HLA A2 antibody | BB7.2
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Mouse anti Human HLA A2
- Product Type
- Monoclonal Antibody
- HLA A2
|Mouse anti Human HLA A2 antibody, clone BB7.2 recognizes the human HLA-A2 histocompatability antigen. The epitope recognized by this antibody has been studied extensively and would appear to include the carboxy-terminus of the alpha-2 helix and a turn on one of the underlying beta strands.
Mouse anti Human HLA A2 antibody, clone BB7.2 may be used for the flow cytometric detection of HLA-A2 expression and has also been used for immunoaffinity purification of HLA-A2 molecules. Functionally Mouse anti Human HLA A2 antibody, clone BB7.2 is reported to inhibit MHC restricted cellular cytotoxicity.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Papain solubilized HLA-A2
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood
- Histology Positive Control Tissue
- Human tonsil
References for HLA A2 antibody
Parham, P. & Brodsky, F.M. (1981) Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28.
Hum Immunol. 3 (4): 277-99.
Hogan, K.T. & Brown, S.L. (1992) Localization and characterization of serologic epitopes on HLA-A2.
Hum Immunol. 33 (3): 185-92.
Drukker, M. et al. (2006) Human embryonic stem cells and their differentiated derivatives are less susceptible to immune rejection than adult cells.
Stem Cells. 24 (2): 221-9.
Pantenburg, B. et al. (2010) Human CD8(+) T cells clear Cryptosporidium parvum from infected intestinal epithelial cells.
Am J Trop Med Hyg. 82:600-7.
Duncan, L.M. et al. (2010) Stabilization of an E3 ligase-E2-ubiquitin complex increases cell surface MHC class I expression.
J Immunol. 184: 6978-85.
Wang, B. et al. (2004) Identification of an HLA-A*0201-restricted CD8+ T-cell epitope SSp-1 of SARS-CoV spike protein.
Blood. 104: 200-6.
Wooldridge, L. et al. (2007) Enhanced immunogenicity of CTL antigens through mutation of the CD8 binding MHC class I invariant region.
Eur J Immunol. 37: 1323-33.
Wooldridge, L. et al. (2010) MHC class I molecules with Superenhanced CD8 binding properties bypass the requirement for cognate TCR recognition and nonspecifically activate CTLs.
J Immunol. 184: 3357-66.
View The Latest Product References
Popovic, J. et al. (2011) The only proposed T-cell epitope derived from the TEL-AML1 translocation is not naturally processed.
Blood. 118 (4): 946-54.
Schmidt, J. et al. (2011) Reversible Major Histocompatibility Complex I-Peptide Multimers Containing Ni2+-Nitrilotriacetic Acid Peptides and Histidine Tags Improve Analysis and Sorting of CD8+ T Cells.
J Biol Chem. 286: 41723-35.
Brooks, S.E. et al. (2015) Application of the pMHC Array to Characterise Tumour Antigen Specific T Cell Populations in Leukaemia Patients at Disease Diagnosis.
PLoS One. 10 (10): e0140483.
Rothe, K. et al. (2016) Latent Cytomegalovirus Infection in Rheumatoid Arthritis and Increased Frequencies of Cytolytic LIR-1+CD8+ T Cells.
Arthritis Rheumatol. 68 (2): 337-46.
De Angelis Rigotti, F. et al. (2017) MARCH9-mediated ubiquitination regulates MHC I export from the TGN.
Immunol Cell Biol. 95 (9): 753-64.
Kim, J.Y. et al. (2017) Detection of Donor-Derived Microparticles in the Peripheral Blood of a Hand Transplant Recipient During Rejection.
Transplant Direct. 3 (3): e131.
Dockree, T. et al. (2017) CD8+ T-cell specificity is compromised at a defined MHCI/CD8 affinity threshold.
Immunol Cell Biol. 95 (1): 68-76.
Sun, W. et al. (2018) A modified HLA-A*0201-restricted CTL epitope from human oncoprotein (hPEBP4) induces more efficient antitumor responses.
Cell Mol Immunol. 15 (8): 768-81.
Obenaus, M. et al. (2015) Identification of human T-cell receptors with optimal affinity to cancer antigens using antigen-negative humanized mice.
Nat Biotechnol. 33 (4): 402-7.
Walseng, E. et al. (2015) Soluble T-cell receptors produced in human cells for targeted delivery.
PLoS One. 10 (4): e0119559.
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