CD25 antibody | P4A10
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F ResetMouse anti Dog CD25
- Product Type
- Monoclonal Antibody
- Clone
- P4A10
- Isotype
- IgG1
- Specificity
- CD25
Antibody Quality and Performance Guarantee
| Mouse anti Dog CD25, clone P4A10 recognizes the canine homologue of the human CD25 cell surface antigen, also known at IL-2Rα, a glycoprotein of approximately 55 kDa expressed primarily by activated T lymphocytes (Abrams et al. 2010). The IL-2 receptor is composed of 3 subunits, an α chain (CD25), a β chain (CD122) and a γ chain (CD132), CD25 functions as a low affinity receptor for IL-2. Antibodies to CD4, Mouse anti Human CD25 antibody, clone ACT1, which has demonstrated cross reactivity to canine CD25 (Rissetto et al. 2010). However this clone has poor affinity for canine CD25. The development of clone P4A10 offers a specific monoclonal Mouse anti Canine CD25, demonstrating a greater affinity for canine CD25 than the cross reactive anti human antibody (Abrams et al. 2010). The dog is an important veterinary species in its own right. In addition dogs are used as an animal model in the study of a number of serious human disease states including various forms cancers (Paoloni et al. 2008), and in genetically related diseases of the hemopoietic system (Bauer Jr.et al. 2009). |
- Target Species
- Dog
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Carrier Free
- Yes
- Immunogen
- Enriched and stimulated canine T cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized RBF/DnJ mice were fused with cells of the mouse P3-653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Flow Cytometry |  |
1/50 | 1/100 |
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin | |||
| Immunohistology - Resin | |||
| Immunoprecipitation | |||
| Western Blotting 1 | |
- 1Clone P4A10 produces a double band at approximately 55kDa under non-reducing conditions. A non-specific band may be observed at 116kDa
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Western Blotting
- Clone P4A10 detects a double band of approximately 55kDa in PMA-IO activated canine PBMC lysates.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| Description | Mouse IgG1 Negative Control | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat anti Dog CD4 | MCA1038GA | C F | 0.1 mg |
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| Description | Rat anti Dog CD4 | ||||||
| Rat anti Dog CD8 | MCA1039GA | C F IF | 0.1 mg |
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| Mouse anti Dog CD3:FITC | MCA1774F | F | 0.1 mg |
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| Description | Mouse anti Dog CD3:FITC | ||||||
| Mouse anti Dog CD3 | MCA1774GA | C F IF IP | 0.1 mg |
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| Description | Mouse anti Dog CD3 | ||||||
References for CD25 antibody
-
Abrams, V.K. et al. (2010) A novel monoclonal antibody specific for canine CD25 (P4A10): selection and evaluation of canine Tregs.
Vet Immunol Immunopathol. 135 (3-4): 257-65. -
Finn, J.D. et al. (2010) Eradication of neutralizing antibodies to factor VIII in canine hemophilia A after liver gene therapy.
Blood.116: 5842-8. -
Mizutani, N. et al. (2020) Measurement of the concentration of serum soluble interleukin-2 receptor alpha chain in dogs with lymphoma.
Vet Immunol Immunopathol. 225: 110054. -
Wesolowski, M. et al. (2023) Long-term changes of Th17 and regulatory T cells in peripheral blood of dogs with spinal cord injury after intervertebral disc herniation.
BMC Vet Res. 19 (1): 90.
Further Reading
-
Paoloni, M. & Khanna, C. (2008) Translation of new cancer treatments from pet dogs to humans.
Nat Rev Cancer. 8 (2): 147-56. -
Bauer, T.R. Jr et al. (2009) Potential large animal models for gene therapy of human genetic diseases of immune and blood cell systems.
ILAR J. 50 (2): 168-86. -
Rissetto, K.C. et al. (2010) Cloning and expression of canine CD25 for validation of an anti-human CD25 antibody to compare T regulatory lymphocytes in healthy dogs and dogs with osteosarcoma.
Vet Immunol Immunopathol. 135 (1-2): 137-45. -
Pinheiro, D. et al. (2011) Phenotypic and functional characterization of a CD4(+) CD25(high) FOXP3(high) regulatory T-cell population in the dog.
Immunology. 132 (1): 111-22.
- Synonyms
- IL-2R Alpha Chain
- UniProt
- O62802
- Entrez Gene
- IL2RA
- GO Terms
- GO:0016021 integral to membrane
MCA5916GA
1015
165730
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