IFN Gamma antibody | 7B6

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Anti Bovine Interferon Gamma Antibody, clone 7B6 thumbnail image 1

Anti Bovine Interferon Gamma Antibody, clone 7B6 thumbnail image 2

Anti Bovine Interferon Gamma Antibody, clone 7B6 gallery image 1 — **Published customer image:**
Mouse anti Bovine Interferon gamma antibody, clone 7B6 (**MCA1964**) used for the identification of gamma interferon immunoreactivity in bovine tissue sections by immunohistochemistry on formalin fixed, paraffin sections.
**Image caption:**
Immunohistochemical staining of IFN-γ in the Bovine mammary tissue using paraffin-embedded sections (A–C): (A) The intermediate immunoreactivity of IFN-γ (arrow) in the interalveolar connective tissue with the monoclonal antibody specific for IFN-γ in Bovine mammary tissue infected with BPIV-3. (B) The intermediate immunoreactivity of IFN-γ (arrow) in the interlobular connective tissue with the monoclonal antibody specific for IFN-γ in Bovine mammary tissue infected with BoHV-1. (C) The high immunoreactivity of IFN-γ (arrow) in the interalveolar connective tissue with the monoclonal antibody specific for IFN-γ in Bovine mammary tissue infected with BoHV-1 and BPIV-3. IHC. Bar, 20μm.

From: Çomakli S, & Özdemir S.
Comparative Evaluation of the Immune Responses in Cattle Mammary Tissues Naturally Infected with Bovine Parainfluenza Virus Type 3 and Bovine Alphaherpesvirus-1.
Pathogens. 2019;8(1). pii: E26.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.

Anti Bovine Interferon Gamma Antibody, clone 7B6 gallery image 2 — **Published customer image:**
Mouse anti Bovine interferon-γ antibody, clone 7B6 (**MCA1964**) used to assess interferon γ expression in bovine granulomatous lesions by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
**Image caption:**
Immunolabelling for IFN-γ⁺. (A) Comparison of IFN-γ⁺ cell distribution in different granuloma stages of BCG vaccinated (Vac) and non-vaccinated (Con) calves. (B) Stage I granuloma from non-vaccinated calf with some IFN-γ⁺ positively stained cells (arrows). (C) Stage I granuloma from BCG vaccinated calf has more lymphocytes stained for IFN-γ⁺ (arrows). (D) Stage IV granuloma showing IFN-γ⁺ stained cells from non-vaccinated calf (arrows). (E) Stage IV granuloma showing IFN-γ⁺ stained cells from BCG vaccinated calf (arrows). 50μm for (B,C), Bar equals 100μm in (D,E).

From: ISirak A, Tulu B, Bayissa B, Gumi B, Berg S, Salguero FJ, Ameni G; ETHICOBOTS Consortium.
Cellular and Cytokine Responses in Lymph Node Granulomas of Bacillus Calmette Guérin (BCG)-Vaccinated and Non-vaccinated Cross-Breed Calves Naturally Infected With *Mycobacterium bovis*.
Front Vet Sci. 2021 Sep 16;8:698800.
doi: 10.3389/fvets.2021.698800.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Mouse anti Bovine Interferon Gamma

Product Type: Monoclonal Antibody
Clone: 7B6
Isotype: IgG1

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
MCA1964 Datasheet	F* P* WB SDS	0.5 mg	Log in		Get Quote

Mouse anti Bovine interferon-γ antibody, clone 7B6 recognises both native and recombinant bovine IFN-γ. Bovine interferon-γ is a 166 amino acid ~18 kDa secreted cytokine with antiviral activity. Interferon-γ, secreted by lymphocytes in response to specific mitogens, is also a potent macrophage activator and has antiproliferative actions on transformed cells and can enhance functions of type-1 interferons.

Product Details

Target Species

Bovine

Species Cross-Reactivity

Target Species	Cross Reactivity
Sheep
Fallow deer
Goat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide

Carrier Free

Yes

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Storage Information

Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee: 12 months from date of despatch

More Information

UniProt: P07353
Entrez Gene: IFNG
GO Terms: GO:0009615 response to virus; GO:0005125 cytokine activity; GO:0005133 interferon-gamma receptor binding; GO:0005615 extracellular space; GO:0006955 immune response
Regulatory: For research purposes only

Applications of IFN Gamma antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA
Flow Cytometry ¹	1/500	1/2000
Immunohistology - Paraffin ²
Western Blotting

¹Membrane permeabilization is required for this application. The use of Leucoperm^™ (Product Code BUF09A) is recommended for this purpose.
²This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	E WB	0.5 mg	Log in	Get Quote
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	F IF	0.1 mg	Log in	Get Quote
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)	STAR117D550	F IF WB	0.1 mg	Log in	Get Quote
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)	STAR117D650	F IF	0.1 mg	Log in	Get Quote
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	F WB	0.1 mg	Log in	Get Quote
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	F IF WB	0.1 mg	Log in	Get Quote
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	F	0.5 mg	Log in	Get Quote
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	C E WB	0.5 mg	Log in	Get Quote
Goat anti Mouse IgG (Fc):FITC	STAR120F	C F	1 mg	Log in	Get Quote
Goat anti Mouse IgG (Fc):HRP	STAR120P	E WB	1 mg	Log in	Get Quote
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	F	1 ml	Log in	Get Quote
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	C E P RE WB	1 mg	Log in	Get Quote
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	F	0.5 mg	Log in	Get Quote
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	F	1 ml	Log in	Get Quote
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	C E P	0.5 mg	Log in	Get Quote
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	C E WB	1 mg	Log in	Get Quote
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	E	1 mg	Log in	Get Quote
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	F IF WB	0.1 mg	Log in	Get Quote
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	F	1 mg	Log in	Get Quote

Negative Isotype Controls Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Mouse IgG1 Negative Control	MCA928	F	100 Tests	Log in		Get Quote

Application Based External Images

Flow Cytometry

Immunohistology - Paraffin

Product Specific References

References for IFN Gamma antibody

Weynants, V. et al. (1998) Quantitative assessment by flow cytometry of T-lymphocytes producing antigen-specific gamma-interferon in Brucella immune cattle.
Vet Immunol Immunopathol. 66 (3-4): 309-20.
Esteves, I. et al. (2004) Protective killed Ehrlichia ruminantium vaccine elicits IFN-gamma responses by CD4+ and CD8+ T lymphocytes in goats.
Vet Immunol Immunopathol. 98 (1-2): 49-57.
Gillan, S. et al. (2010) Identification of immune parameters to differentiate disease states among sheep infected with Mycobacterium avium subsp. paratuberculosis.
Clin Vaccine Immunol. 17: 108-17.
Stevens, E.T. et al. (2009) The induction of a cell-mediated immune response to bovine viral diarrhea virus with an adjuvanted inactivated vaccine.
Vet Ther. 10: E1-8.
Lei, L. et al. (2008) Live Mycobacterium avium subsp. paratuberculosis and a killed-bacterium vaccine induce distinct subcutaneous granulomas, with unique cellular and cytokine profiles.
Clin Vaccine Immunol. 15: 783-93.
Rogers, A.N. et al. (2005) Gammadelta T cell function varies with the expressed WC1 coreceptor.
J Immunol. 174: 3386-93.
Rosbottom, A. et al. (2008) Upregulation of cytokines is detected in the placentas of cattle infected with Neospora caninum and is more marked early in gestation when fetal death is observed.
Infect Immun. 76 (6): 2352-61.
García-Jiménez, W.L. (2012) Histological and immunohistochemical characterisation of Mycobacterium bovis induced granulomas in naturally infected fallow deer (Dama dama).
Vet Immunol Immunopathol. 149: 66-75.
Canal, A.M. et al. (2017) Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection.
Res Vet Sci. 110: 34-39.
Stevens, E.T. et al. (2009) The induction of a cell-mediated immune response to bovine viral diarrhea virus with an adjuvanted inactivated vaccine.
Vet Ther. 10 (4): E1-8.
Çomakli, S. & Özdemir, S. (2019) Comparative Evaluation of the Immune Responses in Cattle Mammary Tissues Naturally Infected with Bovine Parainfluenza Virus Type 3 and Bovine Alphaherpesvirus-1.
Pathogens. 8 (1)Feb 25 [Epub ahead of print].
Sirak, A. et al. (2021) Cellular and Cytokine Responses in Lymph Node Granulomas of Bacillus Calmette Guérin (BCG)-Vaccinated and Non-vaccinated Cross-Breed Calves Naturally Infected With Mycobacterium bovis.
Front Vet Sci. 8: 698800.

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