Perforin antibody | dG9

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Mouse anti Human Perforin

Mouse anti Human Perforin:RPE

Product Type
Monoclonal Antibody
Clone
dG9
Isotype
IgG2b
Product Code Applications Pack Size List Price Quantity
MCA6138
Datasheet
C F* IC IP P
MSDS
0.1 mg loader

MCA6138PE
Datasheet
F*
MSDS
100 Tests/1ml loader

Mouse anti Human perforin, clone dG9 recognizes perforin a 60-70 kDa glycoprotein largely expressed in CD8+ cytotoxin T lymphocytes and Natural Killer cells. It aids in defense against virus-infected or neoplastic cells by inducing apoptosis. Perforin achieves this by inserting a portion of its C-terminus region into the lipid membrane of the target in a calcium dependent manner. Subsequent polymerization with other perforin molecules forms a pore in the membrane. These pores allow the free influx and efflux of ions and proteins though the cell membrane. This disruption to homeostasis results in tonic shock which can ultimately lead to apoptosis and DNA degradation (Osinska et al. 2014).

Genetic abnormalities to the perforin gene have been shown to be linked to abnormal immune system functioning, the development of immune diseases as well as lymphomas and leukemias (Osinska et al. 2014).

This clone dG9 has been used in flow cytometry experiments to examine intracellular perforin in tumour specific CD8+ T cells incubated with autologous primary breast cancer cells and Tregs (Su et al. 2017).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
TRIS buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin
Immunogen
Purified granules from human YT lymphoma cell line
Purity
>95% by SDS PAGE
Purity
>95% by SDS PAGE
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
Storage
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P14222
Entrez Gene
PRF1
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0005509 calcium ion binding
GO:0002357 defense response to tumor cell
GO:0002418 immune response to tumor cell
GO:0006915 apoptosis
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0006968 cellular defense response
GO:0019835 cytolysis
GO:0022829 wide pore channel activity
GO:0031904 endosome lumen
GO:0051260 protein homooligomerization
GO:0044194 cytolytic granule
GO:0051607 defense response to virus
Regulatory
For research purposes only

Applications of Perforin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1
Immunocytochemistry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Flow Cytometry 1 Neat
  1. 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
  1. 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG2b Negative Control MCA691 F 100 Tests loader
Mouse IgG2b Negative Control:RPE MCA691PE F 100 Tests loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Leucoperm™ BUF09B F 250 Tests
Leucoperm™ BUF09B F 250 Tests