MHC Class II Monomorphic antibody | IL-A21

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Anti Bovine MHC Class II Monomorphic Antibody, clone IL-A21 thumbnail image 1

Anti Bovine MHC Class II Monomorphic Antibody, clone IL-A21 thumbnail image 2

Anti Bovine MHC Class II Monomorphic Antibody, clone IL-A21 thumbnail image 3

Anti Bovine MHC Class II Monomorphic Antibody, clone IL-A21 gallery image 1 — Figure A. RPE conjugated Mouse anti Bovine CD1w2 Antibody (MCA2058PE) and FITC conjugated Mouse IgG2a isotype control (MCA929F). Figure B. RPE conjugated Mouse anti Bovine CD1w2 Antibody (MCA2058PE) and FITC conjugated Mouse anti Bovine MHC Class II Monomorphic Antibody (**MCA2445F**). All experiments performed on bovine thymocytes in the presence of 10% bovine serum and gated on live, single cell thymocytes. Data acquired on the ZE5™ Cell Analyzer.

Anti Bovine MHC Class II Monomorphic Antibody, clone IL-A21 gallery image 2 — **Published customer image:**
FITC conjugated Mouse anti Bovine MHC Class II antibody, clone IL-21A (**MCA2445F**) used to evaluate MHC class II expression on bovine lymphocytes by flow cytometry.
**Image caption:**
Gating strategies used to select specific leukocytes subpopulations.
The lymphocytes were identified as R1 based on their size and granularity flow cytometric features prior to the analysis of CD8+, CD4+, CD21+ lymphocytes subsets identified as R2 and proliferation. Lymphocytes subpopulations expressing the memory marker (CD45RO) were quantified based on R3. The mean of fluorescence intensity of MHC class II on lymphocytes subpopulations were quantified based on R4. Percentage of lymphocytes subsets expressing intracytoplasmic cytokines (IFN-γ, IL-17A and IL-4) or cytotoxic markers (perforin and granzyme B) were quantified based on Q1. Percentage of lymphocytes subsets expressing FoxP3-CD25 was determined using quadrant statistics over anti-Foxp3 versus anti-CD25 marker dot plot distribution (Q2). For proliferation assay, the phenotypic analysis was carried to determine the percentage of divided cells using CFSE/anti-bovine surface marker (anti-CD4 or anti-CD8) dot plots.

From: Dorneles EMS, Lima GK, Teixeira-Carvalho A, Araújo MSS, Martins-Filho OA, Sriranganathan N, *et al*. (2015)
Immune Response of Calves Vaccinated with *Brucella abortus* S19 or RB51 and Revaccinated with RB51.
PLoS ONE 10(9): e0136696.

Anti Bovine MHC Class II Monomorphic Antibody, clone IL-A21 gallery image 3 — **Published customer image:**
Phycoerythrin conjugated Mouse anti Bovine MHC Class II antibody, clone IL-A21 (**MCA2445PE**) used to evaluate MHC class II expression on bovine dendritic cells by flow cytometry.
**Image caption:**
Gating strategy for the identification of bovine peripheral blood DC subsets.
Five-color flow cytometry was performed to identify DC subsets in PBMC. Determination of gates was based on fluorescence minus one (FMO) controls for directly labeled antibodies and no first antibody controls when fluorescent secondary antibodies were utilized. Numbers represent percentage of cells in from parent population.

From: Sei JJ, Waters RA, Kenney M, Barlow JW, Golde WT (2016)
Effect of Foot-and-Mouth Disease Virus Infection on the Frequency, Phenotype and Function of Circulating Dendritic Cells in Cattle.
PLoS ONE 11(3): e0152192.

Mouse anti Bovine MHC Class II Monomorphic:FITC

Mouse anti Bovine MHC Class II Monomorphic

Mouse anti Bovine MHC Class II Monomorphic:RPE

Product Type: Monoclonal Antibody
Clone: IL-A21
Isotype: IgG2a

Product Code	Applications	Pack Size
MCA2445F	F	0.1 mg
MCA2445GA	F IP	0.1 mg
MCA2445PE	F	100 Tests/1ml

Mouse anti Bovine MHC Class II Monomorphic antibody, clone IL-A21 recognizes a monomorphic epitope within the bovine MHC II cell surface antigen. Clone IL-A21 is reported to react with an epitope which is common to both BoLA DR and DQ (Howard et al.1997).

Expression of MHC II molecules have been demonstrated on bovine B cells, activated T cells, alveolar macrophages, monocytes and mammary and bronchial epithelial cells.

Clone IL-A21 is reported to inhibit T cell proliferation following FMDV infection.

Product Details

Target Species: Bovine
Product Form: Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form: Purified IgG - liquid
Product Form: Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution: Reconstitute with 1.0 ml distilled water
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
5% Sucrose
Carrier Free: Yes
Approx. Protein Concentrations: IgG concentration 0.1 mg/ml
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Prior to reconstitution store at +4^oC.
After reconstitution store at +4^oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Shelf Life: 12 months from date of reconstitution.

More Information

Regulatory: For research purposes only

Applications of MHC Class II Monomorphic antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry	Neat	1/10
Flow Cytometry
Immunoprecipitation
Flow Cytometry	Neat

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Human anti Mouse IgG2a:FITC	HCA037F	0.1 mg	F
Human anti Mouse IgG2a:HRP	HCA037P	0.1 mg	E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications
Mouse IgG2a Negative Control:FITC	MCA929F	100 Tests	F
Mouse IgG2a Negative Control	MCA929	100 Tests	F
Mouse IgG2a Negative Control:RPE	MCA929PE	100 Tests	F

Product Specific References

References for MHC Class II Monomorphic antibody

Collen, T. et al. (1991) A T cell epitope in VP1 of foot-and-mouth disease virus is immunodominant for vaccinated cattle.
J Immunol. 146 (2): 749-55.
Davies, C.J. et al. (1992) Characterization of bovine MHC class II polymorphism using three typing methods: serology, RFLP and IEF.
Eur J Immunogenet. 19 (5): 253-62.
Demartini, J.C. et al. (1993) Differential in vitro and in vivo expression of MHC class II antigens in bovine lymphocytes infected by Theileria parva.
Vet Immunol Immunopathol. 35 (3-4): 253-73.
Howard, C.J. et al. (1997) Identification of two distinct populations of dendritic cells in afferent lymph that vary in their ability to stimulate T cells.
J Immunol. 159 (11): 5372-82.
Ballingall, K. et al. (2001) Transcription of the unique ruminant class II major histocompatibility complex-DYA and DIB genes in dendritic cells.
Eur J Immunol. 31 (1): 82-6.
Sathiyaseelan, T. et al. (2002) Immunological characterization of a gammadelta T-cell stimulatory ligand on autologous monocytes.
Immunology. 105:181-9
Daubenberger, C. et al. (1999) Bovine gammadelta T-cell responses to the intracellular protozoan parasite Theileria parva
Infect Immun. 67:2241-9.
Constantinoiu, C.C. et al. (2010) Local immune response against larvae of Rhipicephalus (Boophilus) microplus in Bos taurus indicus and Bos taurus taurus cattle.
Int J Parasitol. 40: 865-75.
Dorneles, E.M. et al. (2015) Immune Response of Calves Vaccinated with Brucella abortus S19 or RB51 and Revaccinated with RB51.
PLoS One. 10 (9): e0136696.
Choi, K.S. (2017) The effect of bovine viral diarrhea virus on bovine monocyte phenotype.
Iran J Vet Res. 18 (1): 13-17.
Sei, J.J. et al. (2016) Effect of Foot-and-Mouth Disease Virus Infection on the Frequency, Phenotype and Function of Circulating Dendritic Cells in Cattle.
PLoS One. 11 (3): e0152192.

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