IFN Gamma antibody | CC302
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Mouse anti Bovine Interferon Gamma
- Product Type
- Monoclonal Antibody
- IFN Gamma
|Mouse anti Bovine IFNγ antibody, clone CC302 recognizes bovine interferon-gamma, a 143 amino acid cytokine with potent activating, antiviral and anti proliferative properties, produced as a pro-peptide with an additional 23 amino acid N-terminal signal peptide sequence having a molecular weight of ~20 kDa. IFNγ is predominantly secreted by activated T lymphocytes in response to specific mitogens as a result of infection (Rhodes et al. 2000).
Mouse anti bovine γ interferon antibody, clone CC302 has been demonstrated to be reactive to a number of mammalian species including human, sheep, dog, pig, goat and mink (Pedersen et al. 2002). Mouse anti Bovine IFNγ antibody, clone CC302 has been used successfully for the evaluation of γ interferon levels in the sera of calves naturally infected with M. avium. subsp paratuberculosis (Appana et al. 2013) as a detection reagent using an ELISA.
Our IFN Gamma (CC302) Antibody has been referenced in >83 publications*
*Based on June 2020 data from CiteAb's antibody search engine.
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Human Pig Dog Horse Sheep Mustelid Expected from Sequence Goat Dolphin Ferret Mink Fin Whale Rabbit
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Flow Cytometry 1||1/100||1/500|
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1x106 cells in 100μl
- Biotinylated mouse anti bovine IFNγ, clone CC302, may be used as the detection reagent in a sandwich ELISA with purified mouse anti bovine IFNγ, clone CC330, as the capture reagent and recombinant bovine IFNγ as the standard.
|Description||Product Code||Applications||Pack Size||List Price||Your Price||Quantity|
|Mouse IgG1 Negative Control||MCA928||F||100 Tests||Log in|
|List Price||Your Price|
|Description||Mouse IgG1 Negative Control|
References for IFN Gamma antibody
Hasvold, H.J. et al. (2002) In vitro responses to purified protein derivate of caprine T lymphocytes following vaccination with live strains of Mycobacterium avium subsp paratuberculosis.
Vet Immunol Immunopathol. 90 (1-2): 79-89.
Mwangi, W. et al. (2002) DNA-encoded fetal liver tyrosine kinase 3 ligand and granulocyte macrophage-colony-stimulating factor increase dendritic cell recruitment to the inoculation site and enhance antigen-specific CD4+ T cell responses induced by DNA vaccination of outbred animals.
J Immunol. 169 (7): 3837-46.
Pedersen, L.G. et al. (2002) Identification of monoclonal antibodies that cross-react with cytokines from different animal species.
Vet Immunol Immunopathol. 88 (3-4): 111-22.
Aasted, B. et al. (2002) Cytokine profiles in peripheral blood mononuclear cells and lymph node cells from piglets infected in utero with porcine reproductive and respiratory syndrome virus.
Clin Diagn Lab Immunol. 9 (6): 1229-34.
Cameron, C.M. et al. (2005) Myxoma virus M141R expresses a viral CD200 (vOX-2) that is responsible for down-regulation of macrophage and T-cell activation in vivo.
J Virol. 79: 6052-67.
Reber, A.J. et al. (2006) Evaluation of multiple immune parameters after vaccination with modified live or killed bovine viral diarrhea virus vaccines.
Comp Immunol Microbiol Infect Dis. 29 (1): 61-77.
Katepalli, M.P. et al. (2008) The effect of age and telomere length on immune function in the horse.
Dev Comp Immunol. 32 (12): 1409-15.
Johnson, W.C. et al. (2008) Bovine WC1(-) gammadeltaT cells incubated with IL-15 express the natural cytotoxicity receptor CD335 (NKp46) and produce IFN-gamma in response to exogenous IL-12 and IL-18.
Dev Comp Immunol. 32 (8): 1002-10.
View The Latest Product References
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Papadogiannakis, E.I. et al. (2009) Determination of intracellular cytokines IFN-γ and IL-4 in canine T lymphocytes by flow cytometry following whole-blood culture.
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Jensen, T.H. et al. (2009) Early life DNA vaccination with the H gene of Canine distemper virus induces robust protection against distemper.
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Martel, C.J. & Aasted, B. (2009) Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers.
Vet Immunol Immunopathol. 132:109-15.
Lybeck, K.R. et al. (2009) Neutralization of interleukin-10 from CD14(+) monocytes enhances gamma interferon production in peripheral blood mononuclear cells from Mycobacterium avium subsp. paratuberculosis-infected goats.
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Elhmouzi-Younes, J. et al. (2009) Bovine neonate natural killer cells are fully functional and highly responsive to interleukin-15 and to NKp46 receptor stimulation.
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Fellman, C.L. et al. (2011) Cyclosporine A affects the in vitro expression of T cell activation-related molecules and cytokines in dogs.
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Sow, F.B. et al. (2011) Respiratory syncytial virus is associated with an inflammatory response in lungs and architectural remodeling of lung-draining lymph nodes of newborn lambs.
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Skyberg, J.A. et al. (2011) Murine and bovine γδ T cells enhance innate immunity against Brucella abortus infections.
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Pillet, S. et al. (2011) Cellular immune response in the presence of protective antibody levels correlates with protection against 1918 influenza in ferrets.
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Summers, C. et al. (2012) The distribution of immune cells in the lungs of classical and atypical ovine pulmonary adenocarcinoma.
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Maślanka T et al. (2012) The presence of CD25 on bovine WC1+ γδ T cells is positively correlated with their production of IL-10 and TGF-β, but not IFN-γ.
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Sun, L. et al. (2012) The role of proliferation in the regulation of interferon gamma (IFNγ) expression in foals.
Dev Comp Immunol. 36 (3): 534-9.
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Hedges, J.F. et al. (2015) Amphotericin B stimulates γδ T and NK cells, and enhances protection from Salmonella infection.
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Boshra H et al. (2015) A lumpy skin disease virus deficient of an IL-10 gene homologue provides protective immunity against virulent capripoxvirus challenge in sheep and goats.
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- Interferon Gamma
- Entrez Gene
- GO Terms
- GO:0009615 response to virus
- GO:0005125 cytokine activity
- GO:0005133 interferon-gamma receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
MCA1783159416 164179 165207 1711
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