IFN Gamma antibody | 7B6

Mouse anti Bovine Interferon Gamma

Product Type
Monoclonal Antibody
Clone
7B6
Isotype
IgG1
Specificity
IFN Gamma

Product Code Applications Pack Size List Price Your Price Qty
MCA1964
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F * P * WB 0.5 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Bovine interferon-γ antibody, clone 7B6 recognises both native and recombinant bovine IFN-γ. Bovine interferon-γ is a 166 amino acid ~18 kDa secreted cytokine with antiviral activity. Interferon-γ, secreted by lymphocytes in response to specific mitogens, is also a potent macrophage activator and has antiproliferative actions on transformed cells and can enhance functions of type-1 interferons.

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep
Fallow deer
Goat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1 1/500 1/2000
Immunohistology - Paraffin 2
Western Blotting
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
  2. 2This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 1x106 cells in 100μl

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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)

Description Product Code Applications Pack Size List Price Your Price Quantity
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Description Mouse IgG1 Negative Control

References for IFN Gamma antibody

  1. Weynants, V. et al. (1998) Quantitative assessment by flow cytometry of T-lymphocytes producing antigen-specific gamma-interferon in Brucella immune cattle.
    Vet Immunol Immunopathol. 66 (3-4): 309-20.
  2. Esteves, I. et al. (2004) Protective killed Ehrlichia ruminantium vaccine elicits IFN-gamma responses by CD4+ and CD8+ T lymphocytes in goats.
    Vet Immunol Immunopathol. 98 (1-2): 49-57.
  3. Gillan, S. et al. (2010) Identification of immune parameters to differentiate disease states among sheep infected with Mycobacterium avium subsp. paratuberculosis.
    Clin Vaccine Immunol. 17: 108-17.
  4. Stevens, E.T. et al. (2009) The induction of a cell-mediated immune response to bovine viral diarrhea virus with an adjuvanted inactivated vaccine.
    Vet Ther. 10: E1-8.
  5. Lei, L. et al. (2008) Live Mycobacterium avium subsp. paratuberculosis and a killed-bacterium vaccine induce distinct subcutaneous granulomas, with unique cellular and cytokine profiles.
    Clin Vaccine Immunol. 15: 783-93.
  6. Rogers, A.N. et al. (2005) Gammadelta T cell function varies with the expressed WC1 coreceptor.
    J Immunol. 174: 3386-93.
  7. Rosbottom, A. et al. (2008) Upregulation of cytokines is detected in the placentas of cattle infected with Neospora caninum and is more marked early in gestation when fetal death is observed.
    Infect Immun. 76 (6): 2352-61.
  8. García-Jiménez, W.L. (2012) Histological and immunohistochemical characterisation of Mycobacterium bovis induced granulomas in naturally infected fallow deer (Dama dama).
    Vet Immunol Immunopathol. 149: 66-75.
  9. View The Latest Product References
  10. Canal, A.M. et al. (2017) Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection.
    Res Vet Sci. 110: 34-39.
  11. Stevens, E.T. et al. (2009) The induction of a cell-mediated immune response to bovine viral diarrhea virus with an adjuvanted inactivated vaccine.
    Vet Ther. 10 (4): E1-8.
  12. Çomakli, S. & Özdemir, S. (2019) Comparative Evaluation of the Immune Responses in Cattle Mammary Tissues Naturally Infected with Bovine Parainfluenza Virus Type 3 and Bovine Alphaherpesvirus-1.
    Pathogens. 8 (1)Feb 25 [Epub ahead of print].
  13. Sirak, A. et al. (2021) Cellular and Cytokine Responses in Lymph Node Granulomas of Bacillus Calmette Guérin (BCG)-Vaccinated and Non-vaccinated Cross-Breed Calves Naturally Infected With Mycobacterium bovis.
    Front Vet Sci. 8: 698800.

Flow Cytometry

Immunohistology - Paraffin

Synonyms
Interferon Gamma
RRID
AB_2123455
UniProt
P07353
Entrez Gene
IFNG
GO Terms
GO:0009615 response to virus
GO:0005125 cytokine activity
GO:0005133 interferon-gamma receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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