CD172a antibody | CC149
Mouse anti Bovine CD172a antibody, clone CC149 (MCA2041GA) used for the evaluation of CD172a expression on ovine bone marrow aspirate cells by flow cytometry.
Image caption:
BM of Csf1r-EGFP sheep contains a heterogeneous EGFP+ population of cells. BM from Csf1r-EGFP sheep was isolated from ribs and analyzed via flow cytometry. (A) Gating strategy. Dead cells were excluded with propidium iodide staining and granulocytes excluded via FSC/SSC. EGFPlo and EGFPhi were selected once quadrants were set with negative control BM. (B) Analysis of CD14, CD16, and CD172a expression in GFPlo and GFPhi cells. Quadrants were set using isotype controls for each population. (C) Leishman-stained EGFP+ cells (combined EGFPlo and EGFPhi). Scale bar, 10 μm. Dot plots and Leishman staining of EGFP+ cells representative of five and two sheep, respectively. Leishman staining was representative of 15 images per animal.
From: Pridans C, Davis GM, Sauter KA, Lisowski ZM, Corripio-Miyar Y, Raper A, Lefevre L, Young R, McCulloch ME, Lillico S, Milne E, Whitelaw B, Hume DA.
A Csf1r-EGFP Transgene Provides a Novel Marker for Monocyte Subsets in Sheep.
J Immunol. 2016 Sep 15;197(6):2297-305.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Bovine CD172a antibody, clone CC149 (MCA2041GA) used for the evaluation of CD172a expression on ovine bone marrow derived, monocyte derived and alveolar macrophages by flow cytometry.
Image caption:
Flow cytometry analysis of AMs, MDMs, and BMDMs from sheep. A lung lavage was performed to isolate AMs. Macrophages were differentiated from PBMCs and BM for 7 d with rhCSF1. Dead cells were excluded with propidium iodide. Solid lines represent isotype controls. Histograms are representative of three (AM and MDM) or five (BMDM) sheep.
From: Pridans C, Davis GM, Sauter KA, Lisowski ZM, Corripio-Miyar Y, Raper A, Lefevre L, Young R, McCulloch ME, Lillico S, Milne E, Whitelaw B, Hume DA.
A Csf1r-EGFP Transgene Provides a Novel Marker for Monocyte Subsets in Sheep.
J Immunol. 2016 Sep 15;197(6):2297-305.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Bovine CD172a antibody, clone CC149 (MCA2041GA) used for the evaluation of CD172a expression on ovine peripheral blood monocytes by flow cytometry.
Image caption:
Monocytes are EGFP+ in Csf1r-EGFP sheep. Peripheral blood monocytes were isolated from the blood of live animals and analyzed via flow cytometry. (A) EGFP+ gating strategy. (B) Expression of CD14, CD16, and CD172a in EGFPlo and EGFPhi PBMCs. Quadrants were set using isotype controls for each population. (C) Leishman-stained EGFP+ cells. Scale bars, 10 μm. Dot plots and Leishman staining of EGFP+ cells are representative of nine and three sheep, respectively. Leishman staining was representative of 10 images per animal.
From: Pridans C, Davis GM, Sauter KA, Lisowski ZM, Corripio-Miyar Y, Raper A, Lefevre L, Young R, McCulloch ME, Lillico S, Milne E, Whitelaw B, Hume DA.
A Csf1r-EGFP Transgene Provides a Novel Marker for Monocyte Subsets in Sheep.
J Immunol. 2016 Sep 15;197(6):2297-305.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Bovine CD172α antibody, clone CC149 (MCA2041GA) used for the detection of SIRP1α expressing cells in bovine PBMCs by flow cytometry.
Image caption:
Expansion of CD172a+ cells in response to ESAT-6/CFP-10 stimulation. Isolated PBMC were stained with PKH67 and cultured for 6d with media only, rMPB83, a pool of overlapping ESAT-6 and CFP-10 peptides, or rESAT-6:CFP-10. Data are depicted as: (A) dot plots (CD172a-PE (y-axis) versus PKH67 (x-axis, green fluorescence), (B) histograms generated by Modfit Proliferation Wizard analysis of PKH67 staining intensity (gated on CD172a-PE+ cells within the live gate) and (C) mean (±SEM) percent CD172a+ cells within PBMC cultures (stimulation indicated in the lower margin) from non-infected (open bars, n = 9, includes non- (n = 3), BCG- (n = 3), and ΔRD1- (n = 3) vaccinates) or M. bovis-infected (closed bars, n = 20) cattle. Responses did not differ between controls, BCG- and ΔRD1- vaccinates; thus, these groups were combined. Gate R2 in panel A highlights the CD172a+, PKH67lo proliferative fraction. For panels A and B, data from a single M. bovis-infected animal are provided that are indicative of a representative response.
From: Waters WR, Palmer MV, Nonnecke BJ, Thacker TC, Estes DM, et al. (2009)
Signal Regulatory Protein α (SIRPα)+ Cells in the Adaptive Response to ESAT-6/CFP-10 Protein of Tuberculous Mycobacteria.
PLoS ONE 4(7): e6414.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Bovine CD172α antibody, clone CC149 (MCA2041G) used for the detection of SIRP1α expressing cells in cultured bovine PBMCs by immunofluorescence.
Image caption:
CD172a cells bind rESAT-6:CFP-10 and M. bovis BCG. CD172a+ cells (PE-labeled) were isolated by high-speed cell sorting (>98% purity) from rESAT-6:CFP-10-stimulated PBMC 6d cultures; incubated with rESAT-6:CFP-10-FITC or BCG-FITC for 2–96 hrs; and evaluated by fluorescence microscopy. (A) rESAT-6:CFP-10-FITC bound to the surface of CD172a+ cells in a focal pattern (24 hr cultures shown). Labeling patterns were similar at 2, 24 (shown in Fig. 3A) and 96 hrs after addition of rESAT-6:CFP-10 (FITC) to cells, except for increased polarization of staining at 96 hrs. (B) Over the 96 hr culture period, PE-staining (red) used for CD172a+ cell sorting persisted (24 hr cultures shown) with a polar distribution, possibly due to capping of antibody bound to CD172a. However, rESAT-6:CFP-10-labeling (green) did not overlap with CD172a-PE labeling (red). (C) M. bovis BCG was detected in association with CD172a+ cells at 2 (not shown) and 24 hrs (green, intact bacteria associated with the cell surface, panel C) after addition of the live bacteria to the CD172a+ cell culture and by 96 hrs, M. bovis BCG was internalized and mostly degraded (D). White bar = 10 μm.
From: Waters WR, Palmer MV, Nonnecke BJ, Thacker TC, Estes DM, et al. (2009)
Signal Regulatory Protein α (SIRPα)+ Cells in the Adaptive Response to ESAT-6/CFP-10 Protein of Tuberculous Mycobacteria.
PLoS ONE 4(7): e6414.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Bovine CD172a antibody, clone CC149 (MCA2041GA) used for the identification of CD172a expressing cells in bovine blood by flow cytometry.
Image caption:
Adaptation of the reverse-transmigration in vitro model with bovine cells to recapitulate monocyte-derived cells mobilization from blood to the draining lymph node under inflammatory condition. A-D Schematic representation of the in vitro differentiation of blood monocytes into macrophages and Mo-DCs using the reverse-transmigration model. E-I Two-color flow cytometry assay of monocyte-derived cells collected from monocyte/BAEC cultures grown on collagen-coated transwells according to the reverse-transmigration protocol. Non-adherent and low-adherent cells within the top and bottom sections of the transwell were processed for cytometry analysis. Left contour plots show the conditions in the absence of any exogenous stimulus whereas right contour plots show conditions with zymosan. Cells were labeled with anti-SIRP1-α to discriminate monocyte-derived cells from endothelial cells. The expression of MHCII within the SIRP1-α+ population was also determined to discriminate within monocyte-derived cells potential macrophages (MHCII+) and potential Mo-DC (MHCIIhi) (E). The percentages of SIRP1-α+MHCII+, SIRP1-α+MHCIIhi and BAECs in each representative dot plot are indicated (E). F Comparison showing the relative number of SIRP1-α+MHCII+ cells in the bottom of the transwell in conditions with or without zymosan stimulation. G Comparison showing the relative number of SIRP1-α+MHCII+ cells in the top section of the transwell in conditions with or without zymosan stimulation. H Comparison showing the relative number of SIRP1-α+MHCIIhi cells in the top section of the transwell in conditions with or without zymosan stimulation. i Comparison showing the relative expression level of MHCII in SIRP1-α+MHCII+ cells in the top section of the transwell in conditions with or without zymosan stimulation. F-I The value of the control condition was arbitrarily set to 1. Data are presented as the mean ± SD based on four independent experiments each including at least three transwells per condition. Statistically significant differences between the two groups were determined by the Student's t test, *P <0.05 and **P <0.005.
From: Vachiery N, Puech C, Cavelier P, Rodrigues V, Aprelon R, Lefrançois T, Martinez D, Epardaud M. An in vitro model to assess the immunosuppressive effect of tick saliva on the mobilization of inflammatory monocyte-derived cells.
Vet Res. 2015 Sep 28;46(1):117.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Bovine CD172a antibody, clone CC149 (MCA2041GA) used for the identification of CD172a expressing cells in bovine blood by flow cytometry.
Image caption:
In vitro characterization of the dual immunosuppressive effect of tick saliva on inflammatory-induced mobilization of monocyte-derived mononuclear phagocytes. A-E Two-color flow cytometry assay of monocyte-derived cells collected from monocyte/BAEC cultures grown on collagen-coated transwells for 48 h according to the reverse-transmigration protocol. Left contour plots show conditions with zymosan alone whereas right contour plots show conditions with zymosan and tick saliva. B Comparison showing the relative number of SIRP1-α+MHCII+ cells in the bottom of the transwell in conditions with zymosan alone or with zymosan and saliva. C Comparison showing the relative number of SIRP1-α+MHCII+ cells in the top section of the transwell in conditions with zymosan alone or with zymosan and saliva. D Comparison showing the relative number of SIRP1-α+MHCIIhi cells in the top section of the transwell in conditions with zymosan alone or with zymosan and saliva. E Comparison showing the relative expression level of MHCII in SIRP1-α+MHCII+ cells in the top section of the transwell in conditions with zymosan alone or with zymosan and saliva. B-E The value for the control condition (zymosan alone) was arbitrarily set to 1. Data are presented as the mean± SD based on four independent experiments each including at least three transwells per condition. Statistically significant differences between the two groups were determined by the Student's t test, *P < 0.05 and **P <0.005. (F&G) Representative histograms of MHCII and CD86 staining on SIRPα+ monocyte-derived cells incubated for 48 h with zymosan and saliva at amounts varying from 0 to 55 μg/mL. One representative experiment out of two independent experiments is shown. G Schematic representation of the effect of tick saliva on the mobilization of monocyte-derived APCs in vivo. (1) Monocytes from the blood are recruited to the area of the tick bite where a proportion of the cells differentiate into potential macrophages. (2) Potential Mo-DC precursors then migrate from this area into the draining lymphatic vessels.
From: Vachiery N, Puech C, Cavelier P, Rodrigues V, Aprelon R, Lefrançois T, Martinez D, Epardaud M.
An in vitro model to assess the immunosuppressive effect of tick saliva on the mobilization of inflammatory monocyte-derived cells.
Vet Res. 2015 Sep 28;46(1):117.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
PE-Cy5 conjugated Mouse anti Bovine CD172a antibody, clone KD1 (MCA2041C) used to identify monocyte populations in bovime blood samples by flow cytometry.
Image caption:
Changes in the monocyte subsets of PBMCs in Holstein and Jersey steers subjected to heat stress. Flow cytometry analysis to identify monocyte subset population. A CD172a+ monocyte cells were sorted from total PBMCs. MT indicates moderate THI condition and HT indicates high THI condition
From: Park DS, Gu BH, Park YJ, Joo SS, Lee SS, Kim SH, Kim ET, Kim DH, Lee SS, Lee SJ, Kim BW, Kim M.
Dynamic changes in blood immune cell composition and function in Holstein and Jersey steers in response to heat stress.
Cell Stress Chaperones. 2021 Jul;26(4):705-20.
doi: 10.1007/s12192-021-01216-2.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Bovine CD172a antibody, clone CC149 (MCA2041GA) used to identify CD172a expressing cells in bovine intestinal tissue by immunohistochemistry on cryosections.
Image caption:
Immunohistochemical staining of intestinal tissues from MAP-infected and uninfected cows. (1) Representative tissue sections stained for CD172a myeloid cells (brown stain) from MAP-infected (A, F) and uninfected (B, G) ileum (A, B) and jejunum (F, G).
From: Ibeagha-Awemu EM, Bissonnette N, Do DN, Dudemaine PL, Wang M, Facciuolo A, Griebel P.
Regionally Distinct Immune and Metabolic Transcriptional Responses in the Bovine Small Intestine and Draining Lymph Nodes During a Subclinical Mycobacterium avium subsp. paratuberculosis Infection.
Front Immunol. 2021 Dec 15;12:760931.
doi: 10.3389/fimmu.2021.760931.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
F IF C ResetMouse anti Bovine CD172a
- Product Type
- Monoclonal Antibody
- Clone
- CC149
- Isotype
- IgG2b
- Specificity
- CD172a
Antibody Quality and Performance Guarantee
| Mouse anti Bovine CD172a antibody, clone CC149 recognizes bovine CD172a, also known as MyD-1 antigen and SIRPA. CD172a is a ~55 kDa single pass type 1 membrane protein belonging to the family of signal regulatory proteins (SIRP). CD172a has been identified as the receptor for CD47. Bovine CD172a is strongly expressed by splenic macrophages, monocytes and a subset of afferent lymph veiled cells (ALVC) and by dendritic cells in the skin. |
- Target Species
- Bovine
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Bovine afferent veiled dendritic cells
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/100 | 1/200 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
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References for CD172a antibody
-
Smith, R. et al. (2003) A novel MyD-1 (SIRP-1alpha) signaling pathway that inhibits LPS-induced TNFalpha production by monocytes.
Blood.102: 2532-40. -
Brackenbury, L.S. et al. (2005) Identification of a cell population that produces alpha/beta interferon in vitro and in vivo in response to noncytopathic bovine viral diarrhea virus.
J Virol. 79: 7738-44. -
Price, S.J. & Hope, J.C.. (2009) Enhanced secretion of interferon-gamma by bovine gammadelta T cells induced by coculture with Mycobacterium bovis-infected dendritic cells: evidence for reciprocal activating signals.
Immunology. 126:201-8 -
Waters, W.R. (2009) Signal regulatory protein alpha (SIRPalpha) cells in the adaptive response to ESAT-6/CFP-10 protein of tuberculous mycobacteria.
PLoS One. 4: e6414. -
Jensen, K. et al. (2014) Comparison of small interfering RNA (siRNA) delivery into bovine monocyte-derived macrophages by transfection and electroporation.
Vet Immunol Immunopathol. 158 : 224-32. -
Hussen J et al. (2014) The chemokine CCL5 induces selective migration of bovine classical monocytes and drives their differentiation into LPS-hyporesponsive macrophages in vitro.
Dev Comp Immunol. 47 (2): 169-77. -
Eger, M. et al. (2015) Impacts of parturition and body condition score on glucose uptake capacity of bovine monocyte subsets.
Vet Immunol Immunopathol. 166 (1-2): 33-42. -
Vachiery N et al. (2015) An in vitro model to assess the immunosuppressive effect of tick saliva on the mobilization of inflammatory monocyte-derived cells.
Vet Res. 46 (1): 117.
View The Latest Product References
-
Tahoun, A. et al. (2015) Functional analysis of bovine TLR5 and association with IgA responses of cattle following systemic immunisation with H7 flagella.
Vet Res. 46: 9. -
Pridans, C. et al. (2016) A Csf1r-EGFP Transgene Provides a Novel Marker for Monocyte Subsets in Sheep.
J Immunol. 197 (6): 2297-305. -
Herry, V. et al. (2017) Local immunization impacts the response of dairy cows to Escherichia coli mastitis.
Sci Rep. 7 (1): 3441. -
Liu, J. et al. (2020) Theileria annulata. transformation altered cell surface molecules expression and endocytic function of monocyte-derived dendritic cells.
Ticks Tick Borne Dis. 11 (3): 101365. -
Okino, C.H. et al. (2020) A polymorphic CD4 epitope related to increased susceptibility to Babesia bovis in Canchim calves.
Vet Immunol Immunopathol. 230: 110132. -
Barut, G.T. et al. (2020) Transcriptomic profiling of bovine blood dendritic cells and monocytes following TLR stimulation.
Eur J Immunol. 50 (11): 1691-711. -
Kolar, Q.K. et al. (2020) Anatomical distribution of respiratory tract leukocyte cell subsets in neonatal calves.
Vet Immunol Immunopathol. 227: 110090. -
Park, D.S. et al. (2021) Dynamic changes in blood immune cell composition and function in Holstein and Jersey steers in response to heat stress.
Cell Stress Chaperones. 26 (4): 705-20. -
Ibeagha-Awemu, E.M. et al. (2021) Regionally Distinct Immune and Metabolic Transcriptional Responses in the Bovine Small Intestine and Draining Lymph Nodes During a Subclinical Mycobacterium avium subsp. paratuberculosis Infection.
Front Immunol. 12: 760931. -
Marzo, S. et al. (2022) Characterisation of dendritic cell frequency and phenotype in bovine afferent lymph reveals kinetic changes in costimulatory molecule expression.
Vet Immunol Immunopathol. 243: 110363. -
Casaro, S. et al. (2022) Flow cytometry panels for immunophenotyping dairy cattle peripheral blood leukocytes
Vet Immunol Immunopathol. 248: 110417.
Further Reading
-
Howard, C.J. et al. (1999) Dendritic cells in cattle: phenotype and function.
Vet Immunol Immunopathol. 72 (1-2): 119-24.
- Synonyms
- SIRP ALPHA
- UniProt
- O46631
- Entrez Gene
- SIRPA
- GO Terms
- GO:0016021 integral to membrane
- GO:0017124 SH3 domain binding
MCA2041GA
155742
1709
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