CD335 antibody | VIV-KM1













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F IF ResetMouse anti Pig CD335
- Product Type
- Monoclonal Antibody
- Clone
- VIV-KM1
- Isotype
- IgG1
- Specificity
- CD335
Mouse anti Pig CD335 antibody, clone VIV-KM1 recognizes the porcine homologue of human CD335, also known as NKp46 and natural cytotoxicity triggering receptor 1 (NCR1), a member of the natural cytotoxicity receptor (NCR) family. CD335 is a type I transmembrane protein, with two extracellular C2-type immunoglobulin-like domains, which functions as an activating receptor and is involved in the control of viral infection and tumor development. CD335 is expressed by human natural killer cells (Sivori et al.1997). The development of monoclonal antibodies to bovine CD335 (clone AKS1) (Storset et al. 2004) and ovine CD335 (clone EC1.1) (Connelley et al.2011) have enabled researchers to identify and better understand ruminant NK cells. Mouse anti Pig CD335 antibody, clone VIV-KM is the first monoclonal developed to specifically identify porcine CD335 and provides a reagent to facilitate a better understanding of the pig immune system and aid in the understanding of the role of NK cells in host pathogen defense. Porcine CD335 is not expressed by all NK cells and expression may be influenced by cytokine production (Mair et al. 2012). |
- Target Species
- Pig
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Fusion protein consisting of the extracellular region of porcine CD335
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | ![]() |
1/50 | 1/200 |
Immunofluorescence | ![]() |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Mouse anti Pig CD25 | MCA1736GA | C F | 0.1 mg |
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Description | Mouse anti Pig CD25 | ||||||
Mouse anti Pig CD107a:Alexa Fluor® 647 | MCA2315A647 | F* | 100 Tests/1ml |
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Description | Mouse anti Pig CD107a:Alexa Fluor® 647 | ||||||
Mouse anti Pig CD107a:FITC | MCA2315F | F* | 0.1 mg |
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Description | Mouse anti Pig CD107a:FITC | ||||||
Mouse anti Pig CD3:FITC | MCA5951F | F | 0.1 mg |
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Description | Mouse anti Pig CD3:FITC | ||||||
Mouse anti Pig CD3:RPE | MCA5951PE | F | 100 Tests |
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Description | Mouse anti Pig CD3:RPE |
References for CD335 antibody
-
Forberg, H. et al. (2014) Early responses of natural killer cells in pigs experimentally infected with 2009 pandemic H1N1 influenza A virus.
PLoS One. 9 (6): e100619. -
Mair, K.H. et al. (2013) Porcine CD8αdim/-NKp46high NK cells are in a highly activated state.
Vet Res. 44: 13. -
Mair, K.H. et al. (2012) NKp46 expression discriminates porcine NK cells with different functional properties.
Eur J Immunol. 42 (5): 1261-71. -
Yang, G. et al. (2017) Characterizing porcine invariant natural killer T cells: A comparative study with NK cells and T cells.
Dev Comp Immunol. 76: 343-51.
Further Reading
-
Connelley, T. et al. (2011) NKp46 defines ovine cells that have characteristics corresponding to NK cells.
Vet Res. 42: 37. -
Storset, A.K. et al. (2004) NKp46 defines a subset of bovine leukocytes with natural killer cell characteristics.
Eur J Immunol. 34 (3): 669-76. -
Sivori, S. et al. (1997) p46, a novel natural killer cell-specific surface molecule that mediates cell activation.
J Exp Med. 186 (7): 1129-36.
- Synonyms
- NKp46
MCA5972GA



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