CD335 antibody | VIV-KM1

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Mouse anti Pig CD335:Alexa Fluor® 488

Mouse anti Pig CD335:APC

Mouse anti Pig CD335

Product Type
Monoclonal Antibody
Clone
VIV-KM1
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA5972A488 F 100 Tests/1ml
MCA5972APC F 100 Tests
MCA5972GA F IF 0.1 mg
Mouse anti Pig CD335 antibody, clone VIV-KM1 recognizes the porcine homologue of human CD335, also known as NKp46 and natural cytotoxicity triggering receptor 1 (NCR1), a member of the natural cytotoxicity receptor (NCR) family.

CD335 is a type I transmembrane protein, with two extracellular C2-type immunoglobulin-like domains, which functions as an activating receptor and is involved in the control of viral infection and tumor development.

Until recently little has been known about porcine and veterinary NK cells. CD335 is expressed by human natural killer cells (Sivori, S. et al.1997) and the development of monoclonal antibodies to bovine CD335 (clone ASK1) (Storset et al. 2004) and ovine CD335 (clone EC1.1) (Connelley et al.2011) have enabled researchers to identify and better understand ruminant NK cells. Clone VIV-KM1 is the first monoclonal developed to specifically identify porcine CD335 and provides a reagent to facilitate a better understanding of the pig immune system and aid in the understanding of the role of NK cells in host pathogen defence. Studies using VIV-KM1 have shown that, within the pig, CD335 is not universally expressed by all NK cells and that expression of this marker on NK cells may be influenced by cytokine production (Mair et al. 2012).

In addition to clone VIV-KM1, clones AKS1, which recognizes CD335 (NKp46) in bovine and other ruminants, and EC1.1, which recognizes ovine and caprine CD335, are also available from Bio-Rad.

Product Details

Target Species
Pig
Product Form
Purified IgG conjugated to Alexa Fluor® 488 - liquid
Product Form
Purified IgG conjugated to Allophycocyanin (APC) - lyophilised
Product Form
Purified IgG - liquid
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Fusion protein consisting of the extracellular region of porcine CD335
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0 myeloma cell line

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Shelf Life
18 months from date of despatch
Shelf Life
12 months from date of reconstitution
Shelf Life
18 months from date of despatch.

More Information

Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD335 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry Neat
Flow Cytometry 1/50 1/200
Immunofluorescence
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system to a concentration equivalent to their test reagent.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 488 MCA928A488 100 Tests/1ml F
Mouse IgG1 Negative Control:APC MCA928APC 100 Tests F
Mouse IgG1 Negative Control MCA928 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti Pig CD16:RPE MCA1971PE 100 Tests F
Mouse anti Bovine CD335 MCA2365GA 0.1 mg F IF WB
Mouse anti Pig CD3:RPE MCA5951PE 100 Tests F
Mouse anti Pig CD27:RPE MCA5973PE 100 Tests F
Human anti Pig Interferon Gamma HCA090 0.1 mg E
Mouse anti Pig CD45:FITC MCA1222F 100 Tests F
Mouse anti Pig wCD8 Alpha:RPE MCA1223PE 100 Tests F
Rat anti Human CD3:Alexa Fluor®647 MCA1477A647 100 Tests/1ml F*
Rat anti Human CD3:Pacific Blue® MCA1477PB 100 Tests/1ml F*
Mouse anti Pig CD25 MCA1736GA 0.1 mg C F
Mouse anti Pig CD107a:Alexa Fluor® 647 MCA2315A647 100 Tests/1ml F*
Mouse anti Pig CD107a:FITC MCA2315F 0.1 mg F*
Mouse anti Pig CD3:FITC MCA5951F 0.1 mg F
Mouse anti Pig CD3:RPE MCA5951PE 100 Tests F

Product Specific References

References for CD335 antibody

  1. Forberg, H. et al. (2014) Early responses of natural killer cells in pigs experimentally infected with 2009 pandemic H1N1 influenza A virus.
    PLoS One. 9 (6): e100619.
  2. Mair, K.H. et al. (2013) Porcine CD8αdim/-NKp46high NK cells are in a highly activated state.
    Vet Res. 44: 13.
  3. Mair, K.H. et al. (2012) NKp46 expression discriminates porcine NK cells with different functional properties.
    Eur J Immunol. 42 (5): 1261-71.

Further Reading

  1. Connelley, T. et al. (2011) NKp46 defines ovine cells that have characteristics corresponding to NK cells.
    Vet Res. 42: 37.
  2. Storset, A.K. et al. (2004) NKp46 defines a subset of bovine leukocytes with natural killer cell characteristics.
    Eur J Immunol. 34 (3): 669-76.
  3. Sivori, S. et al. (1997) p46, a novel natural killer cell-specific surface molecule that mediates cell activation.
    J Exp Med. 186 (7): 1129-36.