Sandwich ELISA analysis of human granzyme B using Mouse anti Human granzyme B (MCA2120) as a capture reagent and biotinylated Mouse anti Human granzyme B (MCA2119B) as a detection reagent with recombinant human granzyme B as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Human plasma samples are diluted i:2 (green) and 1:4 (blue)
Mouse anti Human granzyme B antibody, clone GB10 recognizes the serine protease Granzyme B, which is expressed within the granules of cytolytic lymphocytes (CTLs).
Granzyme B plays a key role in the induction of apoptosis by CTLs. After delivery to the target cell Granzyme B activates the cascade of caspases that finally results in cell death.
N.B. Antibody reactivity and working conditions may vary between species.
Purified IgG - liquid
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
0.001% Thiomersal, 1% Bovine Serum Albumin
Purified human Granzyme B.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Reagents In The Kit
Preparing The Antibody
TRIS buffered saline.
Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Mouse anti Human granzyme B antibody, clone GB10 has been reported to function as a detection reagent in sandwich ELISA assays for soluble Granzyme B in conjunction with Mouse anti Human granzyme B antibody, clone GB11 (MCA2120) used as a capture reagent.