CD3 antibody | CA17.2A12
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F IF ResetMouse anti Dog CD3
- Product Type
- Monoclonal Antibody
- Clone
- CA17.2A12
- Isotype
- IgG1
- Specificity
- CD3
Mouse anti Dog CD3 antibody, clone CA17.2A12 recognizes the canine CD3 cell surface antigen, expressed by thymocytes and mature T lymphocytes. CD3 is engaged in the surface expression of the T-cell antigen receptor (TCR) and the signal transduction pathway resulting from MHC ligand binding to the TCR. CD3 is made up of a number of invariant subchains of the immunoglobulin superfamily. Mouse anti Dog CD3 clone CA17.2A12 is a valuable flow cytometric and immunohistologic tool for canine lymphoma detection of T-cell origin (Miniscalco et al. 2003). |
- Target Species
- Dog
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Immunogen
- Affinity enriched TCR/CD3 membrane proteins isolated from thymocytes and the T cell line CLGL-90
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 1/25 | 1/50 | |
Immunofluorescence | |||
Immunohistology - Frozen | |||
Immunoprecipitation |
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgG1 Negative Control | MCA928 | F | 100 Tests | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Mouse IgG1 Negative Control |
References for CD3 antibody
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Moore, P.F. and Rossitto, P.V. (1993) Development of monoclonal antibodies to canine T cell receptor complex (TCR/CD3) and their utilisation in the diagnosis of T cell neoplasia.
Vet. Pathol. 30: 457. Abstract 117. -
Vernau, W and Moore, P. F. (1999) An immunophenotypic study of canine leukemias and preliminary assessment of clonality by polymerase chain reaction.
Vet Immunol Immunopathol. 69:145-64. -
Moreno, J. et al (1999) The immune response and PBMC subsets in canine visceral leishmaniasis before, and after, chemotherapy.
Vet Immunol Immunopathol. 71:181-95. -
McDonough, S. P. and Moore, P. F. (2000) Clinical, hematologic, and immunophenotypic characterization of canine large granular lymphocytosis.
Vet Pathol. 37:637-46. -
Byrne, K. et al (2000) A standardized gating technique for the generation of flow cytometry data for normal canine and normal feline blood lymphocytes.
Vet Immunol Immunopathol. 73:167-82. -
Out, T.A. et al. (2002) Local T-cell activation after segmental allergen challenge in the lungs of allergic dogs.
Immunology. 105: 499-508. -
Zentek, J. et al. (2002) Morphology and immunopathology of the small and large intestine in dogs with nonspecific dietary sensitivity.
J Nutr. 132: 1652S-4S. -
Hsiao, Y.W. et al (2004) Tumor-infiltrating lymphocyte secretion of IL-6 antagonizes tumor-derived TGF-beta 1 and restores the lymphokine-activated killing activity.
J Immunol. 172: 1508-14.
View The Latest Product References
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Watabe, A. et al. (2011) Alterations of lymphocyte subpopulations in healthy dogs with aging and in dogs with cancer.
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Duz, A.L. et al. (2014) The TcI and TcII Trypanosoma cruzi experimental infections induce distinct immune responses and cardiac fibrosis in dogs.
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Constantinoiu, C.C. et al. (2015) Mucosal tolerance of the hookworm Ancylostoma caninum in the gut of naturally infected wild dogs.
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Grøndahl-Rosado C et al. (2015) NCR1+ cells in dogs show phenotypic characteristics of natural killer cells.
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Miller, J. et al. (2015) Humoral and Cellular Immune Response in Canine Hypothyroidism.
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Schaut, R.G. et al. (2016) Recovery of antigen-specific T cell responses from dogs infected with Leishmania (L.) infantum by use of vaccine associated TLR-agonist adjuvant.
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Riondato, F. et al. (2016) Analytical and diagnostic validation of a flow cytometric strategy to quantify blood and marrow infiltration in dogs with large B-cell lymphoma.
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Bonnefont-Rebeix, C. et al. (2016) Characterization of a novel canine T-cell line established from a spontaneously occurring aggressive T-cell lymphoma with large granular cell morphology.
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Schaut, R.G. et al. (2016) Regulatory IgDhi B Cells Suppress T Cell Function via IL-10 and PD-L1 during Progressive Visceral Leishmaniasis.
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McGill, J.L. et al. (2016) Vaccination with an Attenuated Mutant of Ehrlichia chaffeensis Induces Pathogen-Specific CD4+ T Cell Immunity and Protection from Tick-Transmitted Wild-Type Challenge in the Canine Host.
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Martins, G.C. et al. (2018) Clinical-pathological and immunological biomarkers in dogs with atopic dermatitis.
Vet Immunol Immunopathol. 205: 58-64. -
Schmidli, M.R. et al. (2018) Inflammatory pattern of the infrapatellar fat pad in dogs with canine cruciate ligament disease.
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Akiyama, S. et al. (2019) Th17 cells increase during maturation in peripheral blood of healthy dogs.
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Aguiar-Soares, R.D.O. et al. (2020) Phase I and II Clinical Trial Comparing the LBSap, Leishmune(®), and Leish-Tec(®) Vaccines against Canine Visceral Leishmaniasis.
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Yang, Y. et al. (2021) Canine Multicentric Large B Cell Lymphoma with Increased Mott Cells Diagnosed by Flow Cytometry
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Konno, H. et al. (2022) An experimental challenge model for Leishmania donovani in beagle dogs, showing a similar pattern of parasite burden in the peripheral blood and liver.
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Wesolowski, M. et al. (2023) Long-term changes of Th17 and regulatory T cells in peripheral blood of dogs with spinal cord injury after intervertebral disc herniation.
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Archer, T.M. et al. (2018) In vivo effects of aspirin and cyclosporine on regulatory T cells and T-cell cytokine production in healthy dogs.
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Lee, G.W. et al. (2021) Case Report: Long-Term Survival of a Dog With Chronic Lymphocytic Leukemia Treated With Chlorambucil, Prednisolone, and Imatinib.
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Sainz, Á. et al. (2021) Effect of chemically modified tetracycline-8 (CMT-8) on hematology, blood chemistry, cytokines and peripheral blood lymphocyte subsets of healthy dogs.
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Anthonyraj, S. et al. (2024) Chicory root powder included as a prebiotic in different cereal-based diets for dogs: Influences on gut health, metabolic and immunological status
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Sheng, R. et al. (2023) Prognostic significance of CD25 expression in dogs with a noninvasive diagnosis of B-cell lymphoma treated with CHOP chemotherapy.
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Miguelena Chamorro, B. et al. (2023) Characterization of Canine Peyer's Patches by Multidimensional Analysis: Insights from Immunofluorescence, Flow Cytometry, and Single-Cell RNA Sequencing.
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Santos, M.F. et al. (2020) Immunophenotyping of Peripheral Blood, Lymph Node, and Bone Marrow T Lymphocytes During Canine Leishmaniosis and the Impact of Antileishmanial Chemotherapy.
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- RRID
- AB_1125250
- UniProt
- P27597
- Entrez Gene
- CD3E
- GO Terms
- GO:0016021 integral to membrane
- GO:0004888 transmembrane receptor activity
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