al. (2016) Effects of two commercial diets and technical feed treatment on stomach lesions and immune system of fattening pigs.
    J Anim Physiol Anim Nutr (Berl). Nov 2. [Epub ahead of print]
  29. Gardner, D.S. et al. (2016) Remote effects of acute kidney injury in a porcine model.
    Am J Physiol Renal Physiol. 310 (4): F259-71.
  30. Hemmink, J.D. et al. (2016) Distinct immune responses and virus shedding in pigs following aerosol, intra-nasal and contact infection with pandemic swine influenza A virus, A(H1N1)09.
    Vet Res. 47 (1): 103.
  31. Dąbrowski, M. et al. (2017) The Effect of Deoxynivalenol on Selected Populations of Immunocompetent Cells in Porcine Blood-A Preliminary Study.
    Molecules. 22 (5)Apr 26 [Epub ahead of print].
  32. Hsu, W.T. et al. (2013) Prostaglandin E2 potentiates mesenchymal stem cell-induced IL-10+IFN-γ+CD4+ regulatory T cells to control transplant arteriosclerosis.
    J Immunol. 190 (5): 2372-80.
  33. López, E. et al. (2019) Identification of very early inflammatory markers in a porcine myocardial infarction model.
    BMC Vet Res. 15 (1): 91.
  34. Hu, Z. et al. (2019) Genomic variant in porcine TNFRSF1A gene and its effects on TNF signaling pathway in vitro.
    Gene. 700: 105-9.
  35. Fogle, J.E. et al. (2019) Antibiotic Therapy Does Not Alter the Humoral Response to Vaccination for Porcine Circovirus 2 in Weaned Pigs.
    Vet Sci. 6 (2) May 30 [Epub ahead of print].

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.
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