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CD85j antibody | 4F9

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Mouse anti Human CD85j:StarBright UltraViolet 700

Product Type
Monoclonal Antibody
Clone
4F9
Isotype
IgG1
Specificity
CD85j

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Product Code Applications Pack Size List Price Your Price Qty
MCA2515SBUV700
Datasheet Datasheet
SDS Safety Datasheet SDS
F * 100 Tests/0.5ml loader
List Price Your Price
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Anti Human CD85j Antibody, clone 4F9 thumbnail image 1 Anti Human CD85j Antibody, clone 4F9 thumbnail image 2 Anti Human CD85j Antibody, clone 4F9 thumbnail image 3 Anti Human CD85j Antibody, clone 4F9 thumbnail image 4 Anti Human CD85j Antibody, clone 4F9 thumbnail image 5
Anti Human CD85j Antibody, clone 4F9 gallery image 1
Figure A. StarBright Blue 580 conjugated Mouse anti Human CD19 (MCA1940SBB580) and Alexa Fluor 647 conjugated Mouse IgG1 isotype control (MCA928A647). Figure B. StarBright Blue 580 conjugated Mouse anti Human CD19 (MCA1940SBB580) and Alexa Fluor 647 conjugated Mouse anti Human CD85j (MCA2515A647). All experiments performed on red cell lysed human blood gated on single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD85j Antibody, clone 4F9 gallery image 2
Human whole blood was stained with Anti-Human CD19 StarBright Blue 810 (MCA1940SBB810) only (Figure A) or with Anti-Human CD19 StarBright Blue 810 (MCA1940SBB810) and Anti-Human CD85j StarBright UltraViolet 540 (MCA2515SBUV540) (Figure B). Experiments were performed on isolated red-cell lysed blood, gated on live, single mononuclear cells. Data were acquired on a Cytek Aurora.
Anti Human CD85j Antibody, clone 4F9 gallery image 3
Human whole blood was stained with Anti-Human CD19 StarBright Blue 810 (MCA1940SBB810) only (Figure A) or with Anti-Human CD19 StarBright Blue 810 (MCA1940SBB810) and Anti-Human CD85j StarBright UltraViolet 700 (MCA2515SBUV700) (Figure B). Experiments were performed on isolated red-cell lysed blood, gated on live, single mononuclear cells. Data were acquired on a Cytek Aurora.
Anti Human CD85j Antibody, clone 4F9 gallery image 4
Human whole blood was stained with Anti-Human CD19 StarBright Blue 615 (MCA1940SBB615) only (Figure A) or with Anti-Human CD19 StarBright Blue 615 (MCA1940SBB615) and Anti-Human CD85j StarBright UltraViolet 770 (MCA2515SBUV770) (Figure B). Experiments were performed on isolated red-cell lysed blood, gated on live, single mononuclear cells. Data were acquired on a Cytek Aurora.
Anti Human CD85j Antibody, clone 4F9 gallery image 5
Human whole blood was stained with Anti-Human CD19 StarBright Blue 810 (MCA1940SBB810) only (Figure A) or with Anti-Human CD19 StarBright Blue 810 (MCA1940SBB810) and Anti-Human CD85j StarBright Violet 535 (MCA2515SBV535) (Figure B). Experiments were performed on isolated red-cell lysed blood, gated on live, single mononuclear cells. Data were acquired on a Cytek Aurora.

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Mouse anti Human CD85j antibody, clone 4F9 recognizes CD85j, a member of the leukocyte immunoglobulin-like receptor (LIR) family.

CD85j is a receptor for MHC Class I molecules and ligand binding results in inhibitory signals and down-regulation of the immune response.

CD85j is expressed predominantly on B-cells and monocytes, and at lower levels on dendritic cells, T-cells and natural killer (NK) cells.

Target Species
Human
Product Form
Purified IgG conjugated to StarBright UltraViolet 700 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Immunogen
Monocyte derived dendritic cells.
Approx. Protein Concentrations
For information on the concentration of our StarBright Dye conjugated reagents please visit our FAQ page.
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse X63-Ag8.653 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
StarBright UltraViolet 700 340 700
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 Neat
  1. 1Staining at lower temperatures generally requires longer incubation to reach antibody binding equilibrium, and this effect is more pronounced for StarBright Dyes due to their larger size. For optimum staining, Bio-Rad recommends incubating samples either overnight at 4°C or 1 hour at room temperature.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5μl of the suggested working dilution to label 0.5x106 cells in 100μl. Best practices suggest a 5 min centrifugation at 6,000g prior to sample application.

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Further Reading

  1. Colonna, M. et al. (1999) A novel family of Ig-like receptors for HLA class I molecules that modulate function of lymphoid and myeloid cells.
    J Leukoc Biol. 66 (3): 375-81.
  2. Borges, L. et al. (1997) A family of human lymphoid and myeloid Ig-like receptors, some of which bind to MHC class I molecules.
    J Immunol. 159 (11): 5192-6.

Synonyms
LILRB1
UniProt
Q8NHL6
Entrez Gene
LILRB1
GO Terms
GO:0005886 plasma membrane
GO:0009615 response to virus
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0008157 protein phosphatase 1 binding
GO:0050776 regulation of immune response

MCA2515SBUV700

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