CD1w2 antibody | CC20

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Mouse anti Bovine CD1w2

Mouse anti Bovine CD1w2:RPE

Product Type
Monoclonal Antibody
Clone
CC20
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2058G C F IP 0.25 mg
MCA2058PE F 100 Tests
Mouse anti Bovine CD1w2 antibody, clone CC20 recognises the bovine CD1w2 cell surface antigen, a glycoprotein heterodimer of ~12 kDa and ~46 kDa. CD1w2 is expressed by dendritic cells, cortical thymocytes and a minority of medullary thymocytes, with a pattern similar to antibodies of the CD1b cluster in humans.

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep
Goat
Dog
Horse
Cat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised mice were fused with cells of the mouse NS1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of reconstitution.

More Information

Regulatory
For research purposes only

Applications of CD1w2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/50
Immunohistology - Frozen 1/1000
Immunoprecipitation
Flow Cytometry Neat 1/5
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG2a Negative Control MCA929 100 Tests F
Mouse IgG2a Negative Control:RPE MCA929PE 100 Tests F

Application Based External Images

Flow Cytometry

Product Specific References

References for CD1w2 antibody

  1. Siedek, E. et al. (1997) Isolation and characterisation of equine dendritic cells.
    Vet Immunol Immunopathol. 60 (1-2): 15-31.
  2. Hein, W.R. et al. (1991) Summary of workshop findings for leukocyte antigens of sheep.
    Vet Immunol Immunopathol. 27 (1-3): 28-30.
  3. Romero-Palomo, F. et al. (2015) Immunopathologic Changes in the Thymus of Calves Pre-infected with BVDV and Challenged with BHV-1.
    Transbound Emerg Dis. Aug 25. [Epub ahead of print]
  4. Howard, C.J. et al. (1993) Comparison of CD1 monoclonal antibodies on bovine cells and tissues.
    Vet Immunol Immunopathol. 39 (1-3): 77-83.
  5. Rhind, S.M. (2001) CD1--the pathology perspective.
    Vet Pathol. 38 (6): 611-9.
  6. Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle (tables 1 and 2).
    Vet Immunol Immunopathol. 39 (1-3): 25-47.
  7. Åkesson, C.P. et al. (2008) Phenotypic characterisation of intestinal dendritic cells in sheep.
    Dev Comp Immunol. 32: 837-49.
  8. Chan, S.S. et al. (2002) Generation and characterization of ovine dendritic cells derived from peripheral blood monocytes.
    Immunology. 107: 366-72.
  9. Shu, D. et al. (2009) Cutaneous cytokine gene expression and cellular responses in lambs infested with the louse, Bovicola ovis, and following intradermal injection of crude louse antigen.
    Vet Immunol Immunopathol. 129: 82-92.
  10. McNeilly, T.N. et al. (2006) Differential expression of cell surface markers by ovine respiratory tract dendritic cells.
    J Histochem Cytochem. 54: 1021-30.
  11. Mérant, C. et al. (2009) Young foal and adult horse monocyte-derived dendritic cells differ by their degree of phenotypic maturity.
    Vet Immunol Immunopathol. 131: 1-8.
  12. Affolter, V.K. and Moore, P.F. (2002) Localized and disseminated histiocytic sarcoma of dendritic cell origin in dogs.
    Vet Pathol. 39: 74-83.
  13. Moore, P.F. et al. (1996) Canine cutaneous histiocytoma is an epidermotropic Langerhans cell histiocytosis that expresses CD1 and specific beta 2-integrin molecules.
    Am J Pathol. 148: 1699-708.
  14. Bienzle, D. et al. (2003) Immunophenotype and functional properties of feline dendritic cells derived from blood and bone marrow.
    Vet Immunol Immunopathol. 96: 19-30.
  15. Romero-palomo, F. et al. (2013) Immunohistochemical detection of dendritic cell markers in cattle.
    Vet Pathol. 50 (6): 1099-108.