CD45RB antibody | CC76

Mouse anti Bovine CD45RB

Product Type
Monoclonal Antibody
Clone
CC76
Isotype
IgG1
Specificity
CD45RB

Product Code Applications Pack Size List Price Your Price Qty
MCA1650GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Bovine CD45RB, clone CC76, recognizes the RB isoform of the bovine leucocyte common antigen expressed on subpopulations of CD4+ and CD8+ T cells. Expression levels of CD45RB are used as a marker for differentiating naïve and memory T lymphocytes (Howard et al. 1991). The antigen recognized by clone CC76 is also expressed on B cells but is absent from both macrophages and granulocytes.

Target Species
Bovine
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Bovine thymocytes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c micewere fused with cells of the NS1 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/100 1/200
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

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References for CD45RB antibody

  1. Howard, C.J. et al. (1991) Distinction of naive and memory BoCD4 lymphocytes in calves with a monoclonal antibody, CC76, to a restricted determinant of the bovine leukocyte-common antigen, CD45.
    Eur J Immunol. 21 (9): 2219-26.
  2. Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle (tables 1 and 2).
    Vet Immunol Immunopathol. 39 (1-3): 25-47.
  3. Sopp, P. & Howard, C.J. (2001) IFN gamma and IL-4 production by CD4, CD8 and WC1 gamma delta TCR(+) T cells from cattle lymph nodes and blood.
    Vet Immunol Immunopathol. 81 (1-2): 85-96.
  4. Bembridge, G.P. et al. (1993) Comparison of monoclonal antibodies with potential specificity for restricted isoforms of the leukocyte common antigen (CD45R).
    Vet Immunol Immunopathol. 39 (1-3): 129-36.
  5. Reid, E. et al. (2011) Bovine plasmacytoid dendritic cells are the major source of type I interferon in response to foot-and-mouth disease virus in vitro and in vivo.
    J Virol. 85: 4297-308.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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