CD62L Antibody | CC32

//www.bio-rad-antibodies.com/cow-bovine-cd62l-antibody-cc32-mca1649g.html?RenderPageSpecific=1
CD62L Antibody | CC32 gallery image 1

Published customer image:
Mouse anti Bovine CD62L antibody, clone CC32 used for the evaluation of L-selectin expression on PBMC from M. bovis cattle by flow cytometry.
Image caption:
Characterisation of memory markers reveals a characteristic TEM phenotype for multifunctional CD4+ cells in naturally infected cattle. PBMC from naturally M. bovis infected cattle were stimulated with PPD-B and stained for production of cytokine by ICS flow cytometry. Histograms were first gated on singlet, live lymphocytes and gating strategies applied to allow phenotyping of cells for expression of CD44, CD45RO and CD62L. The quadrant gate defining expression of CD44 and CD62L (top panel), CD45RO and CD62L (middle panel) or CD44 and CD45RO (bottom panel) was determined using the total live-lymphocyte population, as shown for each surface marker combination in the histograms on the far left of the panel. This quadrant gating strategy was used to determine the surface phenotype of total CD4+IFN-γ+, CD4+IL-2+, CD4+TNF-α+ and triple functional CD4+ cells, as shown in the other histograms. The percentage of cytokine producing CD44hiCD62Llo, CD45RO+CD62Llo and CD44hiCD45RO+ is shown for each of these CD4+ populations. The data is from one representative animal of 5 analysed.

From: Whelan AO, Villarreal-Ramos B, Vordermeier HM, Hogarth PJ (2011) Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 TEM Cells in Cattle Naturally Infected with Bovine Tuberculosis.
PLoS ONE 6(12): e29194.

Enlarge
  • CD62L Antibody | CC32 thumbnail image 1
  • Mouse anti Bovine CD62L:FITC
  • Mouse anti Bovine CD62L
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    CC32
  • Isotype
    IgG1
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1649FFdatasheet pdfdatasheet pdf0.1 mg
    MCA1649F
    MCA1649GFdatasheet pdfdatasheet pdf0.25 mg
    MCA1649G
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Bovine CD62L antibody, clone CC32 recognizes bovine L-selectin, also known as CD62L, Leukocyte-endothelial cell adhesion molecule 1, LECAM-1 or Lymph node homing receptor. Bovine CD62L is a 370 amino acid ~90kDa, single pass type I transmembrane glycoprotein bearing a single C-type lectin domain, an EGF-like domain and two Sushi domains (UniProt: P98131).

      Immunoprecipitation of peripheral blood mononuclear cell lysates with Mouse anti Bovine CD62L antibody, clone CC32 reveals a molecule of ~90 kDa when run on polyacrylamide gels under reducing conditions, slightly larger than the murine and human CD62L homologues. Bovine CD62L is expressed on subpopulations of T-lymphocytes expressing CD2, CD4 and CD8. WC1 positive γ/δ T cells also express CD62L as do a subpopulation of WC3+ve B-lymphocytes and all peripheral blood monocytes (Howard et al. 1992).

      Mouse anti Bovine CD62L antibody, clone CC32 has also been used successfully for the identification of CD62L on ovine peripheral blood cells by flow cytometry (Halliday et al. 2005).
    • Intended Use
    • Target Species
      Bovine
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Sheepyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
    • Immunogen
      Bovine lymphocytes
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the NS1 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CD62L Antibody Formats

    Formats Clone Applications Sizes available
    CD62L Antibody : FITC CC32 F 0.1 mg
    CD62L Antibody : Purified CC32 F 0.25 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      HCA036P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:FITCMCA928F100 TestsF
        MCA928F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF
        MCA928

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry
                Immunohistology - Frozen

              References

              Write your review

              You may also be interested in...