CD4 Alpha antibody | MIL17








Mouse anti Pig CD4 Alpha:FITC
Mouse anti Pig CD4 Alpha
Mouse anti Pig CD4 Alpha:RPE
- Product Type
- Monoclonal Antibody
- Clone
- MIL17
- Isotype
- IgG2b
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
MCA1749F | F | 0.1 mg |
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MCA1749GA | C* F | 0.1 mg |
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MCA1749PE | F | 100 Tests |
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Product Details
- Target Species
- Pig
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of CD4 Alpha antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | ||
Flow Cytometry | 1/25 | 1/200 | |
Immunohistology - Frozen 1 | |||
Immunohistology - Paraffin | |||
Flow Cytometry | Neat |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG2b Negative Control:FITC | MCA691F | F | 100 Tests |
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Mouse IgG2b Negative Control | MCA691 | F | 100 Tests |
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Mouse IgG2b Negative Control:RPE | MCA691PE | F | 100 Tests |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse anti Pig CD45 | MCA1222GA | C F IF | 0.1 mg |
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Mouse anti Pig wCD8 Alpha | MCA1223GA | C F | 0.1 mg |
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Rat anti Human CD3 | MCA1477 | C F* IF P* WB | 0.2 mg |
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Product Specific References
References for CD4 Alpha antibody
-
Saalmüller A et al. (2001) Summary of workshop findings for porcine T-lymphocyte-specific monoclonal antibodies.
Vet Immunol Immunopathol. 80 (1-2): 35-52. -
Castellano, G. et al. (2010) Therapeutic targeting of classical and lectin pathways of complement protects from ischemia-reperfusion-induced renal damage.
Am J Pathol. 176: 1648-59. -
Inman, C.F. et al. (2010) Dendritic cells interact with CD4 T cells in intestinal mucosa.
J Leukoc Biol. 88 (3): 571-8. -
Kick AR et al. (2011) Evaluation of peripheral lymphocytes after weaning and vaccination for Mycoplasma hyopneumoniae.
Res Vet Sci. 91 (3): e68-72. -
Kick, A.R. et al. (2012) Effects of stress associated with weaning on the adaptive immune system in pigs.
J Anim Sci. 90: 649-56. -
Goujon, J.M. et al. (2000) Influence of cold-storage conditions on renal function of autotransplanted large pig kidneys.
Kidney Int. 58: 838-50. -
Tambuyzer BR et al. (2012) Osteopontin alters the functional profile of porcine microglia in vitro.
Cell Biol Int. 36 (12): 1233-8. -
Tuchscherer, M. et al. (2012) Effects of inadequate maternal dietary protein:carbohydrate ratios during pregnancy on offspring immunity in pigs.
BMC Vet Res. 8: 232. -
Cao, D. et al. (2010) Synthetic innate defence regulator peptide enhances in vivo immunostimulatory effects of CpG-ODN in newborn piglets.
Vaccine. 28: 6006-13. -
Clapperton, M. et al. (2005) Associations of weight gain and food intake with leukocyte sub-sets in Large White pigs
Livestock Production Science 96: 249-60 -
Clapperton, M. et al. (2005) Innate immune traits differ between Meishan and Large White pigs.
Vet Immunol Immunopathol. 104: 131-44. -
Clapperton, M. et al. (2008) Pig peripheral blood mononuclear leucocyte subsets are heritable and genetically correlated with performance.
Animal. 2: 1575-84. -
Faure, J.P. et al. (2002) Polyethylene glycol reduces early and long-term cold ischemia-reperfusion and renal medulla injury.
J Pharmacol Exp Ther. 2002 Sep;302(3):861-70. -
Faure, J.P. et al. (2004) Evidence for protective roles of polyethylene glycol plus high sodium solution and trimetazidine against consequences of renal medulla ischaemia during cold preservation and reperfusion in a pig kidney model.
Nephrol Dial Transplant. 19: 1742-51. -
Inman, C.F. et al. (2012) Neonatal colonisation expands a specific intestinal antigen-presenting cell subset prior to CD4 T-cell expansion, without altering T-cell repertoire.
PLoS One. 7(3): e33707. -
Kick, A.R. et al. (2012) Effects of stress associated with weaning on the adaptive immune system in pigs.
J Anim Sci. 90: 649-56. -
Langerhuus, S.N. et al. (2010) Brief report: biomarkers of aortic vascular prosthetic graft infection in a porcine model with Staphylococcus aureus.
Eur J Clin Microbiol Infect Dis. 29: 1453-6. -
Lu, X. et al. (2012) Genome-wide association study for T lymphocyte subpopulations in swine.
BMC Genomics. 13: 488. -
Monroy-Salazar, H.G. et al. (2012) Effects of a live yeast dietary supplement on fecal coliform counts and on peripheral blood CD4+ and CD8+ lymphocyte subpopulations in nursery pigs.
J Swine Health Prod 20: 276-282. -
Shi, K. et al. (2008) Changes in peripheral blood leukocyte subpopulations in piglets co-infected experimentally with porcine reproductive and respiratory syndrome virus and porcine circovirus type 2.
Vet Microbiol. 129: 367-77. -
Spreeuwenberg, M.A. et al. (2001) Small intestine epithelial barrier function is compromised in pigs with low feed intake at weaning.
J Nutr. 131: 1520-7. -
Tambuyzer, B.R. et al. (2012) Osteopontin alters the functional profile of porcine microglia in vitro.
Cell Biol Int. 36: 1233-8. -
Zelnickova, P. et al. (2007) Intracellular cytokine detection by flow cytometry in pigs: fixation, permeabilization and cell surface staining.
J Immunol Methods. 327: 18-29. -
Kvist, P.H. et al. (2010) Effect of subcutaneous glucose sensor implantation on skin mRNA expression in pigs.
Diabetes Technol Ther. 12: 791-9. -
Lefevre, E.A. et al. (2012) Immune responses in pigs vaccinated with adjuvanted and non-adjuvanted A(H1N1)pdm/09 influenza vaccines used in human immunization programmes.
PLoS One. 7(3): e32400. -
Akershoek, J.J. et al. (2016) Cell therapy for full-thickness wounds: are fetal dermal cells a potential source?
Cell Tissue Res. 364 (1): 83-94. -
Liu J et al. (2016) The Role of Porcine Monocyte Derived Dendritic Cells (MoDC) in the Inflammation Storm Caused by Streptococcus suis Serotype 2 Infection.
PLoS One. 11 (3): e0151256. -
Liermann, W. et al. (2017) Effects of two commercial diets and technical feed treatment on stomach lesions and immune system of fattening pigs.
J Anim Physiol Anim Nutr (Berl). 101 (5): e414-26. -
Gardner, D.S. et al. (2016) Remote effects of acute kidney injury in a porcine model.
Am J Physiol Renal Physiol. 310 (4): F259-71. -
Hemmink, J.D. et al. (2016) Distinct immune responses and virus shedding in pigs following aerosol, intra-nasal and contact infection with pandemic swine influenza A virus, A(H1N1)09.
Vet Res. 47 (1): 103. -
Dąbrowski, M. et al. (2017) The Effect of Deoxynivalenol on Selected Populations of Immunocompetent Cells in Porcine Blood-A Preliminary Study.
Molecules. 22 (5)Apr 26 [Epub ahead of print]. -
Hsu, W.T. et al. (2013) Prostaglandin E2 potentiates mesenchymal stem cell-induced IL-10+IFN-γ+CD4+ regulatory T cells to control transplant arteriosclerosis.
J Immunol. 190 (5): 2372-80. -
López, E. et al. (2019) Identification of very early inflammatory markers in a porcine myocardial infarction model.
BMC Vet Res. 15 (1): 91. -
Hu, Z. et al. (2019) Genomic variant in porcine TNFRSF1A gene and its effects on TNF signaling pathway in vitro.
Gene. 700: 105-9. -
Fogle, J.E. et al. (2019) Antibiotic Therapy Does Not Alter the Humoral Response to Vaccination for Porcine Circovirus 2 in Weaned Pigs.
Vet Sci. 6 (2)May 30 [Epub ahead of print].
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
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