CD1w2 antibody | CC20
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F C ResetMouse anti Bovine CD1w2
- Product Type
- Monoclonal Antibody
- Clone
- CC20
- Isotype
- IgG2a
- Specificity
- CD1w2
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Antibody Quality and 
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| Mouse anti Bovine CD1w2 antibody, clone CC20 recognises the bovine CD1w2 cell surface antigen, a glycoprotein heterodimer of ~12 kDa and ~46 kDa. CD1w2 is expressed by dendritic cells, cortical thymocytes and a minority of medullary thymocytes, with a pattern similar to antibodies of the CD1b cluster in humans. |
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Sheep Goat Dog Horse Cat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the mouse NS1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/50 |
| Immunohistology - Frozen | |
1/1000 | |
| Immunoprecipitation | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG2a Negative Control | ||||||
References for CD1w2 antibody
-
Siedek, E. et al. (1997) Isolation and characterisation of equine dendritic cells.
Vet Immunol Immunopathol. 60 (1-2): 15-31. -
Hein, W.R. et al. (1991) Summary of workshop findings for leukocyte antigens of sheep.
Vet Immunol Immunopathol. 27 (1-3): 28-30. -
Romero-Palomo, F. et al. (2017) Immunopathologic Changes in the Thymus of Calves Pre-infected with BVDV and Challenged with BHV-1.
Transbound Emerg Dis. 64 (2): 574-84. -
Howard, C.J. et al. (1993) Comparison of CD1 monoclonal antibodies on bovine cells and tissues.
Vet Immunol Immunopathol. 39 (1-3): 77-83. -
Rhind, S.M. (2001) CD1--the pathology perspective.
Vet Pathol. 38 (6): 611-9. -
Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle (tables 1 and 2).
Vet Immunol Immunopathol. 39 (1-3): 25-47. -
Åkesson, C.P. et al. (2008) Phenotypic characterisation of intestinal dendritic cells in sheep.
Dev Comp Immunol. 32: 837-49. -
Chan, S.S. et al. (2002) Generation and characterization of ovine dendritic cells derived from peripheral blood monocytes.
Immunology. 107: 366-72.
View The Latest Product References
-
Shu, D. et al. (2009) Cutaneous cytokine gene expression and cellular responses in lambs infested with the louse, Bovicola ovis, and following intradermal injection of crude louse antigen.
Vet Immunol Immunopathol. 129: 82-92. -
McNeilly, T.N. et al. (2006) Differential expression of cell surface markers by ovine respiratory tract dendritic cells.
J Histochem Cytochem. 54: 1021-30. -
Mérant, C. et al. (2009) Young foal and adult horse monocyte-derived dendritic cells differ by their degree of phenotypic maturity.
Vet Immunol Immunopathol. 131: 1-8. -
Affolter, V.K. and Moore, P.F. (2002) Localized and disseminated histiocytic sarcoma of dendritic cell origin in dogs.
Vet Pathol. 39: 74-83. -
Moore, P.F. et al. (1996) Canine cutaneous histiocytoma is an epidermotropic Langerhans cell histiocytosis that expresses CD1 and specific beta 2-integrin molecules.
Am J Pathol. 148: 1699-708. -
Bienzle, D. et al. (2003) Immunophenotype and functional properties of feline dendritic cells derived from blood and bone marrow.
Vet Immunol Immunopathol. 96: 19-30. -
Romero-palomo, F. et al. (2013) Immunohistochemical detection of dendritic cell markers in cattle.
Vet Pathol. 50 (6): 1099-108.
- Synonyms
- CD1b
- RRID
- AB_566607
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