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CD3 antibody | CA17.2A12

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Anti Dog CD3 Antibody, clone CA17.2A12 thumbnail image 1 Anti Dog CD3 Antibody, clone CA17.2A12 thumbnail image 2 Anti Dog CD3 Antibody, clone CA17.2A12 thumbnail image 3 Anti Dog CD3 Antibody, clone CA17.2A12 thumbnail image 4 Anti Dog CD3 Antibody, clone CA17.2A12 thumbnail image 5 Anti Dog CD3 Antibody, clone CA17.2A12 thumbnail image 6 Anti Dog CD3 Antibody, clone CA17.2A12 thumbnail image 7 Anti Dog CD3 Antibody, clone CA17.2A12 thumbnail image 8 Anti Dog CD3 Antibody, clone CA17.2A12 thumbnail image 9 Anti Dog CD3 Antibody, clone CA17.2A12 thumbnail image 10
Anti Dog CD3 Antibody, clone CA17.2A12 gallery image 1
Figure A. APC conjugated Rat anti Canine CD4 (MCA1038APC) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. APC conjugated Rat anti Canine CD4 (MCA1038APC) and FITC conjugated Mouse anti Canine CD3 (MCA1774F). All experiments performed on red cell lysed canine blood gated on lymphocytes in the presence of 10% canine serum. Data acquired on the ZE5 Cell Analyzer.
Anti Dog CD3 Antibody, clone CA17.2A12 gallery image 2
Figure A. RPE conjugated Rat anti Dog CD4 (MCA1038PE) and Mouse IgG1 isotype control (MCA928) labeled with Goat anti Mouse FITC (STAR117F). Figure B. RPE conjugated Rat anti Dog CD4 (MCA1038PE) and Mouse anti Dog CD3 (MCA1774GA) labeled with Goat anti Mouse Dylight FITC (STAR117F). All experiments performed on red cell lysed canine blood gated lymphoid cells in the presence of 10% dog serum. Data acquired on the ZE5 Cell Analyzer.
Anti Dog CD3 Antibody, clone CA17.2A12 gallery image 3
Figure A. Alexa Fluor 700 conjugated Rat anti Canine CD8 (MCA1039A700) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. Alexa Fluor 700 conjugated Rat anti Canine CD8 (MCA1039A700) and FITC conjugated Mouse anti Canine CD3 (MCA1774F). All experiments performed on red cell lysed canine blood gated on lymphocytes in the presence of 10% canine serum. Data acquired on the ZE5 Cell Analyzer
Anti Dog CD3 Antibody, clone CA17.2A12 gallery image 4
Figure A. FITC conjugated Mouse anti Canine CD3 (MCA1774F) and RPE conjugated Rat IgG2a isotype control (MCA1212PE). Figure B. FITC conjugated Mouse anti Canine CD3 (MCA1774F) and RPE conjugated Rat anti Canine CD4 (MCA1038PE). All experiments performed on red cell lysed canine blood gated on mononuclear cells.
Anti Dog CD3 Antibody, clone CA17.2A12 gallery image 5
Figure A. RPE conjugated Rat anti Canine CD4 (MCA1038PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated Rat anti Canine CD4 (MCA1038PE) and FITC conjugated Mouse anti Canine CD3 (MCA1774F). All experiments performed on red cell lysed canine blood gated on mononuclear cells.
Anti Dog CD3 Antibody, clone CA17.2A12 gallery image 6
Figure A. RPE conjugated Rat anti Canine CD8 (MCA1039PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated Rat anti Canine CD8 (MCA1039PE) and FITC conjugated Mouse anti Canine CD3 (MCA1774F). All experiments performed on red cell lysed canine blood gated on mononuclear cells.
Anti Dog CD3 Antibody, clone CA17.2A12 gallery image 7
Figure A. FITC conjugated Mouse anti Canine CD3 (MCA1774F) and RPE conjugated Rat IgG1 isotype control (MCA6004PE). Figure B. FITC conjugated Mouse anti Canine CD3 (MCA1774F) and RPE conjugated Rat anti Canine CD8 (MCA1039PE). All experiments performed on red cell lysed canine blood gated on mononuclear cells.
Anti Dog CD3 Antibody, clone CA17.2A12 gallery image 8
Figure A. FITC conjugated Mouse anti Canine CD3 (MCA1774F) and purified Rat IgG2a isotype control (MCA6005) detected with Goat anti Rat IgG2a DyLight 649. Figure B. FITC conjugated Mouse anti Canine CD3 (MCA1774F) and purified Mouse anti Canine CD90 (MCA1036GA) detected with Goat anti Rat IgG2a DyLight 649. All experiments performed on red cell lysed canine blood gated on mononuclear cells.
Anti Dog CD3 Antibody, clone CA17.2A12 gallery image 9
Figure A. Purified Rat anti Canine CD90 (MCA1036GA) detected with Goat anti Rat IgG2a DyLight 649 and Mouse IgG1 FITC isotype control (MCA928F). Figure B. Purified Rat anti Canine CD90 (MCA1036GA) detected with Goat anti Rat IgG2a PE and Mouse anti Canine CD3 FITC (MCA1774F). All experiments performed on red cell lysed canine blood gated on mononuclear cells.
Anti Dog CD3 Antibody, clone CA17.2A12 gallery image 10
Published customer image:
Canine T cells labeled with FITC conjugated Mouse anti Canine CD3 antibody, clone CA17.2A12 (MCA1774F) by flow cytometry.
Image caption:
CD11b+CD14−MHCII− cells suppress T cell proliferation. Facs sorted CD11b+CD14−MHCII− cells isolated from a dog with osteosarcoma or healthy PBMCs were co-incubated with mitogen-stimulated CD4+ and CD8+ T cells isolated from a healthy dog for 72 hs. No stimulated cells were used as negative control. Proliferative responses were measured by 3H-thymidine incorporation from experiments performed in triplicate. CPM, counts per minute. Mean ± SEM are shown.

From: Goulart MR, Pluhar GE, Ohlfest JR (2012)
Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer.
PLoS ONE 7(3): e33274.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

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Mouse anti Dog CD3:FITC

Product Type
Monoclonal Antibody
Clone
CA17.2A12
Isotype
IgG1
Specificity
CD3

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Product Code Applications Pack Size List Price Your Price Qty
MCA1774F
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F 0.1 mg loader
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Mouse anti Dog CD3 antibody, clone CA17.2A12 recognizes the canine CD3 cell surface antigen, expressed by thymocytes and mature T lymphocytes. CD3 is engaged in the surface expression of the T-cell antigen receptor (TCR) and the signal transduction pathway resulting from MHC ligand binding to the TCR. CD3 is made up of a number of invariant subchains of the immunoglobulin superfamily.

Mouse anti Dog CD3 clone CA17.2A12 is a valuable flow cytometric and immunohistologic tool for canine lymphoma detection of T-cell origin (Miniscalco et al. 2003).

Target Species
Dog
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Affinity enriched TCR/CD3 membrane proteins isolated from thymocytes and the T cell line CLGL-90
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.

N.B.. MCA1774F should not be used with MCA1781PE (mouse anti canine B-cells), in dual colour flow cytometry, due to non-specific interactions between the two reagents.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
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Description Mouse IgG1 Negative Control:FITC

References for CD3 antibody

  1. Machado, G.F. et al. (2011) Intravascular Lymphomatosis in the Central Nervous System of Dogs: Immunohistochemical Investigation in Two Cases
    Braz J Vet Pathol 4: 47-51.
  2. Moore, P.F. and Rossitto, P.V. (1993) Development of monoclonal antibodies to canine T cell receptor complex (TCR/CD3) and their utilisation in the diagnosis of T cell neoplasia.
    Vet. Pathol. 30: 457. Abstract 117.
  3. McDonough, S. P. and Moore, P. F. (2000) Clinical, hematologic, and immunophenotypic characterization of canine large granular lymphocytosis.
    Vet Pathol. 37:637-46.
  4. Moore, P.F. et al. (2006) Canine hemophagocytic histiocytic sarcoma: a proliferative disorder of CD11d+ macrophages.
    Vet Pathol. 43 (5): 632-45.
  5. Vernau, W and Moore, P. F. (1999) An immunophenotypic study of canine leukemias and preliminary assessment of clonality by polymerase chain reaction.
    Vet Immunol Immunopathol. 69:145-64.
  6. Moreno, J. et al (1999) The immune response and PBMC subsets in canine visceral leishmaniasis before, and after, chemotherapy.
    Vet Immunol Immunopathol. 71:181-95.
  7. Fellman, C.L. et al. (2011) Cyclosporine A affects the in vitro expression of T cell activation-related molecules and cytokines in dogs.
    Vet Immunol Immunopathol. 140: 175-80.
  8. Watabe, A. et al. (2011) Alterations of lymphocyte subpopulations in healthy dogs with aging and in dogs with cancer.
    Vet Immunol Immunopathol. 142: 189-200.
    9. View The Latest Product References
  9. Hsiao, Y.W. et al (2004) Tumor-infiltrating lymphocyte secretion of IL-6 antagonizes tumor-derived TGF-beta 1 and restores the lymphokine-activated killing activity.
    J Immunol. 172: 1508-14.
  10. Huang, Y.C. et al. (2008) CD5-low expression lymphocytes in canine peripheral blood show characteristics of natural killer cells.
    J Leukoc Biol. 84: 1501-10.
  11. Out, T.A. et al. (2002) Local T-cell activation after segmental allergen challenge in the lungs of allergic dogs.
    Immunology. 105: 499-508.
  12. Zentek, J. et al. (2002) Morphology and immunopathology of the small and large intestine in dogs with nonspecific dietary sensitivity.
    J Nutr. 132: 1652S-4S.
  13. Hai, M. et al. (2008) Potential genotoxicity from integration sites in CLAD dogs treated successfully with gammaretroviral vector-mediated gene therapy.
    Gene Ther. 15: 1067-71.
  14. Altmann, S. et al. (2008) High Mobility Group Box 1-Protein expression in canine haematopoietic cells and influence on canine peripheral blood mononuclear cell proliferative activity
    Vet Immunol Immunopathol. 126: 367-72.
  15. Ting-De Ravin, S.S. et al. (2006) Correction of canine X-linked severe combined immunodeficiency by in vivo retroviral gene therapy.
    Blood. 107: 3091-7.
  16. Miranda, S. et al. (2007) Characterization of circulating lymphocyte subpopulations in canine leishmaniasis throughout treatment with antimonials and allopurinol.
    Vet Parasitol. 144: 251-60.
  17. Maiolini, A. et al. (2012) Toll-like receptors 4 and 9 are responsible for the maintenance of the inflammatory reaction in canine steroid-responsive meningitis-arteritis, a large animal model for neutrophilic meningitis.
    J Neuroinflammation. 9: 226.
  18. Aresu, L. et al. (2014) VEGF and MMP-9: biomarkers for canine lymphoma.
    Vet Comp Oncol. 12: 29-36.
  19. Schaut, R.G. et al. (2016) Regulatory IgDhi B Cells Suppress T Cell Function via IL-10 and PD-L1 during Progressive Visceral Leishmaniasis.
    J Immunol. 196 (10): 4100-9.
  20. Villaescusa A et al. (2012) Evaluation of peripheral blood lymphocyte subsets in family-owned dogs naturally infected by Ehrlichia canis.
    Comp Immunol Microbiol Infect Dis. 35 (4): 391-6.
  21. Riondato, F. et al. (2016) Analytical and diagnostic validation of a flow cytometric strategy to quantify blood and marrow infiltration in dogs with large B-cell lymphoma.
    Cytometry B Clin Cytom. 90 (6): 525-530.
  22. Byrne, K. et al (2000) A standardized gating technique for the generation of flow cytometry data for normal canine and normal feline blood lymphocytes.
    Vet Immunol Immunopathol. 73:167-82.
  23. Perosso, J. et al. (2014) Alteration of sFAS and sFAS ligand expression during canine visceral leishmaniosis.
    Vet Parasitol. 205 (3-4): 417-23.
  24. Grøndahl-Rosado C et al. (2015) NCR1+ cells in dogs show phenotypic characteristics of natural killer cells.
    Vet Res Commun. 39 (1): 19-30.
  25. Miller, J. et al. (2015) Humoral and Cellular Immune Response in Canine Hypothyroidism.
    J Comp Pathol. 153 (1): 28-37.
  26. McGill, J.L. et al. (2016) Vaccination with an Attenuated Mutant of Ehrlichia chaffeensis Induces Pathogen-Specific CD4+ T Cell Immunity and Protection from Tick-Transmitted Wild-Type Challenge in the Canine Host.
    PLoS One. 11 (2): e0148229.
  27. Constantinoiu CC et al. (2015) Mucosal tolerance of the hookworm Ancylostoma caninum in the gut of naturally infected wild dogs.
    Parasite Immunol. Jul 27 [Epub ahead of print]
  28. Duz, A.L. et al. (2014) The TcI and TcII Trypanosoma cruzi experimental infections induce distinct immune responses and cardiac fibrosis in dogs.
    Mem Inst Oswaldo Cruz. 109 (8): 1005-13.
  29. Mie, K. et al. (2016) Change in peripheral blood lymphocyte count in dogs following adoptive immunotherapy using lymphokine-activated T killer cells combined with palliative tumor resection.
    Vet Immunol Immunopathol. 177: 58-63.
  30. Schaut, R.G. et al. (2016) Recovery of antigen-specific T cell responses from dogs infected with Leishmania (L.) infantum by use of vaccine associated TLR-agonist adjuvant.
    Vaccine. 34 (44): 5225-34.
  31. Gelain, M.E. et al. (2014) CD44 in canine leukemia: analysis of mRNA and protein expression in peripheral blood.
    Vet Immunol Immunopathol. 159 (1-2): 91-6.
  32. Michael, H.T. et al. (2013) Isolation and characterization of canine natural killer cells.
    Vet Immunol Immunopathol. 155 (3): 211-7.
  33. Bonnefont-Rebeix, C. et al. (2016) Characterization of a novel canine T-cell line established from a spontaneously occurring aggressive T-cell lymphoma with large granular cell morphology.
    Immunobiology. 221 (1): 12-22.
  34. Schmidli, M.R. et al. (2018) Inflammatory pattern of the infrapatellar fat pad in dogs with canine cruciate ligament disease.
    BMC Vet Res. 14 (1): 161.
  35. Aricò, A. et al. (2013) The role of vascular endothelial growth factor and matrix metalloproteinases in canine lymphoma: in vivo and in vitro study.
    BMC Vet Res. 9: 94.
  36. Aguiar-Soares, R.D.O. et al. (2020) Phase I and II Clinical Trial Comparing the LBSap, Leishmune®, and Leish-Tec® Vaccines against Canine Visceral Leishmaniasis.
    Vaccines (Basel). 8 (4)Nov 17 [Epub ahead of print].
  37. Marchetti, C. et al. (2020) Profile of gamma-delta (γδ) T lymphocytes in the peripheral blood of crossbreed dogs during stages of life and implication in aging.
    BMC Vet Res. 16 (1): 278.
  38. Akiyama, S. et al. (2019) Th17 cells increase during maturation in peripheral blood of healthy dogs.
    Vet Immunol Immunopathol. 209: 17-21.
  39. Martins, G.C. et al. (2018) Clinical-pathological and immunological biomarkers in dogs with atopic dermatitis.
    Vet Immunol Immunopathol. 205: 58-64.
  40. Sayag, D. et al. (2020) Proof-of-concept study: Evaluation of plasma and urinary electrolytes as markers of response to L-asparaginase therapy in dogs with high-grade lymphoma.
    Vet Clin Pathol. 49 (3): 476-83.
  41. Lee, J. et al. (2021) Canine Natural Killer Cell-Derived Exosomes Exhibit Antitumor Activity in a Mouse Model of Canine Mammary Tumor.
    Biomed Res Int. 2021: 6690704.
  42. Rotolo, A. et al. (2021) Genetic re-direction of canine primary T cells for clinical trial use in pet dogs with spontaneous cancer
    STAR Protocols. 2 (4): 100905.
  43. Grudzien, M. et al. (2021) A newly established canine NK-type cell line and its cytotoxic properties.
    Vet Comp Oncol. 19 (3): 567-77.
  44. Yang, Y. et al. (2021) Canine Multicentric Large B Cell Lymphoma with Increased Mott Cells Diagnosed by Flow Cytometry
    Journal of Veterinary Clinics. 38 (1): 36-40.
  45. Enciso, N. et al. (2020) Regenerative potential of allogeneic adipose tissue-derived mesenchymal cells in canine cutaneous wounds.
    Acta Vet Scand. 62 (1): 13.
  46. Lee, S.H. et al. (2021) Safety and immunological effects of recombinant canine IL-15 in dogs.
    Cytokine. 148: 155599.
  47. Knebel, A. et al. (2021) Measurement of canine Th17 cells by flow cytometry.
    Vet Immunol Immunopathol. 243: 110366.
  48. do Prado Duzanski, A. et al. (2022) Cell-mediated immunity and expression of MHC class I and class II molecules in dogs naturally infected by canine transmissible venereal tumor: Is there complete spontaneous regression outside the experimental CTVT?
    Research in Veterinary Science. 145: 193-204.
  49. Troupel, T. et al. (2022) Generalised idiopathic polymyositis mimicking masticatory myositis in a dog
    Vety Rec Case Rep. e452. [Epub ahead of print].

Flow Cytometry

Immunofluorescence

RRID
AB_2291174
UniProt
P27597
Entrez Gene
CD3E
GO Terms
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
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