CD3 antibody | PPT3

Mouse anti Pig CD3:RPE

Product Type
Monoclonal Antibody
Clone
PPT3
Isotype
IgG1
Specificity
CD3
Region
EPSILON

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MCA5951PE
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Mouse anti Pig CD3, clone PPT3 recognizes the porcine homologue of human CD3ε, a 24 kDa single pass type I membrane protein expressed by T-lymphocytes. Clone PPT3, also known under the clone designation FY1H2, was clustered at the second international swine CD workshop and found to specifically recognise an epitope on the porcine CD3ε designated as CD3c (Pescovitz, M.D., et al. 1998).

CD3 is a multimeric protein complex composed of four distinct polypeptide chains (ε, γ, δ, ζ) that assemble and function as three pairs of dimers (εγ, εδ, ζζ). The CD3 complex serves as a T cell co-receptor that associates non-covalently with the T cell receptor (TCR) (Guy, C.S & Vignali, D.G. 2009). CD3 is a defining feature of cells belonging to the T cell lineage, antibodies recognising pig CD3 therefore provide useful markers of porcine T cells.

Clone PPT3 has been demonstrated to recognise an epitope that is expressed both intracellularly and extracellularly, additionally clone PPT3 has been demonstrated to activate α/β T-cells (Kirkham P.A., et al. 1996).

Clone PPT3 was tested on PBL from a range of other mammalian species and found to be negative suggesting that the epitope recognised by this clone is specific to porcine (Yang, H. et al. 1996).

Target Species
Pig
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
Goat
Horse
Human
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Immunogen
Porcine PBMCs
Fusion Partners
Lymph node cells from immunised BALB/c mice were fused with cells of the NS0 myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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References for CD3 antibody

  1. Yang, H. et al. (1996) Preparation of monoclonal anti-porcine CD3 antibodies and preliminary characterization of porcine T lymphocytes.
    Immunology. 88 (4): 577-85.
  2. Kirkham, P.A. et al. (1996) Porcine CD3 epsilon: its characterization, expression and involvement in activation of porcine T lymphocytes.
    Immunology. 87 (4): 616-23.
  3. Pescovitz, M.D. et al. (1998) Analyses of monoclonal antibodies reacting with porcine CD3: results from the Second International Swine CD Workshop.
    Vet Immunol Immunopathol. 60: 261-8.
  4. Forberg H et al. (2014) Early responses of natural killer cells in pigs experimentally infected with 2009 pandemic H1N1 influenza A virus.
    PLoS One. 9 (6): e100619.
  5. Uehlein, S. et al. (2021) Human-like Response of Pig T Cells to Superagonistic Anti-CD28 Monoclonal Antibodies.
    J Immunol. Oct 08 [Epub ahead of print].
  6. Zhao, H. et al. (2022) Development of RAG2 -/- IL2Rγ -/Y immune deficient FAH-knockout miniature pig.
    Front Immunol. 13: 950194.

Further Reading

  1. Guy, C.S. & Vignali, D.A. (2009) Organization of proximal signal initiation at the TCR:CD3 complex.
    Immunol Rev. 32: 7-21.
  2. Maciag, S.S. et al. (2022) The influence of source of porcine colostrum in development of early immune ontogeny in the piglet
    Ref Sq. Mar 24 [Epub ahead of print].

UniProt
Q7YRN2
Entrez Gene
CD3E
GO Terms
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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