PTEN Tumor Suppressor Protein antibody | A2b1

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Mouse anti PTEN Tumor Suppressor Protein

Product Type: Monoclonal Antibody
Clone: A2b1
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
OBT1120	C* IP WB			0.1 mg

Summary
Secondary Antibodies

Mouse anti PTEN tumour suppressor antibody, clone A2b1, is specific for PTEN, a tumour suppressor protein located on the human chromosome 10 that is often mutated in various types of advanced cancers.
PTEN exhibits protein phosphatase activity and can suppress the growth of glioma cells.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.1% Sodium Azide (NaN₃)

Immunogen

A 10 amino acid peptide corresponding to a region towards to the C-terminus of native PTEN

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P60484 Related reagents; O08586 Related reagents
Entrez Gene: Pten Related reagents; PTEN Related reagents
GO Terms: GO:0000287 magnesium ion binding; GO:0001525 angiogenesis; GO:0002902 regulation of B cell apoptosis; GO:0051726 regulation of cell cycle; GO:0006915 apoptosis; GO:0004438 phosphatidylinositol-3-phosphatase activity; GO:0004722 protein serine/threonine phosphatase activity; GO:0004725 protein tyrosine phosphatase activity; GO:0006470 protein dephosphorylation; GO:0006917 induction of apoptosis; GO:0007417 central nervous system development; GO:0008138 protein tyrosine/serine/threonine phosphatase activity; GO:0010975 regulation of neuron projection development; GO:0016314 phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity; GO:0016605 PML body; GO:0030165 PDZ domain binding; GO:0030336 negative regulation of cell migration; GO:0033032 regulation of myeloid cell apoptosis; GO:0043066 negative regulation of apoptosis; GO:0043542 endothelial cell migration; GO:0046855 inositol phosphate dephosphorylation; GO:0046856 phosphatidylinositol dephosphorylation; GO:0048738 cardiac muscle tissue development; GO:0050680 negative regulation of epithelial cell proliferation; GO:0051717 inositol-1,3,4,5-tetrakisphosphate 3-phosphatase activity; GO:0051800 phosphatidylinositol-3,4-bisphosphate 3-phosphatase activity; GO:0051895 negative regulation of focal adhesion assembly; GO:0051898 negative regulation of protein kinase B signaling cascade; GO:0060736 prostate gland growth; GO:0000079 regulation of cyclin-dependent protein kinase activity; GO:0001933 negative regulation of protein phosphorylation; GO:0005829 cytosol; GO:0008289 lipid binding; GO:0008283 cell proliferation; GO:0009898 internal side of plasma membrane; GO:0050821 protein stabilization; GO:0019899 enzyme binding; GO:0048015 phosphatidylinositol-mediated signaling; GO:0048011 nerve growth factor receptor signaling pathway; GO:0050852 T cell receptor signaling pathway; GO:0051091 positive regulation of transcription factor activity; GO:0060070 canonical Wnt receptor signaling pathway
Regulatory: For research purposes only

Applications of PTEN Tumor Suppressor Protein antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Immunohistology - Frozen ¹	1/20	1/50
Immunohistology - Paraffin
Immunoprecipitation	1/10	1/20
Western Blotting		1/100

¹The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Request a different product with the PTEN TUMOR SUPPRESSOR PROTEIN specificity