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CD1w2 antibody | CC20

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Anti Bovine CD1w2 Antibody, clone CC20 thumbnail image 1 Anti Bovine CD1w2 Antibody, clone CC20 thumbnail image 2 Anti Bovine CD1w2 Antibody, clone CC20 thumbnail image 3 Anti Bovine CD1w2 Antibody, clone CC20 thumbnail image 4 Anti Bovine CD1w2 Antibody, clone CC20 thumbnail image 5
Anti Bovine CD1w2 Antibody, clone CC20 gallery image 1
Figure A. FITC conjugated Mouse anti Bovine MHC Class II Monomorphic Antibody (MCA2445F) and RPE conjugated Mouse IgG2a isotype control (MCA929PE). Figure B. FITC conjugated Mouse anti Bovine MHC Class II Monomorphic Antibody (MCA2445F) and RPE conjugated Mouse anti Bovine CD1w2 Antibody (MCA2058PE). All experiments performed on bovine thymocytes in the presence of 10% bovine serum and gated on live, single cell thymocytes. Data acquired on the ZE5™ Cell Analyzer.
Anti Bovine CD1w2 Antibody, clone CC20 gallery image 2
Figure A. FITC conjugated Mouse anti Bovine CD4 Antibody (MCA1653F) and Mouse IgG2a isotype control (MCA929) conjugated with Rabbit F(ab')2 anti Mouse IgG:RPE Antibody (STAR12A). Figure B. FITC conjugated Mouse anti Bovine CD4 Antibody (MCA1653F) and Mouse anti Bovine CD1w2 Antibody (MCA2058GA) conjugated with Rabbit F(ab')2 anti Mouse IgG:RPE Antibody (STAR12A). All experiments performed on bovine thymocytes in the presence of 10% bovine serum and gated on live, single cell thymocytes. Data acquired on the ZE5™ Cell Analyzer.
Anti Bovine CD1w2 Antibody, clone CC20 gallery image 3
Figure A. FITC conjugated Mouse anti Bovine CD4 Antibody (MCA1653F) and RPE conjugated Mouse IgG2a isotype control (MCA929PE). Figure B. FITC conjugated Mouse anti Bovine CD4 Antibody (MCA1653F) and RPE conjugated Mouse anti Bovine CD1w2 Antibody (MCA2058PE). All experiments performed on bovine thymocytes in the presence of 10% bovine serum and gated on live, single cell thymocytes. Data acquired on the ZE5™ Cell Analyzer.
Anti Bovine CD1w2 Antibody, clone CC20 gallery image 4
Figure A. FITC conjugated Mouse anti Bovine MHC Class II Monomorphic Antibody (MCA2445F) and Mouse IgG2a isotype control (MCA929) conjugated with Rabbit F(ab')2 anti Mouse IgG:RPE Antibody (STAR12A). Figure B. FITC conjugated Mouse anti Bovine MHC Class II Monomorphic Antibody (MCA2445F) and Mouse anti Bovine CD1w2 Antibody (MCA2058GA) conjugated with Rabbit F(ab')2 anti Mouse IgG:RPE Antibody (STAR12A). All experiments performed on bovine thymocytes in the presence of 10% bovine serum and gated on live, single cell thymocytes. Data acquired on the ZE5™ Cell Analyzer.
Anti Bovine CD1w2 Antibody, clone CC20 gallery image 5
Detection of Mouse anti Bovine CD1w2 (MCA2058GA) in an immunohistochemical analysis on acetone-fixed frozen bovine thymus. Original magnification x60.

Mouse anti Bovine CD1w2:Alexa Fluor® 647

Mouse anti Bovine CD1w2

Mouse anti Bovine CD1w2:RPE

Product Type
Monoclonal Antibody
Clone
CC20
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA2058A647
Datasheet
F
SDS
100 Tests/1ml loader

MCA2058G
Datasheet
C F IP
SDS
0.25 mg loader

MCA2058PE
Datasheet
F
SDS
100 Tests loader

Mouse anti Bovine CD1w2 antibody, clone CC20 recognises the bovine CD1w2 cell surface antigen, a glycoprotein heterodimer of ~12 kDa and ~46 kDa. CD1w2 is expressed by dendritic cells, cortical thymocytes and a minority of medullary thymocytes, with a pattern similar to antibodies of the CD1b cluster in humans.

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep
Goat
Dog
Horse
Cat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor 647 - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Carrier Free
Yes
Approx. Protein Concentrations
Ig concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised mice were fused with cells of the mouse NS1 myeloma cell line.

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD1w2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry 1/25 1/50
Immunohistology - Frozen 1/1000
Immunoprecipitation
Flow Cytometry Neat 1/5
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgG2a:HRP HCA037P E 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG2a Negative Control:Alexa Fluor® 647 MCA929A647 F 100 Tests/1ml loader
Mouse IgG2a Negative Control MCA929 F 100 Tests loader
Mouse IgG2a Negative Control:RPE MCA929PE F 100 Tests loader

Application Based External Images

Flow Cytometry

Product Specific References

References for CD1w2 antibody

  1. Siedek, E. et al. (1997) Isolation and characterisation of equine dendritic cells.
    Vet Immunol Immunopathol. 60 (1-2): 15-31.
  2. Hein, W.R. et al. (1991) Summary of workshop findings for leukocyte antigens of sheep.
    Vet Immunol Immunopathol. 27 (1-3): 28-30.
  3. Romero-Palomo, F. et al. (2015) Immunopathologic Changes in the Thymus of Calves Pre-infected with BVDV and Challenged with BHV-1.
    Transbound Emerg Dis. Aug 25. [Epub ahead of print]
  4. Howard, C.J. et al. (1993) Comparison of CD1 monoclonal antibodies on bovine cells and tissues.
    Vet Immunol Immunopathol. 39 (1-3): 77-83.
  5. Rhind, S.M. (2001) CD1--the pathology perspective.
    Vet Pathol. 38 (6): 611-9.
  6. Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle (tables 1 and 2).
    Vet Immunol Immunopathol. 39 (1-3): 25-47.
  7. Åkesson, C.P. et al. (2008) Phenotypic characterisation of intestinal dendritic cells in sheep.
    Dev Comp Immunol. 32: 837-49.
  8. Chan, S.S. et al. (2002) Generation and characterization of ovine dendritic cells derived from peripheral blood monocytes.
    Immunology. 107: 366-72.
  9. Shu, D. et al. (2009) Cutaneous cytokine gene expression and cellular responses in lambs infested with the louse, Bovicola ovis, and following intradermal injection of crude louse antigen.
    Vet Immunol Immunopathol. 129: 82-92.
  10. McNeilly, T.N. et al. (2006) Differential expression of cell surface markers by ovine respiratory tract dendritic cells.
    J Histochem Cytochem. 54: 1021-30.
  11. Mérant, C. et al. (2009) Young foal and adult horse monocyte-derived dendritic cells differ by their degree of phenotypic maturity.
    Vet Immunol Immunopathol. 131: 1-8.
  12. Affolter, V.K. and Moore, P.F. (2002) Localized and disseminated histiocytic sarcoma of dendritic cell origin in dogs.
    Vet Pathol. 39: 74-83.
  13. Moore, P.F. et al. (1996) Canine cutaneous histiocytoma is an epidermotropic Langerhans cell histiocytosis that expresses CD1 and specific beta 2-integrin molecules.
    Am J Pathol. 148: 1699-708.
  14. Bienzle, D. et al. (2003) Immunophenotype and functional properties of feline dendritic cells derived from blood and bone marrow.
    Vet Immunol Immunopathol. 96: 19-30.
  15. Romero-palomo, F. et al. (2013) Immunohistochemical detection of dendritic cell markers in cattle.
    Vet Pathol. 50 (6): 1099-108.

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