MHC Class II Monomorphic antibody | IL-A21

Mouse anti Bovine MHC Class II Monomorphic

Product Type
Monoclonal Antibody
Clone
IL-A21
Isotype
IgG2a
Specificity
MHC Class II Monomorphic

Product Code Applications Pack Size List Price Your Price Qty
MCA2445GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IP 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Bovine MHC Class II Monomorphic antibody, clone IL-A21 recognizes a monomorphic epitope within the bovine MHC II cell surface antigen. Clone IL-A21 is reported to react with an epitope which is common to both BoLA DR and DQ  (Howard et al.1997).

Expression of MHC II molecules have been demonstrated on bovine B cells, activated T cells, alveolar macrophages, monocytes and mammary and bronchial epithelial cells.

Clone IL-A21 is reported to inhibit T cell proliferation following FMDV infection (Collen et al. 1991).

Target Species
Bovine
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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References for MHC Class II Monomorphic antibody

  1. Collen, T. et al. (1991) A T cell epitope in VP1 of foot-and-mouth disease virus is immunodominant for vaccinated cattle.
    J Immunol. 146 (2): 749-55.
  2. Davies, C.J. et al. (1992) Characterization of bovine MHC class II polymorphism using three typing methods: serology, RFLP and IEF.
    Eur J Immunogenet. 19 (5): 253-62.
  3. Demartini, J.C. et al. (1993) Differential in vitro and in vivo expression of MHC class II antigens in bovine lymphocytes infected by Theileria parva.
    Vet Immunol Immunopathol. 35 (3-4): 253-73.
  4. Howard, C.J. et al. (1997) Identification of two distinct populations of dendritic cells in afferent lymph that vary in their ability to stimulate T cells.
    J Immunol. 159 (11): 5372-82.
  5. Ballingall, K. et al. (2001) Transcription of the unique ruminant class II major histocompatibility complex-DYA and DIB genes in dendritic cells.
    Eur J Immunol. 31 (1): 82-6.
  6. Sathiyaseelan, T. et al. (2002) Immunological characterization of a gammadelta T-cell stimulatory ligand on autologous monocytes.
    Immunology. 105:181-9
  7. Daubenberger, C. et al. (1999) Bovine gammadelta T-cell responses to the intracellular protozoan parasite Theileria parva
    Infect Immun. 67:2241-9.
  8. Constantinoiu, C.C. et al. (2010) Local immune response against larvae of Rhipicephalus (Boophilus) microplus in Bos taurus indicus and Bos taurus taurus cattle.
    Int J Parasitol. 40: 865-75.
  9. View The Latest Product References
  10. Dorneles, E.M. et al. (2015) Immune Response of Calves Vaccinated with Brucella abortus S19 or RB51 and Revaccinated with RB51.
    PLoS One. 10 (9): e0136696.
  11. Choi, K.S. (2017) The effect of bovine viral diarrhea virus on bovine monocyte phenotype.
    Iran J Vet Res. 18 (1): 13-17.
  12. Sei, J.J. et al. (2016) Effect of Foot-and-Mouth Disease Virus Infection on the Frequency, Phenotype and Function of Circulating Dendritic Cells in Cattle.
    PLoS One. 11 (3): e0152192.
  13. Pérez-caballero, R. et al. (2018) Comparative dynamics of peritoneal cell immunophenotypes in sheep during the early and late stages of the infection with Fasciola hepatica by flow cytometric analysis.
    Parasit Vectors. 11 (1): 640.
  14. Brodzki, P. et al. (2020) Selected leukocyte subpopulations in peripheral blood and uterine washings in cows before and after intrauterine administration of cefapirin and methisoprinol.
    Anim Sci J. 91 (1): e13306.
  15. Imrie, H. & Williams, D.J.L. (2019) Stimulation of bovine monocyte-derived macrophages with lipopolysaccharide, interferon-γ, Interleukin-4 or Interleukin-13 does not induce detectable changes in nitric oxide or arginase activity.
    BMC Vet Res. 15 (1): 45.
  16. Marzo, S. et al. (2022) Characterisation of dendritic cell frequency and phenotype in bovine afferent lymph reveals kinetic changes in costimulatory molecule expression
    Vet Immunol Immunopathol. 243: 110363.
  17. Casaro, S. et al. (2022) Flow cytometry panels for immunophenotyping dairy cattle peripheral blood leukocytes
    Vet Immunol Immunopathol. 248: 110417.

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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