Cytokeratin 19 Antibody | BA17

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Cytokeratin 19 Antibody | BA17 gallery image 1

Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human cytokeratin 19 antibody (MCA2145) followed by HRP polymer detection and DAB substrate development following heat mediated antigen retrieval using citrate buffer at pH6.2 (low power)

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Cytokeratin 19 Antibody | BA17 gallery image 2

Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human cytokeratin 19 antibody (MCA2145) followed by HRP polymer detection and DAB substrate development following heat mediated antigen retrieval using citrate buffer at pH6.2 (high power)

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Cytokeratin 19 Antibody | BA17 gallery image 3

Sandwich ELISA analysis of human cytokeratin-19 expression using Mouse anti Human cytokeratin 19 antibody, clone BA19 (MCA2145) as a capture reagent and biotinylated Mouse anti Human cytokeratin 19 antibody, clone A53-B/A2 (5552-9009) as a detection reagent with purified human cytokeratin 19 (Bio-rad catalog; 34002301) as antigen to create the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Normal serum (green), colorectal cancer serum (orange) and breast cancer serum (red) samples at 1:4 dilution are shown

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  • Mouse anti Human Cytokeratin 19
  • Mouse anti Human Cytokeratin 19
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    BA17
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2145C, P *, WBdatasheet pdfdatasheet pdf0.2 mg
    MCA2145
    MCA2145TC, P *datasheet pdfdatasheet pdf20 µg
    MCA2145T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Human Cytokeratin 19 antibody, clone BA17 recognizes the 40kDa cytokeratin corresponding to cytokeratin 19.

      Cytokeratin 19 is expressed by many simple and non-keratinizing normal and neoplastic epithelia, including glandular and ductal epithelia.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
    • Immunogen
      Mammary organoids.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Western Blotting
      Immunohistology - Paraffin(1)1/501/100
      (1)
      This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Western Blotting
      Immunohistology - Paraffin(1)1/501/100
      (1)
      This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
      Normal breast
    • Histology Positive Control Tissue
      Normal breast
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Cytokeratin 19 Antibody Formats

    Formats Clone Applications Sizes available
    Cytokeratin 19 Antibody : Purified BA17 C, P *, WB 20 µg | 0.2 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
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      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
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      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
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      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
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      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
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      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
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      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
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      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Normal breast
              Normal breast

              References

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