CD47 antibody | 1/1A4

Mouse anti Human CD47

Product Type
Monoclonal Antibody
Clone
1/1A4
Isotype
IgG2b
Specificity
CD47

Product Code Applications Pack Size List Price Your Price Qty
MCA2514GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 0.1 mg loader
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loader
MCA2514
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SDS Safety Datasheet SDS
C F IP 0.2 mg loader
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loader
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Mouse anti Human CD47 antibody, clone 1/1A4 recognizes human CD47, also known as integrin-associated protein, a 50kDa glycoprotein expressed on all haematopoietic cell lines, epithelial cells, endothelial cells, fibroblasts and many tumour cell lines.

CD47 is involved in several cellular processes including regulation of T cell and dendritic cell activation, regulation of neutrophil (PMN) chemotaxis, caspase-independent apoptosis, and may act as a signal transducer in the regulation of cation fluxes across cell membranes. CD47 has been identified as a ligand for the inhibitory macrophage receptor known as signal regulatory protein alpha (SIRPalpha) and as a receptor for thrombospondin-1.

Cross-linking of CD47 with clone 1/1A4 co-stimulates the proliferation of T cells (Waclavicek et al. 1993).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Human erythrocytes.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS-1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/100
Immunohistology - Frozen
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Histology Positive Control Tissue
Human tonsil

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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Mouse IgG2b Negative Control

References for CD47 antibody

  1. Waclavicek, M. et al. (1997) T cell stimulation via CD47: agonistic and antagonistic effects of CD47 monoclonal antibody 1/1A4.
    J Immunol. 159 (11): 5345-54.

Further Reading

  1. Frazier, W.A. et al. (1999) The thrombospondin receptor integrin-associated protein (CD47) functionally couples to heterotrimeric Gi.
    J Biol Chem. 274 (13): 8554-60.

Synonyms
SIRP LIGAND
RRID
AB_2074826
UniProt
Q08722
Entrez Gene
CD47
GO Terms
GO:0005515 protein binding
GO:0007596 blood coagulation
GO:0007155 cell adhesion
GO:0005887 integral to plasma membrane
GO:0007229 integrin-mediated signaling pathway
GO:0008284 positive regulation of cell proliferation
GO:0022409 positive regulation of cell-cell adhesion
GO:0034329 cell junction assembly
GO:0050870 positive regulation of T cell activation
GO:0050900 leukocyte migration
GO:0070053 thrombospondin receptor activity
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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