CD1w2 antibody | CC20

Mouse anti Bovine CD1w2

Product Type
Monoclonal Antibody
Clone
CC20
Isotype
IgG2a
Specificity
CD1w2

Product Code Applications Pack Size List Price Your Price Qty
MCA2058G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 0.25 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Bovine CD1w2 antibody, clone CC20 recognises the bovine CD1w2 cell surface antigen, a glycoprotein heterodimer of ~12 kDa and ~46 kDa. CD1w2 is expressed by dendritic cells, cortical thymocytes and a minority of medullary thymocytes, with a pattern similar to antibodies of the CD1b cluster in humans.

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep
Goat
Dog
Horse
Cat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised mice were fused with cells of the mouse NS1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/50
Immunohistology - Frozen 1/1000
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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References for CD1w2 antibody

  1. Siedek, E. et al. (1997) Isolation and characterisation of equine dendritic cells.
    Vet Immunol Immunopathol. 60 (1-2): 15-31.
  2. Hein, W.R. et al. (1991) Summary of workshop findings for leukocyte antigens of sheep.
    Vet Immunol Immunopathol. 27 (1-3): 28-30.
  3. Romero-Palomo, F. et al. (2017) Immunopathologic Changes in the Thymus of Calves Pre-infected with BVDV and Challenged with BHV-1.
    Transbound Emerg Dis. 64 (2): 574-84.
  4. Howard, C.J. et al. (1993) Comparison of CD1 monoclonal antibodies on bovine cells and tissues.
    Vet Immunol Immunopathol. 39 (1-3): 77-83.
  5. Rhind, S.M. (2001) CD1--the pathology perspective.
    Vet Pathol. 38 (6): 611-9.
  6. Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle (tables 1 and 2).
    Vet Immunol Immunopathol. 39 (1-3): 25-47.
  7. Åkesson, C.P. et al. (2008) Phenotypic characterisation of intestinal dendritic cells in sheep.
    Dev Comp Immunol. 32: 837-49.
  8. Chan, S.S. et al. (2002) Generation and characterization of ovine dendritic cells derived from peripheral blood monocytes.
    Immunology. 107: 366-72.
  9. View The Latest Product References
  10. Shu, D. et al. (2009) Cutaneous cytokine gene expression and cellular responses in lambs infested with the louse, Bovicola ovis, and following intradermal injection of crude louse antigen.
    Vet Immunol Immunopathol. 129: 82-92.
  11. McNeilly, T.N. et al. (2006) Differential expression of cell surface markers by ovine respiratory tract dendritic cells.
    J Histochem Cytochem. 54: 1021-30.
  12. Mérant, C. et al. (2009) Young foal and adult horse monocyte-derived dendritic cells differ by their degree of phenotypic maturity.
    Vet Immunol Immunopathol. 131: 1-8.
  13. Affolter, V.K. and Moore, P.F. (2002) Localized and disseminated histiocytic sarcoma of dendritic cell origin in dogs.
    Vet Pathol. 39: 74-83.
  14. Moore, P.F. et al. (1996) Canine cutaneous histiocytoma is an epidermotropic Langerhans cell histiocytosis that expresses CD1 and specific beta 2-integrin molecules.
    Am J Pathol. 148: 1699-708.
  15. Bienzle, D. et al. (2003) Immunophenotype and functional properties of feline dendritic cells derived from blood and bone marrow.
    Vet Immunol Immunopathol. 96: 19-30.
  16. Romero-palomo, F. et al. (2013) Immunohistochemical detection of dendritic cell markers in cattle.
    Vet Pathol. 50 (6): 1099-108.

Flow Cytometry

Synonyms
CD1b
RRID
AB_566607
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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