CD5 antibody | CC17

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Anti Bovine CD5 Antibody, clone CC17 thumbnail image 1

Anti Bovine CD5 Antibody, clone CC17 thumbnail image 2

Anti Bovine CD5 Antibody, clone CC17 thumbnail image 3

Anti Bovine CD5 Antibody, clone CC17 thumbnail image 4

Anti Bovine CD5 Antibody, clone CC17 gallery image 1 — Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD5 (**MCA835G**).

Anti Bovine CD5 Antibody, clone CC17 gallery image 2 — Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD5 (**MCA835G**) followed by Goat anti Mouse IgG (H/L):FITC (STAR117F).

Anti Bovine CD5 Antibody, clone CC17 gallery image 3 — Figure A. Purified mouse anti bovine CD5 (**MCA835GA**) detected with goat anti mouse IgG1 RPE (STAR132PE) and FITC conjugated mouse IgG2a isotype control (MCA929F). Figure B. Purified mouse anti bovine CD5 (**MCA835GA**) detected with goat anti mouse IgG1 RPE (STAR132PE) and FITC conjugated mouse anti bovine CD4 (MCA1653F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

Anti Bovine CD5 Antibody, clone CC17 gallery image 4 — Figure A. FITC conjugated mouse anti bovine CD4 (MCA1653F) and purified mouse IgG1 isotype control (MCA928) detected with RPE conjugated goat anti mouse IgG1 (STAR132PE). Figure B. FITC conjugated mouse anti bovine CD4 (MCA1653F) and purified CD5 (**MCA835GA**) detected with RPE conjugated goat anti mouse IgG1 (STAR132PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

Mouse anti Bovine CD5

Product Type: Monoclonal Antibody
Clone: CC17
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA835GA	C F IP			0.1 mg

Mouse anti Bovine CD5 antibody, clone CC15 recognizes bovine CD5, a ~67 kDa type 1 single pass transmembrane molecule containing three scavenger receptor cysteine rich (SRCR) domains. Clone CC17 reacts with BoCD5.1, which is a polymorphic antigen expressed on cells of all Bos taurus animals and a small proportion of Bos indicus animals.
Bo5.1, recognized by Mouse anti Bovine CD5, clone CC17 is the only isoform expressed by Bos taurus while Bos indicus may express Bo5.2 or both allelic forms of bovine CD5. (Howard et al.1989) .

Bovine CD5 is expressed by all mature T-lymphocytes and a subpopulation of B-lymphocytes. It is also expressed by mature medullary thymocytes and at a lower level by immature cortical thymocytes (Howard et al. 1988).

Product Details

Target Species

Bovine

Species Cross-Reactivity

Target Species	Cross Reactivity
Goat
Sheep

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Carrier Free

Yes

Immunogen

Bovine thymocytes

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells from an immunised mouse were fused with cells of the mouse NS1 myeloma cell line

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch

More Information

UniProt: P19238 Related reagents
Entrez Gene: CD5 Related reagents
GO Terms: GO:0016021 integral to membrane; GO:0005044 scavenger receptor activity
Regulatory: For research purposes only

Applications of CD5 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry	1/25	1/100
Immunohistology - Frozen
Immunoprecipitation

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG1 Negative Control	MCA928	100 Tests	F

Application Based External Images

Flow Cytometry

Product Specific References

References for CD5 antibody

Howard, C.J. & Leibold, W. (1991) Individual antigens of cattle. Bovine CD5 (BoCD5).
Vet Immunol Immunopathol. 27(1-3): 55-60.
Howard, C.J. et al. (1988) Two monoclonal antibodies (CC17, CC29) recognizing an antigen (Bo5) on bovine T lymphocytes, analogous to human CD5.
Vet Immunol Immunopathol. 19(2): 127-39.
Howard, C.J. et al. (1991) Competitive binding with putative Bo5 (CD5) cluster of monoclonal antibodies.
Vet Immunol Immunopathol. 27(1-3): 147-51.
Hein, W.R. et al. (1991) Comparison of reactivity of monoclonal antibodies on bovine, ovine and caprine tissues and on cells from other animal species.
Vet Immunol Immunopathol. 27(1-3): 32-4.
Elhmouzi-Younes, J. et al. (2010) Ovine CD16+/CD14- blood lymphocytes present all the major characteristics of natural killer cells.
Vet Res. 41(1): 4.
Haas, K.M. & Estes, D.M. (2000) Activation of bovine B cells via surface immunoglobulin M cross-linking or CD40 ligation results in different B-cell phenotypes.
Immunology 99: 272-8.
Nfon, C.K. et al. (2012) Innate immune response to Rift Valley fever virus in goats.
PLoS Negl Trop Dis. 6: e1623.

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