CD172a antibody | CC149

Product Code Applications Pack Size List Price Your Price Qty
MCA2041GA
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SDS Safety Datasheet SDS
F 0.1 mg loader
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loader
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Mouse anti Bovine CD172a antibody, clone CC149 recognizes bovine CD172a, also known as MyD-1 antigen and SIRPA.

CD172a is a ~55 kDa single pass type 1 membrane protein belonging to the family of signal regulatory proteins (SIRP). CD172a has been identified as the receptor for CD47. Bovine CD172a is strongly expressed by splenic macrophages, monocytes and a subset of afferent lymph veiled cells (ALVC) and by dendritic cells in the skin.

Target Species
Bovine
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Bovine afferent veiled dendritic cells
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/100 1/200
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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References for CD172a antibody

  1. Howard, C.J. et al. (1999) Dendritic cells in cattle: phenotype and function.
    Vet Immunol Immunopathol. 72 (1-2): 119-24.
  2. Price, S.J. & Hope, J.C.. (2009) Enhanced secretion of interferon-gamma by bovine gammadelta T cells induced by coculture with Mycobacterium bovis-infected dendritic cells: evidence for reciprocal activating signals.
    Immunology. 126:201-8
  3. Waters, W.R. (2009) Signal regulatory protein alpha (SIRPalpha) cells in the adaptive response to ESAT-6/CFP-10 protein of tuberculous mycobacteria.
    PLoS One. 4: e6414.
  4. Brackenbury, L.S. et al. (2005) Identification of a cell population that produces alpha/beta interferon in vitro and in vivo in response to noncytopathic bovine viral diarrhea virus.
    J Virol. 79: 7738-44.
  5. Smith, R. et al. (2003) A novel MyD-1 (SIRP-1alpha) signaling pathway that inhibits LPS-induced TNFalpha production by monocytes.
    Blood.102: 2532-40.
  6. Jensen, K. et al. (2014) Comparison of small interfering RNA (siRNA) delivery into bovine monocyte-derived macrophages by transfection and electroporation.
    Vet Immunol Immunopathol. 158 : 224-32.
  7. Tahoun, A. et al. (2015) Functional analysis of bovine TLR5 and association with IgA responses of cattle following systemic immunisation with H7 flagella.
    Vet Res. 46: 9.
  8. Hussen J et al. (2014) The chemokine CCL5 induces selective migration of bovine classical monocytes and drives their differentiation into LPS-hyporesponsive macrophages in vitro.
    Dev Comp Immunol. 47 (2): 169-77.
  9. View The Latest Product References
  10. Eger, M. et al. (2015) Impacts of parturition and body condition score on glucose uptake capacity of bovine monocyte subsets.
    Vet Immunol Immunopathol. 166 (1-2): 33-42.
  11. Vachiery N et al. (2015) An in vitro model to assess the immunosuppressive effect of tick saliva on the mobilization of inflammatory monocyte-derived cells.
    Vet Res. 46 (1): 117.
  12. Pridans, C. et al. (2016) A Csf1r-EGFP Transgene Provides a Novel Marker for Monocyte Subsets in Sheep.
    J Immunol. 197 (6): 2297-305.
  13. Herry, V. et al. (2017) Local immunization impacts the response of dairy cows to Escherichia coli mastitis.
    Sci Rep. 7 (1): 3441.
  14. Okino, C.H. et al. (2020) A polymorphic CD4 epitope related to increased susceptibility to Babesia bovis in Canchim calves.
    Vet Immunol Immunopathol. 230: 110132.
  15. Park, D.S. et al. (2021) Dynamic changes in blood immune cell composition and function in Holstein and Jersey steers in response to heat stress.
    Cell Stress Chaperones. 26 (4): 705-20.
  16. Marzo, S. et al. (2022) Characterisation of dendritic cell frequency and phenotype in bovine afferent lymph reveals kinetic changes in costimulatory molecule expression.
    Vet Immunol Immunopathol. 243: 110363.
  17. Casaro, S. et al. (2022) Flow cytometry panels for immunophenotyping dairy cattle peripheral blood leukocytes
    Vet Immunol Immunopathol. 248: 110417.
  18. Barut, G.T. et al. (2020) Transcriptomic profiling of bovine blood dendritic cells and monocytes following TLR stimulation.
    Eur J Immunol. 50 (11): 1691-711.
  19. Kolar, Q.K. et al. (2020) Anatomical distribution of respiratory tract leukocyte cell subsets in neonatal calves.
    Vet Immunol Immunopathol. 227: 110090.
  20. Liu, J. et al. (2020) Theileria annulata. transformation altered cell surface molecules expression and endocytic function of monocyte-derived dendritic cells.
    Ticks Tick Borne Dis. 11 (3): 101365.
  21. Ibeagha-Awemu, E.M. et al. (2021) Regionally Distinct Immune and Metabolic Transcriptional Responses in the Bovine Small Intestine and Draining Lymph Nodes During a Subclinical Mycobacterium avium subsp. paratuberculosis Infection.
    Front Immunol. 12: 760931.

Immunofluorescence

Synonyms
SIRP ALPHA
UniProt
O46631
Entrez Gene
SIRPA
GO Terms
GO:0016021 integral to membrane
GO:0017124 SH3 domain binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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