CD45 antibody | UM16-6

Mouse anti Chicken CD45:RPE
- Product Type
- Monoclonal Antibody
- Clone
- UM16-6
- Isotype
- IgG2a
- Specificity
- CD45
Quick Links:
Mouse anti Chicken CD45 antibody, clone UM16-6 recognizes chicken CD45, also known as leucocyte common antigen. CD45 is a heavily-glycosylated transmembrane protein tyrosine phosphatase (PTPase) expressed by all nucleated cells of haematopoietic origin. Variation in the expression of a particular CD45 isoform, is regulated during the haematopoietic development of the different cell lineages. CD45 is essential for antigen-induced signal transduction through the antigen receptor and as with other PTPase family members, acts in balance with protein tyrosine kinases, causing the dephosphorylation of negative regulatory tyrosine sites. De-phosphorylation by CD45, is required for the activation of the src-family kinases p56lck and p59fyn. Chicken CD45 has an additional cysteine residue near the transmembrane region compared to human and shark CD45 (Okumura et al. 1996). The overall domain structure between mammalian and chicken CD45 appears to be conserved, but the sequence homology between the extracellular regions is very low. |
- Target Species
- Chicken
- Species Cross-Reactivity
-
Target Species Cross Reactivity Turkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Immunogen
- COS cells transfected with CD45 gene.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) RPE 488nm laser 496 578 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
After reconstitution store at +4oC.
DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | ![]() |
Neat | 1/5 |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
References for CD45 antibody
-
Pavlova, S. et al. (2010) In vitro and in vivo characterization of glycoprotein C-deleted infectious laryngotracheitis virus.
J Gen Virol. 91:847-57. -
Wattrang, E. et al. (2015) CD107a as a marker of activation in chicken cytotoxic T cells.
J Immunol Methods. 419: 35-47. -
Reddy SK et al. (2008) The BAFF-Interacting receptors of chickens.
Dev Comp Immunol. 32 (9): 1076-87. -
Ulrich-Lynge SL et al. (2015) The consequence of low mannose-binding lectin plasma concentration in relation to susceptibility to Salmonella infantis in chickens.
Vet Immunol Immunopathol. 163 (1-2): 23-32. -
Eren, U. et al. (2016) The several elements of intestinal innate immune system at the beginning of the life of broiler chicks.
Microsc Res Tech. 79 (7): 604-14. -
Röhe, I. et al. (2017) Effect of feeding soybean meal and differently processed peas on the gut mucosal immune system of broilers
Poultry Science. Feb 23 [Epub ahead of print] -
Czerwiński, J. et al. (2015) The use of genetically modified Roundup Ready soyabean meal and genetically modified MON 810 maize in broiler chicken diets.
Part 1. Effects on performance and blood lymphocyte subpopulations
J Anim Feed Sci 24: 134-43. -
Kjærup, R.B. et al. (2017) Comparison of growth performance and immune parameters of three commercial chicken lines used in organic production.
Vet Immunol Immunopathol. 187: 69-79.
View The Latest Product References
-
Larsen, F.T. et al. (2019) Immunoprofiling of peripheral blood from infectious bronchitis virus vaccinated MHC-B chicken lines - Monocyte MHC-II expression as a potential correlate of protection.
Dev Comp Immunol. 96: 93-102. -
Naghizadeh, M. et al. (2019) Rapid whole blood assay using flow cytometry for measuring phagocytic activity of chicken leukocytes.
Vet Immunol Immunopathol. 207: 53-61. -
Fenzl, L. et al. (2017) γδ T cells represent a major spontaneously cytotoxic cell population in the chicken.
Dev Comp Immunol. 73: 175-83. -
Harvanová, D. et al. (2014) Isolation, cultivation and characterisation of pigeon osteoblasts seeded on xenogeneic demineralised cancellous bone scaffold for bone grafting.
Vet Res Commun. 38 (3): 221-8.
Further Reading
-
Symons, A. et al. (1999) Domain organization of the extracellular region of CD45.
Protein Eng. 12 (10): 885-92. -
Okumura, M. et al. (1996) Comparison of CD45 extracellular domain sequences from divergent vertebrate species suggests the conservation of three fibronectin type III domains.
J Immunol. 157 (4): 1569-75. -
Jung, E.J. et al. (1997) Phosphorylation of chicken protein tyrosine phosphatase 1 by casein kinase II in vitro.
Experimental and Molecular Medicine 29(4): 229-33.
- Synonyms
- Leucocyte Common Antigen
- RRID
- AB_808433
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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