Huntingtin Antibody | HDC8A4

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Huntingtin Antibody | HDC8A4 gallery image 1

Total protein extract of normal human cerebral cortex separated as a strip on a 3-12.5% gradient SDS-PAGE gel and Western blotted. The blot was probed with MCA2051

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Huntingtin Antibody | HDC8A4 gallery image 2

Published customer image:
Mouse anti Huntingtin antibody, clone HDC8A4 used to demonstrate perinuclear huntingtin expression and it's co-localization with the endoplasmic reticulum by immunofluorescence.
Image caption:
Confocal microscopy experiments showing that HTT proteins are enriched in the perinuclear and co-localize with the endoplasmic reticulum (ER) marker calreticulin upon treatment of Rp-cAMP or 8-pCpt2'-O-Me-cAMP (8-pCpt-cAMP). upper panels representative images showing the immunofluorescent signals of Htt (green), ER marker (red, only in the third and fourth columns) and DAPI (blue) in STHdh Q7/Q11 cells treated by vehicle, 1 μM Rp-cAMP or 1 μM 8-pCpt-cAMP. Scale bars, 20 μM. The two panels on the right side are magnified images from the left for visualizing the co-localization. Yellow pixels indicate co-localization.

From: Yao Y, Cui X, Al-Ramahi I, Sun X, Li B, Hou J, Difiglia M, Palacino J, Wu ZY, Ma L, Botas J, Lu B. A striatal-enriched intronic GPCR modulates huntingtin levels and toxicity. Elife. 2015 Mar 4;4.

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Huntingtin Antibody | HDC8A4 gallery image 3

Published customer image:
Mouse anti Huntingtin antibody, clone HDC8A4 used to demonstrate perinuclear huntingtin expression and it's co-localization with the endoplasmic reticulum by immunofluorescence.
Image caption:
Representative confocal microscopic immunofluorescent images of Htt and Rab39B in STHdhQ7/Q11cells. Treatment with 1 μM RP-cAMP or 1 μM 8-pCPT-2'-O-Me-cAMP for 48 hours leads to increased co-localization between Htt and Rab39B. Red: Rab39B; Blue: DAPI; Green: Htt (using antibody MCA2051); The lower panels are magnified images from the upper for visualizing the co-localization. Yellow pixels indicate co-localization. Scale bars: 20 μm. Right panels: co-localization parameters including Pearson's coefficient and overlap coefficient (mean and s.e.m.). Numbers indicate the number of cells analyzed for each treatment from 5 or more biological replicates.

From: Yao Y, Cui X, Al-Ramahi I, Sun X, Li B, Hou J, Difiglia M, Palacino J, Wu ZY, Ma L, Botas J, Lu B. A striatal-enriched intronic GPCR modulates huntingtin levels and toxicity. Elife. 2015 Mar 4;4.

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Huntingtin Antibody | HDC8A4 gallery image 4

Published customer image:
Mouse anti Huntingtin antibody, clone HDC8A4 used to demonstrate perinuclear huntingtin expression and it's co-localization with the endoplasmic reticulum by immunofluorescence.
Image caption:
No increase of Htt co-localization with Rab22A. Representative confocal microscopic immunofluorescent images of Htt and Rab22A in STHdhQ7/Q11 cells and quantitation analysis of co-localization. Treatments with 1 μM RP-cAMP or 1 μM 8-pCPT-2'-O-cAMP for 48 hours leads to no change of co-localization between Htt and Rab22A. Red Rab22A; Blue: DAPI; Green: Htt (using antibody MCA2051); Scale bars 20μm.

From: Yao Y, Cui X, Al-Ramahi I, Sun X, Li B, Hou J, Difiglia M, Palacino J, Wu ZY, Ma L, Botas J, Lu B. A striatal-enriched intronic GPCR modulates huntingtin levels and toxicity. Elife. 2015 Mar 4;4.

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  • Mouse anti Huntingtin
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    HDC8A4
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2051C *, IP, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA2051
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Huntingtin antibody, clone HDC8A4 reacts with an epitope corresponding to the HDC region (2703 - 2911 amino acids) of the huntingtin protein.

      Mouse anti Huntingtin antibody, clone HDC8A4 detects a 350KDa band on western blots but also detects smaller degradation products of huntingtin. Clone HDC8A4 recognises both denatured and native huntingtin in human brain.

      The combined use of Mouse anti Huntingtin antibody, clone HDC8A4 (MCA2051), HDB4E10 (MCA2050) and HDA3E10 (MCA2049) demonstrate that huntingtin is enriched in neuronal cells in the brain.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunoprecipitation
      Western Blotting
      Immunohistology - Frozen(1)
      (1)
      Increased cytoplasmic staining, relative to nuclear, has been reported using formaldehyde as a fixative compared with acetone/methanol, see Wilkinson et al.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Huntingtin Antibody Formats

    Formats Clone Applications Sizes available
    Huntingtin Antibody : Purified HDC8A4 C *, IP, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
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              References

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