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CD3 antibody | PPT3

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Anti Pig CD3 Antibody, clone PPT3 thumbnail image 1 Anti Pig CD3 Antibody, clone PPT3 thumbnail image 2 Anti Pig CD3 Antibody, clone PPT3 thumbnail image 3 Anti Pig CD3 Antibody, clone PPT3 thumbnail image 4 Anti Pig CD3 Antibody, clone PPT3 thumbnail image 5 Anti Pig CD3 Antibody, clone PPT3 thumbnail image 6 Anti Pig CD3 Antibody, clone PPT3 thumbnail image 7 Anti Pig CD3 Antibody, clone PPT3 thumbnail image 8
Anti Pig CD3 Antibody, clone PPT3 gallery image 1
Figure A. Alexa Fluor 647conjugated mouse anti porcine CD45 (MCA1222A647) and FITC conjugated mouse IgG1 isotype control (MCA928F). Figure B. Alexa Fluor 647 conjugated mouse anti porcine CD45 (MCA1222A647) and FITC conjugated mouse anti porcine CD3 (MCA5951F). All experiments performed on red cell lysed porcine blood gated on lymphocytes. Data acquired on the ZE5 Cell Analyzer.
Anti Pig CD3 Antibody, clone PPT3 gallery image 2
Figure A. APC conjugated mouse anti porcine CD27 (MCA5973APC) and RPE conjugated mouse IgG1 isotype control (MCA928PE). Figure B. APC conjugated mouse anti porcine CD27 (MCA5973APC) and RPE conjugated mouse anti porcine CD3 (MCA5951PE). All experiments performed on red cell lysed porcine blood gated on lymphocytes. Data acquired on the ZE5 Cell Analyzer.
Anti Pig CD3 Antibody, clone PPT3 gallery image 3
Staining of porcine peripheral blood lymphocytes with Mouse anti Pig CD3 (MCA5951EL) followed by Goat anti Mouse IgG (H/L):FITC (STAR117F).
Anti Pig CD3 Antibody, clone PPT3 gallery image 4
Staining of porcine peripheral blood lymphocytes with Mouse anti Pig CD3 (MCA5951GA) followed by Goat anti Mouse IgG (H/L):FITC (STAR117F).
Anti Pig CD3 Antibody, clone PPT3 gallery image 5
Figure A. FITC conjugated Mouse anti Porcine CD3 antibody, clone PPT3(MCA5951F) and purified mouse IgM isotype control (MCA692) detected with goat anti mouse IgM PE (STAR86PE). Figure B. FITC conjugated mouse anti porcine CD3 (MCA5951F) and purified mouse anti porcine CD45RC (MCA1750) detected with goat anti mouse IgM (STAR86PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Anti Pig CD3 Antibody, clone PPT3 gallery image 6
Figure A. Purified Mouse anti Porcine CD45RC (MCA1750) detected with Goat anti Mouse IgM PE (STAR86PE) and Mouse IgG1 FITC isotype control (MCA928F). Figure B. Purified Mouse anti Porcine CD45RC (MCA1750) detected with Goat anti Mouse IgM PE (STAR86PE) and Mouse anti Porcine CD3 FITC (MCA5951F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Anti Pig CD3 Antibody, clone PPT3 gallery image 7
Figure A. StarBright Violet 475 conjugated Mouse anti Porcine CD3 (​MCA5951SBV475). Figure B. StarBright Violet 475 conjugated Mouse anti Porcine CD3 (​MCA5951SBV475) and StarBright Violet 790 conjugated  Mouse anti Porcine CD16 (​MCA1971SBV790). All experiments performed on red cell lysed porcine blood gated on live single lymphocytes, in the presence of 10% porcine serum. Data acquired on the ZE5 Cell Analyzer.
Anti Pig CD3 Antibody, clone PPT3 gallery image 8
Figure A. StarBright UltraViolet 400 conjugated Mouse anti Porcine wCD8a (​MCA1223SBUV400). Figure B. StarBright UltraViolet 400 conjugated Mouse anti Porcine wCD8a (​MCA1223SBUV400) and StarBright Violet 790 conjugated  Mouse anti Porcine CD3 (​MCA5951SBV790). All experiments performed on porcine PBMCs gated on live single lymphocytes, in the presence of 10% porcine serum. Data acquired on the ZE5 Cell Analyzer.

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Mouse anti Pig CD3:RPE

Product Type
Monoclonal Antibody
Clone
PPT3
Isotype
IgG1
Specificity
CD3
Region
EPSILON

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Product Code Applications Pack Size List Price Your Price Qty
MCA5951PE
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests loader
List Price Your Price
loader

Mouse anti Pig CD3, clone PPT3 recognizes the porcine homologue of human CD3ε, a 24 kDa single pass type I membrane protein expressed by T-lymphocytes. Clone PPT3, also known under the clone designation FY1H2, was clustered at the second international swine CD workshop and found to specifically recognise an epitope on the porcine CD3ε designated as CD3c (Pescovitz, M.D., et al. 1998).

CD3 is a multimeric protein complex composed of four distinct polypeptide chains (ε, γ, δ, ζ) that assemble and function as three pairs of dimers (εγ, εδ, ζζ). The CD3 complex serves as a T cell co-receptor that associates non-covalently with the T cell receptor (TCR) (Guy, C.S & Vignali, D.G. 2009). CD3 is a defining feature of cells belonging to the T cell lineage, antibodies recognising pig CD3 therefore provide useful markers of porcine T cells.

Clone PPT3 has been demonstrated to recognise an epitope that is expressed both intracellularly and extracellularly, additionally clone PPT3 has been demonstrated to activate α/β T-cells (Kirkham P.A., et al. 1996).

Clone PPT3 was tested on PBL from a range of other mammalian species and found to be negative suggesting that the epitope recognised by this clone is specific to porcine (Yang, H. et al. 1996).

Target Species
Pig
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
Goat
Horse
Human
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Immunogen
Porcine PBMCs
Fusion Partners
Lymph node cells from immunized BALB/c mice were fused with cells of the NS0 myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests
List Price Your Price
Description Mouse IgG1 Negative Control:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse anti Pig wCD8 Alpha:FITC MCA1223F F 0.1 mg loader
List Price Your Price
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Description Mouse anti Pig wCD8 Alpha:FITC
Mouse anti Pig CD25 MCA1736GA C F 0.1 mg loader
List Price Your Price
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Description Mouse anti Pig CD25
Mouse anti Pig CD4 Alpha:FITC MCA1749F F 0.1 mg loader
List Price Your Price
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Description Mouse anti Pig CD4 Alpha:FITC

Source Reference

  1. Yang, H. et al. (1996) Preparation of monoclonal anti-porcine CD3 antibodies and preliminary characterization of porcine T lymphocytes.
    Immunology. 88 (4): 577-85.

References for CD3 antibody

  1. Kirkham, P.A. et al. (1996) Porcine CD3 epsilon: its characterization, expression and involvement in activation of porcine T lymphocytes.
    Immunology. 87 (4): 616-23.
  2. Uehlein, S. et al. (2021) Human-like Response of Pig T Cells to Superagonistic Anti-CD28 Monoclonal Antibodies.
    J Immunol. 207 (10): 2473-88.
  3. Zhao, H. et al. (2022) Development of RAG2 -/- IL2Rγ -/Y immune deficient FAH-knockout miniature pig.
    Front Immunol. 13: 950194.
  4. Maciag, S.S. et al. (2022) On the influence of the source of porcine colostrum in the development of early immune ontogeny in piglets.
    Sci Rep. 12 (1): 15630.
  5. dos Santos, M.C. et al. (2023) Effect of yeast extracted β-glucans on the immune response and reproductive performance of gilts in the adaptation, gestation, and lactation periods
    Livestock Science. 275: 105289.
  6. Haach, V. et al. (2023) A polyvalent virosomal influenza vaccine induces broad cellular and humoral immunity in pigs.
    Virol J. 20 (1): 181.
  7. Hu, Z. et al. (2019) Genomic variant in porcine TNFRSF1A gene and its effects on TNF signaling pathway in vitro.
    Gene. 700: 105-109.
  8. Boschetto, F. et al. (2024) Protocol for extracting and isolating porcine bone-marrow-derived macrophages from ribs.
    STAR Protoc. 5 (2): 103085.
    9. View The Latest Product References
  9. Maciag, S. et al. (2022) Effects of freezing storage on the stability of maternal cellular and humoral immune components in porcine colostrum.
    Vet Immunol Immunopathol. 254: 110520.
  10. Forner, R. et al. (2021) Distribution difference of colostrum-derived B and T cells subsets in gilts and sows.
    PLoS One. 16 (5): e0249366.

Further Reading

  1. Guy, C.S. & Vignali, D.A. (2009) Organization of proximal signal initiation at the TCR:CD3 complex.
    Immunol Rev. 32: 7-21.

UniProt
Q7YRN2
Entrez Gene
CD3E
GO Terms
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity

MCA5951PE

155390 166382 INN1710

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