MHC Class II Monomorphic antibody | IL-A21
FITC conjugated Mouse anti Bovine MHC Class II antibody, clone IL-21A (MCA2445F) used to evaluate MHC class II expression on bovine lymphocytes by flow cytometry.
Image caption:
Gating strategies used to select specific leukocytes subpopulations.
The lymphocytes were identified as R1 based on their size and granularity flow cytometric features prior to the analysis of CD8+, CD4+, CD21+ lymphocytes subsets identified as R2 and proliferation. Lymphocytes subpopulations expressing the memory marker (CD45RO) were quantified based on R3. The mean of fluorescence intensity of MHC class II on lymphocytes subpopulations were quantified based on R4. Percentage of lymphocytes subsets expressing intracytoplasmic cytokines (IFN-γ, IL-17A and IL-4) or cytotoxic markers (perforin and granzyme B) were quantified based on Q1. Percentage of lymphocytes subsets expressing FoxP3-CD25 was determined using quadrant statistics over anti-Foxp3 versus anti-CD25 marker dot plot distribution (Q2). For proliferation assay, the phenotypic analysis was carried to determine the percentage of divided cells using CFSE/anti-bovine surface marker (anti-CD4 or anti-CD8) dot plots.
From: Dorneles EMS, Lima GK, Teixeira-Carvalho A, Araújo MSS, Martins-Filho OA, Sriranganathan N, et al. (2015)
Immune Response of Calves Vaccinated with Brucella abortus S19 or RB51 and Revaccinated with RB51.
PLoS ONE 10(9): e0136696.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Phycoerythrin conjugated Mouse anti Bovine MHC Class II antibody, clone IL-A21 (MCA2445PE) used to evaluate MHC class II expression on bovine dendritic cells by flow cytometry.
Image caption:
Gating strategy for the identification of bovine peripheral blood DC subsets.
Five-color flow cytometry was performed to identify DC subsets in PBMC. Determination of gates was based on fluorescence minus one (FMO) controls for directly labeled antibodies and no first antibody controls when fluorescent secondary antibodies were utilized. Numbers represent percentage of cells in from parent population.
From: Sei JJ, Waters RA, Kenney M, Barlow JW, Golde WT (2016)
Effect of Foot-and-Mouth Disease Virus Infection on the Frequency, Phenotype and Function of Circulating Dendritic Cells in Cattle.
PLoS ONE 11(3): e0152192.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
FITC conjugated Mouse anti Bovine MHC class II antibody, clone IL-A21 (MCA2445PE) used for the evaluation of MHC class II expression on ovine peritoneal cells by flow cytometry.
Image caption:
Percentage of cell populations in the peritoneal fluid during the early stages of infection. The specific cell populations are shown as follows: (a) TCD4; (b) TCD8; (c) WC1+γδ; (d) CD14; (e) MHCII; (f) CD83. Cell subsets of uninfected (Neg. Ctl) and infected sheep (Group 1) are shown in columns. Mann-Whitney U-test was used to compare data from Groups 1 and 3. Values represent the mean ± standard deviation (SD). Asterisks indicate different levels of significance between groups: *P ≤ 0.05, **P ≤ 0.01
From: Pérez-Caballero R, Javier Martínez-Moreno F, Zafra R, Molina-Hernández V, Pacheco IL, Teresa Ruiz-Campillo M, Escamilla A, Pérez J, Martnez-Moreno Á, Buffoni L.
Comparative dynamics of peritoneal cell immunophenotypes in sheep during the early and late stages of the infection with Fasciola hepatica by flow cytometric analysis.
Parasit Vectors. 2018 11(1):640.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Phycoerythrin conjugated Mouse anti Bovine MHC Class II antibody, clone IL-A21 (MCA2445PE) used to evaluate MHC class II expression on bovine dendritic cells by flow cytometry.
Image caption:
Gating strategy for the identification of bovine peripheral blood DC subsets.
Five-color flow cytometry was performed to identify DC subsets in PBMC. Determination of gates was based on fluorescence minus one (FMO) controls for directly labeled antibodies and no first antibody controls when fluorescent secondary antibodies were utilized. Numbers represent percentage of cells in from parent population.
From: Sei JJ, Waters RA, Kenney M, Barlow JW, Golde WT (2016)
Effect of Foot-and-Mouth Disease Virus Infection on the Frequency, Phenotype and Function of Circulating Dendritic Cells in Cattle.
PLoS ONE 11(3): e0152192.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Bovine MHC Class II Monomorphic:Alexa Fluor® 647
Mouse anti Bovine MHC Class II Monomorphic:FITC
Mouse anti Bovine MHC Class II Monomorphic
Mouse anti Bovine MHC Class II Monomorphic:RPE
- Product Type
- Monoclonal Antibody
- Clone
- IL-A21
- Isotype
- IgG2a
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA2445A647 | F | 100 Tests/1ml |
|
|
| MCA2445F | F | 0.1 mg |
|
|
| MCA2445GA | F IP | 0.1 mg |
|
|
| MCA2445PE | F | 100 Tests/1ml |
|
Mouse anti Bovine MHC Class II Monomorphic antibody, clone IL-A21 recognizes a monomorphic epitope within the bovine MHC II cell surface antigen. Clone IL-A21 is reported to react with an epitope which is common to both BoLA DR and DQ (Howard et al.1997).
Expression of MHC II molecules have been demonstrated on bovine B cells, activated T cells, alveolar macrophages, monocytes and mammary and bronchial epithelial cells.
Clone IL-A21 is reported to inhibit T cell proliferation following FMDV infection (Collen et al. 1991).
Expression of MHC II molecules have been demonstrated on bovine B cells, activated T cells, alveolar macrophages, monocytes and mammary and bronchial epithelial cells.
Clone IL-A21 is reported to inhibit T cell proliferation following FMDV infection (Collen et al. 1991).
Product Details
- Target Species
- Bovine
- Product Form
- Purified IgG conjugated to Alexa Fluor 647 - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of MHC Class II Monomorphic antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | |||
| Immunoprecipitation | |||
| Flow Cytometry | Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control:Alexa Fluor® 647 | MCA929A647 | F | 100 Tests/1ml |
|
|
| Mouse IgG2a Negative Control:FITC | MCA929F | F | 100 Tests |
|
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| Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
|
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| Mouse IgG2a Negative Control:RPE | MCA929PE | F | 100 Tests |
|
Product Specific References
References for MHC Class II Monomorphic antibody
-
Collen, T. et al. (1991) A T cell epitope in VP1 of foot-and-mouth disease virus is immunodominant for vaccinated cattle.
J Immunol. 146 (2): 749-55. -
Davies, C.J. et al. (1992) Characterization of bovine MHC class II polymorphism using three typing methods: serology, RFLP and IEF.
Eur J Immunogenet. 19 (5): 253-62. -
Demartini, J.C. et al. (1993) Differential in vitro and in vivo expression of MHC class II antigens in bovine lymphocytes infected by Theileria parva.
Vet Immunol Immunopathol. 35 (3-4): 253-73. -
Howard, C.J. et al. (1997) Identification of two distinct populations of dendritic cells in afferent lymph that vary in their ability to stimulate T cells.
J Immunol. 159 (11): 5372-82. -
Ballingall, K. et al. (2001) Transcription of the unique ruminant class II major histocompatibility complex-DYA and DIB genes in dendritic cells.
Eur J Immunol. 31 (1): 82-6. -
Sathiyaseelan, T. et al. (2002) Immunological characterization of a gammadelta T-cell stimulatory ligand on autologous monocytes.
Immunology. 105:181-9 -
Daubenberger, C. et al. (1999) Bovine gammadelta T-cell responses to the intracellular protozoan parasite Theileria parva
Infect Immun. 67:2241-9. -
Constantinoiu, C.C. et al. (2010) Local immune response against larvae of Rhipicephalus (Boophilus) microplus in Bos taurus indicus and Bos taurus taurus cattle.
Int J Parasitol. 40: 865-75. -
Dorneles, E.M. et al. (2015) Immune Response of Calves Vaccinated with Brucella abortus S19 or RB51 and Revaccinated with RB51.
PLoS One. 10 (9): e0136696. -
Choi, K.S. (2017) The effect of bovine viral diarrhea virus on bovine monocyte phenotype.
Iran J Vet Res. 18 (1): 13-17. -
Sei, J.J. et al. (2016) Effect of Foot-and-Mouth Disease Virus Infection on the Frequency, Phenotype and Function of Circulating Dendritic Cells in Cattle.
PLoS One. 11 (3): e0152192. -
Pérez-caballero, R. et al. (2018) Comparative dynamics of peritoneal cell immunophenotypes in sheep during the early and late stages of the infection with Fasciola hepatica by flow cytometric analysis.
Parasit Vectors. 11 (1): 640. -
Brodzki, P. et al. (2019) Selected leukocyte subpopulations in peripheral blood and uterine washings in cows before and after intrauterine administration of cefapirin and methisoprinol.
Anim Sci J. Nov 06 [Epub ahead of print]. -
Imrie, H. & Williams, D.J.L. (2019) Stimulation of bovine monocyte-derived macrophages with lipopolysaccharide, interferon-γ, Interleukin-4 or Interleukin-13 does not induce detectable changes in nitric oxide or arginase activity.
BMC Vet Res. 15 (1): 45.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸How to Use the Spectraviewer
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