IFN Gamma Antibody | CC302

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IFN Gamma Antibody | CC302 gallery image 1

Published customer image:Biotinylated Mouse anti Bovine interferon gamma antibody, clone CC302 (MCA1783B) used as a detection reagent in conjunction with Mouse anti Bovine interferon gamma antibody, clone CC330 (MCA2112) as a capture reagent in a sandwich ELISA to evaluate ovine ifn-&gamma responses following T. gondii infection.
Image caption:
IFN-gamma response (log10 scale) in mesenteric lymph nodes, Peyer’s Patches, popliteal LN and PBMC against GRA7, TLA and MIC3 in function of time.

From: Verhelst D, De Craeye S, Entrican G, Dorny P, Cox E. Parasite distribution and associated immune response during the acute phase of Toxoplasma gondii infection in sheep.BMC Vet Res. 2014 Dec 16;10(1):293.

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IFN Gamma Antibody | CC302 gallery image 2

Published customer image:Biotinylated Mouse anti Bovine interferon gamma antibody, clone CC302 (MCA1783B) used as a detection reagent in conjunction with Mouse anti Bovine interferon gamma antibody, clone CC330 (MCA2112)as a capture reagent in a sandwich ELISA to evaluate ovine ifn-&gamma responses following T. gondii infection.
Image caption:
Comparative IL-12 and IFN&gamma responses of neonatal and adult MLN cells following TLR agonist stimulation. Neonatal (closed circles) and adult (open squares) MLN cells were cultured in vitro with or without 12.5 µg/ml polyI:C, 0.5 µg/ml R-848 or 5 µg/ml Gardiquimod. Supernatants were harvested after 48 h of culture and ELISA was carried out for IL-12 (A) and IFN? (B) secretion. Non-specific cell stimulation was also carried out with 50 ng/ml PMA combined with 500 ng/ml ionomycin, with the supernatants assayed for IFN? detection 48 h later (C). Each circle or square represents one neonate or one adult, respectively. Medians are shown for each stimulus. Non-parametric Mann-Whitney tests were used to compare data for neonates and adults: *p=0.01; **p=0.005; ***p=0.001; ****p=0.0005.

From: Ferret-Bernard S, Remot A, Lacroix-Lamandé S, Metton C, Bernardet N, et al. (2010) Cellular and Molecular Mechanisms Underlying the Strong Neonatal IL-12 Response of Lamb Mesenteric Lymph Node Cells to R-848. PLoS ONE 5(10): e13705.

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IFN Gamma Antibody | CC302 gallery image 3

Published customer image:Phycoerythrin conjugated Mouse anti Bovine interferon gamma antibody, clone CC302 (MCA1783PE) used to assess ifn-γ expression be flow cytometry.
Identification of recombinant antibodies with specificity for bIL-2. PBMC from a cow naturally infected with M. bovis were cultured in the presence of PPD-B to allow screening of candidate bIL-2 by ICS flow cytometry. Panel A shows the histogram gating strategy used to interrogate responses in singlet, live CD4+ lymphocytes. Panel B shows the measurement of detectable IL-2 and/or IFN-γ within the CD4+ population for each of 6 candidate IL-2 antibody clones. The clone number is shown in the top left corner of each histogram and the percentage of CD4+ cell in which co-expression of IFN-γ and IL-2 could be detected is shown in the top right of each histogram. Data are representative of 1 of 2 independent experiments.

From: Whelan AO, Villarreal-Ramos B, Vordermeier HM, Hogarth PJ (2011) Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 TEM Cells in Cattle Naturally Infected with Bovine Tuberculosis. PLoS ONE 6(12): e29194.

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IFN Gamma Antibody | CC302 gallery image 4

Published customer image:
Mouse anti Bovine interferonγ antibody, clone CC302 (MCA1783) used for the identification of interferonγ expressing dog lymphocytes by flow cytometry.
Image caption:
Representative dot plots illustrating the analysis of intracellular cytokine profile in T-cell subsets. (A) Pseudocolor plot distribution of short-term in vitro cultured (control or SLA-Ag stimulated) canine whole blood sample according to cell size (Forward scatter - FSC) and granularity (Side scatter- SSC) used for lymphocyte gate selection (R1) of FSCLowSSCLow events. (B) Pseudocolor plots representing cytokines?+?(IL-17, TNF-a, IFN-?, TGF-ß and IL-4) CD4+ cells within gated lymphocytes and (C) Pseudocolor plots representing cytokines?+?(IL-17, TNF-a, IFN-?, TGF-ß and IL-4) CD8+ cells within gated lymphocytes. The frequency of cytokines+ T-cells subsets were calculated by quadrant statistics approach and first reported as percentage of gated lymphocytes prior to the calculation of the SLAg/Control indexes.

From: Costa-Pereira C, Moreira ML, Soares RP, Marteleto BH, Ribeiro VM, França-Dias MH, Cardoso LM, Viana KF, Giunchetti RC, Martins-Filho OA, Araújo MS. One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasis. BMC Vet Res. 2015 Apr 11;11(1):92.

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IFN Gamma Antibody | CC302 gallery image 5

Published customer image:
Mouse anti bovine interferon gamma antibody, clone CC302 used for the measurement of the gamma interferon response to M. avium infection in a caprine model by ELISA in conjunction with biotinylated Mouse anti Bovine interferon γ antibody, clone CC330 as a capture reagent.
Image caption:
Time course and intensity of antigen-induced (Johnin, 4 µg/mL) IFN-γ response (B) of inoculated and control goats. Box and Whisker Plot represents median value, 25% and 75% percentiles (box), range, outlier values (?), and extreme values (*). Different letters indicate significant differences between groups (Mann–Whitney-U test, P?≤?0.05): a – V1 vs. V2, b – V1 vs. V3, c – V1 vs. V4, d – V2 vs. V3, e – V2 vs. V4, f – V3 vs. V4.

From: Köhler H, Soschinka A, Meyer M, Kather A, Reinhold P, Liebler-Tenorio E. Characterization of a caprine model for the subclinical initial phase of Mycobacterium avium subsp. paratuberculosis infection. BMC Vet Res. 2015 Mar 24;11(1):74.

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IFN Gamma Antibody | CC302 gallery image 6

Published customer image:
Mouse antiBovine Ifn γantibody, clone CC302 (MCA1783) and Mouse anti Bovine IL-4 antibody, clone CC303 (MCA1820) used to evaluate cytokine expression by canine peripheral blood cells following short term in vitro stimulation and culture by flow cytometry.
Image caption:
Internal controls to validate the cross-reactivity of anti-human cytokine mAbs with canine cytokines following short-term whole blood cultures in vitro. Non-specific binding was monitored by using isotype matched reagents (top panels). Blocking strategy with host serum was also applied (bottom panels). Controls of reference cross-reactive mAbs (anti-bovina IFN-? and IL-4) were also included. Dot plot distribution graphs of empty channel (FL4 fluorescence) versus PE-emission channel (FL2) were used to quantify the percentage of cytokine+ lymphocytes in unstimulated controls (left panels) and PMA + LPS stimulated cultures (right panels). Only Q1 and Q4 quadrants are represented. Gray background highlights the reactivity above 0.5 % in the quadrant Q1

From: Moreira ML, Dorneles EM, Soares RP, Magalhães CP, Costa-Pereira C, Lage AP, Teixeira-Carvalho A, Martins-Filho OA, Araújo MS.
Cross-reactivity of commercially available anti-human monoclonal antibodies with canine cytokines: establishment of a reliable panel to detect the functional profile of peripheral blood lymphocytes by intracytoplasmic staining.
Acta Vet Scand. 2015 Sep 11;57:51.

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IFN Gamma Antibody | CC302 gallery image 7

Published customer image:
RPE conjugated mouse anti Bovine Ifn-γ antibody, clone CC302 (MCA1783PE) used to evaluate ifn-γ expression in canine lymphocytes by flow cytometry.
Image caption:
CD4+ T cells from Ech_0660 mutant vaccinated and wild-type E. chaffeensis infected animals produce IFNγ in response to E. chaffeensis antigen. PBMC from dogs vaccinated with the Ech_0660 mutant and challenged with wild-type E. chaffeensis (groups 1–3, as in Fig 2) were cultured for 5 days at 4x106 cells/mL in the presence or absence of 10 μg/mL E. chaffeensis host-cell free lysate grown in the tick ISE6 cell line. On day 5, brefeldin A was added for the last 6 hours of culture. CD4+ T cells were stained for intracellular expression of IFNγ and analyzed by flow cytometry. (A) Representative flow plots from animals in groups 1, 2 and 3, gated on total live cells and total CD3+CD4+ T cells. (B) The percentage of IFNγ+ cells of total CD4+ T cells in the blood measured over the course of the experiment. Background (mock stimulated) IFNγ production was subtracted, and results represent change over mock.

From: McGill JL, Nair ADS, Cheng C, Rusk RA, Jaworski DC, Ganta RR (2016)
Vaccination with an Attenuated Mutant of Ehrlichia chaffeensis Induces Pathogen-Specific CD4+ T Cell Immunity and Protection from Tick-Transmitted Wild-Type Challenge in the Canine Host.
PLoS ONE 11(2): e0148229.

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IFN Gamma Antibody | CC302 gallery image 8

Published customer image:
RPE conjugated Mouse anti Bovine Ifn-γ antibody, clone CC302 (MCA1783PE) used to evaluate ifn-γ expression in canine lymphocytes by flow cytometry.
Image caption:
CD8+ T cells from Ech_0660 vaccinated and wild-type E. chaffeensis infected animals proliferate and produce IFNγ in response to E. chaffeensis antigen. CD8+ T cell proliferation and IFNγ production were measured using similar approaches as in Figs 2 and 3. PBMC from dogs in groups 1–3 were cultured for 5 days at 4x106 cells/mL in the presence or absence of 10 μg/mL E. chaffeensis host-cell free lysate. On day 5 of culture, CD8+ T cells were analyzed by flow cytometry for (A) proliferation as measured by Cell Trace Violet dilution; and (B) intracellular production of IFNγ. The frequencies of responding CD8+ T cells were measured over the course of the experiment. Results were gated on total live cells and total CD3+CD8+ T cells. Background (mock stimulated) proliferation or IFNγ production was subtracted and results represent change over mock.

From: McGill JL, Nair ADS, Cheng C, Rusk RA, Jaworski DC, Ganta RR (2016)
Vaccination with an Attenuated Mutant of Ehrlichia chaffeensis Induces Pathogen-Specific CD4+ T Cell Immunity and Protection from Tick-Transmitted Wild-Type Challenge in the Canine Host.
PLoS ONE 11(2): e0148229.

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  • Mouse anti Bovine Interferon Gamma:Alexa Fluor®647
  • Mouse anti Bovine Interferon Gamma:RPE
  • Mouse anti Bovine Interferon Gamma:FITC
  • Mouse anti Bovine Interferon Gamma:Biotin
  • Mouse anti Bovine Interferon Gamma:Alexa Fluor®488
  • Mouse anti Bovine Interferon Gamma
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  • Product Type
    Monoclonal Antibody
  • Clone
    CC302
  • Isotype
    IgG1
6 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1783FF*datasheet pdfdatasheet pdf0.1 mg
    MCA1783F
    MCA1783BEdatasheet pdfdatasheet pdf0.25 mg
    MCA1783B
    MCA1783E, F*datasheet pdfdatasheet pdf0.5 mg
    MCA1783
    MCA1783PEF*datasheet pdfdatasheet pdf100 Tests
    MCA1783PE
    MCA1783A647F*datasheet pdfdatasheet pdf100 Tests/1ml
    MCA1783A647
    MCA1783A488F*datasheet pdfdatasheet pdf100 Tests/1ml
    MCA1783A488
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Bovine IFNγ antibody, clone CC302, recognizes bovine interferon-gamma, a 143 amino acid cytokine with potent activating, antiviral and anti proliferitive properties, produced as a pro-peptide with an additional 23 amino acid N-terminal signal peptide sequence having a molecular weight of ~20 kDa. IFNγ is predominantly secreted by activated T lymphocytes in response to specific mitogens as a result of infection (Rhodes et al. 2000).

      Mouse anti bovine γ inerferon antibody, clone CC302 has been demonstrated to be reactive to a number of mammalian species including human, sheep, dog, pig, goat and mink (Pedersen et al. 2002). Clone CC302 has been successfully used for the evaluation of γ interferon levels in the sera of calves naturally infected with M. avium. subsp paratuberculosis (Appana et al. 2013) as a detection reagent using an ELISA.
    • Intended Use
    • Target Species
      Bovine
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Humanyes
      Pigyes
      Dogyes
      Horseyes
      Sheepyes
      MustelidExpected from Sequence
      Goatyes
      Dolphinyes
      Ferretyes
      Minkyes
      Fin Whaleyes
      Rabbityes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Biotin - liquid
      Purified IgG conjugated to Alexa Fluor 488 - liquid
      Purified IgG - liquid
    • Reconstitution
      Reconstitute with 1 ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.05mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 0.5 mg/ml
      IgG concentration 0.05mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody.
      Store at +4oC.

      DO NOT FREEZE

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      12 months from date of reconstitution
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      immune response
      extracellular space
      interferon-gamma receptor binding
      response to virus
      cytokine activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)1/101/100
      (1)
      Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm (Product Code BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)Neat1/10
      (1)
      Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm (Product Code BUF09) for this purpose.

      For use on Equine samples Bio-Rad recommend MCA1783F
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)1/10
      (1)
      Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm (Product Code BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      ELISA5ug/ml
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)1/201/200
      (1)
      Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm (Product Code BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry(1)1/1001/500
      (1)
      Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm (Product Code BUF09) for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
      Biotinylated mouse anti bovine IFNγ, clone CC302, may be used as the detection reagent in a sandwich ELISA with purified mouse anti bovine IFNγ, clone CC330, as the capture reagent and recombinant bovine IFNγ as the standard.
    • ELISA
    • ELISA
      Biotinylated mouse anti bovine IFNγ, clone CC302, may be used as the detection reagent in a sandwich ELISA with purified mouse anti bovine IFNγ, clone CC330, as the capture reagent and recombinant bovine IFNγ as the standard.
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional IFN Gamma Antibody Formats

    Formats Clone Applications Sizes available
    IFN Gamma Antibody : Purified CC302 E, F* 0.5 mg
    IFN Gamma Antibody : Alexa Fluor® 647 CC302 F* 100 Tests/1ml
    IFN Gamma Antibody : Alexa Fluor® 488 CC302 F* 100 Tests/1ml
    IFN Gamma Antibody : Biotin CC302 E 0.25 mg
    IFN Gamma Antibody : FITC CC302 F* 0.1 mg
    IFN Gamma Antibody : RPE CC302 F* 100 Tests
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:Alexa Fluor® 647MCA928A647100 Tests/1mlF
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:FITCMCA928F100 TestsF
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:Alexa Fluor® 488MCA928A488100 Tests/1mlF
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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