CD25 antibody | AbD13504
Human anti Chicken CD25:FITC
- Product Type
- Monoclonal Antibody
- Clone
- AbD13504
- Isotype
- HuCAL Fab bivalent
- Specificity
- CD25
Human anti Chicken CD25 antibody, clone AbD13504 recognizes the chicken IL-2 receptor alpha chain (IL-2Rα) , identified as the chicken homologue of human CD25 and cited as ChCD25 in published literature. Chicken IL-2Rα is a cell surface antigen expressed by activated T-lymphocytes, monocytes/macrophages and thrombocytes. CD4+ve/CD25+ve and CD8+ve/CD25+ve cells are up-regulated following infection with avian influenza virus (H9N2) and may therefore have an important role in the immune response to avian influenza virus infection (Huang et al. 2012). Chicken IL-2Rα is the low affinity receptor for Interleukin-2. |
- Target Species
- Chicken
- Species Cross-Reactivity
-
Target Species Cross Reactivity Duck Goose - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL phage display library, expressed in E.coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. Conjugated to fluorescein isothiocyanate (FITC) - liquid.
- Preparation
- Metal chelate affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Immunogen
- Recombinantly expressed his-tagged chicken CD25
- Approx. Protein Concentrations
- Antibody concentration 0.1 mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only.
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/10 |
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
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Mouse anti Chicken CD25 | MCA5925GA | E F WB | 0.1 mg | Log in | |||
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Description | Mouse anti Chicken CD25 |
References for CD25 antibody
-
Braukmann, M. et al. (2015) Avian CD25(+) gamma/delta (γδ) T cells after Salmonella exposure.
Vet Immunol Immunopathol. 168 (1-2): 14-8. -
Polasky C et al. (2016) Non-specific activation of CD8α-characterised γδ T cells in PBL cultures of different chicken lines.
Vet Immunol Immunopathol. 179: 1-7. -
Lee, I.K. et al. (2018) Regulation of CD4+CD8-CD25+ and CD4+CD8+CD25+ T cells by gut microbiota in chicken.
Sci Rep. 8 (1): 8627. -
He, S. et al. (2021) High-frequency and activation of CD4+CD25+ T cells maintain persistent immunotolerance induced by congenital ALV-J infection.
Vet Res. 52 (1): 119. -
Rimondi, A. et al. (2022) Few Amino Acid Mutations in H6 Influenza A Virus From South American Lineage Increase Viral Replication Efficiency in Poultry.
Front Microbiol. 13: 953738.
- Synonyms
- IL-2 Receptor Alpha
- UniProt
- Q90WJ4
- Licensed Use
- For in vitro. research purposes only, unless otherwise specified in writing by Bio-Rad.
HCA173F
150715 159731 1709If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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