IFN Gamma Antibody | CC330

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IFN Gamma Antibody | CC330 gallery image 1

Published customer image:
IFN-gamma response (log10 scale) in mesenteric lymph nodes, Peyer’s Patches, popliteal LN and PBMC against GRA7, TLA and MIC3 in function of time.

From: Verhelst D, De Craeye S, Entrican G, Dorny P, Cox E. Parasite distribution and associated immune response during the acute phase of Toxoplasma gondii infection in sheep. BMC Vet Res. 2014 Dec 16;10(1):293.

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γδ T cells are the primary source of IL-17 during B. abortus infection. C57BL/6 mice were infected i.p. with 5×104 CFUs of B. abortus 2308, and two weeks later γδ T cells (>95% purity) and an enriched TCRαβ (~55% CD4+, 25% CD8+) cell fraction were isolated from the spleens of infected mice. Cells were stimulated with 500 ng/ml ionomycin and 50 ng/ml PMA for three days, and cell-free supernatants from triplicate wells were assayed for cytokine production via ELISA. The mean ± SD is shown; * P<0.05 versus the enriched TCRαβ cells. Results are representative of two independent experiments.

From: Skyberg JA, Thornburg T, Rollins M, Huarte E, Jutila MA, et al. (2011) Murine and Bovine ?d T Cells Enhance Innate Immunity against Brucella abortus Infections. PLoS ONE 6(7): e21978.

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γδ T cells require TNF-α to protect against B. abortus infection. C57BL/6 mice treated with anti-TCRγδ mAb or hamster IgG on day -1 and day 3 post-infection with 5×104 CFUs of B. abortus 2308. Some mice were also neutralized of their TNF-α on days -1 and 3. Seven days after infection, A. splenic weights and B. extent of brucellae colonization were determined. The mean ± SEM of 10 mice/group is depicted; *P<0.05 versus hamster IgG-treated C57BL/6 mice. Results are from two independent experiments. C. and D. Splenocytes (5×106/ml) from 4 or 7 day B. abortus-infected mice depleted or not of γδ T cells and/or TNF-a or splenocytes from naive C57BL/6 mice were left unstimulated and cultured for 3 days at 37°C/5%CO2; supernatants were harvested for C. TNF-α- or D. IFN-γ- specific ELISA. The mean ± SD of triplicate wells is shown. The results from 7 dpi are representative of two independent experiments. NS = not significant. ND = cytokine production from uninfected mice neutralized of γδ T cells was not determined. * P<0.05 as compared to mice not depleted of γδ T cells within the same TNF-α treatment group at the same time point. † P<0.05 as compared to C57BL/6 mice treated with IgG only at the same time point. E. The median fluorescence intensity (MFI) of CD69 expression by splenic NK and γδ T cells as measured by flow cytometry is shown for naïve C57BL/6 and B. abortus-infected (after 4 days of infection with 5×104 CFUs of strain 2308) C57BL/6 mice. Data depict the mean ± SD from 5 mice/group; *P<0.05 versus naïve mice and † P<0.05 versus B. abortus-infected mice not neutralized of their TNF-α. F. and G. Peritoneal macrophages from C57BL/6 and TCRδ-/- mice were infected with B. abortus (30 bacteria:1 macrophage), and F. TNF-α levels in supernatants and G. intracellular colonization were measured. Data represent the mean ± SD of triplicate wells/group.

From: Skyberg JA, Thornburg T, Rollins M, Huarte E, Jutila MA, et al. (2011) Murine and Bovine γδ T Cells Enhance Innate Immunity against Brucella abortus Infections. PLoS ONE 6(7): e21978.

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Bovine γδ T cells impair B. abortus replication in autologous macrophages via IFN-γ. A.–F. Bovine macrophages were infected with B. abortus (30 bacteria:1 macrophage) and then fresh media or media containing autologous γδ T cells were added to infected macrophages. A., C., E. Macrophage colonization (triplicate wells/treatment) was monitored over time. *P<0.05 versus colonization by cultured macrophages only from the same animal. B.,D.,F. IFN-γ levels were determined in cell culture supernatants. At 72 and 120 h post-infection, macrophage plus γδ T cell co-cultures from each calf contained more IFN-γ than from macrophages cultured alone. G. NK1.1+ cell-depleted Rag-1-/- mice (4–5 per group) received PBS, bovine macrophages (5×105/mouse), bovine macrophages (5×105/mouse) plus autologous γδ T cells (1×107/mouse), or bovine macrophages plus autologous CD4+ T cells (1×107/mouse) i.p. one day prior to infection with 1×104 CFUs of B. abortus. All cells were derived from Calf #1. On day 7, splenic colonization was determined. Depicted is the mean ± SD; *P<0.05 versus mice given PBS or macrophages only.

From: Skyberg JA, Thornburg T, Rollins M, Huarte E, Jutila MA, et al. (2011) Murine and Bovine γδ T Cells Enhance Innate Immunity against Brucella abortus Infections. PLoS ONE 6(7): e21978.

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Mouse anti bovine interferon gamma antibody, clone CC330 used for the measurement of the gamma interferon response to M. avium infection in a caprine model by ELISA in conjunction with biotinylated Mouse anti Bovine interferon γ antibody, clone CC302 as a detection reagent.
Image caption:
Time course and intensity of MAP-specific antibody response (A) and antigen-induced (Johnin, 4 µg/mL) IFN-? response (B) of inoculated and control goats. Box and Whisker Plot represents median value, 25% and 75% percentiles (box), range, outlier values (?), and extreme values (*). Different letters indicate significant differences between groups (Mann–Whitney-U test, P?=?0.05): a – V1 vs. V2, b – V1 vs. V3, c – V1 vs. V4, d – V2 vs. V3, e – V2 vs. V4, f – V3 vs. V4.

From: Köhler H, Soschinka A, Meyer M, Kather A, Reinhold P, Liebler-Tenorio E. Characterization of a caprine model for the subclinical initial phase of Mycobacterium avium subsp. paratuberculosis infection. BMC Vet Res. 2015 Mar 24;11(1):74.

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  • Mouse anti Bovine Interferon Gamma
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    CC330
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2112E, ES, F *datasheet pdfdatasheet pdf0.5 mg
    MCA2112
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Bovine interferon γ antibody, clone CC330 recognizes bovine interferon gamma, a 143 amino acid ~20 kDa immunomodulatory cytokine secreted predominantly by T lymphocytes in response to infection by invading miro-organisms. Interferon γ enhances cytotoxic activities of many cell types including macrophages, NK cells and T lymphocytes (Weiss et al. 2002).
    • Intended Use
    • Target Species
      Bovine
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Sheepyes
      Goatyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      Recombinant bovine IFN-gamma.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      response to virus
      immune response
      interferon-gamma receptor binding
      extracellular space
      cytokine activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA5ug/ml as capture antibody
      ELISpot
      Flow Cytometry(1)1/501/100
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
    • ELISA
      This clone may be used in a sandwich ELISA in combination with clone CC302 (MCA1783B) and recombinant bovine IFN gamma (PBP007).
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IFN Gamma Antibody Formats

    Formats Clone Applications Sizes available
    IFN Gamma Antibody : Purified CC330 E, ES, F * 0.5 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      HCA036P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF
        MCA928

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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