IFN Gamma antibody | 7B6

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Anti Bovine Interferon Gamma Antibody, clone 7B6 gallery image 1
Published customer image:
Mouse anti Bovine Interferon gamma antibody, clone 7B6 (MCA1964) used for the identification of gamma interferon immunoreactivity in bovine tissue sections by immunohistochemistry on formalin fixed, paraffin sections.
Image caption:
Immunohistochemical staining of IFN-γ in the Bovine mammary tissue using paraffin-embedded sections (A–C): (A) The intermediate immunoreactivity of IFN-γ (arrow) in the interalveolar connective tissue with the monoclonal antibody specific for IFN-γ in Bovine mammary tissue infected with BPIV-3. (B) The intermediate immunoreactivity of IFN-γ (arrow) in the interlobular connective tissue with the monoclonal antibody specific for IFN-γ in Bovine mammary tissue infected with BoHV-1. (C) The high immunoreactivity of IFN-γ (arrow) in the interalveolar connective tissue with the monoclonal antibody specific for IFN-γ in Bovine mammary tissue infected with BoHV-1 and BPIV-3. IHC. Bar, 20μm.

From: Çomakli S, Özdemir S. Comparative Evaluation of the Immune Responses in Cattle Mammary Tissues Naturally Infected with Bovine Parainfluenza Virus Type 3 and Bovine Alphaherpesvirus-1. Pathogens. 2019;8(1). pii: E26.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Bovine Interferon Gamma

Product Type
Monoclonal Antibody
Clone
7B6
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA1964
Datasheet
F* P* WB
MSDS
0.5 mg loader

Mouse anti Bovine interferon-γ antibody, clone 7B6 recognises both native and recombinant bovine IFN-γ. Bovine interferon-γ is a 166 amino acid ~18 kDa secreted cytokine with antiviral activity. Interferon-γ, secreted by lymphocytes in response to specific mitogens, is also a potent macrophage activator and has antiproliferative actions on transformed cells and can enhance functions of type-1 interferons.

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep
Fallow deer
Goat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
P07353 Related reagents
Entrez Gene
IFNG Related reagents
GO Terms
GO:0009615 response to virus
GO:0005125 cytokine activity
GO:0005133 interferon-gamma receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
Regulatory
For research purposes only

Applications of IFN Gamma antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1 1/500 1/2000
Immunohistology - Paraffin 2
Western Blotting
  1. 1Membrane permeabilization is required for this application. The use of Leucoperm™ (Product Code BUF09A) is recommended for this purpose.
  2. 2This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader

Application Based External Images

ELISA

Flow Cytometry

Immunohistology - Paraffin

Product Specific References

References for IFN Gamma antibody

  1. Weynants, V. et al. (1998) Quantitative assessment by flow cytometry of T-lymphocytes producing antigen-specific gamma-interferon in Brucella immune cattle.
    Vet Immunol Immunopathol. 66 (3-4): 309-20.
  2. Esteves, I. et al. (2004) Protective killed Ehrlichia ruminantium vaccine elicits IFN-gamma responses by CD4+ and CD8+ T lymphocytes in goats.
    Vet Immunol Immunopathol. 98 (1-2): 49-57.
  3. Gillan, S. et al. (2010) Identification of immune parameters to differentiate disease states among sheep infected with Mycobacterium avium subsp. paratuberculosis.
    Clin Vaccine Immunol. 17: 108-17.
  4. Stevens, E.T. et al. (2009) The induction of a cell-mediated immune response to bovine viral diarrhea virus with an adjuvanted inactivated vaccine.
    Vet Ther. 10: E1-8.
  5. Lei, L. et al. (2008) Live Mycobacterium avium subsp. paratuberculosis and a killed-bacterium vaccine induce distinct subcutaneous granulomas, with unique cellular and cytokine profiles.
    Clin Vaccine Immunol. 15: 783-93.
  6. Rogers, A.N. et al. (2005) Gammadelta T cell function varies with the expressed WC1 coreceptor.
    J Immunol. 174: 3386-93.
  7. Rosbottom, A. et al. (2008) Upregulation of cytokines is detected in the placentas of cattle infected with Neospora caninum and is more marked early in gestation when fetal death is observed.
    Infect Immun. 76 (6): 2352-61.
  8. García-Jiménez, W.L. (2012) Histological and immunohistochemical characterisation of Mycobacterium bovis induced granulomas in naturally infected fallow deer (Dama dama).
    Vet Immunol Immunopathol. 149: 66-75.
  9. Canal, A.M. et al. (2017) Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection.
    Res Vet Sci. 110: 34-39.
  10. Stevens, E.T. et al. (2009) The induction of a cell-mediated immune response to bovine viral diarrhea virus with an adjuvanted inactivated vaccine.
    Vet Ther. 10 (4): E1-8.
  11. Çomakli, S. & Özdemir, S. (2019) Comparative Evaluation of the Immune Responses in Cattle Mammary Tissues Naturally Infected with Bovine Parainfluenza Virus Type 3 and Bovine Alphaherpesvirus-1.
    Pathogens. 8 (1)Feb 25 [Epub ahead of print].
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