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CD45 antibody | UM16-6

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Mouse anti Chicken CD45:RPE

Product Type
Monoclonal Antibody
Clone
UM16-6
Isotype
IgG2a
Specificity
CD45

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Mouse anti Chicken CD45 antibody, clone UM16-6 recognizes chicken CD45, also known as leucocyte common antigen. CD45 is a heavily-glycosylated transmembrane protein tyrosine phosphatase (PTPase) expressed by all nucleated cells of haematopoietic origin. Variation in the expression of a particular CD45 isoform, is regulated during the haematopoietic development of the different cell lineages.

CD45 is essential for antigen-induced signal transduction through the antigen receptor and as with other PTPase family members, acts in balance with protein tyrosine kinases, causing the dephosphorylation of negative regulatory tyrosine sites. De-phosphorylation by CD45, is required for the activation of the src-family kinases p56lck and p59fyn.

Chicken CD45 has an additional cysteine residue near the transmembrane region compared to human and shark CD45 (Okumura et al. 1996). The overall domain structure between mammalian and chicken CD45 appears to be conserved, but the sequence homology between the extracellular regions is very low.

Target Species
Chicken
Species Cross-Reactivity
Target SpeciesCross Reactivity
Turkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
COS cells transfected with CD45 gene.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/5
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

How to Use the Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

References for CD45 antibody

  1. Pavlova, S. et al. (2010) In vitro and in vivo characterization of glycoprotein C-deleted infectious laryngotracheitis virus.
    J Gen Virol. 91:847-57.
  2. Wattrang, E. et al. (2015) CD107a as a marker of activation in chicken cytotoxic T cells.
    J Immunol Methods. 419: 35-47.
  3. Reddy SK et al. (2008) The BAFF-Interacting receptors of chickens.
    Dev Comp Immunol. 32 (9): 1076-87.
  4. Ulrich-Lynge SL et al. (2015) The consequence of low mannose-binding lectin plasma concentration in relation to susceptibility to Salmonella infantis in chickens.
    Vet Immunol Immunopathol. 163 (1-2): 23-32.
  5. Eren, U. et al. (2016) The several elements of intestinal innate immune system at the beginning of the life of broiler chicks.
    Microsc Res Tech. 79 (7): 604-14.
  6. Röhe, I. et al. (2017) Effect of feeding soybean meal and differently processed peas on the gut mucosal immune system of broilers
    Poultry Science. Feb 23 [Epub ahead of print]
  7. Czerwiński, J. et al. (2015) The use of genetically modified Roundup Ready soyabean meal and genetically modified MON 810 maize in broiler chicken diets. Part 1. Effects on performance and blood lymphocyte subpopulations
    J Anim Feed Sci 24: 134-43.
  8. Kjærup, R.B. et al. (2017) Comparison of growth performance and immune parameters of three commercial chicken lines used in organic production.
    Vet Immunol Immunopathol. 187: 69-79.
    9. View The Latest Product References
  9. Larsen, F.T. et al. (2019) Immunoprofiling of peripheral blood from infectious bronchitis virus vaccinated MHC-B chicken lines - Monocyte MHC-II expression as a potential correlate of protection.
    Dev Comp Immunol. 96: 93-102.
  10. Naghizadeh, M. et al. (2019) Rapid whole blood assay using flow cytometry for measuring phagocytic activity of chicken leukocytes.
    Vet Immunol Immunopathol. 207: 53-61.
  11. Fenzl, L. et al. (2017) γδ T cells represent a major spontaneously cytotoxic cell population in the chicken.
    Dev Comp Immunol. 73: 175-83.
  12. Harvanová, D. et al. (2014) Isolation, cultivation and characterisation of pigeon osteoblasts seeded on xenogeneic demineralised cancellous bone scaffold for bone grafting.
    Vet Res Commun. 38 (3): 221-8.

Further Reading

  1. Symons, A. et al. (1999) Domain organization of the extracellular region of CD45.
    Protein Eng. 12 (10): 885-92.
  2. Okumura, M. et al. (1996) Comparison of CD45 extracellular domain sequences from divergent vertebrate species suggests the conservation of three fibronectin type III domains.
    J Immunol. 157 (4): 1569-75.
  3. Jung, E.J. et al. (1997) Phosphorylation of chicken protein tyrosine phosphatase 1 by casein kinase II in vitro.
    Experimental and Molecular Medicine 29(4): 229-33.

Synonyms
Leucocyte Common Antigen
RRID
AB_808433
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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