CD235a antibody | YTH89.1
Rat anti Human CD235a:StarBright Blue 700
- Product Type
- Monoclonal Antibody
- Clone
- YTH89.1
- Isotype
- IgG2b
- Specificity
- CD235a
Antibody Quality and Performance Guarantee
Data acquired on the ZE5 Cell analyser.
Figure B. Alexa Fluor®488 conjugated Mouse anti Human CD47 antibody, clone 1/1A4 (MCA2514A488) and StarBright Blue 700 conjugated Rat anti Human CD235a (MCA506SBB700).
All experiments performed on human blood gated on erythrocytes. Data acquired on the ZE5 Cell Analyzer.
Figure B. Alexa Flour® 647 conjugated Mouse anti Human CD47 antibody, clone 1/1A4 (MCA2514A647) and StarBright Violet 570 conjugated Rat anti Human CD235a antibody, clone YTH89.1 (MCA506SBV570).
All experiments performed on human blood gated on erythrocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell analyser.
Figure B. Alexa Flour®647 conjugated Mouse anti Human CD47 anitbody, clone 1/1A4 (MCA2514A647) and StarBright Violet 570 conjugated Rat anti Human CD235a antibody, clone YTH89.1 (MCA506SBV570).
All experiments performed on human blood gated on erythrocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell analyser.
Figure B. Alexa Fluor® 647 conjugated Mouse anti Human CD47 antibody, clone 1/1A4 (MCA2514A647) and StarBright Violet 440 conjugated Rat anti Human CD235a antibody, clone YTH89.1 (MCA506SBV440). All experiments performed on human blood gated on erythrocytes.
Data acquired on the ZE5 Cell analyser.
Figure B. Alexa Flour® 647 conjugated Mouse anti Human CD47 antibody, clone 1/1A4 (MCA2514A647) and StarBright Violet 610 conjugated Rat anti Human CD235a antibody, clone YTH89.1 (MCA506SBV610). All experiments performed on human blood gated on erythrocytes, in the presence of 10% human serum.
Data acquired on the ZE5 Cell analyser.
Filter by Application:
P F Reset| Rat anti Human CD235a antibody, clone YTH89.1 recognizes glycophorin A, a major sialoglycoprotein of the human erythrocyte membrane. |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to StarBright Blue 700 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20 - Approx. Protein Concentrations
- For information on the concentration of our StarBright Dye conjugated reagents please visit our FAQ page.
- Fusion Partners
- Spleen cells from immunized DA rats were fused with cells of the Y3/Ag.1.2.3.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) StarBright Blue 700 473 703 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
This product is shipped at ambient temperature.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 |  |
Neat |
- 1Staining at lower temperatures generally requires longer incubation to reach antibody binding equilibrium, and this effect is more pronounced for StarBright Dyes due to their larger size. For optimum staining, Bio-Rad recommends incubating samples either overnight at 4°C or 1 hour at room temperature.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 5μl of the suggested working dilution to label 0.5x106 cells in 100μl. Best practices suggest a 5 min centrifugation at 6,000g prior to sample application.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Human Seroblock | ||||||
| Human Seroblock | BUF070B | F | 200 Test | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Human Seroblock | ||||||
References for CD235a antibody
-
Outram, S. et al. (1988) Erythromyeloid lineage fidelity is conserved in erythroleukaemia.
Leuk Res. 12 (8): 651-7. -
Jokiranta, T.S. & Meri, S. (1993) Biotinylation of monoclonal antibodies prevents their ability to activate the classical pathway of complement.
J Immunol. 151 (4): 2124-31. -
Hoang, T. et al. (1996) Opposing effects of the basic helix-loop-helix transcription factor SCL on erythroid and monocytic differentiation.
Blood. 87: 102-11. -
Babiker, A.A. et al. (2002) Transfer of prostasomal CD59 to CD59-deficient red blood cells results in protection against complement-mediated hemolysis.
Am J Reprod Immunol. 47 (3): 183-92. -
Lahlil, R. et al. (2004) SCL assembles a multifactorial complex that determines glycophorin A expression.
Mol Cell Biol. 24: 1439-52. -
Challier, J.C. et al. (2005) Immunocytological evidence for hematopoiesis in the early human placenta.
Placenta. 26: 282-8. -
Huang, Y.C. et al. (2009) Oral small-molecule tyrosine kinase inhibitor midostaurin (PKC412) inhibits growth and induces megakaryocytic differentiation in human leukemia cells.
Toxicol In Vitro. 23: 979-85. -
Tiziani, S. et al. (2009) Metabolomic profiling of drug responses in acute myeloid leukaemia cell lines.
PLoS One. 2009;4(1):e4251.
View The Latest Product References
-
Basu, S.
(2010) Erythrocyte membrane defects and asymmetry in paroxysmal nocturnal hemoglobinuria and myelodysplastic syndrome.
Hematology. 15: 236-9. -
Saha, S. et al. (2011) Elevated levels of redox regulators, membrane-bound globin chains, and cytoskeletal protein fragments in hereditary spherocytosis erythrocyte proteome.
Eur J Haematol. 87: 259-66. -
Lucky AB et al. (2016) Plasmodium knowlesi Skeleton-Binding Protein 1 Localizes to the 'Sinton and Mulligan' Stipplings in the Cytoplasm of Monkey and Human Erythrocytes.
PLoS One. 11 (10): e0164272. -
Scanlon, V.M. et al. (2022) Multiparameter analysis of timelapse imaging reveals kinetics of megakaryocytic erythroid progenitor clonal expansion and differentiation.
Sci Rep. 12 (1): 16218.
- Synonyms
- Glycophorin A
- UniProt
- P02724
- Entrez Gene
- GYPA
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0005624 membrane fraction
- GO:0042802 identical protein binding
- GO:0044419 interspecies interaction between organisms
MCA506SBB700
100008104
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