IL-4 Antibody | CC303

//www.bio-rad-antibodies.com/cow-bovine-il-4-antibody-cc303-mca1820.html
IL-4 Antibody | CC303 gallery image 1

Published customer image:
Mouse anti Bovine interleukin-4 antibody, clone CC303 used for the identification of interferonγ expressing dog lymphocytes by flow cytometry.
Image caption:
Representative dot plots illustrating the analysis of intracellular cytokine profile in T-cell subsets. (A) Pseudocolor plot distribution of short-term in vitro cultured (control or SLA-Ag stimulated) canine whole blood sample according to cell size (Forward scatter - FSC) and granularity (Side scatter- SSC) used for lymphocyte gate selection (R1) of FSCLowSSCLow events. (B) Pseudocolor plots representing cytokines?+?(IL-17, TNF-a, IFN-?, TGF-ß and IL-4) CD4+ cells within gated lymphocytes and (C) Pseudocolor plots representing cytokines?+?(IL-17, TNF-a, IFN-?, TGF-ß and IL-4) CD8+ cells within gated lymphocytes. The frequency of cytokines+ T-cells subsets were calculated by quadrant statistics approach and first reported as percentage of gated lymphocytes prior to the calculation of the SLAg/Control indexes.

From: Costa-Pereira C, Moreira ML, Soares RP, Marteleto BH, Ribeiro VM, França-Dias MH, Cardoso LM, Viana KF, Giunchetti RC, Martins-Filho OA, Araújo MS. One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasis. BMC Vet Res. 2015 Apr 11;11(1):92.

Enlarge
IL-4 Antibody | CC303 gallery image 2

Published customer image:
Mouse anti Bovine interleukin-4 antibody used for the detection of IL-4 expressing cells in yellow cattle and water buffalo following schistosome infection by flow cytometry.
Image caption:
Percentage of IFN-? and IL-4 positive cells detected by flow cytometry pre and post infection. yellow cattle; (B) water buffalo. Level of significance when compared to water buffalo group at the same time, *p < 0.05.

Yang J, Fu Z, Feng X, Shi Y, Yuan C, Liu J, Hong Y, Li H, Lu K, Lin J. Comparison of worm development and host immune responses in natural hosts of Schistosoma japonicum, yellow cattle and water buffalo. BMC Vet Res. 2012 Mar 13;8:25.

Enlarge
  • IL-4 Antibody | CC303 thumbnail image 1
  • IL-4 Antibody | CC303 thumbnail image 2
  • Mouse anti Bovine Interleukin-4:RPE
  • Mouse anti Bovine Interleukin-4
  • Mouse anti Bovine Interleukin-4:FITC
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    CC303
  • Isotype
    IgG2a
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1820FF *datasheet pdfdatasheet pdf0.1 mg
    MCA1820F
    MCA1820E, F *datasheet pdfdatasheet pdf0.5 mg
    MCA1820
    MCA1820PEF *datasheet pdfdatasheet pdf100 Tests
    MCA1820PE
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Bovine Interleukin-4 antibody, clone CC303 recognizes bovine interleukin 4
    • Intended Use
    • Target Species
      Bovine
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Dogyes
      Pigyes
      Sheepyes
      Mustelidyes
      Goatyes
      Dolphinyes
      Minkyes
      Fin Whaleyes
      Horseyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
      Reconstitute with 1 ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
      IgG concentration 0.1mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
    • Storage
      Store at +4oC.

      DO NOT FREEZE

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      interleukin-4 receptor binding
      cytokine activity
      positive regulation of B cell proliferation
      B cell activation
      growth factor activity
      immune response
      negative regulation of osteoclast differentiation
      positive regulation of MHC class II biosynthetic process
      positive regulation of isotype switching to IgG isotypes
      extracellular space
      positive regulation of isotype switching to IgE isotypes
      negative regulation of apoptosis
      positive regulation of transcription from RNA polymerase II promoter
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)Neat1/5
      (1)
      Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm (Product Code BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting
      Flow Cytometry(1)1/501/100
      (1)
      Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm (Product Code BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)
      (1)
      Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm (Product Code BUF09) for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/postive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional IL-4 Antibody Formats

    Formats Clone Applications Sizes available
    IL-4 Antibody : FITC CC303 F * 0.1 mg
    IL-4 Antibody : Purified CC303 E, F * 0.5 mg
    IL-4 Antibody : RPE CC303 F * 100 Tests
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Human anti Mouse IgG2a:FITCHCA037F0.1 mgF
      HCA037F
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG2a:HRPHCA037P0.1 mgE
      HCA037P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2a Negative Control:RPEMCA929PE100 TestsF
        MCA929PE
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2a Negative ControlMCA929100 TestsF
        MCA929
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2a Negative Control:FITCMCA929F100 TestsF
        MCA929F

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry

              References

              Write your review

              You may also be interested in...

              You May Also Be Interested In...