Huntingtin Antibody | HDB4E10

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Huntingtin Antibody | HDB4E10 gallery image 1

Western Blot analysis of normal human cerebral cortex total protein extract run on a 3-12.5% gradient SDS-PAGE gel and probed with Mouse anti Huntingtin (MCA2050)

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Published customer image:
Mouse anti Huntingtin antibody, clone HDB4E10 used to demonstrate perinuclear huntingtin expression and it's co-localization with the endoplasmic reticulum by immunofluorescence.
Image caption:
Confocal microscopy experiments showing that HTT proteins are enriched in the perinuclear and co-localize with the endoplasmic reticulum (ER) marker calreticulin upon tratment of Rp-cAMP or 8-pCpt2'-O-Me-cAMP (8-pCpt-cAMP). upper panels representative images showing the immunofluorescent signals og Htt (green), ER marker (redonly in the third and fourth columns) and DAPI (blue) in STHdh Q7/Q11 cells treated by vehicle, iμM Rp-cAMP or 1μ 8-pCpt-cAMP. Scale bars, 20 μM. The two panels on the right side are magnified images from the left for visualizing the co-localization. Yellow pixels indicate co-localization.

From: Yao Y, Cui X, Al-Ramahi I, Sun X, Li B, Hou J, Difiglia M, Palacino J, Wu ZY, Ma L, Botas J, Lu B. A striatal-enriched intronic GPCR modulates huntingtin levels and toxicity. Elife. 2015 Mar 4;4.

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Mouse anti Huntingtin antibody, clone HDB4E10 used for the detection of huntingtin in recombinant mutant Htt samles by ELISA.
Image caption:
Full-length HTT-Q138 purification and standard curve for HTT-ELISA assay. Panel A, Gel coomassie staining of samples from the HTT-Q138 purification process (lane 1: molecular weight standard; lane 2: 30 µg RL1 cell lysate before induction; lane 3: 30 µg RL1 cell lysate 24 h post induction; lane 4: anti-FLAG affinity gel flow trough; lane 5–6: gel washes; lane 7–9: 5 µL of eluted fractions). Panel B, Western blot stained with anti-FLAG (lanes sequence as in panel A). Panel C, HTT-ELISA assay standard curve displays absorbance calibration values in duplicates individually. Curve showed is a representative example of multiple assays.

From: Massai L, Petricca L, Magnoni L, Rovetini L, Haider S, Andre R, Tabrizi SJ, Süssmuth SD, Landwehrmeyer BG, Caricasole A, Pollio G, Bernocco S. Development of an ELISA assay for the quantification of soluble huntingtin in human blood cells. BMC Biochem. 2013 Nov 25;14:34.

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Mouse anti Huntingtin antibody, clone HDB4E10 used for the detection of huntingtin in mammalian cell lysates by ELISA.
Image caption:
HTT-Q138 and HTT-Q17 quantification in mammalian cell lysates sample. Panel A, Fold-induction of HTT-Q138 expression detected with ELISA assay in uninduced and induced RL1 cells. Panel B, HTT-ELISA analysis of HEK293 cells transfected with full-length HTT-Q17 and HTT-Q138. Panel C and D, Western blot and densitometry analysis of HTT-Q17 and HTT-Q138 transfected HEK293 cells. The blot was probed with antibodies specific for HTT (HTT-H7540) and a-tubulin (loading control).

From: Massai L, Petricca L, Magnoni L, Rovetini L, Haider S, Andre R, Tabrizi SJ, Süssmuth SD, Landwehrmeyer BG, Caricasole A, Pollio G, Bernocco S. Development of an ELISA assay for the quantification of soluble huntingtin in human blood cells. BMC Biochem. 2013 Nov 25;14:34.

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Mouse anti Huntingtin antibody, clone HDB4E10 used for the detection of huntingtin in mammalian cell lysates and he effect of Hsp90 overexpression by ELISA.
Image caption:
Pharmacological assay validation. Panel A and B, Effect of HSP90 overexpression on HTT protein (by ELISA) and gene (by RT-PCR) respectively. Panel C and D, Effect of HSP90 inhibition by NVP-AUY922 on HTT protein (by ELISA) and gene (by RT-PCR) respectively. Readings are normalized against Ctrl or DMSO as appropriate; statistical analyses are performed with two-way ANOVA followed by Bonferroni test for multiple comparisons (* p?
From: Massai L, Petricca L, Magnoni L, Rovetini L, Haider S, Andre R, Tabrizi SJ, Süssmuth SD, Landwehrmeyer BG, Caricasole A, Pollio G, Bernocco S. Development of an ELISA assay for the quantification of soluble huntingtin in human blood cells. BMC Biochem. 2013 Nov 25;14:34.

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Huntingtin Antibody | HDB4E10 gallery image 6

Published customer image:
Mouse anti Huntingtin antibody, clone HDB4E10 used for the detection of huntingtin in Huntington disease patient blood cell lysates by ELISA.
Image caption:
HTT detection in blood cells sample. Quantification by HTT-ELISA on human PBMC total lysates from HD patients at different disease stages, premanifest and healthy volunteers controls (HV). Gene carrier samples were significantly different from healthy controls (one-way ANOVA followed by Bonferroni test for multiple comparisons wrt HV group, p?
From: Massai L, Petricca L, Magnoni L, Rovetini L, Haider S, Andre R, Tabrizi SJ, Süssmuth SD, Landwehrmeyer BG, Caricasole A, Pollio G, Bernocco S. Development of an ELISA assay for the quantification of soluble huntingtin in human blood cells. BMC Biochem. 2013 Nov 25;14:34.

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  • Mouse anti Huntingtin
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    HDB4E10
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2050C *, IP, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA2050
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Huntingtin antibody, clone HDB4E10 reacts with an epitope corresponding to the HDB region (amino acids 1844 - 2131) of the huntingtin protein.

      Mouse anti Huntingtin antibody, clone HDB4E10 detects a ~350 kDa band on western blots but also detects smaller degradation products of huntingtin. The combined use of Mouse anti Huntingtin antibody, clones HDB4E10 (MCA2050), HDC8A4 (MCA2051) and HDA3E10 (MCA2049) demonstrate that huntingtin is enriched in neuronal cells in the brain (Jones 1999).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunoprecipitation
      Western Blotting
      Immunohistology - Frozen(1)
      (1)
      Increased cytoplasmic staining, relative to nuclear, has been reported using formaldehyde as a fixative compared with acetone/methanol, see Wilkinson et al.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Brain
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Huntingtin Antibody Formats

    Formats Clone Applications Sizes available
    Huntingtin Antibody : Purified HDB4E10 C *, IP, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Brain
              • Application NameReference Images
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              References

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