Huntingtin antibody | HDC8A4
Mouse anti Huntingtin antibody, clone HDC8A4 (MCA2051) used to demonstrate perinuclear huntingtin expression and it's co-localization with the endoplasmic reticulum by immunofluorescence.
Image caption:
Confocal microscopy experiments showing that HTT proteins are enriched in the perinuclear and co-localize with the endoplasmic reticulum (ER) marker calreticulin upon treatment of Rp-cAMP or 8-pCpt2'-O-Me-cAMP (8-pCpt-cAMP). upper panels representative images showing the immunofluorescent signals of Htt (green), ER marker (red, only in the third and fourth columns) and DAPI (blue) in STHdh Q7/Q11 cells treated by vehicle, 1 μM Rp-cAMP or 1 μM 8-pCpt-cAMP. Scale bars, 20 μM. The two panels on the right side are magnified images from the left for visualizing the co-localization. Yellow pixels indicate co-localization.
From: Yao Y, Cui X, Al-Ramahi I, Sun X, Li B, Hou J, Difiglia M, Palacino J, Wu ZY, Ma L, Botas J, Lu B. A striatal-enriched intronic GPCR modulates huntingtin levels and toxicity. Elife. 2015 Mar 4;4.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Huntingtin antibody, clone HDC8A4 (MCA2051) used to demonstrate perinuclear huntingtin expression and it's co-localization with the endoplasmic reticulum by immunofluorescence.
Image caption:
Representative confocal microscopic immunofluorescent images of Htt and Rab39B in STHdhQ7/Q11cells. Treatment with 1 μM RP-cAMP or 1 μM 8-pCPT-2'-O-Me-cAMP for 48 hours leads to increased co-localization between Htt and Rab39B. Red: Rab39B; Blue: DAPI; Green: Htt (using antibody MCA2051); The lower panels are magnified images from the upper for visualizing the co-localization. Yellow pixels indicate co-localization. Scale bars: 20 μm. Right panels: co-localization parameters including Pearson's coefficient and overlap coefficient (mean and s.e.m.). Numbers indicate the number of cells analyzed for each treatment from 5 or more biological replicates.
From: Yao Y, Cui X, Al-Ramahi I, Sun X, Li B, Hou J, Difiglia M, Palacino J, Wu ZY, Ma L, Botas J, Lu B. A striatal-enriched intronic GPCR modulates huntingtin levels and toxicity. Elife. 2015 Mar 4;4.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Huntingtin antibody, clone HDC8A4 (MCA2051) used to demonstrate perinuclear huntingtin expression and it's co-localization with the endoplasmic reticulum by immunofluorescence.
Image caption:
No increase of Htt co-localization with Rab22A. Representative confocal microscopic immunofluorescent images of Htt and Rab22A in STHdhQ7/Q11 cells and quantitation analysis of co-localization. Treatments with 1 μM RP-cAMP or 1 μM 8-pCPT-2'-O-cAMP for 48 hours leads to no change of co-localization between Htt and Rab22A. Red Rab22A; Blue: DAPI; Green: Htt (using antibody MCA2051); Scale bars 20μm.
From: Yao Y, Cui X, Al-Ramahi I, Sun X, Li B, Hou J, Difiglia M, Palacino J, Wu ZY, Ma L, Botas J, Lu B. A striatal-enriched intronic GPCR modulates huntingtin levels and toxicity. Elife. 2015 Mar 4;4.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Huntingtin antibody, clone HDC8A4 (MCA2051) used to immunoprecipitate huntintin from mouse brain lysates.
Image caption:
Kalirin interacts with Htt in brain membranes. (a) and (b) Kalirin and Htt are mutually co-precipitated. Mouse brain membranes as described in Method were solubilized in lysis buffer containing 1% Triton X-100 and centrifuged to discard unsolubilized membranes. The supernatant was incubated with antibodies against Htt-C (aa2703-2911 of Htt)36, (a) or aa1641-1654 of kalirin-7 (b). After washes, immunoprecipitates were eluted into sample buffer and analyzed by SDS-PAGE and Western blot with indicated antibodies. Shown are blot analyses from one of four experiments for both (a) and (b).
From: McClory H, Wang X, Sapp E, Gatune LW, Iuliano M, Wu CY, Nathwani G, Kegel-Gleason KB, DiFiglia M, Li X.
The COOH-terminal domain of huntingtin interacts with RhoGEF kalirin and modulates cell survival.
Sci Rep. 2018 May 22;8(1):8000.
doi: 10.1038/s41598-018-26255-1
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Huntingtin antibody, clone HDC8A4 (MCA2051) used to detect huntintin from mouse brain lysates by western blotting..
Image caption:
The middle fragment of kalirin-7 (Klrn674-1272) interacts with Htt. Different regions of kalirin-7 were co-expressed with FLAG-tagged Htt2568-3144 in 293 T cells and enriched using Ni-NTA resins. Proteins on Ni-NTA resins were eluted into sample buffer and analyzed by SDS-PAGE and Western blot with an antibody against aa2703-2911 of Htt. Cell lysates for lower blots were run different gels and show the presence of transiently expressed huntingtin fragment in post nuclear supernatant used for the enrichment with Ni-NTA. Shown are data from one of three experiments.
From: McClory H, Wang X, Sapp E, Gatune LW, Iuliano M, Wu CY, Nathwani G, Kegel-Gleason KB, DiFiglia M, Li X.
The COOH-terminal domain of huntingtin interacts with RhoGEF kalirin and modulates cell survival.
Sci Rep. 2018 May 22;8(1):8000.
doi: 10.1038/s41598-018-26255-1
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
WB IF IP ResetMouse anti Huntingtin
- Product Type
- Monoclonal Antibody
- Clone
- HDC8A4
- Isotype
- IgG1
- Specificity
- Huntingtin
Antibody Quality and Performance Guarantee
| Mouse anti Huntingtin antibody, clone HDC8A4 reacts with an epitope corresponding to the HDC region (2703 - 2911 amino acids) of the huntingtin protein. Mouse anti Huntingtin antibody, clone HDC8A4 detects a ~350 kDa band on western blots but also detects smaller degradation products of huntingtin. Clone HDC8A4 recognizes both denatured and native huntingtin in human brain. The combined use of Mouse anti Huntingtin antibody, clone HDC8A4 (MCA2051) and HDB4E10 (MCA2050) demonstrate that huntingtin is enriched in neuronal cells in the brain. |
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rabbit Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Recombinant protein corresponding to amino acids 2703 - 2911 of huntingtin.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen 1 |  |
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| Immunoprecipitation | |
||
| Western Blotting | |
- 1Increased cytoplasmic staining, relative to nuclear, has been reported using formaldehyde as a fixative compared with acetone/methanol, see Wilkinson et al.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for Huntingtin antibody
-
Wilkinson, F. L. et al. (1999) Localization of rabbit huntingtin using a new panel of monoclonal antibodies.
Molecular Brain Research. 69: 10-20. -
Jones, A.L. (1999) The localization and interactions of huntingtin.
Philos Trans R Soc Lond B Biol Sci. 354 (1386): 1021-7. -
Li X et al. (2008) A function of huntingtin in guanine nucleotide exchange on Rab11.
Neuroreport. 19 (16): 1643-7. -
Lazzeroni G et al. (2013) A phenotypic screening assay for modulators of huntingtin-induced transcriptional dysregulation.
J Biomol Screen. 18 (9): 984-96. -
Yao Y et al. (2015) A striatal-enriched intronic GPCR modulates huntingtin levels and toxicity.
Elife. 4: 4. -
McClory, H. et al. (2018) The COOH-terminal domain of huntingtin interacts with RhoGEF kalirin and modulates cell survival.
Sci Rep. 8 (1): 8000.
- RRID
- AB_2123261
- UniProt
- P42858
- Entrez Gene
- HTT
- GO Terms
- GO:0000132 establishment of mitotic spindle orientation
- GO:0002039 p53 binding
- GO:0005829 cytosol
- GO:0005783 endoplasmic reticulum
- GO:0005624 membrane fraction
- GO:0005634 nucleus
- GO:0005770 late endosome
- GO:0005776 autophagic vacuole
- GO:0005794 Golgi apparatus
- View More GO Terms
- GO:0006890 retrograde vesicle-mediated transport, Golgi to ER
- GO:0007030 Golgi organization
- GO:0030424 axon
- GO:0030425 dendrite
- GO:0030659 cytoplasmic vesicle membrane
- GO:0034452 dynactin binding
- GO:0043234 protein complex
- GO:0045505 dynein intermediate chain binding
- GO:0047496 vesicle transport along microtubule
MCA2051
149348
161363
1709
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