CD25 antibody | AbD13504

Human anti Chicken CD25:FITC

Product Type
Monoclonal Antibody
Clone
AbD13504
Isotype
HuCAL Fab bivalent
Specificity
CD25

Product Code Applications Pack Size List Price Your Price Qty
HCA173F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
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loader
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Human anti Chicken CD25 antibody, clone AbD13504 recognizes the chicken IL-2 receptor alpha chain (IL-2Rα) , identified as the chicken homologue of human CD25 and cited as ChCD25 in published literature.
Chicken IL-2Rα is a cell surface antigen expressed by activated T-lymphocytes, monocytes/macrophages and thrombocytes.
CD4+ve/CD25+ve and CD8+ve/CD25+ve cells are up-regulated following infection with avian influenza virus (H9N2) and may therefore have an important role in the immune response to avian influenza virus infection (Huang et al. 2012).
Chicken IL-2Rα is the low affinity receptor for Interleukin-2.

Target Species
Chicken
Species Cross-Reactivity
Target SpeciesCross Reactivity
Duck
Goose
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. Conjugated to fluorescein isothiocyanate (FITC) - liquid.
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Recombinantly expressed his-tagged chicken CD25
Approx. Protein Concentrations
Antibody concentration 0.1 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual

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References for CD25 antibody

  1. Braukmann, M. et al. (2015) Avian CD25(+) gamma/delta (γδ) T cells after Salmonella exposure.
    Vet Immunol Immunopathol. 168 (1-2): 14-8.
  2. Polasky C et al. (2016) Non-specific activation of CD8α-characterised γδ T cells in PBL cultures of different chicken lines.
    Vet Immunol Immunopathol. 179: 1-7.
  3. Lee, I.K. et al. (2018) Regulation of CD4+CD8-CD25+ and CD4+CD8+CD25+ T cells by gut microbiota in chicken.
    Sci Rep. 8 (1): 8627.

Synonyms
IL-2 Receptor Alpha
UniProt
Q90WJ4
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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