MHC Class II Monomorphic antibody | IL-A21

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Mouse anti Bovine MHC Class II Monomorphic:FITC

Mouse anti Bovine MHC Class II Monomorphic

Mouse anti Bovine MHC Class II Monomorphic:RPE

Product Type
Monoclonal Antibody
Clone
IL-A21
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2445F F 0.1 mg
MCA2445GA F IP 0.1 mg
MCA2445PE F 100 Tests/1ml
Mouse anti Bovine MHC Class II Monomorphic antibody, clone IL-A21 recognizes a monomorphic epitope within the bovine MHC II cell surface antigen. Clone IL-A21 is reported to react with an epitope which is common to both BoLA DR and DQ  (Howard et al.1997).

Expression of MHC II molecules have been demonstrated on bovine B cells, activated T cells, alveolar macrophages, monocytes and mammary and bronchial epithelial cells.

Clone IL-A21 is reported to inhibit T cell proliferation following FMDV infection.

Product Details

Target Species
Bovine
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of reconstitution.

More Information

Regulatory
For research purposes only

Applications of MHC Class II Monomorphic antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry
Immunoprecipitation
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG2a Negative Control:FITC MCA929F 100 Tests F
Mouse IgG2a Negative Control MCA929 100 Tests F
Mouse IgG2a Negative Control:RPE MCA929PE 100 Tests F

Product Specific References

References for MHC Class II Monomorphic antibody

  1. Collen, T. et al. (1991) A T cell epitope in VP1 of foot-and-mouth disease virus is immunodominant for vaccinated cattle.
    J Immunol. 146 (2): 749-55.
  2. Davies, C.J. et al. (1992) Characterization of bovine MHC class II polymorphism using three typing methods: serology, RFLP and IEF.
    Eur J Immunogenet. 19 (5): 253-62.
  3. Demartini, J.C. et al. (1993) Differential in vitro and in vivo expression of MHC class II antigens in bovine lymphocytes infected by Theileria parva.
    Vet Immunol Immunopathol. 35 (3-4): 253-73.
  4. Howard, C.J. et al. (1997) Identification of two distinct populations of dendritic cells in afferent lymph that vary in their ability to stimulate T cells.
    J Immunol. 159 (11): 5372-82.
  5. Ballingall, K. et al. (2001) Transcription of the unique ruminant class II major histocompatibility complex-DYA and DIB genes in dendritic cells.
    Eur J Immunol. 31 (1): 82-6.
  6. Sathiyaseelan, T. et al. (2002) Immunological characterization of a gammadelta T-cell stimulatory ligand on autologous monocytes.
    Immunology. 105:181-9
  7. Daubenberger, C. et al. (1999) Bovine gammadelta T-cell responses to the intracellular protozoan parasite Theileria parva
    Infect Immun. 67:2241-9.
  8. Constantinoiu, C.C. et al. (2010) Local immune response against larvae of Rhipicephalus (Boophilus) microplus in Bos taurus indicus and Bos taurus taurus cattle.
    Int J Parasitol. 40: 865-75.
  9. Dorneles, E.M. et al. (2015) Immune Response of Calves Vaccinated with Brucella abortus S19 or RB51 and Revaccinated with RB51.
    PLoS One. 10 (9): e0136696.
  10. Choi, K.S. (2017) The effect of bovine viral diarrhea virus on bovine monocyte phenotype.
    Iran J Vet Res. 18 (1): 13-17.
  11. Sei, J.J. et al. (2016) Effect of Foot-and-Mouth Disease Virus Infection on the Frequency, Phenotype and Function of Circulating Dendritic Cells in Cattle.
    PLoS One. 11 (3): e0152192.