CD5 antibody | CC17

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Mouse anti Bovine CD5

Product Type
Monoclonal Antibody
Clone
CC17
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA835GA C F IP 0.1 mg
Mouse anti Bovine CD5 antibody, clone CC15 recognizes bovine CD5, a ~67 kDa type 1 single pass transmembrane molecule containing three scavenger receptor cysteine rich (SRCR) domains. Clone CC17 reacts with BoCD5.1, which is a polymorphic antigen expressed on cells of all Bos taurus animals and a small proportion of Bos indicus animals.
Bo5.1, recognized by Mouse anti Bovine CD5, clone CC17 is the only isoform expressed by Bos taurus while Bos indicus may express Bo5.2 or both allelic forms of bovine CD5. (Howard et al.1989) .

Bovine CD5 is expressed by all mature T-lymphocytes and a subpopulation of B-lymphocytes. It is also expressed by mature medullary thymocytes and at a lower level by immature cortical thymocytes (Howard et al. 1988).

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Goat
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Bovine thymocytes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from an immunised mouse were fused with cells of the mouse NS1 myeloma cell line

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch

More Information

UniProt
P19238 Related reagents
Entrez Gene
CD5 Related reagents
GO Terms
GO:0016021 integral to membrane
GO:0005044 scavenger receptor activity
Regulatory
For research purposes only

Applications of CD5 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/100
Immunohistology - Frozen
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F

Application Based External Images

Flow Cytometry

Product Specific References

References for CD5 antibody

  1. Howard, C.J. & Leibold, W. (1991) Individual antigens of cattle. Bovine CD5 (BoCD5).
    Vet Immunol Immunopathol. 27(1-3): 55-60.
  2. Howard, C.J. et al. (1988) Two monoclonal antibodies (CC17, CC29) recognizing an antigen (Bo5) on bovine T lymphocytes, analogous to human CD5.
    Vet Immunol Immunopathol. 19(2): 127-39.
  3. Howard, C.J. et al. (1991) Competitive binding with putative Bo5 (CD5) cluster of monoclonal antibodies.
    Vet Immunol Immunopathol. 27(1-3): 147-51.
  4. Hein, W.R. et al. (1991) Comparison of reactivity of monoclonal antibodies on bovine, ovine and caprine tissues and on cells from other animal species.
    Vet Immunol Immunopathol. 27(1-3): 32-4.
  5. Elhmouzi-Younes, J. et al. (2010) Ovine CD16+/CD14- blood lymphocytes present all the major characteristics of natural killer cells.
    Vet Res. 41(1): 4.
  6. Haas, K.M. & Estes, D.M. (2000) Activation of bovine B cells via surface immunoglobulin M cross-linking or CD40 ligation results in different B-cell phenotypes.
    Immunology 99: 272-8.
  7. Nfon, C.K. et al. (2012) Innate immune response to Rift Valley fever virus in goats.
    PLoS Negl Trop Dis. 6: e1623.