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MHC Class II Monomorphic antibody | IL-A21

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Anti Bovine MHC Class II Monomorphic Antibody, clone IL-A21 thumbnail image 1 Anti Bovine MHC Class II Monomorphic Antibody, clone IL-A21 thumbnail image 2 Anti Bovine MHC Class II Monomorphic Antibody, clone IL-A21 thumbnail image 3 Anti Bovine MHC Class II Monomorphic Antibody, clone IL-A21 thumbnail image 4 Anti Bovine MHC Class II Monomorphic Antibody, clone IL-A21 thumbnail image 5
Anti Bovine MHC Class II Monomorphic Antibody, clone IL-A21 gallery image 1
Figure A. RPE conjugated Mouse anti Bovine CD1w2 Antibody (MCA2058PE) and FITC conjugated Mouse IgG2a isotype control (MCA929F). Figure B. RPE conjugated Mouse anti Bovine CD1w2 Antibody (MCA2058PE) and FITC conjugated Mouse anti Bovine MHC Class II Monomorphic Antibody (MCA2445F). All experiments performed on bovine thymocytes in the presence of 10% bovine serum and gated on live, single cell thymocytes. Data acquired on the ZE5™ Cell Analyzer.
Anti Bovine MHC Class II Monomorphic Antibody, clone IL-A21 gallery image 2
Published customer image:
FITC conjugated Mouse anti Bovine MHC Class II antibody, clone IL-21A (MCA2445F) used to evaluate MHC class II expression on bovine lymphocytes by flow cytometry.
Image caption:
Gating strategies used to select specific leukocytes subpopulations.
The lymphocytes were identified as R1 based on their size and granularity flow cytometric features prior to the analysis of CD8+, CD4+, CD21+ lymphocytes subsets identified as R2 and proliferation. Lymphocytes subpopulations expressing the memory marker (CD45RO) were quantified based on R3. The mean of fluorescence intensity of MHC class II on lymphocytes subpopulations were quantified based on R4. Percentage of lymphocytes subsets expressing intracytoplasmic cytokines (IFN-γ, IL-17A and IL-4) or cytotoxic markers (perforin and granzyme B) were quantified based on Q1. Percentage of lymphocytes subsets expressing FoxP3-CD25 was determined using quadrant statistics over anti-Foxp3 versus anti-CD25 marker dot plot distribution (Q2). For proliferation assay, the phenotypic analysis was carried to determine the percentage of divided cells using CFSE/anti-bovine surface marker (anti-CD4 or anti-CD8) dot plots.

From: Dorneles EMS, Lima GK, Teixeira-Carvalho A, Araújo MSS, Martins-Filho OA, Sriranganathan N, et al. (2015)
Immune Response of Calves Vaccinated with Brucella abortus S19 or RB51 and Revaccinated with RB51.
PLoS ONE 10(9): e0136696.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Anti Bovine MHC Class II Monomorphic Antibody, clone IL-A21 gallery image 3
Published customer image:
Phycoerythrin conjugated Mouse anti Bovine MHC Class II antibody, clone IL-A21 (MCA2445PE) used to evaluate MHC class II expression on bovine dendritic cells by flow cytometry.
Image caption:
Gating strategy for the identification of bovine peripheral blood DC subsets.
Five-color flow cytometry was performed to identify DC subsets in PBMC. Determination of gates was based on fluorescence minus one (FMO) controls for directly labeled antibodies and no first antibody controls when fluorescent secondary antibodies were utilized. Numbers represent percentage of cells in from parent population.

From: Sei JJ, Waters RA, Kenney M, Barlow JW, Golde WT (2016)
Effect of Foot-and-Mouth Disease Virus Infection on the Frequency, Phenotype and Function of Circulating Dendritic Cells in Cattle.
PLoS ONE 11(3): e0152192.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Anti Bovine MHC Class II Monomorphic Antibody, clone IL-A21 gallery image 4
Published customer image:
FITC conjugated Mouse anti Bovine MHC class II antibody, clone IL-A21 (MCA2445PE) used for the evaluation of MHC class II expression on ovine peritoneal cells by flow cytometry.
Image caption:
Percentage of cell populations in the peritoneal fluid during the early stages of infection. The specific cell populations are shown as follows: (a) TCD4; (b) TCD8; (c) WC1+γδ; (d) CD14; (e) MHCII; (f) CD83. Cell subsets of uninfected (Neg. Ctl) and infected sheep (Group 1) are shown in columns. Mann-Whitney U-test was used to compare data from Groups 1 and 3. Values represent the mean ± standard deviation (SD). Asterisks indicate different levels of significance between groups: *P ≤ 0.05, **P ≤ 0.01

From: Pérez-Caballero R, Javier Martínez-Moreno F, Zafra R, Molina-Hernández V, Pacheco IL, Teresa Ruiz-Campillo M, Escamilla A, Pérez J, Martnez-Moreno Á, Buffoni L.
Comparative dynamics of peritoneal cell immunophenotypes in sheep during the early and late stages of the infection with Fasciola hepatica by flow cytometric analysis.
Parasit Vectors. 2018 11(1):640.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Anti Bovine MHC Class II Monomorphic Antibody, clone IL-A21 gallery image 5
Published customer image:
Phycoerythrin conjugated Mouse anti Bovine MHC Class II antibody, clone IL-A21 (MCA2445PE) used to evaluate MHC class II expression on bovine dendritic cells by flow cytometry.
Image caption:
Gating strategy for the identification of bovine peripheral blood DC subsets.
Five-color flow cytometry was performed to identify DC subsets in PBMC. Determination of gates was based on fluorescence minus one (FMO) controls for directly labeled antibodies and no first antibody controls when fluorescent secondary antibodies were utilized. Numbers represent percentage of cells in from parent population.

From: Sei JJ, Waters RA, Kenney M, Barlow JW, Golde WT (2016)
Effect of Foot-and-Mouth Disease Virus Infection on the Frequency, Phenotype and Function of Circulating Dendritic Cells in Cattle.
PLoS ONE 11(3): e0152192.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.

Mouse anti Bovine MHC Class II Monomorphic:FITC

Mouse anti Bovine MHC Class II Monomorphic

Mouse anti Bovine MHC Class II Monomorphic:RPE

Product Type
Monoclonal Antibody
Clone
IL-A21
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA2445F
Datasheet
F
SDS
0.1 mg loader

MCA2445GA
Datasheet
F IP
SDS
0.1 mg loader

MCA2445PE
Datasheet
F
SDS
100 Tests/1ml loader

Mouse anti Bovine MHC Class II Monomorphic antibody, clone IL-A21 recognizes a monomorphic epitope within the bovine MHC II cell surface antigen. Clone IL-A21 is reported to react with an epitope which is common to both BoLA DR and DQ  (Howard et al.1997).

Expression of MHC II molecules have been demonstrated on bovine B cells, activated T cells, alveolar macrophages, monocytes and mammary and bronchial epithelial cells.

Clone IL-A21 is reported to inhibit T cell proliferation following FMDV infection (Collen et al. 1991).

Product Details

Target Species
Bovine
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of MHC Class II Monomorphic antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry
Immunoprecipitation
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgG2a:HRP HCA037P E 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG2a Negative Control:FITC MCA929F F 100 Tests loader
Mouse IgG2a Negative Control MCA929 F 100 Tests loader
Mouse IgG2a Negative Control:RPE MCA929PE F 100 Tests loader

Product Specific References

References for MHC Class II Monomorphic antibody

  1. Collen, T. et al. (1991) A T cell epitope in VP1 of foot-and-mouth disease virus is immunodominant for vaccinated cattle.
    J Immunol. 146 (2): 749-55.
  2. Davies, C.J. et al. (1992) Characterization of bovine MHC class II polymorphism using three typing methods: serology, RFLP and IEF.
    Eur J Immunogenet. 19 (5): 253-62.
  3. Demartini, J.C. et al. (1993) Differential in vitro and in vivo expression of MHC class II antigens in bovine lymphocytes infected by Theileria parva.
    Vet Immunol Immunopathol. 35 (3-4): 253-73.
  4. Howard, C.J. et al. (1997) Identification of two distinct populations of dendritic cells in afferent lymph that vary in their ability to stimulate T cells.
    J Immunol. 159 (11): 5372-82.
  5. Ballingall, K. et al. (2001) Transcription of the unique ruminant class II major histocompatibility complex-DYA and DIB genes in dendritic cells.
    Eur J Immunol. 31 (1): 82-6.
  6. Sathiyaseelan, T. et al. (2002) Immunological characterization of a gammadelta T-cell stimulatory ligand on autologous monocytes.
    Immunology. 105:181-9
  7. Daubenberger, C. et al. (1999) Bovine gammadelta T-cell responses to the intracellular protozoan parasite Theileria parva
    Infect Immun. 67:2241-9.
  8. Constantinoiu, C.C. et al. (2010) Local immune response against larvae of Rhipicephalus (Boophilus) microplus in Bos taurus indicus and Bos taurus taurus cattle.
    Int J Parasitol. 40: 865-75.
  9. Dorneles, E.M. et al. (2015) Immune Response of Calves Vaccinated with Brucella abortus S19 or RB51 and Revaccinated with RB51.
    PLoS One. 10 (9): e0136696.
  10. Choi, K.S. (2017) The effect of bovine viral diarrhea virus on bovine monocyte phenotype.
    Iran J Vet Res. 18 (1): 13-17.
  11. Sei, J.J. et al. (2016) Effect of Foot-and-Mouth Disease Virus Infection on the Frequency, Phenotype and Function of Circulating Dendritic Cells in Cattle.
    PLoS One. 11 (3): e0152192.
  12. Pérez-caballero, R. et al. (2018) Comparative dynamics of peritoneal cell immunophenotypes in sheep during the early and late stages of the infection with Fasciola hepatica by flow cytometric analysis.
    Parasit Vectors. 11 (1): 640.
  13. Brodzki, P. et al. (2019) Selected leukocyte subpopulations in peripheral blood and uterine washings in cows before and after intrauterine administration of cefapirin and methisoprinol.
    Anim Sci J. Nov 06 [Epub ahead of print].
  14. Imrie, H. & Williams, D.J.L. (2019) Stimulation of bovine monocyte-derived macrophages with lipopolysaccharide, interferon-γ, Interleukin-4 or Interleukin-13 does not induce detectable changes in nitric oxide or arginase activity.
    BMC Vet Res. 15 (1): 45.

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