HLA A2 antibody | BB7.2

Mouse anti Human HLA A2

Product Type
Monoclonal Antibody
Clone
BB7.2
Isotype
IgG2b
Specificity
HLA A2

Product Code Applications Pack Size List Price Your Price Qty
MCA2090
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 0.2 mg loader
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loader
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Mouse anti Human HLA A2 antibody, clone BB7.2 recognizes the human HLA-A2 histocompatability antigen. The epitope recognized by this antibody has been studied extensively and would appear to include the carboxy-terminus of the alpha-2 helix and a turn on one of the underlying beta strands.

Mouse anti Human HLA A2 antibody, clone BB7.2 may be used for the flow cytometric detection of HLA-A2 expression and has also been used for immunoaffinity purification of HLA-A2 molecules. Functionally Mouse anti Human HLA A2 antibody, clone BB7.2 is reported to inhibit MHC restricted cellular cytotoxicity.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Papain solubilized HLA-A2
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 10ug/ml
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Histology Positive Control Tissue
Tonsil

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References for HLA A2 antibody

  1. Drukker, M. et al. (2006) Human embryonic stem cells and their differentiated derivatives are less susceptible to immune rejection than adult cells.
    Stem Cells. 24 (2): 221-9.
  2. Parham, P. & Brodsky, F.M. (1981) Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28.
    Hum Immunol. 3 (4): 277-99.
  3. Hogan, K.T. & Brown, S.L. (1992) Localization and characterization of serologic epitopes on HLA-A2.
    Hum Immunol. 33 (3): 185-92.
  4. Harig, S. et al. (2001) Induction of cytotoxic T-cell responses against immunoglobulin V region-derived peptides modified at human leukocyte antigen-A2 binding residues.
    Blood. 98 (10): 2999-3005.
  5. Pantenburg, B. et al. (2010) Human CD8(+) T cells clear Cryptosporidium parvum from infected intestinal epithelial cells.
    Am J Trop Med Hyg. 82:600-7.
  6. Duncan, L.M. et al. (2010) Stabilization of an E3 ligase-E2-ubiquitin complex increases cell surface MHC class I expression.
    J Immunol. 184: 6978-85.
  7. Wang, B. et al. (2004) Identification of an HLA-A*0201-restricted CD8+ T-cell epitope SSp-1 of SARS-CoV spike protein.
    Blood. 104: 200-6.
  8. Wooldridge, L. et al. (2007) Enhanced immunogenicity of CTL antigens through mutation of the CD8 binding MHC class I invariant region.
    Eur J Immunol. 37: 1323-33.
  9. View The Latest Product References
  10. Wooldridge, L. et al. (2010) MHC class I molecules with Superenhanced CD8 binding properties bypass the requirement for cognate TCR recognition and nonspecifically activate CTLs.
    J Immunol. 184: 3357-66.
  11. Popovic, J. et al. (2011) The only proposed T-cell epitope derived from the TEL-AML1 translocation is not naturally processed.
    Blood. 118 (4): 946-54.
  12. Schmidt, J. et al. (2011) Reversible Major Histocompatibility Complex I-Peptide Multimers Containing Ni2+-Nitrilotriacetic Acid Peptides and Histidine Tags Improve Analysis and Sorting of CD8+ T Cells.
    J Biol Chem. 286: 41723-35.
  13. Brooks, S.E. et al. (2015) Application of the pMHC Array to Characterise Tumour Antigen Specific T Cell Populations in Leukaemia Patients at Disease Diagnosis.
    PLoS One. 10 (10): e0140483.
  14. Rothe, K. et al. (2016) Latent Cytomegalovirus Infection in Rheumatoid Arthritis and Increased Frequencies of Cytolytic LIR-1+CD8+ T Cells.
    Arthritis Rheumatol. 68 (2): 337-46.
  15. De Angelis Rigotti, F. et al. (2017) MARCH9-mediated ubiquitination regulates MHC I export from the TGN.
    Immunol Cell Biol. 95 (9): 753-64.
  16. Kim, J.Y. et al. (2017) Detection of Donor-Derived Microparticles in the Peripheral Blood of a Hand Transplant Recipient During Rejection.
    Transplant Direct. 3 (3): e131.
  17. Dockree, T. et al. (2017) CD8+ T-cell specificity is compromised at a defined MHCI/CD8 affinity threshold.
    Immunol Cell Biol. 95 (1): 68-76.
  18. Sun, W. et al. (2018) A modified HLA-A*0201-restricted CTL epitope from human oncoprotein (hPEBP4) induces more efficient antitumor responses.
    Cell Mol Immunol. 15 (8): 768-81.
  19. Obenaus, M. et al. (2015) Identification of human T-cell receptors with optimal affinity to cancer antigens using antigen-negative humanized mice.
    Nat Biotechnol. 33 (4): 402-7.
  20. Walseng, E. et al. (2015) Soluble T-cell receptors produced in human cells for targeted delivery.
    PLoS One. 10 (4): e0119559.

Flow Cytometry

Functional Assays

RRID
AB_324652
UniProt
P01892
Entrez Gene
HLA-A
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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