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CD1w2 antibody | CC20

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Anti Bovine CD1w2 Antibody, clone CC20 thumbnail image 1 Anti Bovine CD1w2 Antibody, clone CC20 thumbnail image 2 Anti Bovine CD1w2 Antibody, clone CC20 thumbnail image 3 Anti Bovine CD1w2 Antibody, clone CC20 thumbnail image 4 Anti Bovine CD1w2 Antibody, clone CC20 thumbnail image 5
Anti Bovine CD1w2 Antibody, clone CC20 gallery image 1
Figure A. FITC conjugated Mouse anti Bovine MHC Class II Monomorphic Antibody (MCA2445F) and RPE conjugated Mouse IgG2a isotype control (MCA929PE). Figure B. FITC conjugated Mouse anti Bovine MHC Class II Monomorphic Antibody (MCA2445F) and RPE conjugated Mouse anti Bovine CD1w2 Antibody (MCA2058PE). All experiments performed on bovine thymocytes in the presence of 10% bovine serum and gated on live, single cell thymocytes. Data acquired on the ZE5 Cell Analyzer.
Anti Bovine CD1w2 Antibody, clone CC20 gallery image 2
Figure A. FITC conjugated Mouse anti Bovine CD4 Antibody (MCA1653F) and Mouse IgG2a isotype control (MCA929) conjugated with Rabbit F(ab')2 anti Mouse IgG:RPE Antibody (STAR12A). Figure B. FITC conjugated Mouse anti Bovine CD4 Antibody (MCA1653F) and Mouse anti Bovine CD1w2 Antibody (MCA2058GA) conjugated with Rabbit F(ab')2 anti Mouse IgG:RPE Antibody (STAR12A). All experiments performed on bovine thymocytes in the presence of 10% bovine serum and gated on live, single cell thymocytes. Data acquired on the ZE5 Cell Analyzer.
Anti Bovine CD1w2 Antibody, clone CC20 gallery image 3
Figure A. FITC conjugated Mouse anti Bovine CD4 Antibody (MCA1653F) and RPE conjugated Mouse IgG2a isotype control (MCA929PE). Figure B. FITC conjugated Mouse anti Bovine CD4 Antibody (MCA1653F) and RPE conjugated Mouse anti Bovine CD1w2 Antibody (MCA2058PE). All experiments performed on bovine thymocytes in the presence of 10% bovine serum and gated on live, single cell thymocytes. Data acquired on the ZE5 Cell Analyzer.
Anti Bovine CD1w2 Antibody, clone CC20 gallery image 4
Figure A. FITC conjugated Mouse anti Bovine MHC Class II Monomorphic Antibody (MCA2445F) and Mouse IgG2a isotype control (MCA929) conjugated with Rabbit F(ab')2 anti Mouse IgG:RPE Antibody (STAR12A). Figure B. FITC conjugated Mouse anti Bovine MHC Class II Monomorphic Antibody (MCA2445F) and Mouse anti Bovine CD1w2 Antibody (MCA2058GA) conjugated with Rabbit F(ab')2 anti Mouse IgG:RPE Antibody (STAR12A). All experiments performed on bovine thymocytes in the presence of 10% bovine serum and gated on live, single cell thymocytes. Data acquired on the ZE5 Cell Analyzer.
Anti Bovine CD1w2 Antibody, clone CC20 gallery image 5
Detection of Mouse anti Bovine CD1w2 (MCA2058GA) in an immunohistochemical analysis on acetone-fixed frozen bovine thymus. Original magnification x60.

Mouse anti Bovine CD1w2:RPE

Product Type
Monoclonal Antibody
Clone
CC20
Isotype
IgG2a
Specificity
CD1w2

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Product Code Applications Pack Size List Price Your Price Qty
MCA2058PE
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests loader
List Price Your Price
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Mouse anti Bovine CD1w2 antibody, clone CC20 recognises the bovine CD1w2 cell surface antigen, a glycoprotein heterodimer of ~12 kDa and ~46 kDa. CD1w2 is expressed by dendritic cells, cortical thymocytes and a minority of medullary thymocytes, with a pattern similar to antibodies of the CD1b cluster in humans.

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep
Goat
Dog
Horse
Cat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose
Fusion Partners
Spleen cells from immunised mice were fused with cells of the mouse NS1 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4°C.
After reconstitution store at +4°C.
DO NOT FREEZE.
This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/5
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

How to Use the Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG2a Negative Control:RPE MCA929PE F 100 Tests
>
List Price Your Price
Description Mouse IgG2a Negative Control:RPE

References for CD1w2 antibody

  1. Hein, W.R. et al. (1991) Summary of workshop findings for leukocyte antigens of sheep.
    Vet Immunol Immunopathol. 27 (1-3): 28-30.
  2. Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle (tables 1 and 2).
    Vet Immunol Immunopathol. 39 (1-3): 25-47.
  3. Howard, C.J. et al. (1993) Comparison of CD1 monoclonal antibodies on bovine cells and tissues.
    Vet Immunol Immunopathol. 39 (1-3): 77-83.
  4. Moore, P.F. et al. (1996) Canine cutaneous histiocytoma is an epidermotropic Langerhans cell histiocytosis that expresses CD1 and specific beta 2-integrin molecules.
    Am J Pathol. 148: 1699-708.
  5. Siedek, E. et al. (1997) Isolation and characterisation of equine dendritic cells.
    Vet Immunol Immunopathol. 60 (1-2): 15-31.
  6. Rhind, S.M. (2001) CD1--the pathology perspective.
    Vet Pathol. 38 (6): 611-9.
  7. Affolter, V.K. and Moore, P.F. (2002) Localized and disseminated histiocytic sarcoma of dendritic cell origin in dogs.
    Vet Pathol. 39: 74-83.
  8. Chan, S.S. et al. (2002) Generation and characterization of ovine dendritic cells derived from peripheral blood monocytes.
    Immunology. 107: 366-72.
    9. View The Latest Product References
  9. Bienzle, D. et al. (2003) Immunophenotype and functional properties of feline dendritic cells derived from blood and bone marrow.
    Vet Immunol Immunopathol. 96: 19-30.
  10. McNeilly, T.N. et al. (2006) Differential expression of cell surface markers by ovine respiratory tract dendritic cells.
    J Histochem Cytochem. 54: 1021-30.
  11. Åkesson, C.P. et al. (2008) Phenotypic characterisation of intestinal dendritic cells in sheep.
    Dev Comp Immunol. 32: 837-49.
  12. Mérant, C. et al. (2009) Young foal and adult horse monocyte-derived dendritic cells differ by their degree of phenotypic maturity.
    Vet Immunol Immunopathol. 131: 1-8.
  13. Shu, D. et al. (2009) Cutaneous cytokine gene expression and cellular responses in lambs infested with the louse, Bovicola ovis, and following intradermal injection of crude louse antigen.
    Vet Immunol Immunopathol. 129: 82-92.
  14. Romero-palomo, F. et al. (2013) Immunohistochemical detection of dendritic cell markers in cattle.
    Vet Pathol. 50 (6): 1099-108.
  15. Romero-Palomo, F. et al. (2017) Immunopathologic Changes in the Thymus of Calves Pre-infected with BVDV and Challenged with BHV-1.
    Transbound Emerg Dis. 64 (2): 574-84.

Flow Cytometry

Synonyms
CD1b
RRID
AB_609584

MCA2058PE

154032 167386 INN1609

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